Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,375,737	G→A	A259T (GCC→ACC)	ycjQ →	D‑guloside 3‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,375,737	0	G	A	97.6%	103.7 / NA	41	A259T (GCC→ACC)	ycjQ	D‑guloside 3‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/29);  minor base T (1/0);  total (12/29)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGCGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTGCCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCAT  >  NC_000913/1375494‑1375979                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   aaaCGCGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGc                                                                                                                                                                                                                                              <  1:77242/250‑1 (MQ=255)                            ggcggcGACCCTATCGCCCATCGCGTTGTCACTGCCCGTCTCCTAGGCGTGG‑TTTCTCGCCTTAACCCCATCGGCACTGATGTAAGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACCCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGTGGCACCATCCCCTATGTCGCGTGGACCAAGACGTTTGCCGAAGGTTTTAACCTCGGAcg                                                                                                                                                                                                                  <  2:97510/251‑1 (MQ=255)                            ggCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGAc                                                                                                                                                                                                                   <  1:492411/250‑1 (MQ=255)                              cGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCCCCATCCCCTATGTCGCGTTTACCAACCCGTTTGCCGAAGGGTTTAACCTCGGGcgcg                                                                                                                                                                                                                >  2:7687/1‑251 (MQ=255)                               gTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTCACCCCATCGGCACTGATGTAGGTAACGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGCCGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCCCCCTCTCCTATGTCGCGTTTACCAAGCCGTTTGccagtcgggagagcacacgtctgc                                                                                                                                                                                                                >  1:312288/1‑228 (MQ=255)                                 cGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAg                                                                                                                                                                                                             <  1:193057/251‑1 (MQ=255)                                                   cTGTGATATTGCCCGTAGCCACGGCGCGGCTTTCT‑GCCTTCACCCCATAGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGATTTAACCTCGGCAGCGAAGCGCATTTCAATAAAGcc                                                                                                                                                                                            >  1:431813/1‑250 (MQ=255)                                                          attggcagTCGCCACGGCGCGGTTTTCT‑GCCTGAACCACATCGGCACGGATGTAGGTAAAGATATCACAACGCGGACCGTCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTACGCGGTCTGGCTTATGGCGGTACCAGCCCCTATGTCTCGTTTACCAATCCGTTTGCCGAAGGGTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCATAATTg                                                                                                                                                                                     <  2:428671/243‑1 (MQ=255)                                                          atTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAACCGGTTGCCGAAGGTTTTAACCTTGGAAGCGGAGCGCATTTCAATAACGCCAAAATTGt                                                                                                                                                                                    >  1:436264/1‑251 (MQ=255)                                                            tGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGtc                                                                                                                                                                                   <  2:226210/250‑1 (MQ=255)                                                             gCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGtctt                                                                                                                                                                                 <  1:320761/251‑1 (MQ=255)                                                                   cGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTtctctc                                                                                                                                                                            <  1:28480/250‑1 (MQ=255)                                                                    gCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTcgc                                                                                                                                                                          <  2:67202/251‑1 (MQ=255)                                                                      cACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCACCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGGTTTAAACCCGGGAGGGAAGCGACGTTCAATACAGCCAAAATTGTCTTCTCTcgcgg                                                                                                                                                                        >  1:261597/1‑250 (MQ=255)                                                                        cGGCGCGGATTTCT‑GCATTAACCCCATCGGCACTGATGGAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGCCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTTCCACACAGTTTACAATCGGGTTTAACATCGGACGCGCAGCGCATTTCAATAACGCCAAAATTGTATtctctaacgctt                                                                                                                                                                      >  2:54970/1‑244 (MQ=255)                                                                                 tttCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCCAGCCGTTTGCCGAAGGATTTAACCACCGACGCGAAACGCATTTCAATAACGCCAAACTTTTCTTCTCTCGCGCGTgaccccac                                                                                                                                                              >  2:346578/1‑243 (MQ=255)                                                                                  ttCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGcgaac                                                                                                                                                             <  1:424331/250‑1 (MQ=255)                                                                                       gCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGcgaaccgaa                                                                                                                                                         <  1:419364/250‑1 (MQ=255)                                                                                          ttAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTAACAAGCCGTTTGCCGAAGGTTTTAACCTCGGACACGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCCAACCGAACCCg                                                                                                                                                     >  1:140104/1‑251 (MQ=255)                                                                                              ccccATCGTCACTGATGTAGGTAAAGAGATAAAAACGCTGACCGGCAAGCAGGGGGCCGATGTGATTATCGAATCCAGCGGCGACGCCGACGCGCTGCAATCTGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGCAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGATCCGATCCCGGAtt                                                                                                                                                 <  2:503038/251‑1 (MQ=255)                                                                                                    cGGCACTGATGTAGGTAAATAGATCAAAACGCGGACCGGCAAGCAGGGTGCCGATGTGATTATCGAACCCAGCGGCTACGCCGATGCGCTGCAATCGGCGCTCCGCGGTCTGGCTGATGGCGTCACCATCTCCGATGTCGCGTTTACCAAGTCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTACAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCcg                                                                                                                                            <  2:18819/250‑1 (MQ=255)                                                                                                         cggagttAGGTAAAGAGAGCAAAACGCGTACCGGCAAGCAGTGTGACGATTTGATTATCGAATGCAGCGGCTACGACGATGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCTCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCTAACCCGGATTATCCGCGCTgg                                                                                                                                      <  2:502605/245‑1 (MQ=255)                                                                                                          tGATGTAGGGAACGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCACTCGGCGCTCCGCGGTCTGGCTTATGGCGGAACCCTCTCCTATGTCGCGTTTACCCAGCCGGTTTCAGCAGGTTTTAACCTCGGACGCGAAGCGCATTTCCATACCGCCACAATTTTCTTCTCTCACGCGTTCAGCgacacgaacctcgattctcaccgcgcg                                                                                                                                      >  2:142/1‑233 (MQ=255)                                                                                                                  gTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAg                                                                                                                             <  1:165261/251‑1 (MQ=255)                                                                                                                            cAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGGGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTga                                                                                                                     >  1:344204/1‑249 (MQ=255)                                                                                                                                 cGCTGGCCGGCAAGCAGGGGTCCGATGTGATTATCGAAACCCGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCCCCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAAcc                                                                                                              <  2:264567/251‑1 (MQ=255)                                                                                                                                 cGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAAcc                                                                                                              <  1:85217/251‑1 (MQ=255)                                                                                                                                   cTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCCCGGACGCGAAGCGCCTTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGCTTATCCGCGCTGTAGCCGCAAGCGTATTTGAGAAAcccg                                                                                                            >  2:484495/1‑249 (MQ=255)                                                                                                                                          gcatgCAGGGTGCCGATGGGATTATCGAAACCAGCGGCTACGCCGACGCGTGGCAAGCGGCGCTCCGCGGGCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGTCGTTTGCCGAAGGTTTTAACCTCGGACGTGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGGGCAGCGAACCGAGCCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGaa                                                                                                     <  2:495217/247‑1 (MQ=255)                                                                                                                                           cAAGCAGGGTGCCGATGTGATGATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCGCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAAc                                                                                                    <  2:502703/251‑1 (MQ=255)                                                                                                                                                    ggcagATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCCCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCggatga                                                                                           <  2:226059/247‑1 (MQ=255)                                                                                                                                                      ccGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAAGCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAAc                                                                                         <  1:376121/251‑1 (MQ=255)                                                                                                                                                             gATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTAt                                                                                   <  1:78051/250‑1 (MQ=255)                                                                                                                                                                              gCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTa                                                                 <  1:7687/251‑1 (MQ=255)                                                                                                                                                                               gtACGCCGACGCGCTGCAATCGGCGTTATGCGGTATTGCTTATGGAGGCACCATCTCCTATGTCGCGTTTACCAAGACGTGTGCCGAAGGTTGTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTGCTCCCGCGCGTGCAGCGAACCGAACCCGGATTAACCGCGCTGGAGCCGCAAGCGGAGTGAAGAAACCTGATGGGAACTGATGATGAACGGTTATCTCAATTGCGAAGATTTaa                                                                <  2:333615/250‑1 (MQ=255)                                                                                                                                                                                         gcgcTGCAATCGGCTCCCCGTGGTCGGGCTTACGGCGGCCCCCTCTCCTATGTCGCGTTTACCAGGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAAGTGTCTTCGCGCGCGCGTGCAGCGAACCGAACCCTGATTATCCGCGCGGTAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAGGATTTAATCGACCCggt                                                      <  2:486655/251‑1 (MQ=255)                                                                                                                                                                                                              ggTCTTGCTTATGGCGGCCCCAGCTCCTGTGTCGCGTTTACCAAGCCGTTTGCCGAATGTTTTAACCTCGGACGCGAAGCTCATTTCAATAACGCCAAAAGTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCGCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGccc                                 <  1:485098/251‑1 (MQ=255)                                                                                                                                                                                                                           gcggcCCCATCTCCTATGTCGCGTTTACCAAGCCGGTTGCCGAAGGTTTTAACCTCGGACGCGAATCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGTAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAATATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATg                    <  2:452262/251‑1 (MQ=255)                                                                                                                                                                                                                             ggcACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGACAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGGTTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGACAGCTATATGc                   >  1:468691/1‑250 (MQ=255)                                                                                                                                                                                                                                          aTGTCGCGTTTAACAAGCCGTTTGCTTAAGTTTGTAACCTAGGACGCGAAGCGCATTTCAATAACGCCTACCTCGACCTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGTCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAATCTATAAGCAgcacgtcgtcca      <  2:129419/250‑12 (MQ=255)                                                                                                                                                                                                                                             gcgcgTTTACCAAGCCGTTTTCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGGCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCAt  <  2:129410/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   AAACGCGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCT‑GCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTGCCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCAT  >  NC_000913/1375494‑1375979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑