Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	95.7 / NA	34	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/17);  total (17/17)

GAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAT  >  NC_000913/1306496‑1306972                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             gAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACACGTTGCCCGATTGCCGGTACAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGAAACGCCGTTTCTTg                                                                                                                                                                                                                                    >  1:377483/1‑251 (MQ=255)           ccTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTc                                                                                                                                                                                                                          <  1:409221/251‑1 (MQ=255)              attgtgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGCCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTGCTTGCCTGAAAGTCg                                                                                                                                                                                                                         <  1:387810/245‑1 (MQ=255)                 atgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAACACCATCCAGTTACTGGAAGGGGACTTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAGACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCg                                                                                                                                                                                                                    >  1:278257/1‑251 (MQ=255)                          tccACAACCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATCCCTGTTCCGGACCTGGAGAAGAACAAAACCAGCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTGCCGTACCCGTGTCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCTTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTaa                                                                                                                                                                                                              <  2:383201/247‑1 (MQ=255)                                            cTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTCAAAACAATAAGGGCTGACAGtt                                                                                                                                                                                         >  1:415774/1‑251 (MQ=255)                                                 cccAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAg                                                                                                                                                                                    <  1:457711/250‑1 (MQ=255)                                                        cATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGGGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                             >  2:458339/1‑251 (MQ=255)                                                            gATGGCGGCAGTCTCCCA‑ACCTGATCCGGCTCGTGAGAAAAACAAAACCATCCAGTTACTGGAAGTGGAATTACCGTCGCCGATCTT‑CCCGCCTCCCGGTTGTGTTTTCCGTACCAGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACTCGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCAc                                                                                                                                                                         <  1:45405/251‑1 (MQ=255)                                                                  ggCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGCCACGCCGTTTCTTGCCTGaaa                                                                                                                                                                                                                             >  2:479174/1‑193 (MQ=255)                                                                  ggCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGaaa                                                                                                                                                                                                                             <  1:479174/193‑1 (MQ=255)                                                                            cATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGattat                                                                                                                                                          >  1:294378/1‑251 (MQ=255)                                                                             aTACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGCAGGGGAATTACCGACGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGAGattatt                                                                                                                                                         >  2:121803/1‑251 (MQ=255)                                                                                ccTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGAttatttat                                                                                                                                                      >  1:297538/1‑251 (MQ=255)                                                                                           cctttccgatccaaaaaCCAACAAGTTACGGGAAGGGGAATTCCCTTCGCCGATCAA‑TCCGCCGTCCGTTTGGGTTTGCCGTACCCGTGGCCCGATGGCCGGTCCAGAGTGCGCAAAATCACGTCCTGTTCTGGAGGGGGGTTTCAGACACGCCGTTTCTTGCCTGAAAGTTGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGGCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgat                                                                                                                                           <  2:260892/238‑1 (MQ=255)                                                                                              ggagatcaaCAAAACCATCCATTTACGGAAAGGGGAATTTCCGCCGCCGATCAC‑CCCGCCTTCCGGTTGTGTTTTCCGTACCTGTTGCCCGATTGCCGGTCTAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAg                                                                                                                                             <  2:351732/239‑1 (MQ=255)                                                                                                    gaaCAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGc                                                                                                                                   >  1:362844/1‑250 (MQ=255)                                                                                                     aaCAAAACCACCCAGTTACTGGAAGGGGAATTACCGACGCCGATCAC‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGGCCAGAGGGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCaa                                                                                                                                 <  2:82728/251‑1 (MQ=255)                                                                                                       cAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGCTTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTATCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgat                                                                                                                                           >  1:260892/1‑239 (MQ=255)                                                                                                              aTCCAGTGACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGa                                                                                                                        <  1:422162/251‑1 (MQ=255)                                                                                                                   ggttcgggAAGGGGAATTACCGTCGCCGA‑CAATCCCCCCTCCCGTTGGGGTTTCCCGTCCCCGTTGCCGGATGGCCGGTCCAGAGTGCGCCAAATCACGTCCGGTTCTGGATGGTAGTTCCAGACACGCCGTTTCTTGCCTGAAAGGCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTGTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCACAGTGTGTGATCttt                                                                                                                   <  2:297538/245‑1 (MQ=255)                                                                                                                                              gATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGAATAACAGAATGCTGGCaa                                                                                         >  2:445605/1‑250 (MQ=255)                                                                                                                                                        cccTTCCGGTTGTGTTTTCCGTACCCGTTGCCCTATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTCCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGtt                                                                               <  1:484634/250‑1 (MQ=255)                                                                                                                                                         cctcccGGTTGTGTTTTCCGATCCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTg                                                                              <  2:114654/247‑1 (MQ=255)                                                                                                                                                           ttCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAt                                                                            >  1:297242/1‑251 (MQ=255)                                                                                                                                                               ggTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGCTTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTc                                                                         >  1:194782/1‑250 (MQ=255)                                                                                                                                                                gTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTGTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCAc                                                                       <  1:137924/251‑1 (MQ=255)                                                                                                                                                                 ttGTGTTTTCCGTACACGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACAAGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGAGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCAGCATAACAGAATGAGGGCACAAAAATCGTTGATTTCAcc                                                                      >  2:498316/1‑251 (MQ=255)                                                                                                                                                                     gtTTTCCGTACCCGTTGCCCGTTTGCCGGTCAAGAGTGCGCCAAAACACGGCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGagtt                                                                          <  2:139232/243‑4 (MQ=255)                                                                                                                                                                     gtTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAttt                                                                          >  1:139232/1‑243 (MQ=255)                                                                                                                                                                              ccccGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGCGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTcc                                                         <  1:121803/250‑1 (MQ=255)                                                                                                                                                                                            tgcaggTCCAGAGTGCGCCAAATCACGTCCTGTTCGGGAGGTGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATca                                            <  2:415774/246‑1 (MQ=255)                                                                                                                                                                                                                      gTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAGAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATCCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGACCGGGTTc                  >  2:183664/1‑250 (MQ=255)                                                                                                                                                                                                                                     ggAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAt  >  1:477987/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             GAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTC‑CCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAA‑CCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAT  >  NC_000913/1306496‑1306972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑