Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,663	C→T	S533F (TCT→TTT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,663	0	C	T	100.0%	150.9 / NA	49	S533F (TCT→TTT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/28);  total (21/28)

CGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACT  >  NC_000913/1252416‑1252906                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cggcgTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGCCCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTtg                                                                                                                                                                                                                                                  >  2:307423/1‑251 (MQ=255)   gcgTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGCCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTtgtg                                                                                                                                                                                                                                                <  1:107515/251‑1 (MQ=255)        tAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACCAATTACGCAGTCTTGAAAAACGCTTCTCTACGAGTCTGAAAAGTGATCACGATGCCCTCGCTCGCCTGGTTTTTTGTGCAttg                                                                                                                                                                                                                                           >  2:111659/1‑249 (MQ=255)                   gATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGTAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCTCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACg                                                                                                                                                                                                                                 <  1:502176/250‑1 (MQ=255)                            gggATTGCAACAACGCCCGCGGTCCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCGGTATTACGCGCTGCATGCAGTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACt                                                                                                                                                                                                                       <  1:59353/249‑1 (MQ=255)                              gATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACttt                                                                                                                                                                                                                     <  1:375619/251‑1 (MQ=255)                                  gCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACATAACCGTTTTAGTCGCCAGTTGTTTTACGCGTGGCATGCATTTGAAATGCCCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACa                                                                                                                                                                                                                  <  1:18517/250‑1 (MQ=255)                                         ccaCCGCGCACCGCGTAATTCTGTTGTAACAAAATGGTTTACGTCGCCAGCTGTATTCCGCCCTGCGTGCATTTGAATTTCCCATCCCGCCCCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTATTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATc                                                                                                                                                                                                          <  1:149331/249‑1 (MQ=255)                                          gACCGCGGAACACGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTAAGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGCTGCCCTCGCTCGCCTGGTTTTTTGAGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCCTCg                                                                                                                                                                                                         >  2:464518/1‑251 (MQ=255)                                                                   gAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATa                                                                                                                                                                                 >  1:504049/1‑250 (MQ=255)                                                                             gTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGGGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGAAtt                                                                                                                                                                      >  1:373477/1‑251 (MQ=255)                                                                               tttAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGGGATAACGGGCGAATTcg                                                                                                                                                                    >  2:299709/1‑251 (MQ=255)                                                                                   gTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTc                                                                                                                                                                >  1:388998/1‑251 (MQ=255)                                                                                      ccgagatgtgttACGCGTCGCATGCAGTTTAAAGTACCATCCCGCCTCTGCGTATGCGGCTTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGTAGACTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGGCTTCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGt                                                                                                                                                             <  1:170481/241‑1 (MQ=255)                                                                                       ccagctgtttgccgggcTGCAGGCTTTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTg                                                                                                                                                            <  2:57413/236‑1 (MQ=255)                                                                                       ccAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGGGTAGTGATAACGGGCGCATTAGCGTCAGTg                                                                                                                                                            >  1:294558/1‑251 (MQ=255)                                                                                          gCTGTATTACGGGCGGCATGCAGTATAAATACCCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCATCGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGAt                                                                                                                                                          <  1:34454/250‑1 (MQ=255)                                                                                           cTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGAttt                                                                                                                                                        >  1:178728/1‑251 (MQ=255)                                                                                            tGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTg                                                                                                                                                       >  1:222929/1‑251 (MQ=255)                                                                                                  aCGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGa                                                                                                                                                  >  1:484812/1‑250 (MQ=255)                                                                                                      gcTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATc                                                                                                                                             >  2:322127/1‑251 (MQ=255)                                                                                                       cggcatgcatTTGAAATAACCATCCCGCCTCAGCGTATGCGGCGTGGCAGCATTCCGGCGCAGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCt                                                                                                                                            <  2:16777/249‑1 (MQ=255)                                                                                                             gcatTAGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTAc                                                                                                                                      <  1:166118/251‑1 (MQ=255)                                                                                                                                                     gcagcaTTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTcc                                                                                              >  1:84082/1‑251 (MQ=255)                                                                                                                                                          aTTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAg                                                                                         <  1:322127/251‑1 (MQ=255)                                                                                                                                                                 cgcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCa                                                                                  <  1:157786/251‑1 (MQ=255)                                                                                                                                                                  gcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGGGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAt                                                                                 <  1:336285/251‑1 (MQ=255)                                                                                                                                                                  gcTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAATTTACAGCGTCATCGCGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGCTGTCAGCGCCCCCCGCCTTTCCACCAGTCTTTCAt                                                                                 >  2:429736/1‑251 (MQ=255)                                                                                                                                                                     ggggAATAACAAATTACGCAGGCTTGAAAAACGCTGCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCAttt                                                                               <  2:178728/247‑1 (MQ=255)                                                                                                                                                                     ggggAATAAAAAATTACGCAGTCTTGAAAAACGCTTCTCTACGAGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAGTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCATTTCCACCAGTCTGTCATTTg                                                                              <  2:282528/248‑1 (MQ=255)                                                                                                                                                                     ggTGAATAACAAATTTCTCAGTCTTGAAAAGCGCTTCTCTACGCGGCTGAAAAGTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTTATCGGGAATCTTGCTCTGATTAGTGAGAACGGGCGCATTCGCTTCAGTGATTTGCTGGAACATCTGTTTACCGAGCAGTCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTg                                                                              <  2:6633/251‑1 (MQ=255)                                                                                                                                                                         aaTAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAAc                                                                                                                                                                               >  2:144065/1‑150 (MQ=255)                                                                                                                                                                         aaTAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAAc                                                                                                                                                                               <  1:144065/150‑1 (MQ=255)                                                                                                                                                                          aaacaaaaTTACCCAGTCTTGAAAAACGCTTCTTTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGaa                                                                         <  2:11796/246‑1 (MQ=255)                                                                                                                                                                              cAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCAGCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTg                                                                     <  1:420726/251‑1 (MQ=255)                                                                                                                                                                                  ttACGCAGTATTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGAATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATATTGCACTGAGTAGTGATAACGGGCGCATTCGCGACAGTGCTTTGCCGGAACATCTGTTTACCGGGCAGCCGACAGATGATGTCAGCGCCACCCGCCTTTCTACCAGTCTGTCATTTGCGGAAGTTGaaa                                                                  >  1:503343/1‑250 (MQ=255)                                                                                                                                                                                   tACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATCACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCTCCAGTCTGTCATTTGCGGAAGTTGaaaa                                                                 <  1:151241/250‑1 (MQ=255)                                                                                                                                                                                    aCGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGaacacg                                                               >  2:72584/1‑247 (MQ=255)                                                                                                                                                                                         gTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCATCGCTCGCCTGGTCTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCa                                                          <  2:161819/251‑1 (MQ=255)                                                                                                                                                                                          tcgtGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGACCTCGAGCGCCTGGTTTTTTGTGCATGGCCAGGCCACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTAAGTGATTTTCCGGAACATCTGTTTACCGAGCAGGCGCCAGATGATTTCAGCGCCACCCGCCTTTCCACCAGTCTGTCTTTAGCGGAAGTTgcaacatgggcac                                                         >  2:201821/4‑239 (MQ=255)                                                                                                                                                                                              gAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAATATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAatta                                                      <  1:464518/250‑1 (MQ=255)                                                                                                                                                                                                  aaCGCTTCTCCACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAAc                                                 <  1:258064/251‑1 (MQ=255)                                                                                                                                                                                                                        aaTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACACGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACCGGCGGTc                           >  2:58558/1‑251 (MQ=255)                                                                                                                                                                                                                         aTTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCg                          <  1:132319/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccTCGCTCGACTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTTATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGt            <  1:276092/251‑1 (MQ=255)                                                                                                                                                                                                                                             ccgcCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCtt      <  2:143634/250‑1 (MQ=255)                                                                                                                                                                                                                                              cgcCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTTCGGAGATGTCgggggt     >  2:100372/1‑247 (MQ=255)                                                                                                                                                                                                                                              cgcCTGGTTTTTTGGGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCgg         <  1:100372/247‑1 (MQ=255)                                                                                                                                                                                                                                                 cTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAAAGTTGGCTTTACt  >  1:315859/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACT  >  NC_000913/1252416‑1252906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑