Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,244,908	C→T	L61L (CTG→TTG)	ymgE →	PF04226 family protein YmgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,244,908	0	C	T	100.0%	83.7 / NA	27	L61L (CTG→TTG)	ymgE	PF04226 family protein YmgE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/18);  total (9/18)

ACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTT  >  NC_000913/1244669‑1245150                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      aCTTAGAGCTTTGATGTAAGGATTGCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCtt                                                                                                                                                                                                                                            <  2:1488/250‑1 (MQ=255)    gagagCGTTGATGTAAGCATTTCTTGTGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGGCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCt                                                                                                                                                                                                                                         <  2:336284/249‑1 (MQ=255)     agagCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGGGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGACGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGGGGTTTTAATTTGCA‑CAGCTTCCtgg                                                                                                                                                                                                                                       >  1:74869/1‑251 (MQ=255)                            tGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCGGGATTATTTTTGACCTGAGAGCCGGCATTATCGCCAAGCTAAGCATGCCGGGGCGTGATGGTGGTGGATTTGTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTAtt                                                                                                                                                                                                                   <  2:24600/248‑1 (MQ=255)                                   gATTCAGCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑gg                                                                                                                                                                                                        <  1:269809/251‑1 (MQ=255)                                                 aGGGCCTGACCATTGGAATTATTGCCTGGACTAGTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGAGTTTTCCTGGCCTGGATTCTCGGGATAGTCGGTGCGGTGGTGGGCGGCTGGCTGGCGACCATGTTTGGTATTGGCGGCGCCATCAGGGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGcctc                                                                                                                                                                                          <  2:257112/251‑1 (MQ=255)                                                 aGGGCCTGAACATGGAAATTATTGCCTGGATTATTTTTTACCTGAGAGCCGGAATTATCGCCAAGCTAATCATGCCGGGGGGTGATGGTGGTGGAGTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCGCGTTCT‑GGGCATATTCCGcctc                                                                                                                                                                                          <  2:270133/251‑1 (MQ=255)                                                      cTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGAATTTTTCATGACCTGTATTCCCGGGATCGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGAAACA‑CTTCCTAGGGGCGGTGGTGGGAGCTATTTTAGGTCT‑GGGGATATTCCGACTCCcgcg                                                                                                                                                                                     >  1:493180/1‑251 (MQ=255)                                                           cATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGGGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCggaggg                                                                                                                                                                                >  1:357144/1‑246 (MQ=255)                                                                     attGCCTGGATTATTTTTGGCCTGATAGCCGGCATTATCGCCAAGCTAAGCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGAtt                                                                                                                                                                       <  2:474841/250‑1 (MQ=255)                                                                                     ttGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAg                                                                                                                                                       <  1:205867/250‑1 (MQ=255)                                                                                       gACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCg                                                                                                                                                     <  2:110204/250‑1 (MQ=255)                                                                                        aCCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGAGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGGTTTAATTTGCA‑CAGCTTCCTGGTGGCGGGGGGGGGGGCTTTTCTCGTTCT‑GGGCATATTCCGCCCCCTGCGGAGGGGATAAGATTTTCATAAAGAGGATAGCGAt                                                                                                                                                   >  1:295525/1‑251 (MQ=255)                                                                                            gATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAg                                                                                                                                               <  1:343857/251‑1 (MQ=255)                                                                                                                     aTCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCCGTGGGTTTTATTTGCA‑CAGCTTCCTGGTGGCGGGGGGGGGGGCTATTTTCGTTTTTGGGC‑TATTCCGCCACCTGCGGAGGGGAAAAGATTTTCATAAGGCGGGTTGCGGTAACGATTCCGCCATCCGCTTT‑ctctatc                                                                                                                      >  2:386935/1‑244 (MQ=255)                                                                                                                                 cGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGGGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGGGAATAAGATTTTCATAAgg                                                                                                                                                              >  1:446258/1‑199 (MQ=255)                                                                                                                                 cGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGACTTTCATAAgg                                                                                                                                                              <  2:446258/199‑1 (MQ=255)                                                                                                                                  gTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGGGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATAACGATGCCGCTATCCGCTTT‑CACATTAGAACGTATACTCg                                                                                                         >  1:151966/1‑250 (MQ=255)                                                                                                                                        gtggtggATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTg                                                                                                  <  1:386935/251‑1 (MQ=255)                                                                                                                                                       tGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCCGTGGTTTTAATTTTCC‑CAGCTTCCTGGTGGCGGTGGTGGGGGATATTCTCGTTTT‑GGGCATATTCCGCCTCCTGCGGAGGGAAAAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTTTCCGCTTTTCACTTC‑GAACGTCTACTCGACCCCTGCTTTACggggggaa                                                                                    >  2:411635/1‑245 (MQ=255)                                                                                                                                                          ccTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCt                                                                                                <  1:318787/235‑1 (MQ=255)                                                                                                                                                             ttATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTGGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAAt                                                                               <  2:151966/248‑1 (MQ=255)                                                                                                                                                                        aTAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACtt                                                                  <  2:328545/251‑1 (MQ=255)                                                                                                                                                                                   ggtggtCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACTGGTGAAAAAAATCAATATAGCACTTCGATATCCGAt                                                       <  1:118930/251‑1 (MQ=255)                                                                                                                                                                                             ggctggctGGCGAACATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGATTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CCCATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGCTAAAGTCGGtt                                             >  1:138946/1‑251 (MQ=255)                                                                                                                                                                                                              gTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATTTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAacaacaaca                             <  2:357144/250‑1 (MQ=255)                                                                                                                                                                                                                                        gTTTTAATTTGCT‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCgtt  <  2:405922/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ACTTAGAGCGTTGATGTAAGCATTTCTTGCGTCCGATTCATCGAAACGAGGGCCTGAACATGGGAATTATTGCCTGGATTATTTTTGACCTGATAGCCGGCATTATCGCCAAGCTAATCATGCCGGGGCGTGATGGTGGTGGATTTTTCCTGACCTGTATTCTCGGGATAGTCGGTGCGGTGGTCGGCGGCTGGCTGGCGACCATGTTTGGCATTGGCGGCTCCATCAGTGGTTTTAATCTGCA‑CAGCTTCCTGGTGGCGGTGGTGGGAGCTATTCTCGTTCT‑GGGCATATTCCGCCTCCTGCGAAGAGAATAAGATTTTCATAAGGCGGATAGCGATACAGATGCCGCTATCCGCTTT‑CACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTT  >  NC_000913/1244669‑1245150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑