Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,227,656 G→A intergenic (‑184/‑25) pliG ← / → ycgL inhibitor of g‑type lysozyme/PF05166 family protein YcgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,227,6560GA96.7% 87.1 / NA 30intergenic (‑184/‑25)pliG/ycgLinhibitor of g‑type lysozyme/PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (13/16);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGG‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGG  >  NC_000913/1227410‑1227900
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
aaaGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑cgcg                                                                                                                                                                                                                                                  >  1:267813/1‑251 (MQ=255)
    cTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGtt                                                                                                                                                                                                                                              >  1:7571/1‑251 (MQ=255)
        ggtaGGTTGGCAGTGCAAGCAGAAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCTTTTTTGCCGGTGGGATTACTTATAAAGGTGGCATGGACTCAACAAACCGTAGAAATCTACAGCTTGCTGGCTTGTGA‑CGCGGGTTTc                                                                                                                                                                                                                                            <  2:102136/246‑1 (MQ=255)
                   aGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCt                                                                                                                                                                                                                               >  1:150876/1‑251 (MQ=255)
                          gcagcaATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGcc                                                                                                                                                                                                                         >  1:315032/1‑250 (MQ=255)
                                   cAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGc                                                                                                                                                                                                               <  2:7571/251‑1 (MQ=255)
                                             gATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTTGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAag                                                                                                                                                                                                      <  1:497999/250‑1 (MQ=255)
                                                                 gTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTtgtgtg                                                                                                                                                                                 >  2:2070/1‑251 (MQ=255)
                                                                   aggctcCTGTTCAGAATGATTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGTATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGa                                                                                                                                                                                <  2:174637/247‑1 (MQ=255)
                                                                   aGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGAt                                                                                                                                                                               >  1:96183/1‑251 (MQ=255)
                                                                        ccTGTTCAGAATGAGTACTATGAACTGATATAATATAACAATCCCGAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGTACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAATCCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGAttt                                                                                                                                                                             <  1:441126/248‑1 (MQ=255)
                                                                           ggtCAGAATGAGTACTATGAACTGTTATAATTAAACAATCCCTAACGGGAGAATTCCAGTGGCTTTTCTGGGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑AGCGGGTTTCATTGATGTTAGCGTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCg                                                                                                                                                                         <  2:353924/247‑1 (MQ=255)
                                                                                         cGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGAc                                                                                                                                                         <  1:355100/251‑1 (MQ=255)
                                                                                                        atataaCAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGTTAAAATCACGTTGCGACATTTTTGCCGGGGGGCTTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCAACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGAGGTTAGCTTATACCGAAACCTGGCATCTTAAGGAGTAAAAGAATGTTTTGTGTGATTTATAGAAACAGCACAAGTGACCAGACCGATTTatat                                                                                                                                          >  1:406020/1‑251 (MQ=255)
                                                                                                                aTCCCTAACGGGAGAATACCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATGTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGaaaa                                                                                                                                  <  2:150876/251‑1 (MQ=255)
                                                                                                                      aaCGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAACGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAg                                                                                                                                                               >  2:371466/1‑216 (MQ=255)
                                                                                                                      aaCGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAg                                                                                                                                                               <  1:371466/216‑1 (MQ=255)
                                                                                                                                                   ttCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACtg                                                                                                >  1:498862/1‑250 (MQ=255)
                                                                                                                                                                   tGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTACAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGCAAGGTTTTGGTc                                                                                >  2:192554/1‑250 (MQ=255)
                                                                                                                                                                      ggCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCTACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGcc                                                                            >  2:160055/1‑251 (MQ=255)
                                                                                                                                                                        cctttttGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGCAATCTACAGCTGGCTGGCGTGTGA‑CGCGGGATTCATTGATGTTAGCTTATGCCAAAAACTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAACAAAAGACGATTTTTCGCGTGTTCCTTAGGAACTGATGAAAGGTTTTGGTCAGCCTc                                                                          >  2:405490/3‑251 (MQ=255)
                                                                                                                                                                                                             gACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaagaa                                     <  1:192554/251‑1 (MQ=255)
                                                                                                                                                                                                                   acaaACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCTGCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGt                               <  1:286679/251‑1 (MQ=255)
                                                                                                                                                                                                                   acaaACCGTAGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGt                               <  1:307019/251‑1 (MQ=255)
                                                                                                                                                                                                                     aaaCCGTAGAAATCTACAGCTGGCTGGTTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTAGATGTCGAAAAAAAAGACTATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGTTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCa                             <  1:220250/251‑1 (MQ=255)
                                                                                                                                                                                                                       acctgaGAAATCTACAGCTGGCTGGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAAt                           <  2:232199/246‑1 (MQ=255)
                                                                                                                                                                                                                            aGAAATCTACAGCTGGCTGTCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTAGCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTGCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCg                       <  2:188966/250‑1 (MQ=255)
                                                                                                                                                                                                                             gAAATCGACAGCTGGCTTGCTTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATGTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTGCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGat                     <  2:417214/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aCAGCTGGCTGGCTT‑TGGACGCGGGTTTCATTGATGTTAGCTTATGCCAAATCCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGGGATTTATCGAAGCAGCAAGCGTTACCAGGCCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATAtt                  <  1:15537/247‑1 (MQ=255)
                                                                                                                                                                                                                                                ctTGTGA‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAgg  >  1:308641/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     
AAAGCTCGTGGAGGTTAGCAGTGCAAGCAGCAATACAGCCTTCCTGATGCTCTTGATTTTCATTGGTAGTCTCCTGTTCAGAATGAGTACGATGAACTGTTATAATATAACAATCCCTAACGGGAGAATTCCAGTGGCTTTCCTGCGTTCGGTAAAATCACGTTGCGGCATTTTTGCCGGGGGGATTACTTATAAAGGGGGCATGGACTCAACAAACCGTAGAAATCTACAGCTGGCTGGCTTGTGG‑CGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGG  >  NC_000913/1227410‑1227900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑