Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,201,550	C→A	T18P (ACC→CCA)	ymfI →	uncharacterized protein YmfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,201,550	0	C	A	97.1%	106.0 / NA	34	T18P (ACC→CCA)	ymfI	uncharacterized protein YmfI
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (13/20);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCT  >  NC_000913/1201317‑1201776                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   tATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAAtt                                                                                                                                                                                                                   >  1:291196/1‑251 (MQ=255)           aGGTCGATGGTCAGCCTCAACCTTAACCCGCGTGCGCTTAGCCGCATGCACCCCAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGGTATGTGCCTGTCTTTTCACCACTTCAGGCTCAGTGGTATGCTGGAGTACTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAAc                                                                                                                                                                                                         <  1:309529/251‑1 (MQ=255)                gATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCt                                                                                                                                                                                                    <  1:35562/251‑1 (MQ=255)                               ccTTAACCCGCGTGCGCTGAACCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGt                                                                                                                                                                                     <  2:271299/251‑1 (MQ=255)                                cTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGtt                                                                                                                                                                                    >  2:192567/1‑251 (MQ=255)                                       cgcgTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCTCCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCCAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTaaa                                                                                                                                                                                 >  2:55692/1‑247 (MQ=255)                                       cgcgTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCTCCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTaaa                                                                                                                                                                                 <  1:55692/247‑1 (MQ=255)                                          gTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTAATTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAACAAGTTaaaaaatctg                                                                                                                                                                          >  2:320717/1‑244 (MQ=255)                                                  aGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAgc                                                                                                                                                                  <  1:402848/251‑1 (MQ=255)                                                   gCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAgct                                                                                                                                                                 <  1:192567/251‑1 (MQ=255)                                                               acaACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTAATTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGa                                                                                                                                                     <  1:320717/251‑1 (MQ=255)                                                                   cGCTAAGGATTCTCTCTGGTTGAACATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGCCGATAGCTGCTGCAGACTGATTcc                                                                                                                                                 >  2:307691/1‑251 (MQ=255)                                                                                              aCTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAAt                                                                                                                      <  1:505537/251‑1 (MQ=255)                                                                                                  aGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGaa                                                                                                                   <  1:210919/250‑1 (MQ=255)                                                                                                               cTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATcc                                                                                                     >  1:94780/1‑251 (MQ=255)                                                                                                                   catgtCACCCCTGCAGGCCCGGTGGTATGCTGGAGTTCTCACACATCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCCCGTTCGTCTATTTGAAGACACAGTCGAACATCCAAAAAAATTTGAAGAACTAATTCGTAACTTTCTGTACCATAAAACAGTTAAAGAGTCTGACCATAGCTGCTGCAGACTGATTCCATAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGa                                                                                                 <  1:39812/247‑1 (MQ=255)                                                                                                                    ttttCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGAATGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGAt                                                                                                >  2:226724/1‑251 (MQ=255)                                                                                                                           cacTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACAt                                                                                         >  1:124307/1‑251 (MQ=255)                                                                                                                                cAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTc                                                                                     >  2:392700/1‑250 (MQ=255)                                                                                                                                      cgtgggtATGCGGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTg                                                                                <  2:360324/245‑1 (MQ=255)                                                                                                                                              tcctggggatctgacacaGCCAGCAAACAAGGACACTTAATGAACCAGTTTTATGTTAACGTTCGTCTAATTGAAGACACCGCCGAAAAGCCAAAAAAATTTGAAGAATTAAGACTTAACTTTTTGTACCAACAAACAGTTAAAGAGTCTGACGATAGCTGCTGCTGATCGATTCCAGAGGGGTACATACTCAACAGAACAAGGAACTGCCACCAAATCCTTGATCAAACATTTTCAATTACTacccgtgc                                                                      >  2:412017/14‑244 (MQ=255)                                                                                                                                               gCTGGAGATCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGAGCAAACATTTTCAATTGCTAACAGTGcc                                                                     >  2:343347/1‑251 (MQ=255)                                                                                                                                                               agccagcAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAata                                                     <  1:117746/251‑1 (MQ=255)                                                                                                                                                                   agcaagcaAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATAtt                                                 <  1:71004/251‑1 (MQ=255)                                                                                                                                                                       agcaagGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCTAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTTATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGAGCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTc                                             <  1:288200/251‑1 (MQ=255)                                                                                                                                                                        gcaagGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGATTTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCa                                                         >  1:395056/1‑238 (MQ=255)                                                                                                                                                                        gcaagGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACGTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATATCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATGTTCAATTGCTAACAGTGCCGGTGTTGACGCa                                                         <  2:395056/238‑1 (MQ=255)                                                                                                                                                                                    aaTGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGTAAATATATTTGTCTGTAAATTTGaa                                 <  1:361297/250‑1 (MQ=255)                                                                                                                                                                                        aaCCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGTACaa                              >  2:154716/1‑249 (MQ=255)                                                                                                                                                                                             gTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCAt                       <  2:291196/251‑1 (MQ=255)                                                                                                                                                                                               tttATGTTCACGTGCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGAGAGCGGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAAGTCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATGTGGCTGTAAATTTGAACAAAGCGCagg                      <  2:200332/250‑3 (MQ=255)                                                                                                                                                                                                    gTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTAc                 <  1:88581/250‑1 (MQ=255)                                                                                                                                                                                                                  aTTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCtt     <  1:434032/248‑1 (MQ=255)                                                                                                                                                                                                                  aTTTGAAGACACAGCCGAACAGCCAAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCt  >  1:386415/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   TATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAAAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCT  >  NC_000913/1201317‑1201776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑