| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,193,141 | G→A | G211G (GGC→GGT) | mnmA ← | tRNA‑specific 2‑thiouridylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,193,141 | 0 | G | A | 96.9% | 87.5 / NA | 32 | G211G (GGC→GGT) | mnmA | tRNA‑specific 2‑thiouridylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (16/15); minor base C (1/0); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCCGACGTACG > NC_000913/1192899‑1193387 | aaCTGCTGGGCAATCAACCCGACAGTCATCAGCCGCGGGTGTTCAGGGCCCTGAGCGACAATCAGAATGT‑GTTTGTCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCGTCTTGGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGGAATGATTTGGCCCGGTTGCGCCGGGAGATAACggtgaagtgt < 2:212146/251‑9 (MQ=255)aaCTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTAGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCCTCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGAAAACGCCCCAGGa > 2:300618/1‑250 (MQ=255) gtgGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTcgc < 1:463741/250‑1 (MQ=255) gggCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCgg < 2:325349/251‑1 (MQ=255) agacaTCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCaa < 1:475660/251‑1 (MQ=255) cAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAg < 1:65802/250‑1 (MQ=255) ccGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCaaa < 2:118469/251‑1 (MQ=255) agggccctgctggACAACCAGAAGGTTTTTT‑TCGCCGTCTTTTCCCACCACATACCAAGGTGCTTCGGTACATTCGTTGGGGCCACCGATACCCAGAACGTTAGGCTGACCGAGAGTGTGAGACATCAGCCCCTGGTGCT‑ACCAAATGTCATCGCCATCGACGGTAATGATTTTGCCCGGTAGCGCCGGGAGATAACGACACAGGAAATCGCTGAATTTACTTTCGCCAATGAAGCAAATGCCGGTagcgc < 2:123999/239‑4 (MQ=255) ccTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTACACCACATACCACGGTTCTTCGGTACTTTCTTTGGTGCCACCGATACCCAGATCTTTACGTTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATTGCCATCGACGGTAATGATTTTGACCGGTTGCGCCGGGAGATCACGACCCAGGAACTCGCGGTATTTACGTTCGCCATTGAAGAAAATACCGGTAGAGTAtttt > 2:444025/1‑251 (MQ=255) gACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGATACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGACTTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTcg > 2:504861/1‑251 (MQ=255) gAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTTGAGTCTTTTTTCTTCGCGGTgacc > 2:166330/1‑251 (MQ=255) gAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTgacc > 1:230706/1‑251 (MQ=255) tCGACGTCTTTGGCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGATGACCGAGAGTGTGATACATCAGCCACTGGTGCTCACCAC‑TTTCATCGCCAACGACGGTAATGATTTTGCCCGGGTGCGCCGGGAGCTAACGACCCAGGACATAGCGGATTTTACGTTCGCCAATGAAGCAAAAGCAGGAAGCGTCTTTTTTCTTCGCGTTAGCCAGACCAAGacc > 2:303507/1‑249 (MQ=255) acaTACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGATCACCAA‑TTTCATCGCCATCGACGGTACTGCTTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGCACTCGCGGAATTTACGTTCGCCAATGAAGCAAATACCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCCATCTTACGCAc > 1:254448/1‑251 (MQ=255) ggTTCTTCGGGACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGCCCAGAACAAGGTCTTCAGCAATCTTACGCCCATGCgggtt > 2:316326/1‑248 (MQ=255) tcGGTACCTTCTGTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCa < 1:241243/250‑1 (MQ=255) tcttTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGAc > 1:505240/1‑251 (MQ=255) aCCCAGACCTGTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGCCGGTAATGATTTTGCCCGGTTGCGCCGGGTGATAACGACCCAGGAACTCGCGGAATTTCGGTTGGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGTCGAACAGACCAAGCTCTTCAGCACTATTCAGCACCTGCGGGTTTTCCAGTTCGCCGACCGGGAACAGTCTTTg > 2:76002/1‑249 (MQ=255) ttACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGGAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCGTACGCACCGGCGGTTGTTCCAGTGCGCCGACCGGGAACAGGCTTTGCGCAATCTGc < 2:435566/249‑1 (MQ=255) aGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGct < 1:490211/251‑1 (MQ=255) cccTGGTGCTCACCAA‑TTTCATCGCCATCGCCGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGACGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTg > 1:317482/1‑251 (MQ=255) ggTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGt < 1:504861/249‑1 (MQ=255) ccAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCt > 1:386896/1‑251 (MQ=255) tCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGTATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCCGTTCGCCAACCGGGAACAGGCTTTGCGCAATCTGATCATGGCTGAGCGTATACAGGAAGTAGCTCTGGTCTTTTTTGCTGTCCAGACCACgc > 2:442325/1‑249 (MQ=255) ggTAATGATTGTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAggc < 1:481204/251‑1 (MQ=255) gTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGCAGTAGCTCTGGTCTTTATTGCTGTCCAGCCCAc > 2:220232/1‑241 (MQ=255) gTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCAc < 1:220232/241‑1 (MQ=255) tAATGATTTTGCCCGGTTGCGCCGGGAGATAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAggcgg > 1:37174/1‑251 (MQ=255) gTTGCGCCGGGATATAACGACCCAGGAACACGCGGAACTTATGTTCGACAATGAAGCAAATGCCGGTAGATTCTTTTTTCTTCGCGTTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCATGTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCACATGGCTGAGCTTATAAAGGAAGTAGCTCTGGTCTTTATGGCTGTCCAGCCCACGCAGCAGGCGGCTCTTGCCATCGACa < 2:172076/251‑1 (MQ=255) gcCGGGAGATAACGACCCAGGAACTCGCTGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGACATAGCTCTGGTCTTTATTGCTGTCCAGACCCCGCAGCAGGCGGCTCTTGCCATCGCCATCGGc > 1:241348/1‑251 (MQ=255) gagaTAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCCGac < 2:386896/251‑1 (MQ=255) agaTAACGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCCGacg < 1:383744/251‑1 (MQ=255) aaCGACCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGGCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCCGacgtacg > 2:385501/1‑251 (MQ=255) | AACTGCTGGGCAATCAACCCGACAGACATCAGCCGCGGGTGTTCATGGCCCTGAGCGACAACCAGAATGTTGTTT‑TCGACGTCTTTGTCCACCACATACCACGGTTCTTCGGTACCTTCTTTGGTGCCACCGATACCCAGACCTTTACGCTGACCGAGAGTGTGATACATCAGCCCCTGGTGCTCACCAA‑TTTCATCGCCATCGACGGTAATGATTTTGCCCGGTTGCGCCGGGAGATAACGGCCCAGGAACTCGCGGAATTTACGTTCGCCAATGAAGCAAATGCCGGTAGAGTCTTTTTTCTTCGCGGTGACCAGACCAAGATCTTCAGCAATCTTACGCACCTGCGGTTTTTCCAGTTCGCCGACCGGGAACAGGCTTTGCGCAATCTGCTCATGGCTGAGCGTATAAAGGAAGTAGCTCTGGTCTTTATTGCTGTCCAGACCACGCAGCAGGCGGCTCTTGCCATCGACATCGGCCCGACGTACG > NC_000913/1192899‑1193387 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |