Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,180,126	G→A	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB → cobB →	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,180,126	0	G	A	95.7%	130.9 / NA	48	A203T (GCG→ACG)  A203T (GCG→ACG)	cobB cobB	protein‑lysine deacetylase/desuccinylase/lipoamidase protein‑lysine deacetylase/desuccinylase/lipoamidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (23/21);  minor base T (0/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCT  >  NC_000913/1179879‑1180357                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        cGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGt                                                                                                                                                                                                                                      <  1:179403/251‑1 (MQ=255)           tttttGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCAGCACGTAGTGAGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACTGTGTCGATGGc                                                                                                                                                                                                                            >  2:357601/1‑251 (MQ=255)                 cTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGatat                                                                                                                                                                                                                      >  1:168303/1‑251 (MQ=255)                   ggTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGGCGATGGCCGATAttt                                                                                                                                                                                                                    <  1:113347/251‑1 (MQ=255)                      gactcAGAATATTGACCACCTGCATGGTCGCGTAGGTAATCCCAATGTGATTCATATGGATGGGTAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCTCTCTGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCa                                                                                                                                                                                                                 <  2:99662/247‑1 (MQ=255)                            gAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGATGTCGATGGCCGATATTTTCATTGcc                                                                                                                                                                                                            >  1:218067/1‑250 (MQ=255)                                        ccTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCg                                                                                                                                                                                               >  1:361518/1‑251 (MQ=255)                                                aaCGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGtt                                                                                                                                                                                       <  2:114262/251‑1 (MQ=255)                                                               ccAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTggg                                                                                                                                                                        >  2:505308/1‑251 (MQ=255)                                                                      gATTCATAGGCATGGGGAACTGCTGAAAGTGCTTTGTTCACAAAGAGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTc                                                                                                                                                                 <  2:491282/251‑1 (MQ=255)                                                                          cATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACg                                                                                                                                                              <  1:442882/250‑1 (MQ=255)                                                                                 aTGGGGAACTGCTGCAAGTGCGTTGTTCACAAAGTGGTCAGGATCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCCCATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGACGCGaa                                                                                                                                                       >  2:231970/1‑250 (MQ=255)                                                                                 aTGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGGCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGaaa                                                                                                                                                      <  1:454295/251‑1 (MQ=255)                                                                                  aggtgAACTGCGGAAAGAGCGTTGTTCACAAAGTGGTCGGGGTCTCGACTGGACAGGTGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTTCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCCCTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCCTTGCCATTGGCACATCCGGGCAGGTTTATCCGGCGGCTGGTTTTGTTCACGAAGCGaa                                                                                                                                                       <  2:133450/245‑1 (MQ=255)                                                                                     ggAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGc                                                                                                                                                  <  2:126078/251‑1 (MQ=255)                                                                                      gAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCTCGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCa                                                                                                                                                 <  1:233911/251‑1 (MQ=255)                                                                                       aaCTGCTGAAAGTGCGTTTTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCAt                                                                                                                                                <  1:226587/251‑1 (MQ=255)                                                                                               aaaGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGAACAGGAGACGTTAAACCAGAAGCTAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCACGCACGTAGTGTGGTTTGGCGAAATGCCAATCGGCATGGATGAAATTTAAATGACATTGTCGATGGCCGATATTTTCATTTACATTGGCACATACGGGCATGTTTATCAGACGGCTGGGTTTGGTCCAGAAGCGAAACTGCAAGgctcac                                                                                                                                         >  1:435832/1‑246 (MQ=255)                                                                                                aaGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGCTAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGCTGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGcac                                                                                                                                       >  2:424898/1‑251 (MQ=255)                                                                                                aaGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGcac                                                                                                                                       >  2:14103/1‑251 (MQ=255)                                                                                                                 aGTGGTTAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTTCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGTCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCt                                                                                                                      <  1:296745/251‑1 (MQ=255)                                                                                                                    ggTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGGGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGa                                                                                                                   <  2:358782/251‑1 (MQ=255)                                                                                                                     gTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGa                                                                                                                   >  1:142904/1‑250 (MQ=255)                                                                                                                        aGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCg                                                                                                               >  1:377765/1‑251 (MQ=255)                                                                                                                               cGACTGGACAGGAGACGTTACCCCAGAAGATAAAGGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATAGGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCa                                                                                                          <  2:438618/249‑1 (MQ=255)                                                                                                                                    ggACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTTTTGTTTGGCGAAATGCCACTCGGCATGGATTAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTTGCACATCCGGTCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTg                                                                                                     <  2:198199/249‑1 (MQ=255)                                                                                                                                         ggAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATg                                                                                               >  1:374141/1‑250 (MQ=255)                                                                                                                                             aCGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGAGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGACATTGGCACATCCGGGCATGGTTATCCGGCGGCTGGGTTTGGTAACGCAGCGAAACTGCCTGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGCAttt                                                                                          >  2:432827/1‑251 (MQ=255)                                                                                                                                               gTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGc                                                                                        >  1:416851/1‑251 (MQ=255)                                                                                                                                                   ccccAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGACGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGGGTCAGGTTGGTAATGAATTTGCCg                                                                                      >  1:469505/1‑249 (MQ=255)                                                                                                                                                          agaTAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATAtt                                                                             <  1:360927/251‑1 (MQ=255)                                                                                                                                                             tAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACg                                                                          >  1:441301/1‑251 (MQ=255)                                                                                                                                                                    cATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGGGAAATATTACGGCCCGGc                                                                   >  2:122729/1‑251 (MQ=255)                                                                                                                                                                     aTTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGACACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGc                                                                   >  2:403782/1‑250 (MQ=255)                                                                                                                                                                       tgttgCCAGGTTCCGGCACCCTTGCGCCCGCACGTAGTGTGTTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCaa                                                                 <  2:192247/250‑1 (MQ=255)                                                                                                                                                                        gttgCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGc                                                               <  1:444516/251‑1 (MQ=255)                                                                                                                                                                           gCCAGTTTCCGGCCCCCTTGCGCCCGCGCGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACTTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCa                                                             <  2:307814/250‑1 (MQ=255)                                                                                                                                                                                                 cccGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGaa                                       >  2:256332/1‑250 (MQ=255)                                                                                                                                                                                                  ccGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGAATGGAGTAAATTTATATGACGTTGTCGATGGCCGTTATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGaaaa                                     <  2:361518/251‑1 (MQ=255)                                                                                                                                                                                                      ccGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTg                                 <  1:336614/250‑1 (MQ=255)                                                                                                                                                                                                                   gagagAAAGGCCACTCGGAATGGATGAAATTTATATGCCGTGGTCGATGGCCGATATTTTGATTGCCTTTGGCAGATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAAGGAATTTGCCGAGAAATATTACGGCCCGGCAAGACAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTa                     <  2:86917/246‑1 (MQ=255)                                                                                                                                                                                                                     gcaaaaggtctcTCGGCAGGGATGAAGTTTATATGGCGTTGTTGATGGCCGATATTGTCATTGCAATGTGAACATCCGGGCATGGTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGAGTaaaa                  <  2:84102/240‑1 (MQ=255)                                                                                                                                                                                                                     gCGAAATGCCACTGGGCCTGGATGAAATTTATATGTCGTTGGTGATTGCCGATATTTTCATGGCCATTGGCACATCCGGGCAGGGTTATCCGTCCTCTGGGTTTGTTCATGAAGGTAAACTGCATTCCGCCCACCCCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTAGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTaaaa                  <  1:506730/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAATGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGc                <  1:14103/251‑1 (MQ=255)                                                                                                                                                                                                                        aaaTGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAATTTGCTGAAGGGATTAAAAGCg               >  1:117883/1‑251 (MQ=255)                                                                                                                                                                                                                          aTGCCACTCGGCATGGATGACATTTCTATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCCCACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGCGAAATATTACGGCCCGGCCAACCCGGGGGGGCCTGAGTTTGTTTGAAAGTTGCTGAAGGGGTTAAAAGCggg             >  2:378382/1‑251 (MQ=255)                                                                                                                                                                                                                          aTGCCACTCGGCATGGATGAAATTTATATGACGTTGTCGATGGCCGACATTCTCATTGACATTGGCACATCCGGGCATGTATATCAGGCAGCTGGGGTTGTACACGAAGCGAAACTGCATGACCCGCACACAGTGGACCTGAATCTTGACCCGAGTCAGGTTGGTAATGAATTTGCCGAGTAATATTACGGCCAGACATGCCAGGGAGTACCTCAGTATGTTGCAAAGTAACTGAAGGGATTACAAACggg             >  1:215672/1‑251 (MQ=255)                                                                                                                                                                                                                                     cATGGATGAAATTTATATGACGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGACAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCt  >  1:88585/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                        CGGCGATCGCTTTTTGCTGGTGACGCAGAATATCGACAACCTGCATGAACGCGCAGGTAATACCAATGTGATTCATATGCATGGGGAACTGCTGAAAGTGCGTTGTTCACAAAGTGGTCAGGTTCTCGACTGGACAGGAGACGTTACCCCAGAAGATAAATGCCATTGTTGCCAGTTTCCGGCACCCTTGCGCCCGCACGTAGTGTGGTTTGGCGAAATGCCACTCGGCATGGATGAAATTTATATGGCGTTGTCGATGGCCGATATTTTCATTGCCATTGGCACATCCGGGCATGTTTATCCGGCGGCTGGGTTTGTTCACGAAGCGAAACTGCATGGCGCGCACACCGTGGAACTGAATCTTGAACCGAGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCT  >  NC_000913/1179879‑1180357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑