Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,161,403	G→A	A50V (GCC→GTC)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,161,403	0	G	A	97.0%	97.2 / NA	34	A50V (GCC→GTC)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/19);  minor base C (1/0);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAA  >  NC_000913/1161162‑1161648                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ataGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCt                                                                                                                                                                                                                                              <  1:398475/251‑1 (MQ=255)              tGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGt                                                                                                                                                                                                                                 >  1:429873/1‑251 (MQ=255)                gAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCCCCGttt                                                                                                                                                                                                                               >  2:60110/1‑251 (MQ=255)                        gggAATAATAAAGAGCACGTTCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGAAGACGTTACGCTGTAATCATTTTCGCCATCCTCTGGAGCTGTGCCTGAACCCTCAACAATCACCGTTTCTTCattg                                                                                                                                                                                                                       >  2:48945/1‑248 (MQ=255)                             taataaAAAGCCCGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTCGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGCACCCGCAGAGGGAGACGTCACGCTGTAATCATTTTCCCCATCATCTGGAGCTGGGACTGAACCCACAACAATCAACGTTTCTTCAGTGGCTgg                                                                                                                                                                                                                  >  2:94958/1‑251 (MQ=255)                              aataaAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGt                                                                                                                                                                                                                 >  1:61733/1‑251 (MQ=255)                                             cGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCAGCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCa                                                                                                                                                                                                  <  1:48945/251‑1 (MQ=255)                                              ggAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGc                                                                                                                                                                                                   >  1:221389/1‑249 (MQ=255)                                                 aTCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGa                                                                                                                                                                                                >  1:135730/1‑249 (MQ=255)                                                                     tCCCCAGCGTGTTTTACATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATAACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGACGCAACAGCGGCAGCAGGTAAAAGTggcaatggtatg                                                                                                                                                                          >  1:506976/1‑240 (MQ=255)                                                                         cAGCGTGTTTTCCATCTCTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGGTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAAc                                                                                                                                                                      <  2:142320/251‑1 (MQ=255)                                                                                      acctctccgcccacaggttttAACTGCTGATCTGCCATCCGCTGCGTTCTAACAATAGTGACCGACTGAGTAATATCTCGTTGAGTCATCGGTATTTTTGTACCCTCAGAGTTAGACGTCTCTCGGTAATCTTTTGTTACATCTTCTGGAGATGTGACATAACACACAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAATTCGGCATTAGGTAATTTTGCCAGTCCTATGCAACCGGCAAGGAGTGa                                                                                                                                                         <  2:446326/231‑1 (MQ=255)                                                                                       tCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGGGCTATGCAACCGGCAAGTAGTGAc                                                                                                                                                        >  2:136415/1‑251 (MQ=255)                                                                                                 cAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGa                                                                                                                                              <  1:242008/251‑1 (MQ=255)                                                                                                      ctgagaactgctgtccctCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCCCGTTGAGTCATCTGCATGTTGGTACCCGCAGATGTAGACAGTACACTGTAATCATTTTCGACATCATCTGGAACTGTGACTGAACCCCCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAAGAGGGCCAGTGCTATGCAACCGGTAAGTAGTGACGTTTTGGTGATg                                                                                                                                            <  2:204185/231‑1 (MQ=255)                                                                                                       ttggaaCTGCTGATCTTCCAGCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATGTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCGTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCt                                                                                                                                         <  2:221389/246‑1 (MQ=255)                                                                                                         gTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGa                                                                                                                                      <  1:282411/251‑1 (MQ=255)                                                                                                          tAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGat                                                                                                                                     >  1:238458/1‑251 (MQ=255)                                                                                                               gctgATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATAtt                                                                                                                                  <  1:24714/249‑1 (MQ=255)                                                                                                                       tCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATTCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTa                                                                                                                             <  1:382496/246‑1 (MQ=255)                                                                                                                                        aaCAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGaa                                                                                                        <  1:60110/250‑1 (MQ=255)                                                                                                                                              aGTGCCCGACTGAGGAATATCTCGTTGAGTCAGCTGAATTTTGGTACCCGCAGAGGGAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCTTTTCTTCAGTGGCTGGTTCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCGATGCAACCGGCAAGTAGTGACGGTAGGGTTATGTCTGGATATTGAATATCCCTGTTAAATTGTGTTGAAAGCAttt                                                                                                 <  1:438736/251‑1 (MQ=255)                                                                                                                                              aGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGGACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCAttt                                                                                                 <  2:201504/251‑1 (MQ=255)                                                                                                                                                  cccGACTGAGGAATATCACGTTTAGTCATCTGCATTTTGGTACTCGCAGAGGTTGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGGTAAATTGTGTTGAAAGCATTTGaac                                                                                             <  2:366703/250‑2 (MQ=255)                                                                                                                                                  aCCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTTGTACCCGCAGAGGTAGACGTTACGCTTTAATCATTTTCGCCATCATCTGGAGCGGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGaaa                                                                                             <  2:392974/251‑1 (MQ=255)                                                                                                                                                         gAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCtt                                                                                       <  2:61733/250‑1 (MQ=255)                                                                                                                                                                                  cATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGc                                                             <  2:182710/251‑1 (MQ=255)                                                                                                                                                                                              cGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGtg                                                 >  1:330338/1‑251 (MQ=255)                                                                                                                                                                                                     gTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCg                                          <  2:301449/251‑1 (MQ=255)                                                                                                                                                                                                                        cATTTTCGCCATCATCTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGCGGGTGATGAGCGATTATATTTAGTGTGCGa                        >  1:45242/1‑250 (MQ=255)                                                                                                                                                                                                                                    catcTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATtttgtttg           <  1:405474/251‑1 (MQ=255)                                                                                                                                                                                                                                     atcTGGAGCTGTGACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGGGCCAGTGCTATGCAACCGGCAAGTAGTGGCGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGGAATGAGCGATTATATTTAGTGTGCGAATAATTttgtttgt          >  1:434409/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccggaacTGTGTCTGAACCCCCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAATGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATTGCTTGATATTGATTATCCCTGTTAAATTGTGTGGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATTCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGGTTGTaa        <  2:108128/245‑1 (MQ=255)                                                                                                                                                                                                                                              tgtgACTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCaa  >  1:153235/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ATAGTTATCGATCTGGAATCCGCGGGAATAATAAAGAGCACGATCGGAATCCGCCTGACTTTTGCTGATCCCCAGCGTGTTTTCCATCACTTCGCCCAGCGTTTGTAACTGCTGATCTTCCATCCGCTGCTGGCTAACAATAGTGACCGACTGAGGAATATCACGTTGAGTCATCTGCATTTTGGTACCCGCAGAGGTAGACGTTACGCTGTAATCATTTTCGCCATCATCTGGAGCTGTGGCTGAACCCTCAACAATCACCGTTTCTTCAGTGGCTGGTGCAGCAAAAGCGGCAGAAGGTAATAGTGCCAGTGCTATGCAACCGGCAAGTAGTGACGGTTTGGTGATGGCTTGATATTGATTATCCCTGTTAAATTGTGTTGAAAGCATTTGAAATCTCTTGGTTTATGTTGTCGTCAGGCTCATGCCGGAGGAGGGTGATGAGCGATTATATTTAGTGTGCGAATAATTTTGTTTGTAAATGCAA  >  NC_000913/1161162‑1161648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑