Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,110	C→T	E162E (GAG→GAA)	yceF ←	m(7)GTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,110	0	C	T	95.5%	122.7 / ‑4.0	44	E162E (GAG→GAA)	yceF	m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (18/24);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

CGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAAT‑ACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTT  >  NC_000913/1145867‑1146355                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ggttttttGCAAAAGCTGCATAACGCGC‑TTAATTACAAGCATATAAACCGTATAATGGGTGGTTGGCCGGATAAGGCGTTTACGCCGCATCTGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACa                                                                                                                                                                                                                                                <  2:166748/246‑1 (MQ=255)  gttgttTGCAAAAGCTGCGTAACGCGCGCTAAT‑ACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAAc                                                                                                                                                                                                                                                 >  1:116404/1‑249 (MQ=255)  gttgtgTGCAAAAGTGGCGTAACGCGTGCTAAT‑CCAAGCCTATAAACCTGATAATGGGCGGTTGGCCTGATATGGTGTTTACGCCGCATCAGGCCGCCAGTCCCGATTGCCGGATGCGACGTAACCACATCAGGCGCAAAAAACTACCCTATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAg                                                                                                                                                                                                                                               <  1:469788/251‑1 (MQ=255)                     tAACGCGCGCTAAT‑ACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCCCGATTAGCGGTAAACCAACCAGCGTGTTAGGAACACGCCCCTCTAAACTTTCAAACAGCGTAATGCCAAATCCTTcc                                                                                                                                                                                                                            >  2:171040/1‑250 (MQ=255)                      aaCGCGCGCTAAT‑ACAATCATATAAACCTGATAATGGGCGGTTGTCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCa                                                                                                                                                                                                                            <  1:282290/250‑1 (MQ=255)                                      aaGCATATAAACCTGATAATGGGCGGTTGGCCGGATAAGGGTTTTAACCCGCATCAGGCCGCCAGCACCGGTTGCCGGATGCGACGTAACCACAGCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGTCGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCcg                                                                                                                                                                                                            <  2:304595/251‑1 (MQ=255)                                            ataAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAgg                                                                                                                                                                                                      >  1:14872/1‑250 (MQ=255)                                                    ggaaaTGGGCGGTGGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGAGTGCCGGATGCGATGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCGTCACTCTTAAAGCTACCCGCGCAGTGCAggg                                                                                                                                                                                                <  2:173949/246‑1 (MQ=255)                                                       aaggggCGGTTGGCCTGATAAGGCGTTTCCCCCCCAACGGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCCCATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATg                                                                                                                                                                                            <  2:283832/247‑1 (MQ=255)                                                           ggCGTTTGTCCTCATAATGCTTTTTCGCCGCATAAGTCCACCATCACCGATTGCCTGATGCGACATAACCACATCCAGCACAAAAAACTAACCCATCAACAGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCATCGTTTTAGGATCACTCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCACTTCAGGGGATACTCtt                                                                                                                                                                                       <  2:221023/251‑1 (MQ=255)                                                           ggCGGTTGGCTTGATAAGTCGTTTACTCCGCACCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGTTTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCTCCTCTAAACGTTCAGACAGCGTAATTCCAAATCCTTCACTCTTAAAGCTTCCCGCGCAGTGCAGGGGATGCTCtt                                                                                                                                                                                       <  1:278818/251‑1 (MQ=255)                                                                 tGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTAccc                                                                                                                                                                                                             >  1:396977/1‑223 (MQ=255)                                                                 tGGCCTGAAAAGGCGTTTACGCCGCACCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTAccc                                                                                                                                                                                                             <  2:396977/223‑1 (MQ=255)                                                                   gcctggtaagaggttcacgacgcAGCAGGCTGCAAGCACCGATTGCCGGATGCGGCGTAACCACATCCGGCGCAAAAACCTATCCCATCAGCGGGTTTTTCCCTTCACGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCTCGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAAAGGATGCTCGTTACGCACg                                                                                                                                                                               <  2:55041/231‑1 (MQ=255)                                                                    ccTGAAAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGt                                                                                                                                                                              <  2:90429/251‑1 (MQ=255)                                                                      tGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAACGCGATTGGCGGTAAACCAACCAGCGTGTTAGGATCAAGCCCCTCTAAAAGTTCAAACAGCGTAATGCCAAAACCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCAcata                                                                                                                                                                             >  2:117995/1‑247 (MQ=255)                                                                           aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATc                                                                                                                                                                       <  1:28785/251‑1 (MQ=255)                                                                           aGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACGATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATc                                                                                                                                                                       <  1:451679/251‑1 (MQ=255)                                                                                                  gccAGCACCGGTTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCCTCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCCAAACGTTCAAACAGCGTACTGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGAGTGATCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGAc                                                                                                                                                >  2:276819/1‑251 (MQ=255)                                                                                                              tGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACg                                                                                                                                     <  1:123139/250‑1 (MQ=255)                                                                                                                    aTGCGACGTAACCACACCCGTCGCAAAAAACTAGCCCATCAGCGGGTTTTGCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCTCTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAg                                                                                                                              <  1:2777/251‑1 (MQ=255)                                                                                                                          ggTAACCACATCCGGAGCAAAAAACTATCCCATCAGCGGTTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCa                                                                                                                        <  2:347424/250‑1 (MQ=255)                                                                                                                             aaCCACAGCCGGCGCAAAAAACTATCCCATCAGCGGGTTTCCCCTTTCCCGACGTAACATCTGACACATCGCGATTAGCTGTAAACCAACCAGCGTGTTAGGATCACGCCCCTTTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCGCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACtt                                                                                                                     <  2:167000/251‑1 (MQ=255)                                                                                                                                cacaTCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAACCAGCGTAATGCCAAATCCTTCACTATTAAAGCTACCCGCGCAGTGCAGGGGATGATCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTg                                                                                                                  >  2:242852/1‑251 (MQ=255)                                                                                                                                          gcAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATg                                                                                                        >  1:378885/1‑251 (MQ=255)                                                                                                                                                    aTCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAAGCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCg                                                                                                <  1:438191/249‑1 (MQ=255)                                                                                                                                                            aGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTaaa                                                                                      <  1:242852/251‑1 (MQ=255)                                                                                                                                                                     ttcccttcccGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGcc                                                                               >  2:22342/1‑249 (MQ=255)                                                                                                                                                                       cccttcccGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAACTGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGCATTAAACAACGccagtc                                                                           >  1:471221/1‑251 (MQ=255)                                                                                                                                                                         gatcccGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTC‑TTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGccagtccag                                                                        <  2:137379/249‑1 (MQ=255)                                                                                                                                                                              cGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTC‑TTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGccagtccag                                                                        >  1:137379/1‑246 (MQ=255)                                                                                                                                                                                    aaCATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGt                                                              >  2:260548/1‑251 (MQ=255)                                                                                                                                                                                         cTGACACAGCGCGATTAGCGGTAAACAAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGTAAATGGACGTCAAAAGGCTCCACTACTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCAAGTCAAGAAGGAGACg                                                          >  2:141929/1‑250 (MQ=255)                                                                                                                                                                                              acaGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTg                                                    <  1:464538/251‑1 (MQ=255)                                                                                                                                                                                               catcgcgATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTAAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGc                                                   <  2:116404/248‑1 (MQ=255)                                                                                                                                                                                                    cgATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTg                                              >  1:1155/1‑251 (MQ=255)                                                                                                                                                                                                                        aGCGTGTTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACg                          >  1:83147/1‑251 (MQ=255)                                                                                                                                                                                                                            tgtTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGc                       >  1:293972/1‑250 (MQ=255)                                                                                                                                                                                                                             gtTAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCAt                     <  1:374617/251‑1 (MQ=255)                                                                                                                                                                                                                              ttAGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGACTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCAtt                    >  2:422445/1‑251 (MQ=255)                                                                                                                                                                                                                                aGGATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCAttt                   <  2:136751/250‑1 (MQ=255)                                                                                                                                                                                                                                  gATCACGCCCCTCTAAACGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAAGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTttct                <  1:368906/251‑1 (MQ=255)                                                                                                                                                                                                                                          cccTCTAAACGTGCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCAGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTa         >  2:276513/1‑250 (MQ=255)                                                                                                                                                                                                                                                aaaCGTTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGttt  >  1:58569/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CGTTGTTTGCAAAAGCTGCGTAACGCGCGCTAAT‑ACAAGCATATAAACCTGATAATGGGCGGTTGGCCTGATAAGGCGTTTACGCCGCATCAGGCCGCCAGCACCGATTGCCGGATGCGACGTAACCACATCCGGCGCAAAAAACTATCCCATCAGCGGGTTTTTCCCTTCCCGACGTAACATCTGACACAGCGCGATTAGCGGTAAACCAACCAGCGTGTTAGGATCACGCCCCTCTAAACGCTCAAACAGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTT  >  NC_000913/1145867‑1146355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑