Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,974	C→T	A12V (GCT→GTT)	rluC →	23S rRNA pseudouridine(955/2504/2580) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,974	0	C	T	100.0%	122.6 / NA	39	A12V (GCT→GTT)	rluC	23S rRNA pseudouridine(955/2504/2580) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/18);  total (21/18)

CCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAA‑AAGGTGGCGGCG  >  NC_000913/1144751‑1145209                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             ccTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCAATTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACg                                                                                                                                                                                                                   <  1:214409/251‑1 (MQ=255)             aaGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTATTGCTGGTGATTGGTTTAATAACCATAAATACAGTGATCAAGCAGTGAAGTAATAAGGTGCAAAAGAAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCCCCGCTGACGAAGCGGGACAACGTATCGATAACtt                                                                                                                                                                                                       >  1:237125/1‑251 (MQ=255)                              ttATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTg                                                                                                                                                                                       >  2:460495/1‑250 (MQ=255)                                  cACCCGCTTACTCACAGTTTTGTCACTGCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGGAAAAATTGTTGTTATCACCGCTGACTAAGCGGGGCAACGTATCGATAACTTTTTGCGGACCCAATCGAAAgg                                                                                                                                                                                  <  2:337437/251‑1 (MQ=255)                                          tACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCa                                                                                                                                                                           <  1:269685/250‑1 (MQ=255)                                                     ttttCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGAt                                                                                                                                                                <  1:487963/250‑1 (MQ=255)                                                              cttGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTAtt                                                                                                                                                      >  1:473768/1‑251 (MQ=255)                                                                   tGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGCATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCg                                                                                                                                                 >  1:12171/1‑251 (MQ=255)                                                                        agtGGTTTAATAACCATCAAAAAAGTGAGCACGTAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCACCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAg                                                                                                                                            <  2:30461/249‑1 (MQ=255)                                                                                    aCCATCAATAAATGGAGAACGCAGGTAATTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTACCACCGCTGACGAAGCTGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGGATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCggg                                                                                                                                <  2:389754/251‑1 (MQ=255)                                                                                    aCCATCAATAAAGAGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGACACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGcacagggcggg                                                                                                                                >  1:417321/1‑241 (MQ=255)                                                                                      cATCCATAAAGTGATCACGCAGTGCAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGGAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTg                                                                                                                              >  2:320871/1‑251 (MQ=255)                                                                                         cAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAAc                                                                                                                           <  1:107618/251‑1 (MQ=255)                                                                                               agtgaaaaCGCAGGGAAGGAATAAAGTGAAAAAGTAAATATAAGCATAGAAAAATGAG‑AGGCGCGAATCCTGCTGGATAATTAGAATCGCTAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTTTTAGCACCGCTGACGAAGCGGTGCAACGTATTGATAACTTTTTGCGTACCCAATTAAAAGGCGTACCAAAAAGTATGATTTACCGTATTGTGCCTAAAGGCGAAGTGCGGGTGACCaaaaaa                                                                                                                     <  2:4312/244‑1 (MQ=255)                                                                                               aGTGATCACGCAGAGAAGTAATAAGGTGCAAAATTAAATATAAGCATAGAAAAATGAG‑TTGCGCGACTCTTGTTGTCTAATGAGAATCGCCAACCATGAAAACAGTGACTCCACCCGTAAAAATTGTGGTTATTACCTCTGACGAAGCGGGGCAACGTATCGATAACTTTGTGCGTATCCAATTGAAATGCGTACCAAAAAGTATGATTTACCTTTTTTGGCGTAAAGGCGAAGGGCGGTTGTACaaaaaa                                                                                                                     <  2:293613/251‑1 (MQ=255)                                                                                                gTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAAc                                                                                                                    <  2:352320/251‑1 (MQ=255)                                                                                                   aTCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGt                                                                                                                  >  2:211683/1‑250 (MQ=255)                                                                                                       cGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAACGTATGTTTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTa                                                                                                              >  2:160648/1‑250 (MQ=255)                                                                                                             gAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTg                                                                                                        <  2:472760/250‑1 (MQ=255)                                                                                                             gAAGTAATAAGGTGCAAAAGTAAATATAAGCAAAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTg                                                                                                        <  2:501887/250‑1 (MQ=255)                                                                                                              aaGTAATAAGGTGCAAAAGTAATGATAAGCATAGAAAAATGAGGTGG‑GCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTACCGATAACTTCTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGaa                                                                                                      <  2:376957/251‑1 (MQ=255)                                                                                                                    taaGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATaa                                                                                                 >  1:366566/1‑250 (MQ=255)                                                                                                                                gTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCgggg                                                                                    >  1:58509/1‑250 (MQ=255)                                                                                                                                                                  aTCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGGGATGAGGTGCGTATTCCACCGGTTCGCGTTGCt                                                   >  1:261184/1‑251 (MQ=255)                                                                                                                                                                    ccTGTTGGCTAATTAGAATCGCCAACCATGAACACAGGGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTCTCGATAACTTTTTGCGTACCCAATTGAAAGGCGAACCAAAACGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGACGCGGGGGATGAGGGGCGTATTCCACCGGTTCGCTTTGcggg                                                 >  2:118095/1‑248 (MQ=255)                                                                                                                                                                    ccTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTg                                                  >  2:135917/1‑250 (MQ=255)                                                                                                                                                                       gTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCg                                              <  1:211683/251‑1 (MQ=255)                                                                                                                                                                         tGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCACTTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGCAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGGGCGACTTCCACCGGTTAGCGTTGCTGataggg                                            >  2:81105/1‑246 (MQ=255)                                                                                                                                                                                  aGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAGGAAGAgg                                   >  2:100890/1‑251 (MQ=255)                                                                                                                                                                                  aGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTAGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGAACCCAATTGAAAGGCGTACCAAAAAGCATGATTTACCGTCTTTTGCGTAAAGGCGAAGTGCGGGGGAACAAAAAACGTATTAAGCCTGAATATAAACTCGATGCGGGTGATGAGGTGCGTATTCCACCGGTTAGCGTTCCTGAGCGGGCAGAAGAgg                                   >  2:271266/1‑251 (MQ=255)                                                                                                                                                                                             ccATGAAAACAGAGACGCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGATGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCTCCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCca                        <  1:131272/251‑1 (MQ=255)                                                                                                                                                                                               aTGAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGAATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCgggttttcccac                      >  2:322392/1‑241 (MQ=255)                                                                                                                                                                                                 gAAAACAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGAAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGAGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTTGCcaacc                     >  2:41640/1‑247 (MQ=255)                                                                                                                                                                                                    aaCAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTAt                                                                                                                                                       >  2:11545/1‑117 (MQ=255)                                                                                                                                                                                                    aaCAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTAt                                                                                                                                                       <  1:11545/117‑1 (MQ=255)                                                                                                                                                                                                      cAGAGACTCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCaa               <  1:322392/251‑1 (MQ=255)                                                                                                                                                                                                       agagACACCATCCGTAAAAATTGTTGTTAGCACCTCTGACGAATCGGGGCAACGTATCGATAACTTTTTGCGTTCCCAATTGAAAGGCGTACCTAAAAGTATGATTTACCTGATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCGGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATGCCACCGGTTCGCATTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCCCATCTGCaa‑a             <  2:426459/251‑1 (MQ=255)                                                                                                                                                                                                             tCCATCCGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACACTTGCAA‑AAggggg       >  1:434227/1‑248 (MQ=255)                                                                                                                                                                                                                   cGTAAAAATTGTTGTTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGGATTGCCACAACTGCAACAAGGTggggcgg  >  1:423067/1‑246 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             CCTTGTTATAGCAAGATGACTTTTACCATTTATCACCCGCTTACTCACAGTTTTTTCACTTCTTGCTGGTGATTGGTTTAATAACCATCAATAAAGTGATCACGCAGTGAAGTAATAAGGTGCAAAAGTAAATATAAGCATAGAAAAATGAG‑TGGCGCGAATCCTGTTGGCTAATTAGAATCGCCAACCATGAAAACAGAGACTCCATCCGTAAAAATTGTTGCTATCACCGCTGACGAAGCGGGGCAACGTATCGATAACTTTTTGCGTACCCAATTGAAAGGCGTACCAAAAAGTATGATTTACCGTATTTTGCGTAAAGGCGAAGTGCGGGTGAACAAAAAACGTATTAAGCCTGAATATAAACTCGAAGCGGGTGATGAGGTGCGTATTCCACCGGTTCGCGTTGCTGAGCGGGAAGAAGAGGCGGTTTCGCCACATCTGCAA‑AAGGTGGCGGCG  >  NC_000913/1144751‑1145209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑