Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,141,865 G→A P835S (CCG→TCG)  rne ← ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,8650GA97.7% 265.0 / ‑2.4 89P835S (CCG→TCG) rneribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (43/42);  total (46/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

TTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAACAAT  >  NC_000913/1141620‑1142108
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
ttCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGCTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACACCCGGTTCGATCGCGGCGGCGACAGGGACCTCAATCCCCATCGGCTGCCCATGCACTTCTTCCTGGTCGCGAT‑GCTCTTCCACCTGTGAC‑TATTGTGGCAGTAACATCTGG‑TAGCGGATCCAGACT‑TTGTCAGAAGCCAGCTCCGAAGAcg                                                                                                                                                                                                                                                 >  2:166185/1‑251 (MQ=255)
  cAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGGTCCGAAGAcgcg                                                                                                                                                                                                                                               >  1:190128/1‑251 (MQ=255)
     gaggCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAAACTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCAc                                                                                                                                                                                                                                            <  2:435084/248‑1 (MQ=255)
         cgtcgtTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTa                                                                                                                                                                                                                                        <  1:57101/251‑1 (MQ=255)
                 cAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCCTCGGCCGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGGCGTA‑CAATTGGATAGCGGTGCCCGACC‑TTGCCAGAGGCCAGGTCCGAAGACGCGCCCGCCACGGTCAAc                                                                                                                                                                                                                                >  2:9456/1‑251 (MQ=255)
                 cAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAAc                                                                                                                                                                                                                                <  1:351097/251‑1 (MQ=255)
                  aaCCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGGGGCCCGTTTCCAAGACGCGCACGcgcccggcaacg                                                                                                                                                                                                                               >  2:441251/1‑240 (MQ=255)
                         tCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCGACGACACCGGCCACTGCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTg                                                                                                                                                                                                                         <  2:190128/250‑1 (MQ=255)
                          cctgtggCGGTTCGGCAACCTGACCGGGGGCTTCTACGACACCGGCCACTGCTCCAACAACTGGCGCGCTAGCAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCGCGCTACGGTCAACGGCATTgg                                                                                                                                                                                                                        <  2:133340/244‑1 (MQ=255)
                           cGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCg                                                                                                                                                                                                                      >  1:88543/1‑251 (MQ=255)
                             gtggCGGTTCGGCATCCTGAACGGGGGCTTCTACGACACCGGCCCCTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGAc                                                                                                                                                                                                                    <  2:311823/248‑1 (MQ=255)
                                 cGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTAAACGGCATTGGCGACTggg                                                                                                                                                                                                                >  1:428779/1‑251 (MQ=255)
                                  ggatCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACCGGGACCTCAGTCACCATCGGCTGCACATGGACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CCAATGGGTAGCGGGTCCAGACC‑TTGCCAGTGGCCAGTTCCGAAAGCGCGCACGCTACCGTCAACGGCCTTGGCgcatgggg                                                                                                                                                                                                               >  2:109955/4‑244 (MQ=255)
                                              tGAACGGGGGCTTCTACGACACCGGCCCCTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTACCAGAGGCCCGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTc                                                                                                                                                                                                   >  1:385241/1‑251 (MQ=255)
                                                     gggCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGCCAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGTGGCCAGTTCCGAAGACGCGCACGCTACGGTCACCGGCATTGGCGACTGGGTTGGATACAGCTCGTCACg                                                                                                                                                                                             >  2:102569/1‑250 (MQ=255)
                                                      ggCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGAt                                                                                                                                                                                           <  1:266982/251‑1 (MQ=255)
                                                             aCGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGcgacg                                                                                                                                                                                    <  1:441251/251‑1 (MQ=255)
                                                                        cACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCCGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGGTCCCGACT‑TTGCCAGCGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCCTTGGCGACTGGGTTGGATAACGCTCGGCACGGTAGCGACGACGTCGCTggc                                                                                                                                                                         >  2:131580/1‑249 (MQ=255)
                                                                            ccttcAACAACTGGCGCGCAAACAACCGGTTCGATAGCGGGGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACGTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCa                                                                                                                                                                       <  2:301288/248‑1 (MQ=255)
                                                                              ttcAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCCTTGGCGACTGGGTTGGATAACGCTCGCCACGATAGCGACGACGACGCTGACCACtt                                                                                                                                                                    >  1:429971/1‑250 (MQ=255)
                                                                               tcAACAACTGGCGCGCTAACAACCGGTTCGATCGCGGCGGCGCCAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCTCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGGCTGGGTTGGATAACGCTCGTCACGGTAGCGACGACGACGCTGCCCACTTAc                                                                                                                                                                  >  1:183086/1‑251 (MQ=255)
                                                                               tcAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACt                                                                                                                                                                     <  1:153911/248‑1 (MQ=255)
                                                                                ccaaaacTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGTGCCCTCAGTACCCATCGGCTTCACATGTACTTCTTCCTGGTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACG‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTAc                                                                                                                                                                  <  2:127615/246‑1 (MQ=255)
                                                                                caacaaCTGGCGCGCTAACAACCTGTTCGATAGCGGCGGCGACAGTGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCTCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGTTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTAc                                                                                                                                                                  <  1:417001/250‑1 (MQ=255)
                                                                                 aacaacTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGGCCCCATCGGCCGCACATGTAATTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CCATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCCGTTCCGAAGAAGCGCACGCTCCGGTCAACGGCCTTGGCGACTGGGTTGGATAACGCTCGTCACGATGGcgagcaccagcgttcccaatttgtc                                                                                                                                                                >  2:249270/1‑229 (MQ=255)
                                                                                     acTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGCCGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCCGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATAGCGCCGACGACGCTGACCACTTACgcgc                                                                                                                                                              >  1:71247/1‑249 (MQ=255)
                                                                                     acTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAg                                                                                                                                                            <  1:256002/251‑1 (MQ=255)
                                                                                         gcgcgcTAAAAACCGGTTCGATAGCGGCGGCGACAGGGGCCTCAGTCACCATCGGCGGCACAGGTACGTCTTCCTGTTCGCGCG‑GCTCTTCAACCGGT‑CCATCGTGCGGACGTA‑CAATTGGATAGCGTATCCAGACG‑TTGCCAGAGGCCAGGTCCGAAGGCGCGCGCGCTACGGTCAACTTCAGTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGAAGACGCTGACCACGTACGCGCAGGTGa                                                                                                                                                        <  2:72720/251‑1 (MQ=255)
                                                                                             gcTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGACAGTTCCGAAGACGCGCCCGCTAAGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGCAGCTGACCACTTACGCTCAggggaag                                                                                                                                                      >  1:295263/1‑244 (MQ=255)
                                                                                                aacaacCGGTCCGATCGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTCCTTCCTGTTCGCGCT‑GCGCTGCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCCGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATATCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCg                                                                                                                                                 <  2:303108/251‑1 (MQ=255)
                                                                                                   aacCGGTTCGATAGCGGCGGCGACAGGGACCTCCGTCACCATCGGCTGCACATGTACGTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgc                                                                                                                                              <  2:467923/251‑1 (MQ=255)
                                                                                                   aacCGGTTCGATAGAGGCGGCGACCGGGCCCTCAGGCACTATCGGCTGCTCATGTTCTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTTT‑ACATCTTGCGGACGTT‑CAATTGGATAGCGGATCCAGACC‑TTGCCAGAGGCCAGTGCCGAAGACGCGCACGCTACGGTCAACGGTATTGGCTACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGa                                                                                                                                                <  2:412364/249‑1 (MQ=255)
                                                                                                    cccGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcg                                                                                                                                             <  2:211026/250‑1 (MQ=255)
                                                                                                     ccGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcgg                                                                                                                                            <  1:322177/251‑1 (MQ=255)
                                                                                                     ccGGTTCGATACCGGCGGCGACAGGGACCTCATTCACCATCGGCTGCACATGTACTTCTGCCTGTTTGCGCT‑GCTCTTCACCCTGT‑ACATCCTGCGGACGTA‑CAATTGGATATCGGAGCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAgcgg                                                                                                                                            <  2:362660/251‑1 (MQ=255)
                                                                                                      cGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCCGATCCAGACT‑TTGCCAGAAGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGCTCGTACCGCTAACGACGACGAACCGGGCCACTTACgcgccggggaaggggcgaggggg                                                                                                                                           >  2:481407/1‑232 (MQ=255)
                                                                                                             aTAGCGGCGGCGACAGGGACCTCAGTCAACATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAAGCCCGTTCCGAAGACGCGCACGCTACGGTCCACGGCATTTGCGACTTGGTTGGATCACGCTCGTCCCGATAGCTACGACGACGCTGACCACTTACGCGCCGGTGACATGGCGAGCGGCgagaccg                                                                                                                                    >  1:93395/1‑248 (MQ=255)
                                                                                                              tAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGCCGCTGTCCACTTACGCGCAGTTGACGAGGCGAGCGGCGAGAacg                                                                                                                                    >  1:105318/1‑250 (MQ=255)
                                                                                                                  ggcggcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CCATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAAAGGCATTGGCGACTGGGATGGATAACGCTCGTCACGATAGCGCCGCCGCCGCTGACCACTTACGCGCAGGTGACGACGCGAGCGGCCAGAccgacgac                                                                                                                               >  2:463282/1‑249 (MQ=255)
                                                                                                                      gcgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCAt                                                                                                                           <  1:217863/251‑1 (MQ=255)
                                                                                                                       cgACAGGGACCTCAGTCCCCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGCGGCGAGCGGCGAGAACGACGCGGCATg                                                                                                                          >  1:403683/1‑251 (MQ=255)
                                                                                                                       cgACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATg                                                                                                                          >  1:364934/1‑251 (MQ=255)
                                                                                                                        gACAGGGACCTCAGTCACCATCGGCTGCACCTGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGCCCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCaggc                                                                                                                         >  2:329244/1‑248 (MQ=255)
                                                                                                                         aCAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGcc                                                                                                                        >  1:41291/1‑251 (MQ=255)
                                                                                                                          cAGGGACCTCAGTCACCATCGGCTGCACGTGTACTTCTTCCTGTTCGCGCT‑GATCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGAATGGGCTGGATAACGCTCGTCACGATAGCGACGACGACGCTGAACACTTACGCGCCGGTGACGAGGCGTGCGGCGACAACGACGCGGCATGcc                                                                                                                        >  1:89112/1‑250 (MQ=255)
                                                                                                                          cAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGCGAACGACGCGGCATGcc                                                                                                                        >  1:1727/1‑250 (MQ=255)
                                                                                                                          cAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGCCGCGGCATGcc                                                                                                                        >  1:252269/1‑250 (MQ=255)
                                                                                                                            gggACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGG‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTGACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCAcc                                                                                                                     <  2:503099/251‑1 (MQ=255)
                                                                                                                             ggACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCg                                                                                                                    <  1:78160/251‑1 (MQ=255)
                                                                                                                               gccTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGtt                                                                                                                  <  2:208997/250‑1 (MQ=255)
                                                                                                                                 cTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTgg                                                                                                                >  2:463732/1‑250 (MQ=255)
                                                                                                                                     gtccccagcGGCTGCACATGGTCTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGAGCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACg                                                                                                            <  2:271333/243‑1 (MQ=255)
                                                                                                                                      tCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGCAT‑TTGCCAGAGGCCAGTTCCGAAGAAGCGCACGCTACGGTCAACGGCATTGGCAACTGGGTTGGATACCGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGCGCGGCGCGAACGACGCGGCATGCCACCGTTGTCACgg                                                                                                           >  1:461900/1‑251 (MQ=255)
                                                                                                                                                cTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGCGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCAt                                                                                                 >  1:307894/1‑251 (MQ=255)
                                                                                                                                                 tGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGCCGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCCCGTCACGCTAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGACAGAACGACGCGGCATGCCACCGTCGTCCCGGTTATCCGCAtt                                                                                                >  2:409930/1‑251 (MQ=255)
                                                                                                                                                   caaatttgctgtctcatGTTCGCGGT‑GCTCTCTTACCTTT‑ACAGCTTGCGGACGTA‑CAATTGGATAGCGGTTCCAG‑CTCTTGCAGGAGGCCCGTGCCGAAGACGCGCACGCTTCGGTCAATGGCATTGGCGACTGGGTTGGATAACGCTCGCCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTTACGATGCGAGCGGCGAGAACGACGCGGCATGCCAACGTTGTCACGGTTATCAGCATTga                                                                                              <  2:175799/235‑2 (MQ=255)
                                                                                                                                                    acaTGTTCTTCTTCCTGTTCGCGCT‑GTTCTTCATCCTGT‑ACATCTTTCGTACGTA‑CCATTGGATAGCGGATCCAGACT‑TTTCCAGAGGCCAGTTCCGAAGACGCGCACTCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATAGCGCCGCCTACGCTGACCACTTACGCGCAGGTGACGAGTCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGtt                                                                                             <  1:383492/251‑1 (MQ=255)
                                                                                                                                                     caTGTACTCCTTCCTGTTCTCGCT‑GCTCTTCAACCTGT‑ACATTCTGCGGACGTA‑CAAGTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTGCGGTCAACGGCATTGTCGACTGTGTTGGATAACGCTCGTCTCGATAGCGACTTCGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACTACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTtc                                                                                            <  1:177289/251‑1 (MQ=255)
                                                                                                                                                           cgtcttcCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCCCGCTACGGTCAACGGCAGTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCtcttct                                                                                      <  2:385241/249‑1 (MQ=255)
                                                                                                                                                             tcttcCTGGTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGCCGTA‑CAATTGGTTAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGCAGCCGCGCACGCTACGGTCAACGGCATTGGCGCCTGGGTTGGATAACGCTCGTCACGATCGCGACGACGACGCTGCCCACTTACGCGCAGGTGACGCGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATGTTTCTCTTCTTg                                                                                    >  2:24922/1‑251 (MQ=255)
                                                                                                                                                               ttcCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACg                                                                                                                                                                                                                                           <  2:446978/98‑1 (MQ=255)
                                                                                                                                                               ttcCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACg                                                                                                                                                                                                                                           >  1:446978/1‑98 (MQ=255)
                                                                                                                                                                tcCTGTTCGCGCG‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTg                                                                                    <  2:292924/248‑1 (MQ=255)
                                                                                                                                                                 ccTGTTCGCTCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGt                                                                                <  1:24445/251‑1 (MQ=255)
                                                                                                                                                                 ccTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGt                                                                                <  1:246580/251‑1 (MQ=255)
                                                                                                                                                                 ccTGTTCG‑GCTTGCTCTTCAACCTGT‑CAAGCTTGCGGACGTA‑CAATTGGAGAGCGGATCCAGACT‑TTGCCAGAGGCCAGGCCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCtt                                                                                 <  2:194745/250‑2 (MQ=255)
                                                                                                                                                                  ccgTTCGCGCT‑GCTCTTCAACCTGT‑TCATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTTCGGTCAACGTCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGCCCACTTTCGCGCAGGTGACGAGGCGAGCGGCGAGAACGCCGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGtt                                                                               <  2:405297/249‑1 (MQ=255)
                                                                                                                                                                       cgcgCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATCGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGTCAACCCGCACGCTACGGTCAACGGCCTTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGGCGACGACGCTGACCACTTACGCGCAGGTGACGGGGCGCGCGGCGAGAACGGCGCGGCCTGCCTCTGTGGTCCCGTGTGTCTGCATTGTTCTCTACTTGCTGTTCTgtt                                                                          >  2:40761/1‑249 (MQ=255)
                                                                                                                                                                          gct‑gctCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGTGCGGCGAGAACGACGCGGCCTGCCCCCGTTGTCACGGTTATCACCCTTGTTTTCTTCTTTCTGTTCTGGTGcg                                                                       >  2:223734/1‑250 (MQ=255)
                                                                                                                                                                           gcggctCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGAGCCAGACG‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGGTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTGACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTAGCAGCATTGTTCTCGTCTTGCTGTTCTGGTGCa                                                                       <  2:256806/248‑1 (MQ=255)
                                                                                                                                                                                  ttCAACCGGT‑ACATCTTGCGAAAGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTAAGGTCAACGGCATTGGCGACTGGGTTGGATAACGCGCGTCACGATAGCGACGACGACGCTGTCACCTTACGCGCAGGTGACGGGGCGAGAGGCGAGAACGACGCGGCATGCCACCGGTGTCACGGTTATAAGCATTGTTCTCTTTTTGCTGTTCTGGTGCCGTTTgcg                                                                >  2:277633/1‑251 (MQ=255)
                                                                                                                                                                                  ctcaaacctgctcagcgttcGGAAGTA‑CAAGTGGAAAGCGGATTCAGACT‑TTGCCAGTGGCCAGTTCCGAAGACGCTCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGACAACGCTCGGCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCTAGAACGACGCGGCATGCCTCCGTTGGCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGttt                                                                   <  2:77244/230‑1 (MQ=255)
                                                                                                                                                                                   ttaaCCTGT‑ACATCTTGCGGAAGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTg                                                                  <  2:402237/246‑1 (MQ=255)
                                                                                                                                                                                           t‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGCCGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCAACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTg                                                      >  1:98903/1‑251 (MQ=255)
                                                                                                                                                                                            gaCATCTGGCGGACGTA‑CAATTGGATAGCGGACCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATGGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGGTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTg                                                      <  2:105318/250‑1 (MQ=255)
                                                                                                                                                                                             aCATCTTGCGGACGTA‑TAAGTGGATAGCGGATCCAGTCT‑GTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTTCGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCCCTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTgg                                                     <  1:427160/251‑1 (MQ=255)
                                                                                                                                                                                                    gCGGACGTA‑AAATTGGAGAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATGGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGGTGTCACGGTTATCAGCATTGGTCTCTTCTTGCGGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCggg                                              <  2:183086/251‑1 (MQ=255)
                                                                                                                                                                                                      ggACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCGGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCTCAC‑ACTGGCAGCggggc                                            >  2:78984/1‑249 (MQ=255)
                                                                                                                                                                                                       gACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGTGCT‑ACGCCTGGCAGCGGGACt                                           >  1:293927/1‑251 (MQ=255)
                                                                                                                                                                                                          gTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTc                                        >  1:8488/1‑251 (MQ=255)
                                                                                                                                                                                                           tA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGATGTAACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTTCAGTTGGCGCT‑ACGACTGGCAGCGGGTCTTTc                                        >  2:339262/1‑250 (MQ=255)
                                                                                                                                                                                                                    gATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTAACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCCCCAGTTCt                               >  2:407426/1‑251 (MQ=255)
                                                                                                                                                                                                                         cGGATCCAGACC‑TTGCCAGAGGCCAGGTCCGAATACGCGCACGCTACCGTCAACGGCATTGGCGACGGGGTTGGATAACGCGCGTCACGATAGCGACGGCGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCg                          <  2:36792/251‑1 (MQ=255)
                                                                                                                                                                                                                               cAGACT‑TTGCCAGAGGACAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTTGCAGCGGGACTTTCCCCAGTTCTGTGCGTGGAGc                    >  2:55562/1‑251 (MQ=255)
                                                                                                                                                                                                                                      ttGCCAGAGGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGgc                <  1:463732/249‑1 (MQ=255)
                                                                                                                                                                                                                                            gagGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGGCTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTg        >  1:415976/1‑251 (MQ=255)
                                                                                                                                                                                                                                            gagGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGGGACGAGGCGAGCGGCGAGAACGACGCGGCCTGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTg        >  1:278250/1‑251 (MQ=255)
                                                                                                                                                                                                                                             agGCCAGTTCCGAAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTg        >  1:374774/1‑250 (MQ=255)
                                                                                                                                                                                                                                                  aGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAACAAt  >  1:147264/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
TTCAGGATGCGTCGTTTCAACCACTTCCGGTTGCGGTTCGGCAACCTGAACGGGGGCTTCTACGACACCGGCCACTTCTTCAACAACTGGCGCGCTAACAACCGGTTCGATAGCGGCGGCGACAGGGACCTCAGTCACCATCGGCTGCACATGTACTTCTTCCTGTTCGCGCT‑GCTCTTCAACCTGT‑ACATCTTGCGGACGTA‑CAATTGGATAGCGGATCCAGACT‑TTGCCAGAGGCCAGTTCCGGAGACGCGCACGCTACGGTCAACGGCATTGGCGACTGGGTTGGATAACGCTCGTCACGATAGCGACGACGACGCTGACCACTTACGCGCAGGTGACGAGGCGAGCGGCGAGAACGACGCGGCATGCCACCGTTGTCACGGTTATCAGCATTGTTCTCTTCTTGCTGTTCTGGTGCAGTTTGCGCT‑ACGACTGGCAGCGGGACTTTCACCAGTTCTGTGCGTGGAGCTGGCGCTTCCTGAACAAT  >  NC_000913/1141620‑1142108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑