| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,138,533 | C→T | G52G (GGC→GGT) | flgK → | flagellar hook‑filament junction protein 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,138,533 | 0 | C | T | 97.0% | 91.9 / ‑4.3 | 34 | G52G (GGC→GGT) | flgK | flagellar hook‑filament junction protein 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (19/13); total (21/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
TGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCA‑CCAGCCTGC‑AAACGCTGGTGAGTAACGCGGAAG‑ACCCGGCAGCGCGC > NC_000913/1138290‑1138767 | tGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCggttg‑ggttg > 1:303568/1‑251 (MQ=255) gAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTTTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGTATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCt < 1:176691/250‑1 (MQ=255) tAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCAATGGTGTC‑TACGTTTTTGgtg > 1:326269/1‑251 (MQ=255) cccGATAATACTTTGTAATTGAAGGCTTATAAGGAACCTCCATGGCCATCTTTATTAATAACGACATGAGCGGACTGAGCGCGGCCCAGGCGGCGTAAAATACGGAAAGAAATATTATCTTCAGCTAAAACTTTGCCGGAGATCCCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGttt < 2:271803/250‑1 (MQ=255) ataataCTCTGTAATTGAAGTTTTAAGAGGACCCTCCATGTCAAGCTTGATTAATAACGCCATGAGCGGACTGACCGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATGATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTttatta < 2:161799/251‑1 (MQ=255) taataCTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GCTTGGCAATGGGGTC‑TACTTTTCTGGTGGGCAGCGGGA‑GTATGAGTG‑GTttatta > 2:111277/1‑250 (MQ=255) taCTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGATTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGTGGTGGGCA‑TGGTGTA‑TACGTTTCTGGTGTGCGGCGAGG‑GGATTA‑TGCGTttattaaca > 1:199378/1‑247 (MQ=255) atAATGGAAGGATTATAAGGACCCCCCATGTCCAGCGTGATTAATAACGCCATGAGCGGAATGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTGGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGGGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGtt < 2:326269/250‑1 (MQ=255) aGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCAATTGGGTC‑CACGTTTCTGTTGTGTACAGTGG‑GTGTTA‑TGCGTTTATTACCAACCCGTCTCTTGCGGCGCAGACG‑c > 2:215545/1‑251 (MQ=255) ccTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGGGTC‑CACGTTTCTGGTGTGCCGCGGGA‑GTATGG‑TGGGTTTATTACCAACCAGTTACGTgcggcggcggcgcca > 2:499534/1‑239 (MQ=255) aggTCCAGCGTGATTAATAACTCCTTGATCGGATTGAACGCGCCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCAATAACGTTGCAGTATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCgg < 1:272966/249‑1 (MQ=255) aataaCGCCATGAGCGGACTGAACGCGGCCCAGCCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTGGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCt < 1:355095/250‑1 (MQ=255) ccATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATACTATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGATGGCAATTGGGTC‑TACGGTTCTGGTGTGCATCG‑GACGTATGA‑TGAGTTTTTTACCTACCAGTTACCTGCGGCGCCAACA‑CAACGTAGCGGTATGACCGCCCGCttagcgc > 1:61415/1‑243 (MQ=255) cATGAGAGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTG‑GGTTGGCAATGGAGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCCGACG‑CAAAGTTGCGGTCTTACTGCCCGcatagcgcca > 2:333849/1‑241 (MQ=255) ccAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAAACACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGCGTCATA‑GTTTCTGGTAGGCAGAGTGA‑GTATGG‑TGAGTTTATTACCAACCAGTTACGTGCGGCGCAGACGACAAA‑TAACGGTCTGACTGCACGCGCTGAGAAAGTGTCGAAAATCGACAAAATGc > 1:334221/1‑251 (MQ=255) aGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCa < 1:437286/145‑1 (MQ=255) cgTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGGTATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGACGCTGACGGGTG‑GGGTGGCTAGTGTGTC‑TCCGTTTGTGGGGTGCGGCGTGG‑GTAAGA‑TATATTTATTGCAAAACCGTTAAGTGCGGCGCCGGCG‑CCAAGTAGCGGGCTGTCTGCCCGCTTAGAGAAGATGGCGAACATCgtagaaaaggcagtcacc > 2:104467/1‑234 (MQ=255) cgTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCAGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGGGTC‑TACGGTTCTGGTGTGCAGCGTGA‑GTATGG‑TGCGTTTATTACCAACCCGTTACGTGGGGGGCACACG‑CAAAATAGCGATCTGGCTGCCCGCCATAAGCAGATGTCGAAAAtaggaaataagctccccccc > 2:305259/1‑232 (MQ=255) cGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCAAAGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCCGTTACGTGCGGCGCCGACG‑CAAAGTAGCGGGCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACctct > 1:486025/1‑251 (MQ=255) aataatATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGG‑GTATGA‑TGCGTTTATTACCAACCCGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTTACTGCCCGCTCTGAGCAGATGTCGAAAATTGAACATATGCTCTCCACCCATACCTCTTCGCTGGc > 1:395120/1‑251 (MQ=255) ccAGCTATAACGTTGCCGGATATCCCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATg < 1:147666/251‑1 (MQ=255) aGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGGGA‑Ggatga‑t > 1:457886/1‑123 (MQ=255) aCGTTGCCGAATATACCCGCCAAACCACTATGATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATttc < 1:104547/251‑1 (MQ=255) ccGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGAAAAAGTGTC‑TGCGTTTCTGGGGTGCAGCGGGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGAATGCCCGCTATGAGCAGATGTCGTAAATCGACAATATGCTCTCCACCAGAACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTTA‑cc > 1:325665/1‑251 (MQ=255) atatACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGGGCAGCGGGA‑GGATGA‑TGCGTTTATTACCACACAGTTACGTGCGGCGCAGACG‑CACAGTAGAGGTCTGACTGCCCGCCATGAGCAGATGTCGAAAATCAACACTACTATCTCCCCCAGTAACTCTTCCCTGGCAACACAGATGCAGGCTTTCTTTA‑CCaccc > 2:109591/1‑248 (MQ=255) cccGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGt < 1:158226/120‑1 (MQ=255) cccGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCAATGGTGTC‑TACCGTTCTGGGGTGCCGCGTGA‑GTATGG‑TGGGGTTATTACCAACCAGt > 2:158226/1‑120 (MQ=255) cGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCA‑CCAGCCTGC‑AAAc < 1:408216/251‑1 (MQ=255) gtcAAACCTCTTTTATGGCGCAGGCCAATAGCACGTGGGGCGCTGGCGGTTG‑GGGTGGCAATGGTGGC‑GACGGTTCTGGTGGGCAGCGGGA‑GTAGGA‑TGCGTTTATTAACATCCAGTTACGTGCGGCGCAGACG‑CATAGTAGCGGTCTGACTGCACGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTACCCCATTTCCTCTTCGCTGGCAACACGGATGCAGGATTTCTCCA‑CCAGCCTGC‑aaccg < 2:489243/249‑4 (MQ=255) aCCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCA‑CCAGCCTGC‑AAACGCTggg > 1:361648/1‑250 (MQ=255) atGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTATCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCA‑CCAGCCTGC‑AAACGCTGGTGAGTAAcg < 1:7982/250‑1 (MQ=255) ccAATAGCACGTTGGGCGCTGGCGGTTG‑GGGTGGCACTGGCTTC‑TACGCTTATGGTGTGAAGCGGGA‑GTATAA‑TGCGTTTATTACCACCCAGGTACGTTCGGCGCAGGCG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAAATGACAATATGCTCTCCACCAGTCACTCTTCGCTGGCACCACAGATTCAGGATTTTTTCA‑CCAGCCTGC‑AAAAGATGGAGCGTAACGCGGGAA‑ACCCg > 1:417519/1‑251 (MQ=255) cAATAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAATGGTGTC‑TACCTTTCTGGTGGGAAGCGGGG‑GTATGA‑TGGGGTTATTAACAACCAGTTACGTGCGGCGCAGAAG‑CAAAGGAGCGGTCTGACTGCCCCCTACGAGCAGGTGTCGGAAAACGACAATATGCTCTACACCCGTACCTCTTCGCTGGCAACACAAATGCAGGATTTCTTCA‑CCAGCCTGC‑AAACGCTGATGAGATACGCGGTAG‑ACTCgg > 1:266904/1‑251 (MQ=255) aaTAGCACGTTGGGCGCTGGCGGTTG‑GGTTGGCAAAGGTGTC‑TAAGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTTTTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGGCAATATGCTCTCCACCAGTACCTCTTCGCTGGCACCACAGATGCAGGATTTCTTCA‑CCCGCCTGC‑AAACGCTGGGGGGGAACACGGAAG‑ACCCGGc > 2:270109/1‑251 (MQ=255) cGTTGGGCGCTGGCGGTTG‑GGCTGGCAATGGTGTC‑TTCGTTTCTGGTGTGCCGCGTGA‑GGATGA‑TGCGTTTATTACCAACCAGTTACGCGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCCGATGTCGAAAATCGACAATATGACCTCCACCAGTACCTCTTCGCTGGCCACACAGTTGCAGGTTTTCTTCAACCA‑CCTGCCAAAC‑CTGGTGGGTAACCAGGAAGCACCCG‑CAgcgccc > 2:167382/1‑249 (MQ=255) | TGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTG‑GGTTGGCAATGGTGTC‑TACGTTTCTGGTGTGCAGCGTGA‑GTATGA‑TGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACG‑CAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCA‑CCAGCCTGC‑AAACGCTGGTGAGTAACGCGGAAG‑ACCCGGCAGCGCGC > NC_000913/1138290‑1138767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |