Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,466	C→T	A222V (GCC→GTC)	flgF →	flagellar basal‑body rod protein FlgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,466	0	C	T	96.2%	145.1 / NA	54	A222V (GCC→GTC)	flgF	flagellar basal‑body rod protein FlgF
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (16/34);  minor base A (2/0);  total (18/36)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTC  >  NC_000913/1134231‑1134705                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                tcGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGAACCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGttt                                                                                                                                                                                                                                  >  2:96676/1‑251 (MQ=255)  cGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACGGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGttt                                                                                                                                                                                                                                  <  1:337789/250‑1 (MQ=255)   ggCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTg                                                                                                                                                                                                                                <  1:239745/251‑1 (MQ=255)    gcgcTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTg                                                                                                                                                                                                                                >  1:297841/1‑250 (MQ=255)        ccAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATGTTTCGTTTAAGCGCAGAATCCCAGGCCACTCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATg                                                                                                                                                                                                                           <  1:295988/250‑1 (MQ=255)         cAATCCGGGCGATCCGGACCATAAGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGAGTGATGCCGGGGGTTCTGGAAGGCAGTAACGTCACTGCCGTTGCGGAAATGGGCGAAATGAGTGAAAGCGAGCGGCGGTTTGatatct                                                                                                                                                                                                                          >  2:373983/1‑246 (MQ=255)         cAATCCGGGCGAGCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCTAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAATCCCAGGCCTCGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATg                                                                                                                                                                                                                           <  1:344667/250‑1 (MQ=255)          aaTCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGc                                                                                                                                                                                                                          >  2:414020/1‑250 (MQ=255)           aTCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGc                                                                                                                                                                                                                          >  1:199142/1‑249 (MQ=255)                   gATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGt                                                                                                                                                                                                                <  1:366290/251‑1 (MQ=255)                   gATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGt                                                                                                                                                                                                                <  1:352338/251‑1 (MQ=255)                         gCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCAGTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGGGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATcag                                                                                                                                                                                                          <  2:391814/251‑1 (MQ=255)                          cAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATcagc                                                                                                                                                                                                         <  1:157518/251‑1 (MQ=255)                                        ccAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGACGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGAt                                                                                                                                                                                                                      >  2:469038/1‑224 (MQ=255)                                        ccAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCATGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTTATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGAt                                                                                                                                                                                                                      <  1:469038/224‑1 (MQ=255)                                          aGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACg                                                                                                                                                                                         <  1:253340/251‑1 (MQ=255)                                                 cGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTTACGACGGCATTGTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGGCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCTGGCCg                                                                                                                                                                                  <  2:29375/251‑1 (MQ=255)                                                   ccTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTTACGACGGCATTTTTCGTGTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTg                                                                                                                                                                                <  2:495733/250‑1 (MQ=255)                                                    actAAACGGGTGAAAGCCACGGGCTGCGAAGTGCAGCGCGGTGTCTACGGCATTTTTCGGTTATGCTCAGAAACACAGGCCACGCGGGGGCCGGTACTGCAGGCAGAGCCAACCTGGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATACCGCAGGCCGTg                                                                                                                                                                                <  2:91932/248‑1 (MQ=255)                                                                aaaGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAActgct                                                                                                                                                                   >  1:24239/1‑251 (MQ=255)                                                                  aGCCACGGGCTGCGAAGTGCAGCGCGGTGACGACGGCATTTTGCGTTTAAGCGCAGAAACCCAGGCCACGCGTGTGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGGTGATGATAACGCAGGCCGTGCCAACCAActgcggt                                                                                                                                                                 <  2:98574/251‑4 (MQ=255)                                                                          gCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGt                                                                                                                                                         <  2:342351/251‑1 (MQ=255)                                                                                 aGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGa                                                                                                                                                  <  2:99420/251‑1 (MQ=255)                                                                                         gcgGTGGCGACGGCATTTTTCGTTCAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGAAGGCAGCTCCAACATTGCGTGTGATGTCGGGGGTTCTGGCAGGCAGTACCGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGCTGAAGGTGATCAGCAGCGTCGATGCTAACGCATGCCGTGCCAACAAACTGCTGTCGATGAGTTAATTGAAAGGAtcc                                                                                                                                          >  2:184756/1‑249 (MQ=255)                                                                                           ggTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACAt                                                                                                                                        >  1:13235/1‑251 (MQ=255)                                                                                               acgacgGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGGCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACa                                                                                                                                    <  1:322180/251‑1 (MQ=255)                                                                                                  acgGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTATTTGAAAGGATACATgcca                                                                                                                                    >  2:67444/1‑245 (MQ=255)                                                                                                       aTTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCTGTCAGGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTTGAAGGCAGTAACGTCAATGCCGTGGCGGCAATGATCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATTAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGACGATGAGTTAATTGAAAGGATACATGACAAGTATAAg                                                                                                                            <  2:317467/251‑1 (MQ=255)                                                                                                       aTTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAg                                                                                                                                                                                      >  1:79304/1‑193 (MQ=255)                                                                                                       aTTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAg                                                                                                                                                                                      <  2:79304/193‑1 (MQ=255)                                                                                                            tCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGc                                                                                                                        <  1:322526/250‑1 (MQ=255)                                                                                                             cGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCCATGCCGTTGCGGCAATGAGCGACATGCTTGACAGCGCGCGGCGTTTTGCAATGCAGATGACGGTAATCAGCAGCGTCGATGATAACGCTGCCCGTCCCACCAAACTGCTGTCGATGAGTTAATAGAAAGGATACATGAAACGTATAAGTTGccc                                                                                                                      >  2:403714/1‑251 (MQ=255)                                                                                                             cGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGcc                                                                                                                       <  1:89932/250‑1 (MQ=255)                                                                                                               tttAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCg                                                                                                                     >  1:139240/1‑250 (MQ=255)                                                                                                                     cgcAGAAACACCGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAAACTTGAGGGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGTCAATGTGCGAAATGCTTGTCAGCGCGCGGCGATTTGAATTGCAGTTGAAGGTGATAAGCAGCGTCGCTGATAACGCAGGCCGGTCCAACCAACTGCAGAAGAGGATGCAATTGAAAGGATACATGACAAGaatagggtgaccgaagggc                                                                                                               >  2:253130/1‑235 (MQ=255)                                                                                                                         gAAACCCAGGCCACTCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGGGGGTGATGTCGGGGGTTCTGGAAGGCAGGAACGGCAATGCCGTGGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGGTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGGCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGtt                                                                                                          <  2:297841/251‑1 (MQ=255)                                                                                                                            aCCCAGGCCACGCGTGGGCCGGTTCTGCAGGCAGAACCAACCTTGCGTGTGAGGTCGGGGGTGCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCATTGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAATTTTAt                                                                                                       <  1:361041/251‑1 (MQ=255)                                                                                                                               cAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATAACAAGTATAAGTTGCCCGATGCGCAAGTTTATCgg                                                                                                    <  1:109273/251‑1 (MQ=255)                                                                                                                                   ccACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGCTGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCt                                                                                                >  2:69006/1‑251 (MQ=255)                                                                                                                                       gcgTGGGCCGGTACTGCAGGCAGATCCATCCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATgg                                                                                            <  1:491664/251‑1 (MQ=255)                                                                                                                                           gggCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGc                                                                                        >  1:407125/1‑251 (MQ=255)                                                                                                                                                tgTACTGCTGGCAGATCGAACCTTGCGTGGGATGGCTGCGGTTCTGTACGGCAGTAACGTCGATGCCGTTGCGGATATGAGCGACTAGATTGTCAGCTCTCGTTGTTTTGAAAGTCATATGAATGTGATCAGCGGCGTCGATGATAATGCAGGCCTTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAATTATAATTTGCCCGATTCGCAAGTTTATCGGGTCTATGTGGGCAAt                                                                                     <  2:395395/248‑1 (MQ=255)                                                                                                                                                   aCTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCa                                                                                 >  1:266349/1‑250 (MQ=255)                                                                                                                                                      gcaggcagATCCAACCTTGCTTGTGATGTCGGGGGGTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGAGATGATTGTCAGCTCGCGGCGTGTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGGTTATCGGGTCTATGGGGGCAATCGCAAttt                                                                             <  2:145246/251‑1 (MQ=255)                                                                                                                                                             gATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtt                                                                      <  2:123573/251‑1 (MQ=255)                                                                                                                                                              aTCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAttt                                                                     <  1:465563/251‑1 (MQ=255)                                                                                                                                                                                 gTCGGGGGGTCTGTAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGCCATGAGTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCg                                                  <  2:80272/251‑1 (MQ=255)                                                                                                                                                                                          tCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCTGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAATGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGc                                          <  1:387748/250‑1 (MQ=255)                                                                                                                                                                                             ggAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCg                                         <  2:139240/248‑1 (MQ=255)                                                                                                                                                                                                  gCAGTAACGGCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgcc                                 <  1:312717/251‑1 (MQ=255)                                                                                                                                                                                                   cAGTAACGTCAATGACGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccg                                <  1:286084/251‑1 (MQ=255)                                                                                                                                                                                                                         gCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGc          <  1:230223/251‑1 (MQ=255)                                                                                                                                                                                                                         gCAAAGAGAGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGCTGACGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACGAAGACATATGc          >  2:227447/1‑251 (MQ=255)                                                                                                                                                                                                                                 cGACATGAATGTCAGCGCGCGGCGTGTTGAAATGCAGAGGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc  <  2:155264/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                TCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTC  >  NC_000913/1134231‑1134705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑