| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,130,803 | C→T | A21T (GCG→ACG) | flgA ← | flagellar basal body P‑ring formation protein FlgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,130,803 | 0 | C | T | 97.2% | 96.9 / ‑4.6 | 36 | A21T (GCG→ACG) | flgA | flagellar basal body P‑ring formation protein FlgA |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (17/18); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01 | |||||||||||
CATCGCGGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTT > NC_000913/1130558‑1131048 | cATCGCGGCAACCACATAATGTCCGGTGGCCTGTACAGTAACCTGTAAATATCGGTTGTCGTTACCGCAGCGTGCCAACACACTCACATTGCCCCCCAGGCGGGAATTATTGCTCAGCGAAAGCAATGGCTGCTCTCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTTGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTAc < 2:502680/251‑1 (MQ=255) gcgGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTaa < 2:86788/251‑1 (MQ=255) aaCCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGAc < 1:333471/251‑1 (MQ=255) acagtAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCAc < 1:315555/247‑1 (MQ=255) cagtAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCGCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACg < 2:302884/248‑1 (MQ=255) aTTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGGTGCTCGCATGGCGGGAGGAGATTGGGCGCTGTGCGGATAGAAAAACAAACCTCATCACTTACCCCCCCGGGTTGGGCGCTAAAAAAATTGTGCAATTGCGGTGTGAGATTGCTCGTCGGACTTTACGGGATGAACAGTATCGCgat > 1:427981/1‑240 (MQ=255) aTTAACCTGTAAATATCGTTTGGCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCgat < 2:427981/240‑1 (MQ=255) ccTGTAAATATCGTGTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGt < 1:183448/251‑1 (MQ=255) cGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGccccc > 1:138384/1‑251 (MQ=255) cccAACACATGCCCATTGGCCCACAGGCGGGAATTATTGCGCAACGAAAGCAATGGCTGCTCGCCTGGCGGTAGTAGATTGGGCGCGGTACGAATAGAAACACGAACCGCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGGATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCAttc < 2:190621/250‑2 (MQ=255) attcacattGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑g < 1:89966/251‑1 (MQ=255) cacattGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTg < 1:349492/250‑1 (MQ=255) gCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGGAGGAGATTGGGCGCCGTACGAATAGAAAAACGAACCTCATCAATTACCCCCCCGCGTTGGGCGCTAAAAAAGTTGTGCAAATGCGATGTGAGATTGCTCGTCGTACTTAAAGGACTGAAAAGGATCGCGATGGTCGCCCCGCTACGTTTTATTTTCAGCATTTTTCCCCCCCGCCATTTTTACCAC‑GTGAAttttt > 1:230874/1‑248 (MQ=255) gAATTACTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGGAGATTGGGCGCTGGACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGGTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTGCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATg < 2:486665/251‑1 (MQ=255) aaTTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGGTGGGCGCTAAAAAAGTTGGGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCCTTTCTACAAC‑GTGAATTGTACCTTTCCGCAATgc > 2:384427/1‑250 (MQ=255) attGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCa < 1:199753/250‑1 (MQ=255) ttGCTCAGCGAAAGCAATGGCTTGTTGCAAGGCGGTAGTGGATTGGGTGCTGTACGAATAGAAACCCGAACCTCATCACTTTACCCTGTGAGTTGGGCGCTAAAAAAGTTGTGCAATGGCGATGTGAGATTGCTAGTCGGACTTAACGGACTGAACAGTAGCTCGATGATCGCCACGCTACTTTTTATTATCAGCATTTGCTCCCCCAGCAATTTCTTCAAC‑GTGAATTGTACCTGTCCGCAATGACCAt < 2:423771/250‑1 (MQ=255) gCTCATCGAAAGCAATGGCTGCTCGCAAGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCa > 1:189805/1‑250 (MQ=255) ccATCGAAAGCAAGGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACTCGAACCTCATCACTTCCCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACg < 2:248363/250‑1 (MQ=255) aaaGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAAACTCATCCCTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTTTCAGCATTTTCGCCCCCAGCCATTTCTACATC‑GTGAATTGTACATGTCCGCAATGACCATCACCGGCATa > 2:440056/1‑250 (MQ=255) aaGCAATGGCTGCTCGCATGGCGGTAGGAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCCCTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACATAATCGCGATGCTCGCCACGCGACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACCGGAAATTG‑ACCTGTCCGAAATGACCATCAACGGCATaa > 2:143648/1‑250 (MQ=255) tGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCg > 1:389832/1‑250 (MQ=255) gctCGCATGGCGGTAGTGGATTGGGCGCTGTACGAATAGAAAAACGAACATCATCACTTACCCCCGCGAATTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTAAGATTGCTCGTAGTACTTAACGGACTGAACAGTATAGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCTCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCCAGGACCATCAACGGCATAAATAGCCACCCAt > 2:458047/1‑251 (MQ=255) ctCGCCTGGCGGCAGTAGATTAGGCGCAGGACGAATAGAAAAACGACCCTCATCACTTACCCCAGCGAGGTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTTAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGAATATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTCCAAC‑GTGCATTGTACCCGTCCGCAATTACCATAAACGGCATAAATAGAGAcacctt > 2:398226/1‑246 (MQ=255) aCGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGt < 1:224731/251‑1 (MQ=255) aGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGc < 1:124213/251‑1 (MQ=255) aaaCACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCAt > 1:477473/1‑251 (MQ=255) ccttccccccGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGAGTGCCCGTCGTTCTTAACGGCCTGAACAGTAACGCGATTATCGCCACGCTACTTTTTATTATCAGCATTTTCCCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCAACAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGa < 2:230874/246‑1 (MQ=255) ccTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTCCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGa < 1:384427/250‑1 (MQ=255) tACCCCCGCAAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGCTGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGAGCGTAAAGGCATTTAAGCTGATGGCAGAAtt > 2:60983/1‑251 (MQ=255) cccccGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTCTTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAAtttt > 2:304868/1‑251 (MQ=255) cgcTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGACTTTTGATACCTGCggaggg > 2:253288/1‑250 (MQ=255) cgcTAAAAAAGTGGTGCAATTGCGATGTGAGAGTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCggagga < 1:263297/251‑1 (MQ=255) tAAAACAGTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGACCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATCAATAGCGACCCATTTTGCGTTTATTACGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCggaggcga > 2:467360/1‑247 (MQ=255) aTGTGAGATTGCTCGTCGTACTTAACGGACTAAACAGTATCGCGCTGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCACCGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGCGGAGATATGCTCGATAAGCTCGAcgcc > 2:504414/1‑251 (MQ=255) gCTCGTCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGtt > 1:307904/1‑251 (MQ=255) | CATCGCGGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAAC‑GTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTT > NC_000913/1130558‑1131048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |