Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,130,210	G→A	N218N (AAC→AAT)	flgA ←	flagellar basal body P‑ring formation protein FlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,130,210	0	G	A	100.0%	92.4 / NA	30	N218N (AAC→AAT)	flgA	flagellar basal body P‑ring formation protein FlgA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/22);  total (8/22)

TCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTT  >  NC_000913/1129978‑1130418                                                                                                                                                                                                                                         |                                                                                                                                                                                                                 acAGTTTTGCTTGCGCGTCGTTTAACGTCTCACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCa                                                                                                                                                                                                <  1:200283/250‑1 (MQ=255)  cAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCAt                                                                                                                                                                                               <  2:314695/251‑1 (MQ=255)               gcgTCGCTTAACGTCACGCTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCaacaac                                                                                                                                                                                  <  1:177411/251‑1 (MQ=255)               gcgTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCaacaac                                                                                                                                                                                  <  1:191141/251‑1 (MQ=255)                  gcGCTTAACGTCACACTTGTGCTGGTGGAGGCTGTTGTTTTTGCCGCCCGTCTGTTCGTTGCCGGCGCGGCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATGCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGTCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAAcgcc                                                                                                                                                                               <  2:4960/250‑1 (MQ=255)                             cacaCTGGGGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCt                                                                                                                                                                    <  2:238598/251‑1 (MQ=255)                                                          ttGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCAtt                                                                                                                                        <  1:494967/250‑1 (MQ=255)                                                           tGCCGCCCGGGTGTGCGTTGCCGGCGCGGCAGTGGGTGCGCGCGGTTGAACGGTGCGTACAGGCTTCAGAGGCGAAGTGCGATCAAGACGCATGGTTTATTCCTCAGTGAGGGCGCTTTTATCATGTGTTGCTTATTTAGCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGAGTTATAAGAATATTCCCATCTGCAGCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTc                                                                                                                                       <  2:48486/250‑1 (MQ=255)                                                           ggccgccCGGCGGTTCGTTACCGGTGCGTCAGGGGTGTCGCGCGGTTGAACGGTGCTTACAGGCTGCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGTATTCt                                                                                                                                      <  2:224422/250‑1 (MQ=255)                                                                   ggttGTTCGTTACCGGCGCGTCAGTGGTGTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACg                                                                                                                              <  2:141360/248‑1 (MQ=255)                                                                   ggCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACg                                                                                                                              >  1:273433/1‑251 (MQ=255)                                                                          cGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCa                                                                                                                        <  1:313973/250‑1 (MQ=255)                                                                             gtccggctagTCAGTGGTTTCGTGCGGTTGAATGGTTCTTACAGGCTTCAGAGGCGAAGTGCGAGCAAGACTCATGGTTTATCCCTCAGTGAGGGCGCTTATATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATACGAATATTCCCATCTGCTTCAACAACGACGCTGACGACCTGTCCCGATCCCATGCGCACCCGCGCAGTCTGTGCGACGGCTGCATTg                                                                                                                     <  2:8943/241‑1 (MQ=255)                                                                                 ggCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAg                                                                                                                <  1:18074/251‑1 (MQ=255)                                                                                     cgTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCCATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGTACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTAACTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGcc                                                                                                            >  2:433531/1‑251 (MQ=255)                                                                                          gggggttCGCGCGGTTGAACGGTGCTTACAGGCTTCGGAGGCGAAGTGCGATCTATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCCCCTGAcc                                                                                                       <  2:400268/246‑1 (MQ=255)                                                                                                                     aCAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCAcc                                                                            <  1:328229/251‑1 (MQ=255)                                                                                                                                agGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATc                                                                  >  1:405417/1‑250 (MQ=255)                                                                                                                                             tCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTg                                                                                                                                                                           <  2:287267/132‑1 (MQ=255)                                                                                                                                             tCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTg                                                                                                                                                                           >  1:287267/1‑132 (MQ=255)                                                                                                                                                aTACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCATCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTGGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTcc                                                 <  1:284914/251‑1 (MQ=255)                                                                                                                                                  aCTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCg                                               >  1:106929/1‑251 (MQ=255)                                                                                                                                                    tCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGGTGCATTGTTCAGCGCCTGACCTTCt                                                                                                   <  1:150114/197‑1 (MQ=255)                                                                                                                                                        ggtgtATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAAGCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGGATTGTTCAGCGCCTGACCTTCTGCGTTGGCTCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCtttg                                          <  1:460445/246‑2 (MQ=255)                                                                                                                                                        ggTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCtttt                                          <  1:460459/250‑1 (MQ=255)                                                                                                                                                         gTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAc                                        >  1:190621/1‑251 (MQ=255)                                                                                                                                                               tCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCAc                                                                             <  2:275362/208‑1 (MQ=255)                                                                                                                                                               tCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCAc                                                                             >  1:275362/1‑208 (MQ=255)                                                                                                                                                                                       ggtgtTGCTTATTTAACGGAAAGGGAGGGGTAATCTTAAACGTCTTAAAGATTTATAAGAATATTCCCATCTGCACCAACAACGCCGCTGATGACCTGCCCCGATCCCATGCGCCCCCGCGCATTCGGTGCGACGGCTGCATTGTTCAGCGCCTGCCTTTCTGGGTTGGCGCAAACCCCAACACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTCCCCGCCATGCCTGGCGAAACTGGGTTaa             <  1:100216/247‑1 (MQ=255)                                                                                                                                                                                               ttatttatCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGtt  >  1:295357/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                 TCAGTTTTGCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTT  >  NC_000913/1129978‑1130418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑