Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,126,518	C→T	A121V (GCC→GTC)	yceH →	DUF480 domain‑containing protein YceH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,126,518	0	C	T	100.0%	143.7 / NA	45	A121V (GCC→GTC)	yceH	DUF480 domain‑containing protein YceH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

CAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAA  >  NC_000913/1126274‑1126764                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         cAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTcgcgcg                                                                                                                                                                                                                                                    >  1:165121/1‑251 (MQ=255)      aaCGAACCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCCGGGTAACTGCGCAGCCGCGTCGCTCGAATGt                                                                                                                                                                                                                                               >  2:334772/1‑251 (MQ=255)           aCCGTGAACCGGTCATGAATCTGAGTGAATCCAAAGTGCAGTAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGaa                                                                                                                                                                                                                                          <  2:124132/251‑1 (MQ=255)                gAACCGGTCATGAATCTGAGTGAATCCAAAGTGCCGCAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCCGACGCCAGGTGCACTGCGCAGCCGCGTCGCGCGAATGTATGAATTc                                                                                                                                                                                                                                       >  2:306480/1‑249 (MQ=255)                       tCATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATg                                                                                                                                                                                                                              >  1:156219/1‑251 (MQ=255)                        cATGAATCTGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATgg                                                                                                                                                                                                                             >  1:479728/1‑251 (MQ=255)                             attctaattaagacaaagttccggAACAGCTGGATAATCAGGGCAAAAAGTTAT‑AGCTGCGCAGAGTGAGGGGTTTTGGTAAACAGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGGGGCGT‑TAATCAGCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCTGCCGCGTCGCGCTAATGTATGAATTCAGCGATATGGCGGaa                                                                                                                                                                                                                        <  2:498578/229‑1 (MQ=255)                                tGAGTGAATCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGCAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAggg                                                                                                                                                                                                                     >  1:42381/1‑249 (MQ=255)                                       aTCCAAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCgc                                                                                                                                                                                                              >  1:368089/1‑250 (MQ=255)                                            aaGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTg                                                                                                                                                                                                         >  1:342724/1‑251 (MQ=255)                                              gggCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTAGTTACGCACAGTGAGCGGTTTGGGGAAGCGGGTCACCAAATATGAGCAACGTTTGTGTAATTCAGAATGTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGa                                                                                                                                                                                                       <  2:482977/249‑1 (MQ=255)                                                    gAACAGCTGGATATTCTGGTCACAC‑GTCATTATCTACGCACAGTGAGCTGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAATCTTAGCGCAGCGGAAGTGGCGT‑TAATCACCTCGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCTCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGATTCTGGAACAACt                                                                                                                                                                                                 <  2:307193/251‑1 (MQ=255)                                                                       tCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCg                                                                                                                                                                                                                                   <  1:369639/198‑1 (MQ=255)                                                                       tCAAAC‑GTCATTATCTACGCACAGAGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCg                                                                                                                                                                                                                                   >  2:369639/1‑198 (MQ=255)                                                                         aaaC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAACTATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGCCGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCCGCGATATGGCGGAAGGGGGGTGGACGCTGGAACAACTGGCAAAACGCGTAGagtggac                                                                                                                                                                            >  2:324116/1‑245 (MQ=255)                                                                            c‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCttt                                                                                                                                                                         >  1:220415/1‑251 (MQ=255)                                                                                 aTTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGGGGCGGCGCTGGGACAACTGGCAAATCGCGAAGGTGGTCCTTTtgtg                                                                                                                                                                     >  2:217688/1‑251 (MQ=255)                                                                                      cTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGGGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGAATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGGACAACTGGCAAATAGCGAAGATGGTCCTTTTgtggtggg                                                                                                                                                                >  1:65042/1‑249 (MQ=255)                                                                                         cGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGACGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCt                                                                                                                                                             >  1:445409/1‑251 (MQ=255)                                                                                                 gAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCcgcg                                                                                                                                                     >  1:9998/1‑251 (MQ=255)                                                                                                     ggTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGACGCTGGAAAAAAAGGCAAATCGCGAAGGTGGTCCTTTTGTTGAGCGGCTGGCCCGCGAAcc                                                                                                                                                 >  1:298748/1‑251 (MQ=255)                                                                                                                 cGGGTCACCAAATATGGGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGa                                                                                                                                     <  2:505853/251‑1 (MQ=255)                                                                                                                      cACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAAcc                                                                                                                                <  1:306480/251‑1 (MQ=255)                                                                                                                              atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATg                                                                                                                      <  1:13624/251‑1 (MQ=255)                                                                                                                              atGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATAACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGAGATATGGCGGAAATGGTGTCGAAGCAGGAACAAATGGGAAAACCCGAAGATGGTACTTTTGTGGTGAGTCTGGCCCGCGCAACGGGtcgaagcgataacagctaactt                                                                                                                        >  2:414844/1‑230 (MQ=255)                                                                                                                                   cAACGTTTTTGTAAATCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGGTATTGCGTGGTGCCCAGACGCCAGGGGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCCTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCagct                                                                                                                 <  2:98788/251‑4 (MQ=255)                                                                                                                                         ttttGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCa                                                                                                            <  1:247505/250‑1 (MQ=255)                                                                                                                                          tttGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAg                                                                                                           <  2:139017/250‑1 (MQ=255)                                                                                                                                                   cAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAACCGCGTCGCGCGAATGTATGAATTCAGCGGTATGGCGGCAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCACGCACCGGGTAAACGCGAAAACCGC‑CA‑CACGCATCTTTTCAGCGGAGAGGtt                                                                                                 >  1:164155/1‑251 (MQ=255)                                                                                                                                                      aaTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGGACAACTGGCACATCGCGCAGATGGTCATTTTGTGGTACGTTCGGCCCGCGGAACGGGGAAAAGAGAAAACAGCGTA‑‑ATGCATCTTTTCAgtgggggggtttg                                                                                               >  2:473843/1‑241 (MQ=255)                                                                                                                                                      aaTTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATTCATCTTTTCAGTGGTGGGGTTGa                                                                                               >  1:95103/1‑250 (MQ=255)                                                                                                                                                              gATCTGAAACTGCGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGGCGCTGGAACAACTGGCAAATCGCGGAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGGAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCCGGGGTGGGGTTGAAGATGAGcc                                                                                      >  1:117835/1‑251 (MQ=255)                                                                                                                                                                   gAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGcc                                                                                      <  2:220415/246‑1 (MQ=255)                                                                                                                                                                     aaCTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTg                                                                               <  1:217688/251‑1 (MQ=255)                                                                                                                                                                        ggagagcAGCGGAAGTGG‑GTGTAACCCCCACGTTGTGATGTCGTGGTGCCCAGACGCCAGGTGAACTGCGCATCCGCGTCGCGCGAATGTATGAATCCAGCGATATGGCGGAAGTTGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGCGCGTCTGGCCCGCGAACCGGGGAAACGCGAAAACCGC‑TA‑CATGCTTCTTTTCAGTGGTGAGGTTGAAGATCAGCCGTCGGTGACg                                                                            <  2:240841/246‑1 (MQ=255)                                                                                                                                                                        ggAGCGCAGCGGAAGTGGCGT‑TAATCACCCCGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACg                                                                            <  2:156219/250‑1 (MQ=255)                                                                                                                                                                         gAGCGCAGCGGAAGTGGCGT‑TAAGCTCCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑GA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGCCgg                                                                           <  2:136340/251‑1 (MQ=255)                                                                                                                                                                              cAGCGGAAGTGGCGT‑TACTCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCCAAAACCGC‑TA‑CCTCCATCTTTTCCGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGatatt                                                                      >  1:418238/1‑250 (MQ=255)                                                                                                                                                                                 cGGAAGAGGCGT‑TAAGCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTCTGAATTCAGCGATATGGCGGACATGGAGTCGGCGCTGGAAACACTGGCCAATCGCGAAGATGGGCCTTTTGTGGTGCGTCTTGCCCACGAACCGGGTGAACTCGGAAACCGC‑TAACTTCCA‑CTTTTCAGTGGGTAGGTTGAAGAACAgacgtcgggggcgggtattttg                                                                   >  2:472407/1‑230 (MQ=255)                                                                                                                                                                                       tGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGACTTCAGCGATATGGCGGAAGTGGCGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTATGGCCCGCGAACCGGGTAAACGCGAAAACCGA‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGGGACGGATATGTCGGAcgcg                                                             >  1:454743/1‑251 (MQ=255)                                                                                                                                                                                          cGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGGGTCGACGCTGGAACAACTGGAAAATCGCGAAGATGGGCCTTTTGTGGGGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGGGAGGGTGAAGATCAGCCGGCGGGGACGGGTATGTGGAACGCgggt                                                          >  1:180333/1‑249 (MQ=255)                                                                                                                                                                                                         ttgttATTGCGTGGTGCCCAGGCGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGCAGTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGCACAACTGGCAAATCGCGAAGAGGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCAGCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTa                                              <  2:378338/249‑1 (MQ=255)                                                                                                                                                                                                                    tggtgCCCAGACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGGGATTTACAGGCCcgcggcg                                 >  1:465097/1‑248 (MQ=255)                                                                                                                                                                                                                             gACGCCAGGTGAACTGCGCAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTgg                        <  2:479728/251‑1 (MQ=255)                                                                                                                                                                                                                                            gcgcAGCCGCGTCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGGGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCTCGTCGAAGCCCTGGAAATCGAAGTGGCa          <  1:198755/250‑1 (MQ=255)                                                                                                                                                                                                                                                   cgcgtcgcggGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCGGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGaa  <  1:356262/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         CAGAAAACGAACCGTGAACCGGTCATGAATCTGAGTGAATCCGAAGTGCAGGAACAGCTGGATAATCTGGTCAAAC‑GTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGT‑TAATCACCACGTTGTTATTGCGTGGTGCCCAGACGCCAGGTGAACTGCGCAGCCGCGCCGCGCGAATGTATGAATTCAGCGATATGGCGGAAGTGGAGTCGACGCTGGAACAACTGGCAAATCGCGAAGATGGTCCTTTTGTGGTGCGTCTGGCCCGCGAACCGGGTAAACGCGAAAACCGC‑TA‑CATGCATCTTTTCAGTGGTGAGGTTGAAGATCAGCCGGCGGTGACGGATATGTCGAACGCGGTTGACGGTGATTTACAGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAA  >  NC_000913/1126274‑1126764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑