Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,120,111	G→A	A159V (GCG→GTG)	solA ←	N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,120,111	0	G	A	97.9%	148.2 / NA	48	A159V (GCG→GTG)	solA	N‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (25/22);  minor base C (0/1);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTT  >  NC_000913/1119866‑1120355                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cACCGGTAAACGCCGTGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTGTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAg                                                                                                                                                                                                                                                   <  1:458932/250‑1 (MQ=255)   ccGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTg                                                                                                                                                                                                                                                >  1:51356/1‑251 (MQ=255)     ggTAAACGCCGGGAATTTATTCTTCACGCTAGAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGTGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGa                                                                                                                                                                                                                                              <  1:312058/251‑1 (MQ=255)     ggTAAACGACGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGGGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTgg                                                                                                                                                                                                                                               >  2:148370/1‑250 (MQ=255)                                 cTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCCG‑TTCGCTgcg                                                                                                                                                                                                                 >  2:470499/1‑251 (MQ=255)                                  tataGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATCGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTgcgc                                                                                                                                                                                                                >  2:180970/1‑251 (MQ=255)                                   ataGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGACTTGCGGTGACCGGGCAGTTGAACAGTTGCGCACCGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTgcgc                                                                                                                                                                                                                >  1:124302/1‑250 (MQ=255)                                     aGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCaa                                                                                                                                                                                                              >  2:191597/1‑250 (MQ=255)                                        ggCCAACGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTGCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCaaaaaa                                                                                                                                                                                                          <  2:205275/251‑1 (MQ=255)                                              cGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGaa                                                                                                                                                                                                    <  2:410720/251‑1 (MQ=255)                                                            gCAAATACTTTGCGTACGGGCTGGACAGGCAGCGCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTGCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATaa                                                                                                                                                                                      <  2:400790/251‑1 (MQ=255)                                                                aTACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCg                                                                                                                                                                                  <  2:199713/251‑1 (MQ=255)                                                                                             cccGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCgg                                                                                                                                                     <  2:399062/250‑1 (MQ=255)                                                                                                    gCAGGTCTTTTACCCATGTTCCCGCGCAGACACTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCAATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTACTTCACCAGTTGGATCCAGGTTTTAATCGCCCG‑TTCGCTGCGCAAAAAACAGGCATCAGTTTCACATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCgg                                                                                                                                              >  2:279515/1‑251 (MQ=255)                                                                                                     cAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCggg                                                                                                                                             <  2:34218/251‑1 (MQ=255)                                                                                                     cAGGTCTGTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATTACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCggg                                                                                                                                             <  1:452333/251‑1 (MQ=255)                                                                                                                   aTGTTCCCGCGCAGACAATCGCTTTTTTAGCCTGATACTCCCCGTCAACCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGATTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGGATCAATTTCAACTAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGTGGGCCCTAATCCCGTgc                                                                                                                               >  2:505190/1‑251 (MQ=255)                                                                                                                        cccGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCACAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCAt                                                                                                                           >  1:300360/1‑250 (MQ=255)                                                                                                                         ccGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCg                                                                                                                         >  2:324090/1‑251 (MQ=255)                                                                                                                           gcgcTGACAATCGCTGTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCCCAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAg                                                                                                                       <  2:441284/251‑1 (MQ=255)                                                                                                                           gcgcAGGCAATAGCTTTGTTCGCCTGTTACTCACCGTCAGCCGCTTCAATAGTTACGACCTCATAGGCATGACACATTGCGGTGACCGGGCCGTCGAACCGATGCCCACACCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTCATCGC‑AGATTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGGATTTACGCCCAGCGGACCCTAATCCACTGCGCAtaggg                                                                                                                       >  2:321921/1‑247 (MQ=255)                                                                                                                             gcAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAtt                                                                                                                                                          >  2:322141/1‑214 (MQ=255)                                                                                                                             gcAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTAtt                                                                                                                                                          <  1:322141/214‑1 (MQ=255)                                                                                                                                   aaTCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCa                                                                                                                >  1:178208/1‑250 (MQ=255)                                                                                                                                        cTTTTTTCGCCTGATACTCACCGTCAGCCGTTTTAATAGTTACGCCATCCTCGTCCTGACGAATTGCGGTGACCGGGCAGTTGAAAAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAAACGGAATCAGTTTCAAATAAGCCGATGAAGTTGTCCGGGACGCGTATTTCAGGCAAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTg                                                                                                          >  2:322952/1‑251 (MQ=255)                                                                                                                                        cTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAACAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGGGCTTTTCAACGTTg                                                                                                          >  2:382783/1‑251 (MQ=255)                                                                                                                                           ttttCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGCTGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCACTTGCGCATCTGGCTTTTCAACGTTGAGt                                                                                                       >  2:351367/1‑251 (MQ=255)                                                                                                                                                 ccTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCAt                                                                                                 <  1:242324/251‑1 (MQ=255)                                                                                                                                                     aTACTCACCGGCAGCCGTTACAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGGTGCCATTggt                                                                                             <  2:43012/251‑3 (MQ=255)                                                                                                                                                         tCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGgc                                                                                         >  1:232364/1‑251 (MQ=255)                                                                                                                                                           aCCGTCAGCCGTTTCACTAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGgcgc                                                                                       >  1:367560/1‑251 (MQ=255)                                                                                                                                                            ccGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCt                                                                                      <  1:240360/251‑1 (MQ=255)                                                                                                                                                                     gTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGGCCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGGGGGCGAc                                                                             >  2:46715/1‑251 (MQ=255)                                                                                                                                                                     gTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGAc                                                                             <  1:382783/251‑1 (MQ=255)                                                                                                                                                                         cAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGtt                                                                          <  1:133431/250‑1 (MQ=255)                                                                                                                                                                                          tcGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATc                                                        >  1:393149/1‑251 (MQ=255)                                                                                                                                                                                           cGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa                                                       <  1:191597/251‑1 (MQ=255)                                                                                                                                                                                                 gACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCATCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGGTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCggg                                                 <  2:79647/251‑1 (MQ=255)                                                                                                                                                                                                              gACCGGGCAGTTGAACAGTTGCGCACCGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATg                                     >  1:209744/1‑250 (MQ=255)                                                                                                                                                                                                                  gggCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCgg                                                                                                                                                     <  2:261388/134‑1 (MQ=255)                                                                                                                                                                                                                  gggCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCgg                                                                                                                                                     >  1:261388/1‑134 (MQ=255)                                                                                                                                                                                                                         tGAACAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACTCCAGAGCGt                         >  1:43434/1‑251 (MQ=255)                                                                                                                                                                                                                            aCAGTTGCGCACAGCCCGCTTCCTTCACCAGTTGGTGCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACa                      <  2:198716/251‑1 (MQ=255)                                                                                                                                                                                                                                 gtccctcatcccGCTGCCTACACCAGTGGGATCCAGGTTTAAACCGCCGG‑TTCGCTGCGCAAAAAACCTGAATCAGTTTCAAACACGCCGATTTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCAGCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTAGAATCAGCCAGGCCAAGTTTAATGACGCCAGAGCGTACAAAAAt                 <  2:67617/242‑1 (MQ=255)                                                                                                                                                                                                                                       caGCCCGCTTCCTTCCCCAGGTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtc           <  2:457028/251‑1 (MQ=255)                                                                                                                                                                                                                                         gCCCGCTTCCTTCACCAGTTGGATCCAGGGTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtctt         <  1:470499/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccGCTTCCTTCACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAACACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATCATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGACGGGCCAAGGTTAATGACGCCAGAGCGTAAAAAAATGGGAtcttct       >  2:85527/1‑251 (MQ=255)                                                                                                                                                                                                                                                tccttcACCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGtt  >  1:504704/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CACCGGTAAACGCCGGGAATTTATTCTTCACGCTATAGCGGCCATCGGCCTGATACCAGGCAAATACTTTGCGTACGGGCTGGACAGGCAGCTCCGGGAGCAGGTCTTTTACCCATGTTCCCGCGCAGACAATCGCTTTTTTCGCCTGATACTCACCGTCAGCCGTTTCAATAGTTACGCCATCATCGTCATGACGAATTGCGGTGACCGGGCAGTTGAACAGTTGCGCACAGCCCGCTTCCTTCGCCAGTTGGATCCAGGTTTTAATCGCCAG‑TTCGCTGCGCAAAAAACCGGAATCAGTTTCAAATAAGCCGATGTAGTTGTCCGGGACGCGTATTTCCGGCCAGCGGGCCATAATCCCTTGCGCATCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTT  >  NC_000913/1119866‑1120355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑