Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,117,707	G→A	G301S (GGT→AGT)	trhO →	tRNA U34 hydroxylase TrhO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,117,707	0	G	A	100.0%	151.9 / NA	49	G301S (GGT→AGT)	trhO	tRNA U34 hydroxylase TrhO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/26);  total (23/26)

AAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCT  >  NC_000913/1117465‑1117947                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 aaGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGAAGGGCTTGCGGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGACGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCGGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTtgttgt                                                                                                                                                                                                                                          <  2:388779/251‑1 (MQ=255)      tGGCATATCGAGGGCGGAATTATTGAATACGCCCTTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCTCATTGCCACCAGTGCGGCGCGCCGTGCGTCAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTTTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTg                                                                                                                                                                                                                                      <  1:458076/250‑1 (MQ=255)         cATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTg                                                                                                                                                                                                                                      >  1:57269/1‑247 (MQ=255)               gAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGCTTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTtgctg                                                                                                                                                                                                                            >  2:456863/1‑251 (MQ=255)                        attattGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAg                                                                                                                                                                                                                   <  1:258242/251‑1 (MQ=255)                                 tACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTAcc                                                                                                                                                                                                          >  1:24581/1‑251 (MQ=255)                                          aaGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCaga                                                                                                                                                                                                    >  1:475990/1‑248 (MQ=255)                                           aGGCGCGCGAGCAGGGCTTGCCTGGGCGTTTTATGGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAgaagaa                                                                                                                                                                                                <  1:231518/251‑1 (MQ=255)                                               gcgcgAGCAGGGCTTGACGGTGCGATTTATTGGCCAAAATTTCGTTTTTGACGAGCGGATGGGCGACCGTATATAGGATGAGATTATCTCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTACGTGTCAAGAATGCGCGGAAAAATACAAAAGATGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGccgagcaaacagc                                                                                                                                                                                            >  2:312081/1‑240 (MQ=255)                                                  cgAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGAc                                                                                                                                                                                         >  1:385829/1‑251 (MQ=255)                                                          gtttGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCTGCTGGGCGATCGTATATCGGATGAGATTATCGCGCATTGCCTCCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTGGCTTTGAAGAAAGCTCGTTACCGCCAGAAGAACAGCGACGCCGTCgg                                                                                                                                                                                 <  1:434697/249‑1 (MQ=255)                                                          gCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACTTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCgg                                                                                                                                                                                 <  1:495884/251‑1 (MQ=255)                                                              gCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCTAACGTATATCGGATGAGATGATCGCGCATTGCCACCAGTGCGGTGCGCCGTGCGACAGCCATACCAACGGTAAAAATTATGGCTTCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTGGTAGTGAGATTTGCTGCGAAGAAATCGCGTTACCGCCAGAATAACAGCGACGCCGTCGGGCa                                                                                                                                                                              <  1:410441/250‑1 (MQ=255)                                                                 ggTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGAc                                                                                                                                                                          >  1:436658/1‑251 (MQ=255)                                                                     ggTTTTATTGGAAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATACCGGATGAGATTACCGCTAATTGCCACCAGGGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGa                                                                                                                                                                      <  2:102349/250‑1 (MQ=255)                                                                        tttATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGaaa                                                                                                                                                                    <  1:441800/250‑1 (MQ=255)                                                                              ggCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGGGAAAATGGCa                                                                                                                                                              >  2:465928/1‑250 (MQ=255)                                                                                               tGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGt                                                                                                                                            >  1:55608/1‑251 (MQ=255)                                                                                                 atgagtggATGGGCAAACGTATATCGGATGAGATTATCTGGCATGTCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATCCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCTCGTTTCCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTCTAATAAGtct                                                                                                                                          <  1:120140/245‑1 (MQ=255)                                                                                                    aGCGGATGGGCGAACGTATATCGGTTGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGGTGTTGTAGTGAGAGTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCgg                                                                                                                                       >  1:165548/1‑250 (MQ=255)                                                                                                           gggCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGCTCTTTAATAAGTCTCGTGGACGTc                                                                                                                                >  2:475937/1‑251 (MQ=255)                                                                                                            ggCGAACGTATAGCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCt                                                                                                                               <  2:414320/251‑1 (MQ=255)                                                                                                              cGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGACCAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTg                                                                                                                              >  2:9919/1‑250 (MQ=255)                                                                                                               gAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATCCAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAGAATGGCAATAAGATCTTTAATAAATCTAGTGGGCGTCTGa                                                                                                                             >  2:488710/1‑250 (MQ=255)                                                                                                               aaaCGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGaa                                                                                                                            <  1:491316/250‑1 (MQ=255)                                                                                                                aaCGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAAt                                                                                                                           >  1:413004/1‑251 (MQ=255)                                                                                                                aaCGTATATCGGATGAGATTATAGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAAt                                                                                                                           <  2:55608/251‑1 (MQ=255)                                                                                                                  cgtagaccGGAGGAGATTATCCCGCATTGCCCCCAGGGCGGCTCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGAGTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATac                                                                                                                         <  2:24581/244‑1 (MQ=255)                                                                                                                                      cgcgCATTGCCACCAGTGCGGGGGGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCGGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATc                                                                                                     <  2:475990/251‑1 (MQ=255)                                                                                                                                       gcgcATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATcc                                                                                                    >  1:238275/1‑251 (MQ=255)                                                                                                                                                    aGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGaataaata                                                                                       <  1:475937/251‑1 (MQ=255)                                                                                                                                                                cGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGAt                                                                           >  1:105459/1‑251 (MQ=255)                                                                                                                                                                        aGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGcc                                                                    >  1:435564/1‑250 (MQ=255)                                                                                                                                                                          ccATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATc                                                                 >  2:413864/1‑251 (MQ=255)                                                                                                                                                                          ccATACCAACTGTAAAAATGATGGCTGCCACCGGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATc                                                                 <  1:53500/251‑1 (MQ=255)                                                                                                                                                                            aTACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTACAGAATGAGAGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTACGAAGAAAGCGCGTTACCGCCGGAAGAACAGCGACGCCGACGGGCAGGACGTGAACATGGCAATAAGATCTTTACTAAGTCACGTGGACGTCTGAATACAAAACTGTGCATTCATGATCCAACAGAATAAATATCATTGACGGAAGCGTGCCATCCg                                                               >  1:292146/1‑251 (MQ=255)                                                                                                                                                                              aCCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCgg                                                              <  1:20497/250‑1 (MQ=255)                                                                                                                                                                                 aaCTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTGGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAAc                                                          <  1:79442/251‑1 (MQ=255)                                                                                                                                                                                     gTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACAttt                                                      <  1:146662/251‑1 (MQ=255)                                                                                                                                                                                         aaaTGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACg                                                  >  1:242988/1‑251 (MQ=255)                                                                                                                                                                                                ggCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACtt                                           >  2:292364/1‑251 (MQ=255)                                                                                                                                                                                                           gctgTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTAc                                  <  1:456863/249‑1 (MQ=255)                                                                                                                                                                                                              gtgtATTCAGTGTCCAGAAGGGTCTGAAAAAGACAAAAGTTGGTGTAGTGTGCTTCGATGCGCAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCAGGCGGGCAGGACGTGAAAATGGCAATAAGATCTTTGATAAGTCGCGTGGACGTCTGAAAACAACACCGTGCATTCCTGATCCCAGAGAATAAATGTCATTGCAGGATGCGTTCCATCCGGCCACCTTTCACGATTACTACTGCTGTACGcttt                             <  2:316322/248‑4 (MQ=255)                                                                                                                                                                                                                   ttCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACt                        >  2:90283/1‑251 (MQ=255)                                                                                                                                                                                                                    tCAGTGTCCAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACt                        <  1:90283/250‑1 (MQ=255)                                                                                                                                                                                                                           ccAGTATGCGCGGAAAAATACAAAAGTTGTTGTAGGGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACAGTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAaataat                <  1:229454/251‑1 (MQ=255)                                                                                                                                                                                                                             aGTATGCGCGGAAAAATACAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATc               >  2:106980/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggAAAAATATAAAAGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACtt     <  1:39657/251‑1 (MQ=255)                                                                                                                                                                                                                                         aaaaTACAAAAGGTGTTGTAGTGAGATGGGCGGGGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCAGTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCt  <  1:453792/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 AAGTCTGGCATATCGAGGGCGGAATTATTGAATACGCCCGTAAGGCGCGCGAGCAGGGCTTGCCGGTGCGTTTTATTGGCAAAAATTTTGTTTTTGACGAGCGGATGGGCGAACGTATATCGGATGAGATTATCGCGCATTGCCACCAGTGCGGCGCGCCGTGCGACAGCCATACCAACTGTAAAAATGATGGCTGCCACCTGCTGTTTATTCAGTGTCCAGTATGCGCGGAAAAATACAAAGGTTGTTGTAGTGAGATTTGCTGCGAAGAAAGCGCGTTACCGCCAGAAGAACAGCGACGCCGTCGGGCAGGACGTGAAAATGGCAATAAGATCTTTAATAAGTCTCGTGGACGTCTGAATACAACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCT  >  NC_000913/1117465‑1117947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑