Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,421	C→T	S520F (TCT→TTT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,421	0	C	T	100.0%	120.1 / NA	37	S520F (TCT→TTT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/20);  total (17/20)

ATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGG  >  NC_000913/1112205‑1112665                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     aTGATTTCGTGGAAGCGGCGTTGATGCGCCGAGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTc                                                                                                                                                                                                                    >  1:317875/1‑251 (MQ=255)                         tGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGAGCTCTCTACTGCATTGCAGGt                                                                                                                                                                                             >  1:475335/1‑250 (MQ=255)                                                    tttcttatgatcccccggttatgtATGAAGTTTTGCCGCCTATCTTGCATGGTGAGCTAACACGGTG‑CCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGTTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTTTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATa                                                                                                                                                                  <  1:271706/227‑1 (MQ=255)                                                                     ggTTCTTATGAAGAATTACCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCACTGTGGTTTCTGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACAGCAATACTTCCTGCAACCCCGGc                                                                                                                                                 >  2:338810/1‑250 (MQ=255)                                                                      ttttttatgaaaatgtGCCGCCTAACTTGCTGAATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCa                                                                                                                                                <  1:355282/235‑1 (MQ=255)                                                                             tGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTcc                                                                                                                                        >  1:425116/1‑251 (MQ=255)                                                                                     cgccgaccttcctggttgATGATCTAAATCC‑AGACCGGCGATGGTGCCACGGTAACCTGAGTAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCTCCGTGCGGTGTTCCTGACGGTCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGtgtg                                                                                                                                 <  1:276233/235‑1 (MQ=255)                                                                                             aaCTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTg                                                                                                                        >  1:44032/1‑251 (MQ=255)                                                                                                  gcttgAGGAGCAAAACCG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTc                                                                                                                   <  1:194654/251‑1 (MQ=255)                                                                                                        agaagcaaaaccgggACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAg                                                                                                              <  1:403384/237‑1 (MQ=255)                                                                                                             tAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCg                                                                                                        >  2:37353/1‑251 (MQ=255)                                                                                                                      gACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGGCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCAc                                                                                                 <  1:20624/250‑1 (MQ=255)                                                                                                                       cccgccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACtt                                                                                               <  1:265904/250‑1 (MQ=255)                                                                                                                           ccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAAcc                                                                                                                                                                        >  2:223780/1‑174 (MQ=255)                                                                                                                           ccgATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAAcc                                                                                                                                                                        <  1:223780/174‑1 (MQ=255)                                                                                                                                      ccGGTAACCTGATGAACTGCCGTCTGTTCCTGGTGAAGGTGATGCACCCGGTTGTCCGGGCGGTGTTCCTGACGGGCGTGATGTTTGATCTCTCCGCTCCGCTGTGGTTTATGTACCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTGCCTGCAACCACGGCAGTTATTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGAGTGCACTTGTTGCTTCGACCATg                                                                                <  2:48881/250‑1 (MQ=255)                                                                                                                                        ggTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGt                                                                              <  1:37353/251‑1 (MQ=255)                                                                                                                                        ggTAACCTGAAGTACTCCCGTCTGTTCCTGGGGAAGGTTATTCACCCGGTTCCCCGTGCGGTGTTCCTGACGGGCGGGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCACTGCAGGTAGTGCATGCGTTGACCGAACCGCAATTCTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGt                                                                              <  2:139538/251‑1 (MQ=255)                                                                                                                                             ccTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCtgt                                                                         >  2:243494/1‑251 (MQ=255)                                                                                                                                                    aaCTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCt                                                                  >  1:226203/1‑251 (MQ=255)                                                                                                                                                        tCCGTCTGTTCCTGGTGAAGGGTATGCTCCCGGTTCACCGTGCGGTGTTCCTGTCGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTTGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGc                                                                <  1:311140/249‑1 (MQ=255)                                                                                                                                                        tCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGCGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCATGCCg                                                              >  1:28555/1‑251 (MQ=255)                                                                                                                                                        tCCGTCTGTGCCTGGTGAAGGGTATGCCCCCGGTTCCCCGGGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGcc                                                               <  2:41612/250‑1 (MQ=255)                                                                                                                                                              cgtgcctgttggatgttattcACCCGGTTCGCCATGCTGTGTTCCGTACGGGCGTGATTTTGTCTCTTTTCTCTCCGCTGTGGGTTATGTTCCTCGCGCTCTCTCCGGCACTGCAGGTAGTGCATGGGTCGACCGACCCGCAATACTTCCTGCAACCACGGCAGTTGTTCTCAGTGTGGCCGCAGGGGCGTTCTGAGCTGGCGATTGCACTTTTTGCTTCGACCACTGTGCTGTTGTTCCTGCCGAAGtt                                                         <  2:391618/230‑1 (MQ=255)                                                                                                                                                               gTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGtt                                                         <  1:129438/249‑1 (MQ=255)                                                                                                                                                               gTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTAt                                                       >  1:191981/1‑251 (MQ=255)                                                                                                                                                                                 gCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCt                                     <  1:465331/251‑1 (MQ=255)                                                                                                                                                                                           ccccGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAg                            <  1:104320/248‑1 (MQ=255)                                                                                                                                                                                           cACCGTTCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCCCCGCTGTGGTTTAGGTTTCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTGCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTTCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTTAGCATTTTGCTTATCTCGTGCAAAg                            <  1:314448/250‑1 (MQ=255)                                                                                                                                                                                           cACCGTGCGGTGTTCCTGACGGGCGTGATTTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTTCAAAg                            <  1:77181/250‑1 (MQ=255)                                                                                                                                                                                             ccGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGaa                         <  1:448812/251‑1 (MQ=255)                                                                                                                                                                                             ccGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGa                          >  1:327400/1‑250 (MQ=255)                                                                                                                                                                                                     tgtTCCTGCCGGGCGTGATTTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTCCTGCATTTCGGGTAGTGCATGCGTTGACAGAACCGCAATCCTTCCTGCATCCACGGCAGTTGTTCCCAGGGTGGCCGAAGTGGCGTCCTGCGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTTTTCCTGCCGCAGTTATTCAGCGTTTTGCTTATCTGATGCCAAGGAGCGactgac                 >  2:200753/1‑246 (MQ=255)                                                                                                                                                                                                       ttCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAAt                >  2:210280/1‑250 (MQ=255)                                                                                                                                                                                                             aCGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATAcggcgg         >  1:49700/1‑251 (MQ=255)                                                                                                                                                                                                                   gTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTg   >  1:30021/1‑251 (MQ=255)                                                                                                                                                                                                                    tGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTgg  >  1:208591/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     ATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACG‑TGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTCGCGCTCTCTACTGCATTGCAGGTAGTGCATGCGTTGACCGAACCGCAATACTTCCTGCAACCACGGCAGTTGTTCCCAGTGTGGCCGCAGTGGCGTCCTGAGCTGGCGATTGCACTTTTTGCTTCGACCATGGTGCTGTTGTTCCTGCCGAAGTTATTGAGCATTTTGCTTATCTGGTGCAAAGGAACGAAAGAATACGGCGGCTTCTGG  >  NC_000913/1112205‑1112665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑