Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,112,209	C→T	D449D (GAC→GAT)	opgH →	osmoregulated periplasmic glucans biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,112,209	0	C	T	100.0%	123.7 / NA	39	D449D (GAC→GAT)	opgH	osmoregulated periplasmic glucans biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/16);  total (23/16)

CGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTC  >  NC_000913/1111962‑1112455                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       cGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTc                                                                                                                                                                                                                                                     >  2:223178/1‑251 (MQ=255) cGAACGCCGGGACCATGCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTc                                                                                                                                                                                                                                                     <  2:307281/251‑1 (MQ=255)            aTCATTCAGTCGTCGCCGAAAGCGTCCGGAACGGATCCGCTGTATGCGCGCGTTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTAGAGCCGGTTTGCACTTCTGGCAAATTGGCGAGTCGAACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTGCGCTGCCGTGCGAAGGTTCCTGTGCCGGTTCAAGCC‑TGTCACATGATTTCGTGGAAgcggc                                                                                                                                                                                                                                          <  1:180014/251‑1 (MQ=255)               atgcaggcgtcgCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGtt                                                                                                                                                                                                                                       <  1:356451/244‑1 (MQ=255)                          gCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTg                                                                                                                                                                                                                            >  1:259281/1‑251 (MQ=255)                            cGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCa                                                                                                                                                                                                                          <  1:483259/251‑1 (MQ=255)                                        gTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGTGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGGTGCGCCGTGCAGGTTGGGGGGtt                                                                                                                                                                                                              >  2:377747/1‑250 (MQ=255)                                           gggATACGCTGTATGCGCGCTGTCGGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTgg                                                                                                                                                                                                           <  2:357332/250‑1 (MQ=255)                                            ggATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTgg                                                                                                                                                                                                           <  1:249160/250‑1 (MQ=255)                                                    ggTATGCGCGCTGGCAGCAGTGCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTAc                                                                                                                                                                                                  <  2:189566/250‑1 (MQ=255)                                                         gcgcgcTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGAtc                                                                                                                                                                                              <  1:223178/250‑1 (MQ=255)                                                                   agcagTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGACGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGGTTGCTTACGATCTCCCGGGTTCt                                                                                                                                                                                   >  2:129438/1‑251 (MQ=255)                                                                     cagTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTa                                                                                                                                                                                 <  1:453055/251‑1 (MQ=255)                                                                                ccGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTgc                                                                                                                                                                      <  1:302504/251‑1 (MQ=255)                                                                                     tgtATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCt                                                                                                                                                                 >  2:274276/1‑251 (MQ=255)                                                                                      gtATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCt                                                                                                                                                                 <  1:341619/250‑1 (MQ=255)                                                                                      gtATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCt                                                                                                                                                                 >  1:41612/1‑250 (MQ=255)                                                                                          gggacACTGTTTCCAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGTTGGCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAActt                                                                                                                                                            <  2:461813/247‑1 (MQ=255)                                                                                          gggCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGCTTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGGTACTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCGGGTTGGGGGGTCTGGTTTGCTTACGATATCCCGGGTTCTTATGAAGAATTgccgccacactt                                                                                                                                                            >  2:298597/1‑245 (MQ=255)                                                                                            gCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTAGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAActtgc                                                                                                                                                          >  2:448812/1‑251 (MQ=255)                                                                                                  gTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTtgatg                                                                                                                                                    >  2:116047/1‑251 (MQ=255)                                                                                                      aCAGCCGGTTTGCACTTCTGGCAACTTTGCGAGTCGCTCTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGc                                                                                                                                                 <  1:434114/250‑1 (MQ=255)                                                                                                      aCAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGc                                                                                                                                                 <  1:283086/250‑1 (MQ=255)                                                                                                                  cACTTCTGGCAACTTGGCGATTCGCACTACTGGGGACCTAACGCGATTCTCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGATCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTAAGATCTCACGGGTTCTTATGAAGAATTCCCGCCTAACTTGCTTGATGCGCTAAAACgggaacg                                                                                                                                    >  2:20624/1‑245 (MQ=255)                                                                                                                    cTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGAccgcc                                                                                                                                  >  2:465331/1‑251 (MQ=255)                                                                                                                        tGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGACGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATg                                                                                                                              >  1:159002/1‑251 (MQ=255)                                                                                                                                          cACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCtgat                                                                                                            >  1:48881/1‑251 (MQ=255)                                                                                                                                              cctGGGGAAATAACGCGTTTACCCGGGTGAAACCGTTTAGCGAGCTCTGGCCCCTGGCCCCGCGGCCGGGGGAGGTTTCCTTTGCCG‑TTCAATCCCTGTCACTTGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTGGGGGGGTTTGGATTGCTTACGACCCCCGGGGTTCTTATGAAGAATGGCCGCCTACCTTGCTTGAGGAGCAAAACCGTGACCGCCGATGGTGCCACGGTAACCTGATGAAc                                                                                                        <  1:274276/250‑1 (MQ=255)                                                                                                                                                          aCGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTc                                                                                            >  1:263329/1‑251 (MQ=255)                                                                                                                                                                             aCCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCAcc                                                                         >  2:311140/1‑251 (MQ=255)                                                                                                                                                                              ccGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCAccc                                                                        >  1:505326/1‑251 (MQ=255)                                                                                                                                                                               cGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACCTGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGGAGGGCATGCACCCg                                                                       >  1:326112/1‑251 (MQ=255)                                                                                                                                                                                gTTTAGCGAGAACTGCGCGCGGACTCCGCGTCCGGGCGAAGGTTCCTTTGCCGGTTCAATCA‑TGTCACATGTTTTAGTGGAAGGGGCGTTTCTGCGCAGTGCACGTTGGGGTGTCTGGCTTGCTTGCGGTCTCCCGGGTTCTTATGAAGAATTGCAGCCTAACTTGCTTGATGTGCTAACACGTGACCGCGGAAGGTGCCACGGTAACCTGGGGAGCTTCCGTCTATCCCTGGTGacggacatgcacacgg                                                                      >  2:66307/1‑236 (MQ=255)                                                                                                                                                                                                gcACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGCACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGGGCGgtttt                                                      >  2:265904/1‑248 (MQ=255)                                                                                                                                                                                                  aCTGGCTCCGCTGCCGAGCGATGGATCCTTAGCCGGCTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTCATTATGAAGAATTGCCGACTAACTTGCTTGATGAGCTAAACCTGGCCCGCCGATGGCGCCTCGGTAACCTGATGTACTTCCGTATGTTCCTGGTGAAGGGTCTGTACCCGGTTCACCggccggggttcc                                                    >  2:340818/1‑240 (MQ=255)                                                                                                                                                                                                        cccGCTGCCGGGCGAAGGTTCCTTTGCGGTTTCAAGCT‑TGCCAGATGATTTCGGGGAGGCGGCGTTGATGCGCCGTGCAGGTGGGGGGGTCTGGATGCCTAACGATCCCCCGGGTTCTTATAAAGAATTGCCGCCTAACTTGCTTGAGGAGTTAAACCGGGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACgg                                              <  1:116047/250‑1 (MQ=255)                                                                                                                                                                                                             tGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGCTTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGATGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTAACCGTGCGGTGTTCCTGACGGGCGTg                                         >  2:193092/1‑251 (MQ=255)                                                                                                                                                                                                                        ggTTCCTTTGCCGGTTCAATCC‑TGTCACATGATTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCCCCGTGCGGTGTTCCTGACGGGCGTGATGttttctct                              >  2:194654/1‑244 (MQ=255)                                                                                                                                                                                                                                                     aTGATTTCGTGGAAGCGGCGTTGATGCGCCGAGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTTTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTc  >  1:317875/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGCGTGTATGGGCCACTGTTTACAGCCGGTTTGCACTTCTGGCAACTTGGCGAGTCGCACTACTGGGGACATAACGCGATTATCCGCGTGAAACCGTTTATCGAGCACTGCGCACTGGCTCCGCTGCCGGGCGAAGGTTCCTTTGCCGGTTCAATCC‑TGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTATGTTCCTC  >  NC_000913/1111962‑1112455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑