Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,740	C→T	L136F (CTT→TTT)	opgG →	osmoregulated periplasmic glucans biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,740	0	C	T	96.3%	73.0 / ‑5.4	28	L136F (CTT→TTT)	opgG	osmoregulated periplasmic glucans biosynthesis protein G
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (13/13);  minor base G (0/1);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

TATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGT  >  NC_000913/1109493‑1109982                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   tatGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGAAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGCTACCCCGGTCAAAATACCTGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCAGCGATGTTCAGCATGACACAGATACGGTAAGAGACCGTGGCTTTTCCGGTTTTCAAGTGtttt                                                                                                                                                                                                                                                 >  1:21/1‑251 (MQ=255)           gcgGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCATCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCa                                                                                                                                                                                                                                        >  1:142060/1‑250 (MQ=255)               aCTATCAGCAGATCCAGTTTAATCATGACAAAGCGTTCTGAAACAATCTGAAGACCCCATTCAAACTCGAGTTCTTCCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAATTGACTTCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAATACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGc                                                                                                                                                                                                                                   <  1:375368/251‑1 (MQ=255)                cTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTAATTCGATACCCTGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGGTTTAAAGCGTTTTACCCGATCAACatc                                                                                                                                                                                                                                   >  1:438624/1‑248 (MQ=255)                     agcagATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGAt                                                                                                                                                                                                                             <  1:35909/251‑1 (MQ=255)                        agATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATACAAACTCGAGTTCTACCATCAGGTTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAAGCAAATACAGCCCGGATTATTTCTCTTGCGGCGATGGTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATaaa                                                                                                                                                                                                                          <  1:302558/251‑1 (MQ=255)                              agtatAATCATGACAAAGCGTACTGGAACAATCTGAATACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTGGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGAt                                                                                                                                                                                                                    <  1:367023/247‑1 (MQ=255)                                       aTGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGACAAAATAAATTAATTGACTGCCAACGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAGAGGAACTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAATGATAAAAACGATGAAATCGTc                                                                                                                                                                                                           >  2:239455/1‑251 (MQ=255)                                                                      ccctttCAAATTCGAGGTCTACCATCAGGGTATGTACTTCGATACCCCGATCAAAAGATATGAAGTTACGGCCACCGCAGTCAAACGAATCAAATCCATCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGGAAAATACCTTGGCTTTGCCGGGTTTAAAGTGTTTTTCCCGATCAACAGCAAAGATAAAAACTATGAAATCGTCATCTAGCTCGGGGCCAGCGATTGCCGCGTGa                                                                                                                                                                            <  2:313204/247‑1 (MQ=255)                                                                                                                           aaacaaatGAAGTGACTGCCTCCGCCGTCAAACGAATCAAAAACAGCCCGGATTATGTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAAGCGGCAGCATGCCCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGAttcc                                                                                                                       <  2:269821/247‑4 (MQ=255)                                                                                                                            aataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCg                                                                                                                      <  1:116176/251‑1 (MQ=255)                                                                                                                             ataaatGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGc                                                                                                                     >  2:138181/1‑251 (MQ=255)                                                                                                                                     aGTGACTGCCCCCGCCGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCAt                                                                                                             <  2:118329/251‑1 (MQ=255)                                                                                                                                                     gacAAACGAATCAAATACAGCCCGGATTATTTCACTTGCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTcc                                                                                             <  2:98765/249‑1 (MQ=255)                                                                                                                                                          aCGAATCAAATACAGCCCGGATTCTTTCACTTTCGGCGATGTTCAGCATGACAAAGCTACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTAACCGAACAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTCTTTCCGCGGGATTGGTGCAGGTCCGGTTTATGGCCTTGCTGCCCGCGGCCTGGCACTTGATACCGCCTTGCCATCGGGTGAAGACTTTCCGCGCt                                                                                        >  2:49393/1‑251 (MQ=255)                                                                                                                                                            gAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGCCAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCCGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTCTGGCCTTTCTGCCCGCGGCCTGGCAATTGCTACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTc                                                                                      >  1:27577/1‑251 (MQ=255)                                                                                                                                                                  aaTACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAag                                                                                  >  1:232131/1‑249 (MQ=255)                                                                                                                                                                            ggATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGGGCAGGTCAGGATTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGAGCTTCAAAGAGTTATGGATCg                                                                      >  1:323538/1‑251 (MQ=255)                                                                                                                                                                                       cTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGGGCGTCCaaa                                                            >  2:194916/1‑250 (MQ=255)                                                                                                                                                                                                  gTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATa                                                 <  1:287676/250‑1 (MQ=255)                                                                                                                                                                                                   ttCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATaa                                                >  1:60694/1‑250 (MQ=255)                                                                                                                                                                                                                    aCGGTAAAAGACCTTGGCTTTGTCGGATTTAAAGTTGTTTACCCTATCAACAGCAATGATAAAAACGATGATATTGTCATCATGCTCGGGGCCAGCTATTTCCGCGTGTTTGGTACATGTCAGGTTTATTGACTTTCTGCCCGCGGCCTGGCACTTGATACCGCCTTGCCACCACGTGAAGAATTTCCTCACTTCAAAAAGTTCTTGAACGATCTTCCAAAACCGACTGATAAACGTTTAACCATCCATg                               <  2:134424/250‑1 (MQ=255)                                                                                                                                                                                                                    aCGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGc                              <  2:396248/251‑1 (MQ=255)                                                                                                                                                                                                                     cGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACAGACTGATAAACGTTTAACCATCTATGc                              >  2:10577/1‑250 (MQ=255)                                                                                                                                                                                                                          aaaGACCTTGGCTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCt                        >  1:474550/1‑251 (MQ=255)                                                                                                                                                                                                                           aagacttgggcttgtccGGTTTTAAAGTGTTTGACCCGATCAACAGCAAAGATAAAAACGATTAAATCGTCAGCATGCTTGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTTAGGTTTATGGTCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGTTGAAGAATTGCCGCGCTTCAAAGAGTTCTTGTGCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCtt                       <  2:160307/236‑1 (MQ=255)                                                                                                                                                                                                                               ccgtGGCTTTGACTGTATTCAAGTGTTTTACCCGATCAAAAGAAAAGATAAAAACGATGAAATCGTCAGCTTGCTCGGGGCCGGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCTTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGACATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCAAGGGTCCAAAACCGACAGATACACGTTTAACCATCACAGCATTGCATGACt                   >  2:450531/4‑251 (MQ=255)                                                                                                                                                                                                                                     cTTTGCCGGTTTTAAAGTGTTTTACCCGATCAACAGCAACGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGAGGCCTGGCAATTGATACAGCCTTGCCATCGGGTGAAGAATTTCCTCGCTTAAACGAGTTCTGGATCGTGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCCTTGCTTGACTcgccgc             >  1:42125/1‑251 (MQ=255)                                                                                                                                                                                                                                                 tAAAGTGTTTTGCCCGATCAACACGAAATCTAAAAACAATGAAACCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGTGGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTTTCAATTGATACCGCCTTGCCATCGGTTGAAGAATTTCCGCGCTTCATAGTGTGCTTGAGCGAGCGTCCAAACCCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGGCAggg  <  2:297523/250‑2 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   TATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTTACCCGATCAACAGCAAAGATAAAAACGATGAAATCGTCAGCATGCTCGGGGCCAGCTATTTCCGCGTGATTGGTGCAGGTCAGGTTTATGGCCTTTCTGCCCGCGGCCTGGCAATTGATACCGCCTTGCCATCGGGTGAAGAATTTCCGCGCTTCAAAGAGTTCTGGATCGAGCGTCCAAAACCGACTGATAAACGTTTAACCATCTATGCATTGCTTGACTCGCCGCGTGCGACAGGT  >  NC_000913/1109493‑1109982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑