Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,090,508	C→T	E594E (GAG→GAA)	pgaA ←	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,090,508	0	C	T	100.0%	46.9 / NA	16	E594E (GAG→GAA)	pgaA	partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export outer membrane porin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/2);  total (14/2)

GTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGA  >  NC_000913/1090268‑1090742                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           gTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCAAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGCTTGAAAACGCGTTCAGCGTATTCTGCCTCg                                                                                                                                                                                                                                   >  1:434490/1‑250 (MQ=255)                                gagaCACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACAcc                                                                                                                                                                                                   >  1:419363/1‑251 (MQ=255)                                       cGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCAAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGCGCCAGTTATTAAGTGCACGCCACTCAACACCCGCAAGc                                                                                                                                                                                            >  2:126568/1‑251 (MQ=255)                                          aCTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTTCCTGTAACTCCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGTGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGcc                                                                                                                                                                                           >  2:45850/1‑249 (MQ=255)                                               cgccgcTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGGTTATGCTCATGATTGAACACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAAAACCCGCAAGCCAGTcgcg                                                                                                                                                                                    >  2:108190/1‑251 (MQ=255)                                                                                                  acacCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGc                                                                                                                                 >  2:90349/1‑251 (MQ=255)                                                                                                    acCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAAACCCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATACCCGAATCCAGCa                                                                                                                                >  2:458301/1‑250 (MQ=255)                                                                                                                                     ggagagGCGTTCCAGTTGCGAACCAAAACGATAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGAGACGGGATTATGCTCATGATTGCAAACGCGTTCAGCGTATTCTGACTCGAGACAGATATTACTTGACCGCCACTCACAACCCGCAAGCGAGTCGCGAACAATCCCTTTTACTTCGCTAAATTGTCCATAGGAATAACATCATCCAGCAAAAACGCGCCAATCATCTTTCATCGGTGGTGAAt                                                                                              >  2:317579/2‑251 (MQ=255)                                                                                                                                               tCCAGTTGCGAACCAATACGCCAGATATCATTAAAATCATACCAGCCAGACAGGCGCGAGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGAtggg                                                                                    >  1:445257/1‑250 (MQ=255)                                                                                                                                                                   ccAGTTATCATTAAAATCATACAAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCgcaatcctttt                                                                >  2:295616/1‑241 (MQ=255)                                                                                                                                                                       ttATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTAcc                                                            >  1:217010/1‑251 (MQ=255)                                                                                                                                                                              aaaaaTCATACCAGCCAGCCGGCCGCGCCCCGGGTTTTTCCTCATGATTAAAACCGCGTTCAGCGTTTCCTCCCCCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATc                                                      <  1:458301/249‑1 (MQ=255)                                                                                                                                                                                   tCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCCTGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGcc                                                >  1:140565/1‑251 (MQ=255)                                                                                                                                                                                       aCCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTcc                                            >  2:329716/1‑250 (MQ=255)                                                                                                                                                                                                                         aTGATTGAAAACGCGTTCAGCGTATTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGct          >  1:131740/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaaCGCTTCCAGCGTATCCTGCCGCGAGCCAGATATTCCGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGa  <  1:184950/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           GTTACTGTCGGAAAAATCAGTGAAAGCCCAGGAGACACCGTACTTACGCCGCTCATTTTGATACCAGCGAACATAAGCCTGAGCACTGTTGCCTGTAACACCATTTTTCATTGCCCGTAATGGAACGCGGTGAGAGAGGCGTTCCAGTTGCGAACCAATACGCCAGTTATCATTAAAATCATACCAGCCAGACAGGCGCGCGCCGGGTTTATGCTCATGATTGAAAACGCGTTCAGCGTACTCTGCCTCGAGCCAGATATTACGTGACCGCCACTCAACACCCGCAAGCCAGTCGCGAACAATCCCTTTTCCTTCGCTAAATTGTCCATCGGCATAACCGAATCCAGCAAAACCGCGCCAGTTATCCTTCAGCGGTGGTGAATAAACGATGGTGGTTAAGTCGACATCATGTTTACCACTATCCGGGCCTTCGGCATCAATTCCTGTTGAGCCAGCGATACGAAGCTCTGCAAGA  >  NC_000913/1090268‑1090742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑