Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,078,268	A→T	F205L (TTT→TTA)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,268	0	A	T	100.0%	145.2 / NA	47	F205L (TTT→TTA)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/27);  total (20/27)

AAGAGTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGT  >  NC_000913/1078033‑1078509                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  agtaggaaaggataCCTTTCTCGTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTa                                                                                                                                                                                                                                    <  2:450184/238‑1 (MQ=255)  agagTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGGAACCAGTTTGCCAGTACACAGCAGCCCCCAGGTGGCGGCATTAAATAACAGTGGCGGGCTAc                                                                                                                                                                                                                                   >  2:480535/1‑251 (MQ=255)           gaatccTTTCTCTTCCAGCGTGCGGGCATTGGCTAACGTGTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAAtgt                                                                                                                                                                                                                          <  2:432035/247‑1 (MQ=255)           gAAACCTTTCGCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGGGACGAACCGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAAtgt                                                                                                                                                                                                                          <  2:287985/251‑1 (MQ=255)                        tCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCa                                                                                                                                                                                                                <  1:454349/248‑1 (MQ=255)                         ccAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGtt                                                                                                                                                                                                             <  1:309747/250‑1 (MQ=255)                                 cGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCt                                                                                                                                                                                                    <  1:135244/251‑1 (MQ=255)                                         ggCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGt                                                                                                                                                                                            >  1:460472/1‑251 (MQ=255)                                         ggCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGt                                                                                                                                                                                            <  1:396695/251‑1 (MQ=255)                                                   gccGCGATGGTTTCGCAAGGGACGAACTGCTCACCTATTAGGCGCCACGCCATATCCACACCTGTGCGGATCAGCGGTTCACCGCGGTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGATGGCTGCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGCGGCGGCATTAACTAACAGTGACGGGATACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAAtt                                                                                                                                                                                    <  2:111484/248‑1 (MQ=255)                                                       cgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGGTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAAcgcg                                                                                                                                                                              <  1:318/251‑1 (MQ=255)                                                           ggTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTcgc                                                                                                                                                                          >  1:175656/1‑251 (MQ=255)                                                               tCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCggg                                                                                                                                                                       <  1:107351/250‑1 (MQ=255)                                                                    aGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGCCGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGCATTAACGCCTCGCGGGTGGCtt                                                                                                                                                                 >  2:152337/1‑251 (MQ=255)                                                                               gCTCACCCAGCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCTGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATa                                                                                                                                                      <  1:58893/251‑1 (MQ=255)                                                                                           ggCGCATCGCCATAGCCACACCTTTGCGGTTCAGCGGTTCACCTCTTGTACCGATAATGCGGTTCAGCGGGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCt                                                                                                                                          <  2:464969/251‑1 (MQ=255)                                                                                              gcATCGCCATATCCACACCTGTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTcc                                                                                                                                       <  1:484303/251‑1 (MQ=255)                                                                                               cATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCg                                                                                                                                      <  1:105239/251‑1 (MQ=255)                                                                                                    ccATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAAGACGCCACAACGCTTCCGcc                                                                                                                                    >  1:482438/1‑248 (MQ=255)                                                                                                                      ggATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCt                                                                                                               >  1:91377/1‑251 (MQ=255)                                                                                                                          cAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCt                                                                                                           <  1:152337/251‑1 (MQ=255)                                                                                                                          cAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAAATAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACAACTCAGCGCCACGCCTTCCt                                                                                                           >  2:372424/1‑251 (MQ=255)                                                                                                                          cAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGACGAGGCTGGCTTCGTTATGGGTGGTAACCAGTTTGCCAGTCAACAGCAGCCCCCAGGTTGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGTTTTTGTCGCGAATTCACGCGTCGCGGGTGGCTTTGTCGGGAATTCGCAACAACGCTTACGCCTGACACCTCAGCGCCCCGCCTTCCt                                                                                                           >  2:433482/1‑251 (MQ=255)                                                                                                                               gTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGAt                                                                                                      <  1:217167/251‑1 (MQ=255)                                                                                                                                  cACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGa                                                                                                    <  2:121089/250‑1 (MQ=255)                                                                                                                                            aCCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTcg                                                                                                                                                                           <  2:111268/169‑1 (MQ=255)                                                                                                                                            aCCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGATTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTcg                                                                                                                                                                           >  1:111268/1‑169 (MQ=255)                                                                                                                                                   aTGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGCGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGagatcggaagagc                                                                                  <  1:114915/251‑13 (MQ=255)                                                                                                                                                         ttCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAAccc                                                                            >  1:243954/1‑251 (MQ=255)                                                                                                                                                          tCAGCGAGCGGGAGAGGCTGGCTGCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAAccc                                                                            <  1:80859/250‑1 (MQ=255)                                                                                                                                                            agcgagcgGTAAGGGCTGGCTGAGTTATGGGTGGAAACAAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTGGCTTATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCTCCAGACACATCAGCGCCACGCCTTCCGGCGATGACAGCGAAAACTCCTGCAATACCCCCTg                                                                         <  2:124860/251‑1 (MQ=255)                                                                                                                                                               gagcgGGAGAGGCTTGCTGCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                      <  1:312815/251‑1 (MQ=255)                                                                                                                                                                agcgGGAGCGGCTGGCTTTGTTATGGGAGGAACCCCGTTTGCCAGGATACAGCAACCCCCAGGTGGCGGCATTACCTAAAAGTGACAGGCTACGACCAATGTGTGACTGCCAGTTACCGTTACTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTATCGGGACTAAGAACCAAAGCTTCCGCCCGACACATCAGCGCCACGCCTTCCTGGGCTGACAGCGAAAACTCCTGCAATAAaccccggcgc                                                                     >  2:313444/1‑245 (MQ=255)                                                                                                                                                                   gggAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCAt                                                                   >  2:152485/1‑250 (MQ=255)                                                                                                                                                                      agagGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGGGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGAAAGCGAAAACTCCTGCATTAACCCCTGGACCATACCt                                                               >  2:140095/1‑251 (MQ=255)                                                                                                                                                                          gctggctTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTgcgc                                                           <  1:480535/251‑1 (MQ=255)                                                                                                                                                                                              aaaCCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGGCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCAATGGCATTTTTTTg                                        >  1:363767/1‑250 (MQ=255)                                                                                                                                                                                                 ccAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGAtt                                     >  1:136213/1‑250 (MQ=255)                                                                                                                                                                                                    gttggCCAGTAAACAGCAGCACCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGACTTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACCCATAAGCGCCACGCCTTCCTGCGATGACAGCGAAACCTCCTGCAATAACCCCTGGACCATCCCTGCGCGAACACTGGCATTTTTTTGATTACGc                                 >  2:258907/5‑251 (MQ=255)                                                                                                                                                                                                           aGTAAACAGCATCCCCCAGTGGGCGGCATTAACTAACAGTGACGGGCGACGACCAATGTGTGGCTGCCAGTTACCGTTGTTGATTTTGTCGCGAAGTAACGCTTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCTCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCTCTGGCATTTTTTTGATTACGCAGTTTAt                          <  2:498664/251‑1 (MQ=255)                                                                                                                                                                                                           aGTAAACAGCAGCCCCCAGGGGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTAt                          <  2:124966/251‑1 (MQ=255)                                                                                                                                                                                                                     agcCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGa                                                                       <  2:88346/196‑1 (MQ=255)                                                                                                                                                                                                                     agcCCCCAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGa                                                                       >  1:88346/1‑196 (MQ=255)                                                                                                                                                                                                                        ccccAGGTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTggt              >  2:3450/1‑248 (MQ=255)                                                                                                                                                                                                                         cccgggTGGCGGCTTTAACTAACAGTGACGGGCTTCGACCAATGGGGGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTACCGCGTCGCGGTTGGCTTTGTCGGTAATACGCAACAACGCTTCCTCCAGACACATCAGCGCCACGCCTGCCTTCGAGTACAGCGAAAACTCCTGCAACAACCCCTGGACCATACCTGCGCGACCACTGGCAGTTTTTTGATGACGCAGTTTATCGGCCAGCTGATAc            <  2:315784/247‑1 (MQ=255)                                                                                                                                                                                                                              gTGGCGGCATTAACTAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCCGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCCTTTTTTTGATTCAGCAGTTTATCGGCCAGCTGATACGcc         >  1:406097/1‑249 (MQ=255)                                                                                                                                                                                                                                   ggcATTAACTAACAGTGCCGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGCCACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACCCCAGTTtgt  >  2:497200/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  AAGAGTAACGGAAACCTTTCTCTTCCAGCTTGCGGGCATTGGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGT  >  NC_000913/1078033‑1078509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑