Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,699	C→T	A372V (GCC→GTC)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,699	0	C	T	98.1%	156.9 / NA	55	A372V (GCC→GTC)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (20/32);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACT  >  NC_000913/1066464‑1066939                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 gCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCt                                                                                                                                                                                                                                   >  1:140228/1‑251 (MQ=255)    ctGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGc                                                                                                                                                                                                                                 >  2:391822/1‑250 (MQ=255)                           aCCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGc                                                                                                                                                                                                         >  1:370520/1‑251 (MQ=255)                                       agggttggttgGGCACGACTCCAACATTGCCTCTCTGTTACCTGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTTTCAGAGTGCGGCACAGTTACGTAATTACGCTGCGTTaa                                                                                                                                                                                                                                       >  2:367849/4‑209 (MQ=255)                                       aGTGTTGGTTGGGCACGACACCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTaa                                                                                                                                                                                                                                       <  1:367849/209‑1 (MQ=255)                                              gttggGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGccc                                                                                                                                                                                      <  1:341561/251‑1 (MQ=255)                                                   gCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCACTCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGAACCTGCGCAGCGTGTGAAGCTGGAAtagatcggaagagcggcgg                                                                                                                                                                                 >  2:367349/1‑233 (MQ=255)                                                   gCACGACTCCAACATTGCCTCTCGGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTACCCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAAt                                                                                                                                                                                                   <  1:367349/233‑1 (MQ=255)                                                       gACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACATCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCATGTGACTCTGGAATTAAGCGGTTGCCCGATAGACGc                                                                                                                                                                             >  2:184772/1‑251 (MQ=255)                                                          tCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTgg                                                                                                                                                                          >  2:29070/1‑250 (MQ=255)                                                            cAACATTGCCTCCCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATg                                                                                                                                                                        <  2:369652/251‑1 (MQ=255)                                                               cATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGtt                                                                                                                                                                     <  2:140228/251‑1 (MQ=255)                                                                         ctGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGccc                                                                                                                                                              <  1:29070/248‑1 (MQ=255)                                                                           gTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATg                                                                                                                                                          <  2:370520/250‑1 (MQ=255)                                                                           gTTAACGGCGCTGGATTTCAAA‑CCGCTTCAGTTGCATGACCAGAACGAACGCCCGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGAt                                                                                                                                                           <  2:335444/249‑1 (MQ=255)                                                                                  gagcTGGATTGCAAAACCG‑ATCAGTTGCCTGACCATAACTAACCAACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAAATGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCATGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTGGCCCGATAGACGCTGATGGTTTCTGCCCGAAGGAGAAGtt                                                                                                                                                  <  2:242837/249‑1 (MQ=255)                                                                                    gcTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTg                                                                                                                                                <  2:146792/251‑1 (MQ=255)                                                                                    gcTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTg                                                                                                                                                <  1:372505/251‑1 (MQ=255)                                                                                     cTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTg                                                                                                                                                >  1:187291/1‑250 (MQ=255)                                                                                        gATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGCTGGATAAGTTTGAt                                                                                                                                              >  2:248413/1‑249 (MQ=255)                                                                                         aTTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGc                                                                                                                                           <  2:250942/251‑1 (MQ=255)                                                                                            tCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCgt                                                                                                                                         <  1:116447/250‑1 (MQ=255)                                                                                                  ccGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTAGCGGCAACATCGTTTTCCATCGTTGGCATGACAGAAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTCCGTAATGTCGATGCGTTAACACTGCAGGCACCTGCGCAGCGTGGGACGCTGGAATTAAGCGGTTGCCCGGTAGACGCTGATGGTTTCTGCCCCATGGATAAGTTTGATAGCGTGTtga                                                                                                                                    >  1:49690/1‑250 (MQ=255)                                                                                                    gTATCAGTGGCATGACCAGAACGAACGCACGCCGAGTGGCGTCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGAGGAAAATTGAATAGGTGGATCAGAGTGCGGAACAGTTACGGAATGTCGATGCGTTAACCCGGCATGCAGCTGCGCAGCTTGTGACGCTGGAATTAAGCGGGTGCCCTATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTtgaatg                                                                                                                                 <  1:477723/251‑1 (MQ=255)                                                                                                         aGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCg                                                                                                                            <  2:205586/251‑1 (MQ=255)                                                                                                                tGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAAt                                                                                                                     >  1:40726/1‑251 (MQ=255)                                                                                                                         cgaacgCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGTTGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGaaaa                                                                                                            <  2:102380/251‑1 (MQ=255)                                                                                                                         cgaacgCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCTGTGAAATAACAGaaa                                                                                                             >  1:257915/1‑250 (MQ=255)                                                                                                                          gaacgCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAAc                                                                                                           >  1:295883/1‑251 (MQ=255)                                                                                                                                acgcCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTccccc                                                                                                     <  1:306668/251‑1 (MQ=255)                                                                                                                                           cggcAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGGCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCgg                                                                                          <  2:381533/251‑1 (MQ=255)                                                                                                                                               aaaaTCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGa                                                                                      <  2:37792/251‑1 (MQ=255)                                                                                                                                                 aaTCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGAGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCGGCGGGAA‑Cggggggggc                                                                                   >  1:2376/1‑247 (MQ=255)                                                                                                                                                  aTCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATGTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAATCGGGGGAGTc                                                                                   <  2:433922/251‑1 (MQ=255)                                                                                                                                                     gTTTTCCAGCGTTGGCATGACAGCAAAGCCAAGCGCGATTTGATGAAAATTGAATATGTGGATCAGAGTGCGGAACAGGTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTGCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCTCCCGCGAGAAGCGGGGGAGTCGCt                                                                                <  1:44635/251‑1 (MQ=255)                                                                                                                                                      ttttCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGCTGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                                                               >  1:267872/1‑251 (MQ=255)                                                                                                                                                          ccAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTa                                                                           <  1:37391/251‑1 (MQ=255)                                                                                                                                                                                  caactgcgATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGGTACGTAATGTCGATGCGTTAACCCTGCAGGCGCCTGCGCAGCGTGTGACGCTGGAATTAAGCGGGTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTCTTACGTTCGATGGTCTg                                                   <  2:332317/246‑1 (MQ=255)                                                                                                                                                                                         gATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGccc                                            >  2:305987/1‑251 (MQ=255)                                                                                                                                                                                             tgatgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCaa                                         >  1:295545/1‑250 (MQ=255)                                                                                                                                                                                               atgaAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTATCCCTGCAGGCACCTGCTCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATATCAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCAGTTCGCCCCaaaaa                                      <  1:376073/251‑1 (MQ=255)                                                                                                                                                                                                       tGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt                               <  1:35793/250‑1 (MQ=255)                                                                                                                                                                                                         aaTATGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCtt                            <  1:125678/251‑1 (MQ=255)                                                                                                                                                                                                          atatGTGTATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCAACTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCttt                           >  1:311992/1‑251 (MQ=255)                                                                                                                                                                                                                gtATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCg                       <  1:381775/249‑1 (MQ=255)                                                                                                                                                                                                                gtATCAGAGTGCGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCg                       >  2:381775/1‑249 (MQ=255)                                                                                                                                                                                                                           cGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                                                               <  2:114167/182‑1 (MQ=255)                                                                                                                                                                                                                           cGGAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                                                               >  1:114167/1‑182 (MQ=255)                                                                                                                                                                                                                            ggAACAGTTACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCACAGAGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAACGGc         >  1:170364/1‑251 (MQ=255)                                                                                                                                                                                                                               aCAGTTTCGTAATGTCGATGCGTTAACCCGTCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTTCGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCttt      <  1:311848/251‑1 (MQ=255)                                                                                                                                                                                                                               aCAGTTACGTAATGTCGAGGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTTGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCttt      <  1:324778/251‑1 (MQ=255)                                                                                                                                                                                                                                    tACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTTCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGGTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAATGCTTTgtct  <  1:439277/250‑4 (MQ=255)                                                                                                                                                                                                                                    tACGTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACt  <  1:439270/250‑1 (MQ=255)                                                                                                                                                                                                                                       gTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcc                                                                                                        <  1:30620/145‑1 (MQ=255)                                                                                                                                                                                                                                       gTAATGTCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTcc                                                                                                        >  2:30620/1‑145 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                 GCTCTGGTCACCGATCGCACCAGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACGGCGCTGGATTTCAAA‑CCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGCAAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAATATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACT  >  NC_000913/1066464‑1066939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑