Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	96.4%	164.2 / ‑2.2	57	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (29/25);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGA  >  NC_000913/822283‑822772                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        ggCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑g                                                                                                                                                                                                                                                  <  1:160771/251‑1 (MQ=255)   cGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GAt                                                                                                                                                                                                                                                >  1:261613/1‑251 (MQ=255)    gAGCAGGTCATAGCAGCCATAAATCGGATAGCCGACGAACACCAGCCCGACCGCACTCAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAAACATCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACCCAATAACCGCGATAAAAACCCGCCCGGTGAGGATCTTCTTTGCTAAGCGCCTGGGCGGGTCTGACTTACACATCAGGGATTTATCCCCGTGTATGCAGTTTTTACCC‑GATc                                                                                                                                                                                                                                               >  2:340158/1‑251 (MQ=255)            cATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCTCTAAGCAGCGCAACGCGATTGTATTCGCGGATGACCTTCCAGACCTCTTCCCATTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTGTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGttt                                                                                                                                                                                                                                       <  1:469249/251‑1 (MQ=255)            cATAGCAGCCGTAAATCAGAGAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACTCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGTCCACCAGCAACACAATCACCTCGATAAAAAACAGCCAGGTGAGGATCGTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGttg                                                                                                                                                                                                                                       <  1:469246/251‑2 (MQ=255)                     cGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCg                                                                                                                                                                                                                              >  1:214948/1‑251 (MQ=255)                      gTAAATCAGATAGCTGACGACCACCAGCCCGACCGCAATAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCC‑GATCCTGCGTTTCCATTGTCgg                                                                                                                                                                                                                             >  1:97462/1‑251 (MQ=255)                                     gatgacCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATc                                                                                                                                                                                                              <  1:169731/248‑1 (MQ=255)                                          acacccGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCAAGGTGAGGATATTCATTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGCTT‑TACCCCGATCCTGCGTTTCCATTGTCGGGTATgcaggcgggtacacct                                                                                                                                                                                                         >  2:353507/2‑243 (MQ=255)                                                              agcagcGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCgtgtgg                                                                                                                                                                                     >  1:143452/1‑250 (MQ=255)                                                              agcagcGCAAAGCGATTGAAGTCGCGGACGACCTTCCCGAACTCTACCCCGTCCACTTTTATGGCGTAGACCACCAGCCACACAATCACCGCGCTAAAAAACAGCACGGTGAGGATCTTCTTTGCTAAGCGCCAGAGCAGGTGTGATTTACTCATCAGGGTTTTCAACCCGTGTTTTCAGTTTTTGCCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGGTCacccctagccacacgtggcgggggt                                                                                                                                                                                     >  2:487226/1‑227 (MQ=255)                                                                cagcGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGGGGCGTGTTTGc                                                                                                                                                                                   >  2:427973/1‑251 (MQ=255)                                                                 agcGCAACGCGATTTTAGTCGCGGATGTCCTTCCAGACCCCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGcc                                                                                                                                                                                  <  2:348448/251‑1 (MQ=255)                                                                 agcGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGcc                                                                                                                                                                                  >  1:55450/1‑251 (MQ=255)                                                                        cgcgATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCAAGTCCACTTTTTTGGCGTAGCCCACCAGCACCACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGAGATTTACTCATCAGGGTTTTACCCCCGAGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCCTTGTCGGTTGTGCGGGCGGATCAACCTGCGCCCAACGTGGCGTGTGGGCCgggaacc                                                                                                                                                                           >  2:464803/1‑246 (MQ=255)                                                                              tGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTGTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAgccagcca                                                                                                                                                                      <  1:336837/250‑1 (MQ=255)                                                                                  gTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGCAGCCAGCCAAc‑c                                                                                                                                                                  >  1:439517/1‑249 (MQ=255)                                                                                         aTGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCggg                                                                                                                                                         <  1:63514/251‑1 (MQ=255)                                                                                           gACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATACAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTGGGGCGGGCGGATCAACCTGGGCCAGCAGTGGCGGGTGGGCCGGGAgccatccacaccgcgcccgga                                                                                                                                                         >  2:270896/1‑237 (MQ=255)                                                                                                   aGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGt                                                                                                                                                >  1:436615/1‑250 (MQ=255)                                                                                                        tctTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTaaaa                                                                                                                                            >  1:321521/1‑249 (MQ=255)                                                                                                                       tttttGGCGTAGACCACCAGCAACACAAGCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGTCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCAc                                                                                                                            <  2:358263/250‑1 (MQ=255)                                                                                                                        ttttGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACg                                                                                                                           <  1:139263/250‑1 (MQ=255)                                                                                                                          ttGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTCTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCATCACGCt                                                                                                                         <  1:435265/250‑1 (MQ=255)                                                                                                                               gTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCttt                                                                                                                       <  2:88403/247‑1 (MQ=255)                                                                                                                               gTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCACGCttt                                                                                                                       >  1:88403/1‑247 (MQ=255)                                                                                                                                 aGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGGGCGGGCGGGTCCACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCCCCGGGAAGGGGCGGAAAAAGGGGAACGCCAGCACGCTTTTgggt                                                                                                                  >  2:169846/1‑248 (MQ=255)                                                                                                                                   accaccAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAgg                                                                                                               <  1:401515/251‑1 (MQ=255)                                                                                                                                                    cACCGCGATAAAAACCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGtt                                                                                               <  1:211974/250‑1 (MQ=255)                                                                                                                                                          gATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTTCGCCAGACGTGGCGTGTGTGCCGTAAGCCATCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGgcag                                                                                        <  1:291830/251‑1 (MQ=255)                                                                                                                                                            tAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGgcagcc                                                                                      >  1:142806/1‑250 (MQ=255)                                                                                                                                                                 aaCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGtt                                                                                 <  2:142806/251‑1 (MQ=255)                                                                                                                                                                    aGCCAGGGGAGGATCTTCTTTACTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATACTGCGTTTCCATTGCCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGGTCCGGAAACCAGCCAACACA‑CGCCGGGAAGTGGCGTAAAAAGAGGAAAGCCCGCGCGCCTTTCGTCATGTTTCACCCGGTGGTTTTGGgcctgttggattc                                                                               >  2:440178/1‑239 (MQ=255)                                                                                                                                                                        aGGTGAGGATCTTCTTTGCTAAGCGTCTGCTCGGGTGTGATTTACTCATCAGGGTTGTACCCCCGTGTTTTCAGTTTTTACCC‑GAGCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTTCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGACCGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCa                                                                          <  2:340210/251‑1 (MQ=255)                                                                                                                                                                        aGGTGAGGATCTTCTTTGCTAAGCGCCAGCTCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCa                                                                          <  1:282887/251‑1 (MQ=255)                                                                                                                                                                        aGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATcc                                                                           >  1:83815/1‑250 (MQ=255)                                                                                                                                                                          gTGAGGATCTTCTTTGCTAAGAGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGGCAAAAGTGGAACGCCAGCCCGCTTTTGGTCAGGATCCACCCGGTGCGTTTGGGGAGCCAGGTTTTATCCAcc                                                                        >  2:122706/1‑251 (MQ=255)                                                                                                                                                                          gTGAGGATCTTATTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGATGTGCGGGCGGATCAAACTGCGCCAGACGTGGGGTGTGTGCCGGAAGCCCGCCAAC‑CAGCGCCGGGGAGTGGCGTCAAAAGTGGAACGCCCGCCCGCTTTTGGGCAGGGTCCACCCGGTGCGTTTGGGACGCATGGTTTCCTCCAcc                                                                        >  2:504007/1‑251 (MQ=255)                                                                                                                                                                             aGGATCTTCTTTGATAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCCGTTTCTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCCGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGGGGAAAAAGTGGAACGGTAGCACGCTTTTGGTCAGGTTCCACCAGGGGCGTTTGGGCAGCATGGTTTCATCCACctgc                                                                     >  1:110402/1‑251 (MQ=255)                                                                                                                                                                                    tcttTGCTAAGCGCCAGCGCGGGTGTGACTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTGTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGGCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAATTGGAACGCCAGCACGCTTTTGGGCATGTTCCGCCAGGGGCGTTTGGGCAGCATGGTTTCATCCACctgctg                                                                   <  1:168267/246‑1 (MQ=255)                                                                                                                                                                                        tGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGc                                                          >  1:133304/1‑251 (MQ=255)                                                                                                                                                                                           tAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGAGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGCGGCGTAAAAAGGGGAACGCCAGCAAGCTTTTGGTCAGGATCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGc                                                       >  1:81731/1‑251 (MQ=255)                                                                                                                                                                                                gcCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGGGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCaata                                                   >  1:187981/1‑250 (MQ=255)                                                                                                                                                                                                     cgcgGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTGTTCAGTTTTTACCC‑GGTCCGGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAATCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCGGCTGACAATCTGCGGCAATAATGCCg                                             <  2:426060/251‑1 (MQ=255)                                                                                                                                                                                                         ggtttgATTTCCTCAACAGGGTTTTACCCCCGTTTTTTCAGGTTTTTCCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGTGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCa                                         <  2:214640/247‑1 (MQ=255)                                                                                                                                                                                                          gttttaTTTATTCATCAGGGTTTTACCACCGTGTTGTCATTTTTTCCCC‑GATCCTGCGTTTCGATTGTTGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGTACGCTTTTGGTCAGGTTCCACCATGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGTCGTTCAg                                        <  2:431715/246‑1 (MQ=255)                                                                                                                                                                                                             tgATTTACTCATCAGGGTTTTACCCCCGTGTTTTCCGTTTTTACCC‑GATCCTGCGTTTCCAGTGTCGGTTGTGCAGGCGGATCAACCTGAGCCAGACGTGGCGTGTGTGCCGGAAGACAGCCAAC‑CAGGGCCGGGAAATGGCGTAAAACGGGGAACCCCAGCCCGCTTTTGGTCAGGCTTCACCAGGTGCGGTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGAGGCAATAATGGCGTTCAAAtt                                     >  1:6892/1‑251 (MQ=255)                                                                                                                                                                                                              gATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGAttt                                    >  1:426865/1‑250 (MQ=255)                                                                                                                                                                                                                 ttACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGGGCCGGAAGCCAGCCACC‑CAGCGCCGGGGAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATcg                                 >  1:69078/1‑251 (MQ=255)                                                                                                                                                                                                                   aCTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGACAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAATGGAACACCAGCACGCTTTTGGGCAGGGTCCACCAGGTGCGTTTGGGCAGCGTGGGTGCATCCACCTGCTGCCAATCTGCGGCAAGAATGCCGGTCAGATTATcgc                                >  1:323671/1‑250 (MQ=255)                                                                                                                                                                                                                             gTTTTCCCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTgg                     <  1:469923/251‑1 (MQ=255)                                                                                                                                                                                                                              ttttACCCCCGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGGGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGt                    >  1:378419/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCCCCCGTGTTTTCAGTTTTTACCC‑GCTCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCTTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTaa                 <  2:402320/250‑1 (MQ=255)                                                                                                                                                                                                                                      ccGTGTTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCCGCTGGCAATCTGCGGCAATAATGCCGTTCAAATTATCGCGCAGGGTCAGGTTTAAATg             >  2:198704/1‑250 (MQ=255)                                                                                                                                                                                                                                          gtTTTCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGAt        <  1:427973/251‑1 (MQ=255)                                                                                                                                                                                                                                            tttCAGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCTGACGTGGCGTGTGTGCCGGAAGCCAGACAAC‑CAAGGCCGGGAAGTGGCGTAAAAAGAGGAACGCCAGCACGCCTTTGGTCAGGTTCCACCCGGTGCGTTTGGGCAGCATGGGTTCATCAACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCTGAGTCTGGTTAAAATggagatgg      >  2:465925/1‑244 (MQ=255)                                                                                                                                                                                                                                                aGTTTTTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGga  <  1:250340/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        GGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCC‑GATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAC‑CAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGA  >  NC_000913/822283‑822772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑