Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913721,6040GA95.3% 120.9 / ‑1.8 44R44C (CGT→TGT) kdpEDNA‑binding transcriptional activator KdpE
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (18/23);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

GGGCGCGGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATC  >  NC_000913/721359‑721846
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
gggCGCGGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCCATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAAACCCATAACCCCCGGGCCGGCCCAGATCGGGAATAATCAAATCAGGCTTACCGGttt                                                                                                                                                                                                                                                  >  2:416454/1‑250 (MQ=255)
     gggggggggccggggtgcggcgTAAGGCGACGCGCAGACGTGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTGCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGcg                                                                                                                                                                                                                                             <  2:52478/235‑2 (MQ=255)
         ggtgtCAGAGTGGCGGCGTAATGCGACGCGCATACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCa                                                                                                                                                                                                                                        <  1:484589/251‑1 (MQ=255)
              gagagTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAg                                                                                                                                                                                                                                   <  1:433723/250‑1 (MQ=255)
                    ggcggcGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAAGGCCAAACGGCTTACTCAGATAATCATCCGCTCCGCCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTACGTGCGGAAAGCAAAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGAGCAGGCCGAGATCGAGCATAATCAAATCTGGCTTCCAGGTTGCAGC‑TTCCAGCAATCCGCGt                                                                                                                                                                                                                             >  2:57475/1‑251 (MQ=255)
                           tAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCg                                                                                                                                                                                                                      >  1:338206/1‑251 (MQ=255)
                                  aCGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGc                                                                                                                                                                                                               >  1:475825/1‑251 (MQ=255)
                                        aGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCaa                                                                                                                                                                                                          >  2:211641/1‑250 (MQ=255)
                                         gtcggtctggCAATTCGCCATTGCCAAACGGCTTACTCAGATAATCATCCGCACCGGCATCCATCGCGTCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGAGCGAGAATAATCAAATCTGGCTTACAGGTTGCCTC‑TTCCATCAAGCCGCGTTGCAGCGTGTCGCCCTCCAAg                                                                                                                                                                                                        <  2:81977/242‑1 (MQ=255)
                                                  gtaaTTCGCCAAGGGCAAACGGCTTACTCATATAATCATCCGCGCCGGCATCCAGCGCGGCGAGTTTGTCGCGCTCTTCGCTGCGTGCGGAAAGCACAAGCACCGGCCCCGCGCTCCAAGGGCGCAGGTCGCGGATAAACTCAATCCCAGCACCATCGGGCAGGCCGAGATCGAGAAGAATCAAAACTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGCCGCGCATc                                                                                                                                                                                               <  2:246699/249‑1 (MQ=255)
                                                     aTTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCg                                                                                                                                                                                            <  1:252515/251‑1 (MQ=255)
                                                                          tACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCGGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTgcg                                                                                                                                                                        <  2:372704/250‑1 (MQ=255)
                                                                                     ttatcCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGGCGCCCCCCAGCGCCGtcagccgcaagcgacc                                                                                                                                                            >  2:60734/3‑236 (MQ=255)
                                                                                     tcatcCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGcgacg                                                                                                                                                            >  1:499752/1‑251 (MQ=255)
                                                                                        tcCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAAt                                                                                                                                                         >  1:74415/1‑251 (MQ=255)
                                                                                             tCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAGGCGACGAATAGCCt                                                                                                                                                    >  1:13664/1‑251 (MQ=255)
                                                                                                     ccagtgtggcgATTTTGTCGCTCTCTTCGCTGCGGGCGGAAAGCACAATCCCCGGCACCGCGCTCCACTGGCGTAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTCCGGCCTCAAAGGCGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATc                                                                                                                                             <  2:145427/243‑1 (MQ=255)
                                                                                                       aGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTAATCTTc                                                                                                                                          >  1:398590/1‑251 (MQ=255)
                                                                                                            ggcgATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTcaacaa                                                                                                                                     <  1:416454/251‑1 (MQ=255)
                                                                                                            ggcgATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTcaacaa                                                                                                                                     <  1:1927/251‑1 (MQ=255)
                                                                                                                              ttCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACat                                                                                                                 >  1:159199/1‑251 (MQ=255)
                                                                                                                                    gcgtgcgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCTACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCt                                                                                                            <  1:25987/250‑1 (MQ=255)
                                                                                                                                      gtgcgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCAt                                                                                                         <  1:411898/251‑1 (MQ=255)
                                                                                                                                      gtgcgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCAt                                                                                                         <  2:475825/251‑1 (MQ=255)
                                                                                                                                      gggcgGAAAGCACAATCACCGGCACCGCGCTCCACGGGCGCAGGTCGCGGATAAACTCAATCCCATCACCAGCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATAGCCTCAt                                                                                                         <  2:74415/249‑1 (MQ=255)
                                                                                                                                         cgGAAAGCACAATCCCCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAACCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGaa                                                                                                      <  2:446069/251‑1 (MQ=255)
                                                                                                                                         cgGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCACTCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCCTCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATCGCCTGTTCTTCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGa                                                                                                       >  2:222440/1‑250 (MQ=255)
                                                                                                                                                             accgctccccACTGGCGCAGGTCGCGGCTAAACTCAACCCCAGCACCATCGGGCAGGCCGAGAGCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCa                                                                                  <  2:338206/243‑1 (MQ=255)
                                                                                                                                                                      cACTGGCGCAGGTAGCGGATAAACTCAACCCCATCACCACCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGCTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCTGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTgg                                                                         <  2:267210/251‑1 (MQ=255)
                                                                                                                                                                              cAGGTCGCGGATAAACTCAATCCCCTCTCCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGATGACAGGTTGCCGT‑GTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCTTCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTTATGAAATTCTTCAAGTTCAGGGGCAGTGTGCTGGGGAAgtgt                                                                 <  2:403204/251‑1 (MQ=255)
                                                                                                                                                                               aGGTCGCGGATACACTCAATCCCATCACCATCGGGCAGGCAGAGCTCGAGGATAATCAAATCTGGCTTACAGGTTCCCGC‑TTCAAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAGAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAACGCGACGACTAGCCTGGTCATCTTCC‑CAACTCAGCACG‑CTTGTCCAATATCCTCATGAAATTATTCAAGTTCAGGGGCAGTTTGCTGGGGAAgtgt                                                                 >  2:56374/1‑250 (MQ=255)
                                                                                                                                                                                   cgcgGCTAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGCGACTAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCACGTTGCAGCGTTTCGGCCTCAAAGACGCGCCTCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGCATAGCCTGTTCATCTTCAACAA‑TCAGACCG‑TTTGTCACATATCCTCATGACATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAacac                                                            >  2:28945/1‑251 (MQ=255)
                                                                                                                                                                                     cgGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACg                                                           >  1:304905/1‑250 (MQ=255)
                                                                                                                                                                                                  ccccATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGcaccac                                             <  1:374427/250‑1 (MQ=255)
                                                                                                                                                                                                   cccATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCaccacc                                            <  1:328246/251‑1 (MQ=255)
                                                                                                                                                                                                   cccATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGCAACACGAAAACAGGCaccacc                                            <  1:465882/251‑1 (MQ=255)
                                                                                                                                                                                                    ccATCCCCATCGCCCAGGCCGAGATCGAGAACAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGCAAGCGACGACTAGACTGTTCATCTTCAACAA‑T‑AAAACGGTTTGTCACGTAATCTCAGGAAATTCTTCAAGTTCAGTGGCAGTTTGCTGGGGAAGTGTAACACGaaataaggacccatct                                           >  2:489949/1‑238 (MQ=255)
                                                                                                                                                                                                              cGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCcggtcggt                                 >  1:3946/1‑251 (MQ=255)
                                                                                                                                                                                                                              gagaATAATCAAATCTAGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGATTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGACATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACGTTCCGGTCGGTTGAAggcgtgaat                    >  2:349040/1‑243 (MQ=255)
                                                                                                                                                                                                                               agattaatCAAATCTGGCGTACAGGTTGC‑GCGTGCCAGCAAGCCGCTTGGCAGCGTTTCGGCCGCAAAGACGCGCATCCCGTCTCCCTCCAGCGCCGTTCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCACATGAAATTCTTCAAGTGCAGGGGCAGTTTGCTGGGGAAGTGTAACACTAAAACAGGCACCACATTCCGGTCATTTTAACGCGGTAATAGTg                <  2:312226/247‑1 (MQ=255)
                                                                                                                                                                                                                                     atCAAATCTGGCTTACGGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGcccc                                               >  2:40905/1‑211 (MQ=255)
                                                                                                                                                                                                                                     atCAAATCTGGCTTACGGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGcacc                                               <  1:40905/214‑1 (MQ=255)
                                                                                                                                                                                                                                      tCAAATCTGGCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCgt         >  1:39809/1‑251 (MQ=255)
                                                                                                                                                                                                                                              ggCTTACAGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATc  <  1:387855/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
GGGCGCGGTGGTGGCAGAGTGGCGGCGTAATGCGACGCGCAGACGGGCCTGCAATTCGCCAATGCCAAACGGCTTACTCAGATAATCATCCGCTCCGGCATCCAGCGCGGCGATTTTGTCGCTCTCTTCGCTGCGTGCGGAAAGCACAATCACCGGCACCGCGCTCCACTGGCGCAGGTCGCGGATAAACTCAATCCCATCACCATCGGGCAGGCCGAGATCGAGAATAATCAAATCTGGCTTACGGGTTGCCGC‑TTCCAGCAAGCCGCGTTGCAGCGTTTCGGCCTCAAAGACGCGCATCCCGTCGCCCTCCAGCGCCGTGCGCAGAAAGCGACGAATAGCCTGTTCATCTTCAACAA‑TCAGAACG‑TTTGTCACATATCCTCATGAAATTCTTCAAGTTCAGGGGCAGTTTGCTGGGGAAGTGTAACACGAAAACAGGCACCACCTTCCGGTCGGTTGAACGCGGTAATAGTGCCCCCGTGTACATC  >  NC_000913/721359‑721846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑