Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	674,259	G→A	C175C (TGC→TGT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	674,259	0	G	A	93.8%	84.6 / ‑1.8	33	C175C (TGC→TGT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (16/14);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

GTACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGG  >  NC_000913/674014‑674489                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       gtaCAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGcc                                                                                                                                                                                                                                   >  1:82201/1‑251 (MQ=255)         cATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATa                                                                                                                                                                                                                            >  1:26027/1‑250 (MQ=255)          aTAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCCCGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAAc                                                                                                                                                                                                                          <  2:448158/251‑1 (MQ=255)          aTAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGCTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCACCTTTGGTATCGCAGCGCCAGCACCAGCCGGCGATacc                                                                                                                                                                                                                          >  2:25963/1‑249 (MQ=255)                                          cAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGTCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCg                                                                                                                                                                                          >  1:132839/1‑251 (MQ=255)                                          cAGCGTGTTGGCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGTTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCg                                                                                                                                                                                          >  2:76385/1‑251 (MQ=255)                                                  tgtCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCa                                                                                                                                                                                   <  1:260447/250‑1 (MQ=255)                                                  ggtCATAGTCGTTAACGTTGAAGGGGATCTCCACGCCTTCGGAACGCCCGATCCAGTTACGCTGCATGGTTTTAACGGTGGCTGGCCAGTGATCCAGGTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAg                                                                                                                                                                                  <  2:390370/250‑1 (MQ=255)                                                     cATAGTCGTTATCGTTTAAGTTGATCTCCCCGCCTTCGGAACGACCGTTCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTTATCCAGTTTATCCAGATCGTTGAGCATCTCGTCAGCGTAAGCAGTGATTTTGATAAACCCCTGCGGGATCTCTTTTCGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATATCTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGtt                                                                                                                                                                                <  2:369329/250‑1 (MQ=255)                                                                         gTGATTTCCACGCCTTCGGAACGACCGATCCAGGTACGCGGCATGGTTGTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTtata                                                                                                                                                           <  2:111942/251‑1 (MQ=255)                                                                                ccACGCCTTCGGAACGACCGATCCAGTTACGGTGCAGGGTTTTAACGGTGTCGGGACAGTGATCCAGTTTAGCAAGATCGTTGAGCAGCTCGGAAGCGGAAGCAGTGATTGTGATAAACAACTGCGGGATCTCTTTACGTTCAACTGTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGGCTGGTCGGTCGGGCACCAGTTGACCGCAGAAGTCTTCGTATATAcaa                                                                                                                                                      <  2:240614/249‑3 (MQ=255)                                                                                           gAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTtat                                                                                                                                          >  2:281286/1‑250 (MQ=255)                                                                                             aCGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACa                                                                                                                                       <  1:237694/251‑1 (MQ=255)                                                                                                cccGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTTGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCATCGTAATCAGTGATTTTGATAAACCATTGCGGGATCTCTTTACGTTCAACTTTTGTATCGCAGCGCCAGCAATAGCCGTCGATAACTTGTTCTTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCt                                                                                                                                    <  1:322008/250‑1 (MQ=255)                                                                                                            ccGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTGTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAAtt                                                                                                                        <  1:10947/250‑1 (MQ=255)                                                                                                               cTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGCAGTCTTCTTATATACCCGGCCTTTTTTATACAGCTCGGTGAAGAATTTCt                                                                                                                     >  2:250984/1‑251 (MQ=255)                                                                                                                           aaCGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGGTGAGCAGCTCGTCAGCGTAATCAGTGATTTTGATAAACCACTGCGGGATCTCTTTCCGTTCAACTTTTGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGTCCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACggc                                                                                                         <  2:16248/251‑2 (MQ=255)                                                                                                                               gtgtCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCACCGgtagt                                                                                                      >  2:284479/1‑250 (MQ=255)                                                                                                                                                      aTCCAGATCGTTGTGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCCATGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGGTGACCGCAGAAGGCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGGATTACGGCGTACAGCTTGCCAGCt                                                                              >  2:193548/1‑251 (MQ=255)                                                                                                                                                         cAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTcgc                                                                           >  1:415999/1‑251 (MQ=255)                                                                                                                                                          aGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTcgcg                                                                          >  2:420236/1‑251 (MQ=255)                                                                                                                                                              cGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCTCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCt                                                                       <  1:484012/250‑1 (MQ=255)                                                                                                                                                                    gcagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAAt                                                                                                                         >  2:304276/1‑194 (MQ=255)                                                                                                                                                                    gcagcTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAAt                                                                                                                         <  1:304276/194‑1 (MQ=255)                                                                                                                                                                        cTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAAc                                                            >  1:49262/1‑251 (MQ=255)                                                                                                                                                                                  tAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGccc                                                    >  1:155631/1‑249 (MQ=255)                                                                                                                                                                                                gATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTgg                                     <  1:62124/250‑1 (MQ=255)                                                                                                                                                                                                      ccACTTCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCTTTCGCCAGTACTGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGTTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGTCGTACAGGGTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCa                              <  1:504398/251‑1 (MQ=255)                                                                                                                                                                                                                aTCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTAAGGTCTGGTCGCTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTTAGCTGGCTTTTCATATACGCGAt                    >  1:323325/1‑251 (MQ=255)                                                                                                                                                                                                                 tctcTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAACACCCAGCATTTTGAGCTGGTTTTTCATATACGCGAtg                   >  1:322396/1‑251 (MQ=255)                                                                                                                                                                                                                          gTTCAACTTTGGTCTCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTa           <  2:156908/250‑1 (MQ=255)                                                                                                                                                                                                                          gTTCAACTTTGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTa           >  1:156908/1‑250 (MQ=255)                                                                                                                                                                                                                                  ttGGTATCGCAGCGCCAGCAACAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAACCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACgg  >  1:347766/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       GTACAACCCATAAAGGTGTCCGGGCGGGTAGTGTAAACGGTCAGCGTGTTGTCATAGTCGTTAACGTTGAAGGTGATCTCCACGCCTTCGGAACGACCGATCCAGTTACGCTGCATGGTTTTAACGGTGTCTGGCCAGTGATCCAGTTTATCCAGATCGTTGAGCAGCTCGTCAGCGTAAGCAGTGATTTTGATAAACCACTGCGGGATCTCTTTACGTTCAACTTTGGTATCGCAGCGCCAGCAGCAGCCGTCGATAACTTGTTCGTTCGCCAGTACGGTCTGGTCGTTCGGGCACCAGTTGACCGCAGAAGTCTTCTTATATACCAGGCCTTTTTTATACAGCTCGGTGAAGAATTTCTGTTCCCAACGGTAGTATTCCGGCGTACAGGTTGCCAGCTCGCGGCTCCAGTCATAACCAAAGCCCAGCATTTTGAGCTGGTTTTTCATATACGCGATGTTGTCGTACGTCCACGG  >  NC_000913/674014‑674489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑