Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 602,835 G→A A293T (GCT→ACT)  pheP → phenylalanine:H(+) symporter PheP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913602,8350GA98.2% 164.2 / ‑4.9 54A293T (GCT→ACT) phePphenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/19);  minor base C (1/0);  total (35/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

CTGATTTTGTCGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGG  >  NC_000913/602589‑603081
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
cTGATTTTGTCGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGGTTTTCCATAATCTCGACAGCAACGTGGTAACTTc                                                                                                                                                                                                                                                    >  1:263871/1‑251 (MQ=255)
                   tacttATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGGGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATCATCTCGACAGCAACGAGTTAACTTCTGCGCTGAACTTTGTCAtt                                                                                                                                                                                                                                 >  1:450719/4‑251 (MQ=255)
                   tAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCAtt                                                                                                                                                                                                                                 >  1:355097/1‑251 (MQ=255)
                               ccTTCGGCGGTCTTGAGCTTATTGGGATTACTGCCGCTGAAGCGCGCGAGCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGATTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTACCATAATCTCGACAGCAACGTGGTAACTTCGGCGCTGAACTTCGTCATTCTGGTAGCATCg                                                                                                                                                                                                                     <  1:406455/251‑1 (MQ=255)
                                  tCGGCGGTCTGGAGCTGTTTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGAGGGTGGTAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGCCAACAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTCGCATCGcgg                                                                                                                                                                                                                  >  2:217098/1‑249 (MQ=255)
                                    ggcggTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTc                                                                                                                                                                                                                >  1:413592/1‑251 (MQ=255)
                                            ggAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGta                                                                                                                                                                                                          <  2:497756/249‑1 (MQ=255)
                                                       ggATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAAAGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGtt                                                                                                                                                                                             >  1:59090/1‑251 (MQ=255)
                                                               gCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTa                                                                                                                                                                                      >  1:133659/1‑250 (MQ=255)
                                                               gCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGACGTATCGCATCCTGCTGTTTTACATCGGTTCACAGGTGTTTTTACTGGCGTGCTAGCCGGGGGTGGAATCGAAAGCCAACAGTAGCCCGTTTGTGATGATTTTCCATAAGCTCGACAGCAACGTGTTAACTGCTGCGCTGAACTTCTTCATTCTGGTAGCATCGCTGTCAGTGTATAACATGTGGGTTTActc                                                                                                                                                                                        <  2:450719/248‑1 (MQ=255)
                                                                          gcgcgATCCGGAAAAAAGCATTCCAAAGGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGc                                                                                                                                                                          <  2:336481/251‑1 (MQ=255)
                                                                                  cGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGc                                                                                                                                                                  <  2:413592/251‑1 (MQ=255)
                                                                                         aaGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCttt                                                                                                                                                              >  1:502820/1‑248 (MQ=255)
                                                                                         aaGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCGGTTTTACATCGGTTCACTGGTGTTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCttt                                                                                                                                                              <  2:502820/248‑1 (MQ=255)
                                                                                            cATTCCAAAAGCGGTAACTCCGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGCAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGCCAGCAACGTGGTAAATTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTCCTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGc                                                                                                                                                        >  2:251743/1‑251 (MQ=255)
                                                                                                       cGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATAGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATgcg                                                                                                                                             >  1:167527/1‑251 (MQ=255)
                                                                                                            aaTCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGa                                                                                                                                         >  1:157260/1‑250 (MQ=255)
                                                                                                            aaTCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAACATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAAc                                                                                                                                                                                    >  1:297914/1‑207 (MQ=255)
                                                                                                                     gtgtATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATTCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGa                                                                                                                                <  1:448830/250‑1 (MQ=255)
                                                                                                                        tATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTATAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTcg                                                                                                                            >  2:400891/1‑251 (MQ=255)
                                                                                                                             cATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTc                                                                                                                        >  2:207228/1‑250 (MQ=255)
                                                                                                                                ccTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGcc                                                                                                                    >  1:419205/1‑251 (MQ=255)
                                                                                                                                   gctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGACGTGAAATACAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTc                                                                                                                 >  1:159612/1‑251 (MQ=255)
                                                                                                                                   gctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGttttagatcggaagagcaccgt                                                                                                                 >  1:95967/1‑233 (MQ=255)
                                                                                                                                   gctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTc                                                                                                                 >  1:16109/1‑251 (MQ=255)
                                                                                                                                   gctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCGTTCTGTGCAGGGTAATGCGCCGAAGtttt                                                                                                                                   <  2:95967/233‑1 (MQ=255)
                                                                                                                                    ctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGt                                                                                                                                                                                              <  2:423137/173‑1 (MQ=255)
                                                                                                                                    ctgTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGt                                                                                                                                                                                              >  1:423137/1‑173 (MQ=255)
                                                                                                                                      gTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTcgcg                                                                                                              <  2:33856/251‑1 (MQ=255)
                                                                                                                                       ttttACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGACGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCACCGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTTCAGGGTATTGCTCAGAAGttttttgttcgcttcccccgtcgcgg                                                                                                             >  2:375492/1‑230 (MQ=255)
                                                                                                                                       ttttACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATACTCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGAGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGGAATGCGCCGACGTTTTTGACTCGCGTCAGCCGTCGCgg                                                                                                             >  2:272771/1‑251 (MQ=255)
                                                                                                                                                   tCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTa                                                                                                  >  1:363065/1‑250 (MQ=255)
                                                                                                                                                      cTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTc                                                                                              >  1:240019/1‑251 (MQ=255)
                                                                                                                                                        ggtggtTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCAATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTc                                                                                              <  2:380999/249‑1 (MQ=255)
                                                                                                                                                                   tGGCGCTCTATCCGTGGGTGGCAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGCCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTcc                                                                                 >  2:481708/1‑251 (MQ=255)
                                                                                                                                                                    ggctctttatctgggggtggAAGTGAAATCCAACAGGAGCCCGTTTGTAATGATTTTCCATAATCTCGACAGCAACGTGTTAACTTCTGCGCTGAACTTCGTCATGCTGGTAGCTTCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGTCTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTcc                                                                                 <  2:263272/236‑1 (MQ=255)
                                                                                                                                                                    ggcgctagaTCCGTGGGAGGAAGTGAAATCCAACAGCAGCCCGTTTGTGATGCTTTTCCATCATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTCGCCTCGCTGTCAGGGTATAACAGCGGGGTTTACTCTGACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCAGAAGTTTTTGCCTCGAGTCAGACGTCGCGGGGTGCCGATAAACTCGCTGATGCTTTCCg                                                                                >  2:310779/9‑251 (MQ=255)
                                                                                                                                                                     gcgcTCTATCCGTGGGTGGAAGTGAAATCAAACAGCAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTACCTTCTGCGCTGAACTTCGTCATTCTTGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGTCCTTTCTGTGCAGGGTATTGCGCCGAAGTTTTTGACTCGCGTCAGCCTTCACGGTGTGCCGATTAACTACCTGATGCTTGCCgg                                                                               >  2:245398/1‑251 (MQ=255)
                                                                                                                                                                      cgcTCTATCCGAGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTAAGTGTCTAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCATCGCGGAGAGCCGATTACCCCGCTGATTCTTTCCgg                                                                               >  2:152917/1‑250 (MQ=255)
                                                                                                                                                                        ctctATCCGAGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGGGCCGATTAACTCGCTGATGCTTTCCggtgc                                                                            >  2:78399/1‑248 (MQ=255)
                                                                                                                                                                            aTCCGGGGTTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGAt                                                                         <  2:411580/250‑1 (MQ=255)
                                                                                                                                                                              ccGTGGGTGGAAGTGAAATCCAACAGTAGCCCTTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGTGGTTTACTCTAACAGCCGCATGCTGTTTGTCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCAc                                                                      <  1:491628/251‑1 (MQ=255)
                                                                                                                                                                                gtgggtggAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGAAGCTTTCCGGAGCGAtacctt                                                                    >  2:260694/1‑246 (MQ=255)
                                                                                                                                                                                 tgggtggAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGAGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGCCTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTc                                                                   >  2:180090/1‑251 (MQ=255)
                                                                                                                                                                                    gtggAAGTGAAATCCAACAGTATCCCGTTTTTGTTTATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTATCAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCt                                                                <  2:289519/251‑1 (MQ=255)
                                                                                                                                                                                       gAAGTGAAATCCACCAGTAGCCCGTTTCTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGTGCTGAACTTCGTCATTCTGTGAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCGGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACACGCTGATGCTTTCCGGAGCGATCACGTCGCtggt                                                             <  2:336709/251‑1 (MQ=255)
                                                                                                                                                                                                      aGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCTTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTa                                              <  1:110982/251‑1 (MQ=255)
                                                                                                                                                                                                        gagaccGTTTGTGATGATTTTCCATAATCCTGACCGCATCGTGGGAACTTTTGGGCTGAACTTCGGCATTCGGGTAGCAGCGCTGTCGGGGTTTAACAGCGGGTTTTACTCTAACAGCCGCATGCTGTTTGGCCTGGCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCTTCAGCCGGCGCTGTGTGCCGATTAACTCGCTGATGCTTCCCGGGGCGATCACTTCTCTGGTGGTGTTAAGCAAccat                                             <  2:204432/246‑4 (MQ=255)
                                                                                                                                                                                                            ccGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGCAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATctgct                                        >  1:288136/1‑251 (MQ=255)
                                                                                                                                                                                                               tttGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCCCTTCGCTGGTGGTGTTAATCAACTATCTGCTGcc                                     >  2:431817/1‑251 (MQ=255)
                                                                                                                                                                                                                             ccATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTATCATCGCTGTCAGTGTATATCAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCGTTCTGTGCAGGGTAATGCGCCGAAGTTTGTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACGCGCTGATGCTTTCCGGAGCGATCTCTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGttt                        <  1:53311/250‑1 (MQ=255)
                                                                                                                                                                                                                              cATAATCTCGACAGCAACGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGGGCGATCACTTCGCTGGTGGGGTTAATCAACTATCTGCTGCCGCCAAAAGCGTTTgg                      >  2:225975/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cGTGGTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCtgg     >  1:109495/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ggTAACTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCtggtgg  >  1:348380/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
CTGATTTTGTCGCTGGCGGTAATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTTTCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATTCTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGG  >  NC_000913/602589‑603081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑