Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,742	G→A	G276D (GGT→GAT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,742	0	G	A	96.8%	193.3 / ‑4.0	63	G276D (GGT→GAT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (30/30);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCA  >  NC_000913/534499‑534987                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGt                                                                                                                                                                                                                                                <  2:215233/251‑1 (MQ=255)  aGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGGGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCTTGCACTGATGGCCGGGATGGGGGGTGCGCAAACCGCGGATCGTTACGGAAACGCAACGTGGCTGGCGTCTGACAT‑GGGGTTTGATATCgtt                                                                                                                                                                                                                                                >  2:29042/1‑248 (MQ=255)   gCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTaa                                                                                                                                                                                                                                              <  1:45738/251‑1 (MQ=255)         gAAAAGTTACTCCATGACGAACGTCGGGTGTTGGTTGCAGGGGGCGGGGAAATTACTGCTGACGCAGCTGCAATGTTACAACAGTTTTCTGAACTGACCAGCGATCCGGTGATACCAAAGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACAGATGGCCGGGATGGTGGGTCTGCAAACCACGCATCGTTACGGTAAAGCAAAGCTGATGGCGTCTGGCAT‑GGTGTTTAGTATCGGAAAcctctt                                                                                                                                                                                                                                        >  2:343700/1‑247 (MQ=255)             ggTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCt                                                                                                                                                                                                                                    <  2:116492/250‑1 (MQ=255)              gTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCt                                                                                                                                                                                                                                    <  1:109797/250‑1 (MQ=255)              gTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGGTTGCTGAACTGACCAGCGTGCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTa                                                                                                                                                                                                                                   <  1:453284/251‑1 (MQ=255)                 aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAAc                                                                                                                                                                                                                                 <  1:480577/250‑1 (MQ=255)                              cGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTc                                                                                                                                                                                                                   <  1:459862/251‑1 (MQ=255)                               gTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCg                                                                                                                                                                                                                  >  1:465905/1‑251 (MQ=255)                                    gTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCgggc                                                                                                                                                                                                               >  2:389768/1‑247 (MQ=255)                                       aTTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCggg                                                                                                                                                                                                                >  1:428093/1‑244 (MQ=255)                                       aTTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGt                                                                                                                                                                                                                <  2:428093/245‑1 (MQ=255)                                         tgttgCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGaaa                                                                                                                                                                                                         <  1:254110/250‑1 (MQ=255)                                                   ggCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAgg                                                                                                                                                                                              <  1:101467/251‑1 (MQ=255)                                                        ggTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATAAACCGAAGGgcgc                                                                                                                                                                                          >  1:36205/1‑250 (MQ=255)                                                         gTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCACCGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCa                                                                                                                                                                                         >  1:222748/1‑250 (MQ=255)                                                           aattaatGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAAGGCGCaaa                                                                                                                                                                                       >  1:163193/1‑250 (MQ=255)                                                                    tGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCat                                                                                                                                                                              >  1:292299/1‑250 (MQ=255)                                                                      aCGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTAGCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatatt                                                                                                                                                                           <  2:91381/251‑1 (MQ=255)                                                                       cGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGTGTCTGCAAACCGCGCATCGTTACGGTAACGCCACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCCTACCGGCTCGTTAGAGACATCCACCGAAGGGAGCAAAATCGTTCatattt                                                                                                                                                                          >  1:298369/1‑250 (MQ=255)                                                                         cAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgat                                                                                                                                                                        >  1:341356/1‑251 (MQ=255)                                                                           gCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgatat                                                                                                                                                                      >  1:140673/1‑251 (MQ=255)                                                                              gCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgatattgg                                                                                                                                                                   >  1:113029/1‑250 (MQ=255)                                                                              gCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCatattgatatt                                                                                                                                                                     <  1:340980/249‑1 (MQ=255)                                                                                  tGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGcc                                                                                                                                                                <  2:9960/250‑1 (MQ=255)                                                                                    ttACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGAGCATCGTTAAGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTACTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGGAAGGCACAAAATCATTCCTTATGATATTGAGCCgcc                                                                                                                                                             >  2:308875/1‑249 (MQ=255)                                                                                       caacaGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGc                                                                                                                                                           >  1:482493/1‑250 (MQ=255)                                                                                           aGTTTGGTGAACTGACCAGCGTGCCGGTGATCCCAACGTTAATGGGCTGGGGCTGTATCCCGGACGATCAGGAAATGATGGCCGGGATGGGGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCAGATTGATATTGAGCCGACGCAAAtt                                                                                                                                                      <  2:175528/251‑1 (MQ=255)                                                                                             tttGCTGAACTGACCAGCGTTCCGGTGCTCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGAAAAATCGTTCATATTGATATTGAGCAGACGCAAATTgt                                                                                                                                                    >  1:360671/1‑250 (MQ=255)                                                                                                gCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcg                                                                                                                                                 >  1:131456/1‑251 (MQ=255)                                                                                                   gAACTGACCAGCTTTCCGGTGATACAAACGCTAATTGGCTGGGGCTGTATCCCGGACGATCATGAATTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCTGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTcgcg                                                                                                                                               <  2:184424/250‑1 (MQ=255)                                                                                                      cTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCt                                                                                                                                           >  2:106841/1‑251 (MQ=255)                                                                                                         aCCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGGGAAATACAACGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTAGCGTGCtgtg                                                                                                                                        >  2:189715/1‑251 (MQ=255)                                                                                                                            cAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGTTATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGTTCGCGGTAttttc                                                                                                                     >  2:447742/1‑248 (MQ=255)                                                                                                                                 cTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTg                                                                                                                 >  1:212563/1‑250 (MQ=255)                                                                                                                                         cgggggccgTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGGGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAg                                                                                                        <  2:450482/249‑1 (MQ=255)                                                                                                                                            gggctgTATCCCGGACGATCATGAACTGATGGTCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAgcgg                                                                                                     <  1:371180/251‑1 (MQ=255)                                                                                                                                                 gtttCCCGGACGATCATGAACTGATGGCCGGGATGTTGGGTCTGCAAACCGCGAATCGTTACGGTAACGCAACGCTGCTGGCGTCGGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGGCCGGATCTCGGTATTGTCTCTTATGCTAAAGCGGCGCTg                                                                                                <  2:247200/248‑1 (MQ=255)                                                                                                                                                     cccGGACGATCATGAACTGATGGCGGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTTATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGATCCTACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTAGTGTCTCTGATGCTAAAGCGGCGCTGaca                                                                                             <  2:499512/250‑1 (MQ=255)                                                                                                                                                       cGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACActg                                                                                          <  1:385152/251‑1 (MQ=255)                                                                                                                                                         gACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACActgct                                                                                        >  1:385983/1‑251 (MQ=255)                                                                                                                                                                   aaCTGATGGCCGTGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTATAGCGGCGCTGACACTGCTGGTTGAAGTg                                                                              <  1:355573/251‑1 (MQ=255)                                                                                                                                                                          ggCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAg                                                                        <  2:79459/250‑1 (MQ=255)                                                                                                                                                                                   ggtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCaa                                                               <  1:330553/250‑1 (MQ=255)                                                                                                                                                                                    gtggGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCaaaa                                                             <  2:113029/251‑1 (MQ=255)                                                                                                                                                                                       ggTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGTTCTCGGTTTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGGAGTGGCGCAGGAGAGGCAAAAAGc                                                          >  2:413642/1‑251 (MQ=255)                                                                                                                                                                                        gTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGGGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAAAACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCAGACGCAGATTGGTCGAGGGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAACAGGAGCCTACACTGCTGGTTGAAGTGGCGCGGGAGGTGGAAACAGCg                                                         >  2:365881/1‑251 (MQ=255)                                                                                                                                                                                         tCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCgg                                                        >  1:192501/1‑251 (MQ=255)                                                                                                                                                                                            gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCggg                                                       <  1:12616/249‑1 (MQ=255)                                                                                                                                                                                            gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGgt                                                      <  1:106841/250‑1 (MQ=255)                                                                                                                                                                                            gCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCGCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCgg                                                        <  1:313945/248‑1 (MQ=255)                                                                                                                                                                                             aaaaCCGCGCATCGTTGCGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGCAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGCGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCCGGAGATGCAAAAAGCGGgtcg                                                    >  1:12543/2‑251 (MQ=255)                                                                                                                                                                                                aCCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCACAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATTCTAAAGCGGCGCTGACACTGCTGGTTGACGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCt                                                 >  1:296960/1‑251 (MQ=255)                                                                                                                                                                                                  cgcgCATCGTTACGGTAACGCCACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGGTATTGAGCCGGCGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACCCTGCTGGTTGCAGTGGCGCAGgagtgccacaaagaggtgcgtctgc                                               >  2:217133/1‑229 (MQ=255)                                                                                                                                                                                                  cgcgCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCATTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACGGCTGGTTGAAGGGGCGCAGGAGATGCAAAAAGCGGGTCGTCttc                                               >  2:306085/1‑249 (MQ=255)                                                                                                                                                                                                      cATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCGGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCgtg                                           <  1:413642/251‑1 (MQ=255)                                                                                                                                                                                                            tACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGCCACTGCTGGTTGAAGTGGCGCAGGGGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTa                                      >  2:138074/1‑250 (MQ=255)                                                                                                                                                                                                                             gctgGCGTCTGACAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACt                    >  1:199289/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggggcTGACATTGGTGTT‑GATATCGGTAACCGTTTTGCTAACCGTCCTACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAAGCGTTCATATTGAAATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCGCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCGTCCGTGTCGTAAAGAATGGGTCGCCGACTGCca                <  2:171154/247‑1 (MQ=255)                                                                                                                                                                                                                                         cAT‑GGTGTTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGCCTGCCAGCAGCGt         >  1:21163/1‑250 (MQ=255)                                                                                                                                                                                                                                              gtgtTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACg    <  1:363252/251‑1 (MQ=255)                                                                                                                                                                                                                                                gtTTGATATCGGTAACCGTTTTGCTAACCGTCATACCGGATCGGAAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGAAGCACATTGGTCGCGTGCTGTGTCCGGATCTCGAAATTGTCTCTGATGCTCAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAACGCGGGTCGTCTGCCGAGTCGTAAGGAATGGGTCGCCGACTGCCACCAGCGCAAAccgc  >  1:31573/1‑248 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGTCTGCAAACCGCGCATCGTTACGGTAACGCAACGCTGCTGGCGTCTGACAT‑GGTGTTTGGTATCGGTAACCGTTTTGCTAACCGTCATACCGGCTCGGTAGAGAAATACACCGAAGGGCGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCA  >  NC_000913/534499‑534987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑