Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 514,434 C→T G142G (GGG→GGA)  ybbJ ← NfeD‑like family protein YbbJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913514,4340CT95.2% 185.6 / ‑2.8 63G142G (GGG→GGA) ybbJNfeD‑like family protein YbbJ
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (39/21);  total (40/23)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGC  >  NC_000913/514188‑514679
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
cTGGTGAATCTGTTTAACGACCCGCAGCGGCTCAGCGCCGCCGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGCCACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAACGCCGCCTGCCACATCATCGAAAATCCCTCCGCCTCCTGTCATCA‑CCGGCAGGGGGTATTCCGACGCGACGCCTCCGTCATGTAGGGTTATTCCtt                                                                                                                                                                                                                                                    >  2:394014/1‑251 (MQ=255)
  ggTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCt                                                                                                                                                                                                                                                  <  1:329896/251‑1 (MQ=255)
      aaTCTGTTTAACGACGCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGAGCGAACGACACATTGAGGAGCTGCATGCCATGCCCGACCAGCTGCTGGCGCTGGCGAATGCCTGCCCTGGCGAAGTCAGCGGCGACTGCCCGATTATCGGAAATCTCTCCGGCTGCTGTCATCAGCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTAATTCCTTCTATCg                                                                                                                                                                                                                                              <  1:357143/251‑1 (MQ=255)
         ctgattAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGGTATGTAGGGTTATTCCTTCTATCGCa                                                                                                                                                                                                                                            >  2:197764/5‑250 (MQ=255)
           gTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATg                                                                                                                                                                                                                                         >  1:287970/1‑251 (MQ=255)
             ttAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGAc                                                                                                                                                                                                                                       >  1:412165/1‑251 (MQ=255)
                  gACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCa                                                                                                                                                                                                                                   >  2:411705/1‑250 (MQ=255)
                    cccGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACa                                                                                                                                                                                                                                <  1:193332/251‑1 (MQ=255)
                              cacaGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTg                                                                                                                                                                                                                      >  1:393661/1‑251 (MQ=255)
                                caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGc                                                                                                                                                                                                                     >  1:147168/1‑250 (MQ=255)
                                caGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCa                                                                                                                                                                                                                    <  2:356236/251‑1 (MQ=255)
                                     ccGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCgcg                                                                                                                                                                                                               >  1:494629/1‑249 (MQ=255)
                                       gACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCg                                                                                                                                                                                                                 >  1:170891/1‑247 (MQ=255)
                                         cGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATcc                                                                                                                                                                                                           >  2:102904/1‑251 (MQ=255)
                                          gTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCt                                                                                                                                                                                                          >  1:196010/1‑251 (MQ=255)
                                             aaaCGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGCTATGGAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGAACCGCGATCCTCGc                                                                                                                                                                                                       >  2:376894/1‑251 (MQ=255)
                                                 ggCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATGGAGGAGCTTGAAGCCATGCGCGACCAGCTGCGGGCACGGGCGAATGCCTGCCCGGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCGCCGGCTGCGGTCATCATCGGGCAGGGTG‑AGTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGgc                                                                                                                                                                                                   <  2:260870/251‑1 (MQ=255)
                                                    gcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATGGAAAATCTCTCCGGCTGTGGTCCTCTTGGGGACGGGTG‑ATTAAGACGAGACGGCTCGGATATGAAGGGTTATTCTTTCTATCGCATAGCCTTCAACCTGGGTACCTGCACCG‑GATccctccctgccctc                                                                                                                                                                                               >  2:85456/1‑239 (MQ=255)
                                                    gcACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCt                                                                                                                                                                                                >  1:489238/1‑251 (MQ=255)
                                                     cACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTTCCGGCAACGAGATCCTCGCTGgcgcgg                                                                                                                                                                                               >  2:350449/1‑249 (MQ=255)
                                                          tGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGATGCTGGCACTGGCGAATGCCTGCACTGGCGATGACAGCGCCGAATGCTCGCTTATCGCAAATCTCTCCGGCTGCTGTCATCATAGGGAAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCAAGATCCTCGCTGGCGCTAACAgg                                                                                                                                                                                          >  1:460152/1‑251 (MQ=255)
                                                            gagaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGCCCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGAAATGACTTCCACATGCGTACCTGCACCGAGATCCTCGCTGGCCCTGACCGGcc                                                                                                                                                                                        >  2:471466/1‑251 (MQ=255)
                                                            gagaAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGcc                                                                                                                                                                                        <  1:186199/251‑1 (MQ=255)
                                                                              gAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCACTGACTTCAACATGCGTACCTGCCCCGAGATCCTCGCAGGCGCTGACCGGCC‑CTGAACTGTCACcgccgc                                                                                                                                                                     >  2:163553/1‑251 (MQ=255)
                                                                                      caTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATg                                                                                                                                                              >  1:307069/1‑250 (MQ=255)
                                                                                      caTTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATg                                                                                                                                                              >  1:84111/1‑250 (MQ=255)
                                                                                         tGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGAcc                                                                                                                                                           <  1:157118/250‑1 (MQ=255)
                                                                                                cTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGGTATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGCGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCgg                                                                                                                                                   >  2:331150/1‑250 (MQ=255)
                                                                                                     aTCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGAcc                                                                                                                                              <  1:249492/251‑1 (MQ=255)
                                                                                                                  cAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATCTGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGCGATCCCCGCCGGCGCTGACAGGCC‑CTGAACTGTCACCGACCCGCATATGACCGCGCCCGATGCCCCGCCGAGATTccc                                                                                                                                 >  2:245502/1‑250 (MQ=255)
                                                                                                                       gctgGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGca                                                                                                                              <  1:247390/249‑1 (MQ=255)
                                                                                                                           gCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTAGCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTTCAGCACAAAAc                                                                                                                        <  2:439878/251‑1 (MQ=255)
                                                                                                                            cACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACg                                                                                                                       <  1:182794/251‑1 (MQ=255)
                                                                                                                             aCTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGGTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACACGCCTATGACCCCGCCCGGTGCCCCGCCGAGATCCCAGCACAAAACgc                                                                                                                      >  2:208619/1‑250 (MQ=255)
                                                                                                                              cTGGCGAATGCCTGCCCTGGAGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTATCCGCCCGGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAACACGTc                                                                                                                     >  2:486011/1‑251 (MQ=255)
                                                                                                                               tGGCGAATGCCTGACCTGGCGATGACAGCGCAGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACCAACCGTCg                                                                                                                    >  1:396068/1‑251 (MQ=255)
                                                                                                                                ggCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCg                                                                                                                    <  2:170891/250‑1 (MQ=255)
                                                                                                                                            cccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAgctgc                                                                                                       <  1:102904/251‑1 (MQ=255)
                                                                                                                                             ccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCCTATGACCGCGCCCGtt                                                                                                                                                  >  1:304597/1‑207 (MQ=255)
                                                                                                                                             ccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGTCCGCGCCCGtt                                                                                                                                                  <  2:304597/207‑1 (MQ=255)
                                                                                                                                             ccTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCGCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAgctgct                                                                                                      <  2:246382/251‑1 (MQ=255)
                                                                                                                                                ggggATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCAGCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGctgctg                                                                                                     <  2:505620/246‑1 (MQ=255)
                                                                                                                                                  cGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGAG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGCGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCcg                                                                                                 >  1:419693/1‑251 (MQ=255)
                                                                                                                                                      gACAGCTCCGCCTGCCCGATTATCGAAAATCTCTCCGGCGGATTGCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATTGAGGGTTATTCCTTCTACCGCAATGACTTCACCATGCGTACCTGCACCGAGATCCTCGCTGGCGTTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAACCGTCGGCCAATCAGCTGCTGCCCGCGCt                                                                                             <  2:448781/251‑1 (MQ=255)
                                                                                                                                                          gcgcCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGtt                                                                                         <  1:331150/251‑1 (MQ=255)
                                                                                                                                                              cGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                      >  2:359838/1‑250 (MQ=255)
                                                                                                                                                                   gccctactATCGAAAACCTCTCCGGCTGCTGTCATCATTGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGGAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCCTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCTACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGAcc                                                                                <  2:426075/244‑2 (MQ=255)
                                                                                                                                                                      cGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATc                                                                             <  1:4501/251‑1 (MQ=255)
                                                                                                                                                                       gATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGCCGAGACGGCTCGGCTATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGCCAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCCGCACAAACCGTCGGCCAATCAGCTGCTGCCCTCGCTGGGTTACATGACTATCg                                                                            >  2:2860/1‑251 (MQ=255)
                                                                                                                                                                          tATCGAAAATTTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAAGCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCt                                                                          <  2:374214/250‑1 (MQ=255)
                                                                                                                                                                                      ctcCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTcg                                                             >  1:81308/1‑251 (MQ=255)
                                                                                                                                                                                        ccGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTcg                                                             <  1:411705/249‑1 (MQ=255)
                                                                                                                                                                                        ccGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGCCGGCCAAACAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTcgcg                                                           >  2:349222/1‑251 (MQ=255)
                                                                                                                                                                                          ggCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGAACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCgcgc                                                          >  1:238437/1‑250 (MQ=255)
                                                                                                                                                                                            ctgctgTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCAcc                                                       >  1:97898/1‑251 (MQ=255)
                                                                                                                                                                                                                     aTTAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCcaccac                               >  1:180548/1‑251 (MQ=255)
                                                                                                                                                                                                                      taaaGACGAGTGGGCTGGGATATGTAGGGTTTTCCCTCCTACCGAAATGACTCCAACATGCGTACCTGCACCGAGATCCTCGCGGGCCCTGA‑AGGCCCATAACCTGTCCCCGACGCGCATATGCCCGCGCCCGTTGACCGGCGGATATCCCAGCAAAAACCGCCGGCCAATCAGCTGCTGCCCGCGTTGGTTAAAATGACTATCGCTGTTTTTTTGTTCGCCCTCCCGCCGCGACAACCATTTCcaccac                               <  1:292011/248‑1 (MQ=255)
                                                                                                                                                                                                                       tAAGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCcaccac                               >  1:472374/1‑249 (MQ=255)
                                                                                                                                                                                                                        aaGACGAGACGGCTCGGATATGTAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGc                            >  1:2506/1‑251 (MQ=255)
                                                                                                                                                                                                                                acgGCTCGGGTATGTAGGGGTCTTCCTTCTATCGCCCTGACTTCCACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCCGCGGAGATTCCCGCACCACACGTCGGCCCATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGGTTTTGTTCGCGCCCCCGCCGCGCCAACCATTTCCCCCCCAGCCAggcggc                    >  2:284165/1‑251 (MQ=255)
                                                                                                                                                                                                                                       ggATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACCACCATTTCCACCACAGACAGGCGTCGagccgc             >  2:1937/1‑248 (MQ=255)
                                                                                                                                                                                                                                              tAGGGTTATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGTGATCCTCGCTGGCGCTGACAGGCA‑ATGAACTATCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCCTTTCCACCACAGCCCGGCGGCGAGCAGCGTCAgg       >  1:241334/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   ttATTCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGc  >  1:26943/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
CTGGTGAATCTGTTTAACGACCCGCAGCGGCACAGCGCCGACGTCAAACGGCGCACGCTGGAGAAGGTGGCGGAGATCGAACGACACATTGAGGAGCTGCAATCCATGCGCGACCAGCTGCTGGCACTGGCGAATGCCTGCCCTGGCGATGACAGCGCCGACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTG‑ATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCC‑ATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGC  >  NC_000913/514188‑514679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑