Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,622	C→T	T168I (ACC→ATC)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,622	0	C	T	95.1%	102.0 / NA	42	T168I (ACC→ATC)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/17);  minor base A (1/1);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGTTC  >  NC_000913/495388‑495866                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      tGATTGCCCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCCGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAACAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACCTCCTCAAAGAAGATCGGGGt                                                                                                                                                                                                                                       >  1:59518/1‑251 (MQ=255)       aCCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGa                                                                                                                                                                                                                                  >  1:29440/1‑250 (MQ=255)         cATCTGGGGACTATCGCTAACTCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGATGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACACAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGACGTCTTCTGGGAATCGGCAGGCGGAGGGAAATACACCAGTGGAGGCAGCAACAAAGAAGATCGTGGGACTTAAAt                                                                                                                                                                                                                               >  2:478356/1‑251 (MQ=255)                       cGCTAAATCCGGTACCAAATCAGTCCTCGAATCCCTGGGGTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTg                                                                                                                                                                                                                  <  2:182920/250‑1 (MQ=255)                          tAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAACATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATAAACCGTTGCCGACATCATCAAAGAAGATCGTGGAACTGAAATACCCCTGCATCTGCGTg                                                                                                                                                                                                              >  1:320897/1‑251 (MQ=255)                            aaTCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGa                                                                                                                                                                                                             >  1:226437/1‑250 (MQ=255)                                         aTCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGAGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGAACTGAAATAACCCTGCATCTGCGTGAAGGCGAAGACGAGt                                                                                                                                                                                               >  1:324739/1‑251 (MQ=255)                                           cATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGGTTCTACTCTGCGTTTAACGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCTCCCTGCATCTGCGTGAAGTCGAAGACGAGtt                                                                                                                                                                                              <  2:94921/250‑1 (MQ=255)                                                     aTCCCTGGGTTCGTACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACt                                                                                                                                                                                   <  2:412905/251‑1 (MQ=255)                                                                cTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTCTCGTGGCCGACAAAGTGACCGTGCGTACTCGCGAGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGAGAATAACCCGTTGCCGGAATCATCAAAGAAGATCGTGGTACTGAAATCACCCCGCTTCTGCGTGATGCCGAAGACGAGTTCCTCGATGACTGGCgcgtgcgg                                                                                                                                                                        >  1:45038/1‑250 (MQ=255)                                                                  gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCgcgtgcg                                                                                                                                                                         <  1:114829/248‑1 (MQ=255)                                                                  gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCCGCCTCTTCGGGAAGGAGGAGACGGATTCCTCGCTGACTGGCgcgtgggtgc                                                                                                                                                                      >  2:202176/1‑246 (MQ=255)                                                                  gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGAGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAAAGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATAAACCGTTGCCAACATCTTCAAAGAAGCACGTGGTACTGAAATCACCCTGCCTCTGCGTGAAGGCGAACACGAATTTCTCGATGAcagggacgggagttt                                                                                                                                                                      >  2:361748/1‑237 (MQ=255)                                                                  gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTGTGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAAGCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCGCGATGACTGGCGCGTGCGTTc                                                                                                                                                                      <  2:195292/251‑1 (MQ=255)                                                                                          aTCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTTAAGGCGAAGACGAGTTCCTCGATGACTGGCgcgtgc                                                                                                                                                                          <  1:128487/223‑1 (MQ=255)                                                                                                  gTTTGGTGTTGGTTTCTACACTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTCCTCGCGCGGCAGGCGAAAAACCAGAAACTGGCGTCTTCTGGGAATAGGCTGGCCAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATAACCCTGCATCTGCGTGAAGGCGACGACGAGTTCCTCGATGACTGGCGCGTGCGTTTCATCATCAGCCACTACTCAGACCATATCgcgc                                                                                                                                      >  1:492368/1‑251 (MQ=255)                                                                                                     tGGTGTTGGTTTCTTCTCGTCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCAGATCGCGCTg                                                                                                                                    <  2:460438/250‑1 (MQ=255)                                                                                                            ggTTGCTACTCTGCGTTTATCTGGGCCGACAAAGAGACCGTGCGGACGCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAGCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGGGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCAGCATCAGCAAATACGCCGACCATATCGCGCTGCCGGTaga                                                                                                                            <  2:380848/251‑1 (MQ=255)                                                                                                                        gCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAAcg                                                                                                                <  2:211484/251‑1 (MQ=255)                                                                                                                                  tGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGaa                                                                                                       >  1:177381/1‑250 (MQ=255)                                                                                                                                       gACAAAGTGACCGTGCGTACTCGCTCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACgg                                                                                                 <  2:402205/251‑1 (MQ=255)                                                                                                                                                 ccGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGACGAGAAAGACGGCGAAACCGtt                                                                                       >  1:334469/1‑251 (MQ=255)                                                                                                                                                              cgcgGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCAACAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTGCGTTAAGGCGAAGACGAGTTCCGCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCTTTAGCTCCTGGgaga                                                                          <  2:20883/251‑1 (MQ=255)                                                                                                                                                                 ggaaGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACAGCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTGCCTCGATGACTGGCGCGTGCGATCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCGCCTGGGAGaaaa                                                                       <  2:121364/248‑1 (MQ=255)                                                                                                                                                                 ggCAGGCGAAAAACCATAAACTGGCGTCTTCTGGGAATCGGCTGGCTAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGaaaa                                                                       <  2:410573/251‑1 (MQ=255)                                                                                                                                                                          aaaaCCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGAGCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACCCCGACCATCTCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAACCCTTTATCTCCTGGGAGAAAAT‑CAACAAAg                                                             <  1:101028/251‑1 (MQ=255)                                                                                                                                                                                aGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCTTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAgg                                                       <  2:206894/251‑1 (MQ=255)                                                                                                                                                                                    aaTGGCGTCTTCTGGGAATCGGCTGGCGAAGGAGAATACACCGTTGCCGCCATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGCGATCGAAAAACGCGAAGAGAACGACGGCGAAACCGTTATATCCTGGGAGACAAT‑CCACAAAGCGCAGgcgcg                                                   >  1:476369/1‑250 (MQ=255)                                                                                                                                                                                     aTGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCtg                                                  <  2:106661/251‑1 (MQ=255)                                                                                                                                                                                       ggCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCGCCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCtgt                                                 <  2:123984/250‑1 (MQ=255)                                                                                                                                                                                                   gAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGGGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACa                                     >  1:112994/1‑250 (MQ=255)                                                                                                                                                                                                   gAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGATGGTAGAGATCGAAAAACGCGAAGAGAAAGACGTCGAAACCGTTATCTCCTGGGAGGAAAT‑CAACAAAGCGCATGAGCTGTGGACTCGTAACa                                     >  1:110960/1‑250 (MQ=255)                                                                                                                                                                                                     aTCGGCTGGCGAAGGTGGATACAACGTTGCCGACATCATCAAAGAAGATCGGGGTAATGCAATCACCCTGCATCCGCGTGGAGGCGAAAAAGAGTTCCCAGATGCCTGGCGCGTGCGTTCCCTCATACGCAAATACTCCGACCCCATAGCCCTGCAGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAAACGTTATCTCCTGGGGGAAAATACACCAAA‑AGCAGGCGCTGAGGAccctgaacaaa                                   >  2:464536/1‑240 (MQ=255)                                                                                                                                                                                                         gCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCgg                               >  1:426402/1‑250 (MQ=255)                                                                                                                                                                                                              cGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTTGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTGCCATCATCAGCAAATACGCCGACCATATCGCGCTGCCTGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCa                         <  1:36202/251‑1 (MQ=255)                                                                                                                                                                                                               gAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCAc                        >  1:98606/1‑251 (MQ=255)                                                                                                                                                                                                                     gAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGCAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGGCGGAAATCACCTATg                   >  2:67842/1‑250 (MQ=255)                                                                                                                                                                                                                           acCGGTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATATCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGAATCGTAACAAGTCGGAAATCACCGATGAAGAGt             >  2:416289/1‑250 (MQ=255)                                                                                                                                                                                                                              gTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACa          >  1:222731/1‑250 (MQ=255)                                                                                                                                                                                                                                tGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGGGGACTCGTAACAAGTCGGAAATCACCGCTGAAGGGAACaaa        >  2:390062/1‑250 (MQ=255)                                                                                                                                                                                                                                  ccGACAACATCAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTGCGTGAAGGCGAAGACGAGTTCCTCTATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAATAGAAAGACGTCGAAACCGTTATCTCCTGGGAGAAAAT‑CATCAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAagtg     <  1:331051/251‑3 (MQ=255)                                                                                                                                                                                                                                     aCATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGTTc  >  1:347309/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      TGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAAT‑CAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAGTACAAAGAGTTC  >  NC_000913/495388‑495866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑