Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	341,180	C→T	T19I (ACC→ATC)	yahJ →	amidohydrolase family protein YahJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	341,180	0	C	T	97.6%	117.5 / NA	41	T19I (ACC→ATC)	yahJ	amidohydrolase family protein YahJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (23/17);  minor base G (0/1);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCACCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCACGGCGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGAC  >  NC_000913/340939‑341424                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCCGAGCGGTAAGATGGTCATcgccgccgc                                                                                                                                                                                                                                             >  2:471888/1‑251 (MQ=255)     ccaTGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCAAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTg                                                                                                                                                                                                                                         <  1:278484/251‑1 (MQ=255)             cGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCACCCATCATCGCCAGCCTGACATTTTTCGAACAAGGCGGCAAAGAAGTGCTTATCACCACGCCGGAATGCCTGCCTGCGTCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTAc                                                                                                                                                                                                                                 >  2:330012/1‑251 (MQ=255)              gCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGCATGCCTGCCTGCGGCGCTGC‑GCGGCGAAATG‑GGCACTCATATTATTACAACGTAAGGGCGTCAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTCAGCCACAGCGGTAAGATGGTCATCGCAGCCGCGCTGTTTGGtgcc                                                                                                                                                                                                                                >  2:267422/1‑248 (MQ=255)                tataTGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACctc                                                                                                                                                                                                                              >  1:311941/1‑251 (MQ=255)                  tatGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCtg                                                                                                                                                                                                                            >  1:151621/1‑251 (MQ=255)                       aGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCg                                                                                                                                                                                                                       >  1:24732/1‑251 (MQ=255)                                             cagcaTGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTg                                                                                                                                                                                                 >  1:423191/1‑251 (MQ=255)                                                 aTGTTGCCAAAAATTATCCCAAGCCAGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCCCCACGCCGGAAGGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GTCAATCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCGGGCAc                                                                                                                                                                                             <  2:341269/251‑1 (MQ=255)                                                     tGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCt                                                                                                                                                                                          >  1:100618/1‑250 (MQ=255)                                                         aaaaaTCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCCCCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGtt                                                                                                                                                                                                                                       <  1:374152/201‑1 (MQ=255)                                                         aaaaaTCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGtt                                                                                                                                                                                                                                       >  2:374152/1‑201 (MQ=255)                                                                      agcctgcaagtgttAGCACATGGCTGCAAAGAAGGGATTACTACCCGGACGGAATGCCGGCCTGCGGCG‑TGCCGCGGCGAA‑CGCGCCACTAATATTATTAAAACGTAAGGAAGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGACCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGaacac                                                                                                                                                                        <  2:274246/239‑2 (MQ=255)                                                                         cTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAacac                                                                                                                                                                     >  1:23603/1‑251 (MQ=255)                                                                                   gaGAACAAGGGGGCAAAGAAGTGATTATCACCACGCGGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAACCG‑GGCACTCATATTATTAAAACGTAAGGACGGAAGAGGAAAGAAAGAAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAAt                                                                                                                                                            <  1:435712/249‑1 (MQ=255)                                                                                     gAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCAc                                                                                                                                                         >  1:170081/1‑251 (MQ=255)                                                                                                    gaagTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTCAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGCGCCGCTCGCCCATGCGGCGGGAGCTGGCCCCCTAAACTGCGAAGCGAACAACACCCTGAAAACCACTGACCCGCAttcct                                                                                                                                           >  2:264030/1‑247 (MQ=255)                                                                                                      agTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCCTTACTAtc                                                                                                                                        >  1:11879/1‑251 (MQ=255)                                                                                                          aTTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCg                                                                                                                                     >  1:321450/1‑250 (MQ=255)                                                                                                             atccccacGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATa                                                                                                                                  <  1:120563/246‑1 (MQ=255)                                                                                                             aTCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCAGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATa                                                                                                                                  >  1:270552/1‑250 (MQ=255)                                                                                                                 ccacGCCGGAAGGCCTCCGTGCGGCGCTGC‑GCGGCGAAAGG‑GGCACCCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGtg                                                                                                                             <  2:294459/251‑1 (MQ=255)                                                                                                                       ggcaatgctggcctgaGGCTCTGC‑GAGGCGAAAGT‑GACTCTCATATTATAAAAGTGTAAGGACGAAAGAGAAAAGAAGGAAAAGGCCGGCGTGAATTTCGGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGTGAAGCGAACAACGCCATGAAAAGCACTGACCCGCATTAGTATCTCGATAACGTGctgctg                                                                                                                       <  2:22786/241‑1 (MQ=255)                                                                                                                           aTGCCTGCCTGCGGCGCTGG‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGaaa                                                                                                                   <  1:487213/251‑1 (MQ=255)                                                                                                                              cctgcctgCGGCCCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCg                                                                                                                <  2:24732/251‑1 (MQ=255)                                                                                                                                 gcctgcGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGACACCCTAAACTGCGACGCGAACAACACACTGAAAATCCCTGACCCGCATTACTATCCCGATAACTGGATACTGGAAACCGGtt                                                                                                             >  2:164360/1‑251 (MQ=255)                                                                                                                                 gcctgcGGCGCTGC‑GCGGCGAAACG‑GGCACTCAAATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATATCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGt                                                                                                              <  1:458841/250‑1 (MQ=255)                                                                                                                                     gcggcgcggc‑gcggcGAAACG‑GGCCCTCATATTATAAAAACGTAAGGACGTAAGAGGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGtttt                                                                                                           <  1:109511/249‑1 (MQ=255)                                                                                                                                        ggtcTGC‑GCGGCAAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAGGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTa                                                                                                      <  1:52386/248‑1 (MQ=255)                                                                                                                                          gcTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTACGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCCTGCGGCGGTATCTTGCACCCTAAACTGTGAACCGAACAACACCATAAAAATCACTGACCCGCATTAATATATCGATAACGTGCTGCTGGGAACCGGATTTGATTACg                                                                                                    >  1:7632/1‑251 (MQ=255)                                                                                                                                             gc‑gcGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAACTGGACCCCTAAACTGCGAAGCGAACAACACCATGAAACTCACTGACCCGCATTACTATCTCGATAACGCGCTGCTGGAAACCGGTTTTGACTACGaa                                                                                                  >  2:85436/1‑250 (MQ=255)                                                                                                                                                                cacATATAATTAAAACGGAAGGCCGTAAGTTGAAAAAAAGCAAAAGCCGCCGTGAATTTCTGAGCCAGAGCGGGAAGATGGGCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGAGAACGTGCTGCTGTAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAg                                                                                <  2:270552/249‑1 (MQ=255)                                                                                                                                                                        attaAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCAc                                                                        >  1:403513/1‑251 (MQ=255)                                                                                                                                                                          taAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGGGCCGACCCGCACgg                                                                      >  2:110419/1‑251 (MQ=255)                                                                                                                                                                                             cctatcaaaagaaacatccgcctTGAATTTCTGAGCCAGAGCGGCAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGAGAACGGGCTGCTGGAAACCGGTGTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCACGGCGCGCCAGACCGTGgag                                                     <  2:357376/232‑1 (MQ=255)                                                                                                                                                                                             aTGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCAAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGGAGCGAACAACACCATGAAAATCACTGGCCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGATTTGACTAAGAAAATGGCGTGGCGGTGGAGAACCGGACGGGGGGCCAGAcggggg                                                       >  1:104723/1‑242 (MQ=255)                                                                                                                                                                                                    aaagCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGGAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCAACCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCACGGCGCGCCAggacgaggggagatcgg                                              >  1:357376/1‑233 (MQ=255)                                                                                                                                                                                                                 ccgttcaTTTCTGCGCCAGAGCGGTAAGATGGGCATCGCCGCCGCGCTGTCTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCGCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGGGCAGACCCGCACGGCGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCg                               <  2:341270/245‑1 (MQ=255)                                                                                                                                                                                                                   gTGAATTTCTGAGCCAGAGCGGTAAGTTGGTCAGCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACGGCTAAGCGAACAACACCATGAAAATCACTGACCCGCAGTACTAGCTCGATAACGTGCTGGTGGAAACCGGTTTTGACTACGAAAATGGCGTTGCGGTGCAGACCCGCACGGCGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGcc                             <  1:231018/251‑1 (MQ=255)                                                                                                                                                                                                                        tttCTGAGCCAGAGCGGTAAGATGGTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGAACCGCACGGCGCGCCCGACCGTGGAGATTCAGGACGGCAAAAATGTCGCCCTgcg                        >  1:97464/1‑251 (MQ=255)                                                                                                                                                                                                                                               ggTCATCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCCCCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCACGGCGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGAc  <  2:49774/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GCCACCATGACCCGCTATATGCAGGAAGGGCATTTCCCGCCCGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACATTTTTAGAACAAGGCGGCAAAGAAGTGATTATCACCACGCCGGAATGCCTGCCTGCGGCGCTGC‑GCGGCGAAACG‑GGCACTCATATTATTAAAACGTAAGGACGTAAGATGAAAGAAAGCAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTCACCGCCGCCGCGCTGTTTGGTACCTCTGTGCCGCTCGCCCATGCGGCGGTAGCTGGCACCCTAAACTGCGAAGCGAACAACACCATGAAAATCACTGACCCGCATTACTATCTCGATAACGTGCTGCTGGAAACCGGTTTTGACTACGAAAATGGCGTGGCGGTGCAGACCCGCACGGCGCGCCAGACCGTGGAGATTCAGGACGGCAAAATTGTCGCCCTGCGCGAGAACAAGCTGCATCCGGAC  >  NC_000913/340939‑341424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑