Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913321,2600AT100.0% 78.7 / NA 26intergenic (+179/‑348)rclR/ykgEDNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/12);  total (14/12)

CCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTA  >  NC_000913/321039‑321502
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
cccGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGAtttt                                                                                                                                                                                                                       >  2:389352/1‑251 (MQ=255)
                          aaCAGCTTGCACCTTGAATAAATCCGCCAGAAACCAGGGCAAAGATAATCCGCATTTCGGGAGGTGTGAGCTTTTCATCTAGTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATATAATCCCGACTATTTCTTGATACGTGATTATTGGCGTTGATTTTTTTGTACACCATCAGTGTATATCTCa                                                                                                                                                                                             <  2:420419/250‑1 (MQ=255)
                              gCTTGCCCCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTTAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATaa                                                                                                                                                                                         <  2:68204/251‑1 (MQ=255)
                                               aaCCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGct                                                                                                                                                                        >  1:233870/1‑251 (MQ=255)
                                                                   ccgatcatcctccttCCGGGATTTTTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTCGCTCTGAATAGGTATAAGGGATg                                                                                                                                                    >  1:157709/14‑251 (MQ=255)
                                                                      aTAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAg                                                                                                                                                 <  1:338812/251‑1 (MQ=255)
                                                                           ccGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATACGGGATGTAGCCAtt                                                                                                                                            >  2:117946/1‑251 (MQ=255)
                                                                             gCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCAtttt                                                                                                                                          <  2:17210/251‑1 (MQ=255)
                                                                                              aGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGAATAAGGGATGTAGCCATTTTTAATTGGATTTCTTATg                                                                                                                         >  2:161926/1‑251 (MQ=255)
                                                                                                                                     tttATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCACGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCAAAAAATGATCGTCGATTTCCCCATAAATTGTGAGCg                                                                                   >  1:366403/1‑250 (MQ=255)
                                                                                                                                            atTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCgaa                                                                           <  1:57731/251‑1 (MQ=255)
                                                                                                                                                         gACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTAGTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTa                                                              <  1:300248/251‑1 (MQ=255)
                                                                                                                                                                     gTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCAttt                                                  <  1:350369/251‑1 (MQ=255)
                                                                                                                                                                       ttttAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTg                                                 >  1:9564/1‑250 (MQ=255)
                                                                                                                                                                          tcaatagaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCACTGGATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCAATTTTAAATGGATTTATTATGCCAAAAATGATCGTAGATTACCCCATAAACTGTGAGCGATGCCAAAAAAAATAAAATTAGTTATGGCAttttacat                                             >  2:127770/3‑246 (MQ=255)
                                                                                                                                                                          taaataaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGt                                             >  1:54385/1‑251 (MQ=255)
                                                                                                                                                                             ataaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTg                                          <  1:290317/251‑1 (MQ=255)
                                                                                                                                                                                aaaaTGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATg                                                                                                                                    <  1:417045/158‑1 (MQ=255)
                                                                                                                                                                                aaaaTGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATg                                                                                                                                    >  2:417045/1‑158 (MQ=255)
                                                                                                                                                                                     gCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTCTAAggg                                                                                                                                                       >  1:83984/1‑134 (MQ=255)
                                                                                                                                                                                     gCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGGATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAggg                                                                                                                                                       <  2:83984/134‑1 (MQ=255)
                                                                                                                                                                                         aCTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGAtt                               >  1:482972/1‑250 (MQ=255)
                                                                                                                                                                                              aTTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCAtt                         <  1:389352/251‑1 (MQ=255)
                                                                                                                                                                                                           aataaaATCCCGACTATTTCTTGCTACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATg            >  1:341399/1‑251 (MQ=255)
                                                                                                                                                                                                               aaaTCCCGACTATTTCGTGATACGTGAGTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCAGAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGGTTGGATGAAAGATTATCATTTGTCATACAAATGAggg        <  2:345881/251‑1 (MQ=255)
                                                                                                                                                                                                                     cGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTTTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATCAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGgggggtt  >  2:403344/1‑250 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  
CCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTA  >  NC_000913/321039‑321502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑