Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	292,019	C→T	L174L (CTA→TTA)	yagJ →	protein YagJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	292,019	0	C	T	96.8%	190.4 / ‑1.8	63	L174L (CTA→TTA)	yagJ	protein YagJ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (27/34);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-01

GTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACT  >  NC_000913/291783‑292257                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               gtgACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATTGTTGTGGACCTGGTAGATCCCCTTAGCTTACATCTGGCTGAt                                                                                                                                                                                                                                  <  2:59218/251‑1 (MQ=255)    aCGAAAGAGGTACCTATCCGGCTCTGTTGAACCAGTGGGAAACCAAGGGTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTGTCTGGTATCGTAATCCACAGTACTCAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCAGGTAGATCTACATAGCTTACATCCGGCTGATGCt                                                                                                                                                                                                                               <  2:179/251‑1 (MQ=255)                   aTCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCtt                                                                                                                                                                                                                              <  1:14957/237‑1 (MQ=255)                   aTCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCtt                                                                                                                                                                                                                              >  2:14957/1‑237 (MQ=255)                      cGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCTCTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGa                                                                                                                                                                                                             <  1:164981/251‑1 (MQ=255)                           ctggtGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACtt                                                                                                                                                                                                          <  2:78448/245‑1 (MQ=255)                             gTTGAACCATTGAGAAACCAAGGTTTTTGATATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGATCAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTgcgc                                                                                                                                                                                                      <  1:21721/251‑1 (MQ=255)                                gAACCATTGGGAAACCAAGGTTTTTGCGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAACCTGGAAGGACTTGCGCtat                                                                                                                                                                                                   >  2:218032/1‑251 (MQ=255)                                    cATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACCGGGCCGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGCACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGATTACATCTGGCTGCTGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGc                                                                                                                                                                                               >  1:476805/1‑251 (MQ=255)                                     aTTGGGAAATCAAGGGTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGGAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGcc                                                                                                                                                                                              <  1:492941/251‑1 (MQ=255)                                          gAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAc                                                                                                                                                                                          >  1:128748/1‑250 (MQ=255)                                          gAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAc                                                                                                                                                                                          >  1:1978/1‑250 (MQ=255)                                              ccAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAAcatcat                                                                                                                                                                                     <  1:117110/251‑1 (MQ=255)                                               cAAGGTGTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCAtt                                                                                                                                                                                    <  1:343037/251‑1 (MQ=255)                                                            aTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAg                                                                                                                                                                       <  2:1978/251‑1 (MQ=255)                                                                  aCCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGcgaat                                                                                                                                                                 >  2:413790/1‑251 (MQ=255)                                                                           gAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGt                                                                                                                                                        <  1:413790/251‑1 (MQ=255)                                                                            aaGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATCGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGCATCGAATCTGTc                                                                                                                                                       >  1:127761/1‑251 (MQ=255)                                                                                    gCTTTCTGGTATCGTAAACCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGGTGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGaat                                                                                                                                                <  2:366858/250‑2 (MQ=255)                                                                                              aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAg                                                                                                                                                                       >  1:257812/1‑217 (MQ=255)                                                                                              aTCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAg                                                                                                                                                                       <  2:257812/217‑1 (MQ=255)                                                                                                cGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAAtt                                                                                                                                   >  1:329591/1‑251 (MQ=255)                                                                                                        acaGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAgtgt                                                                                                                           >  1:407191/1‑251 (MQ=255)                                                                                                         caGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTTGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGtt                                                                                                                          <  2:373051/251‑1 (MQ=255)                                                                                                             acacAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGAt                                                                                                                      <  1:303783/251‑1 (MQ=255)                                                                                                                         cgtcACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCCCATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGTTTTGAAACGGCAg                                                                                                          <  1:149600/251‑1 (MQ=255)                                                                                                                         cgtcACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGc                                                                                                            >  2:466347/1‑249 (MQ=255)                                                                                                                          gccACTGGGAATCGCTTATGATGAAGCTGAAAAGTAAAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAGTGGTTGTGGAACTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTAACAGGCGAATCGAATCTGTCGCCGCAGTAAAGGGTAAATTACGAGTGTTAGATTTGAACCGGccgg                                                                                                         >  2:288936/3‑248 (MQ=255)                                                                                                                             ccggggAATCGTTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTTCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGTCTTGCGCTATATGCCGAACATCATGCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGAtg                                                                                                      <  2:269679/248‑1 (MQ=255)                                                                                                                               tGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGAtgtg                                                                                                    >  1:466409/1‑251 (MQ=255)                                                                                                                                   aaTCGCTTAGGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAgg                                                                                                <  2:139614/251‑1 (MQ=255)                                                                                                                                          tATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGACCATCATTCTGATGCTTACAGGCGAATCGAATCTTTCGCCGAAGTAAAGGGGAAATTa                                                                                                                                  >  2:99920/1‑210 (MQ=255)                                                                                                                                          tATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTa                                                                                                                                  <  1:99920/210‑1 (MQ=255)                                                                                                                                             gTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGc                                                                                      >  1:180553/1‑251 (MQ=255)                                                                                                                                                   gCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGAAGGATGCTGTTGCTACCGc                                                                                >  2:265511/1‑251 (MQ=255)                                                                                                                                                      gAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGa                                                                             <  1:328101/251‑1 (MQ=255)                                                                                                                                                        aCAGTACAAGACTGTTCGTACCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTTCATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGATATATGCCGAACATCATTCTGATGCTTACAGGCGAATCTAATCTGTCGCCTAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTTCAGGATGCTGTTGCTACCGCTGaaa                                                                           <  1:403419/251‑1 (MQ=255)                                                                                                                                                                 gATTGTTAGTCCCGATTTCCTGTTCTTGGCCGAAAAGGATGGCAAAATGGTTGTGGACCTGGTAGATCAACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGATATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGAAAATTACGCGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACAGATGAAAATGCAGaa                                                                   >  2:434509/1‑250 (MQ=255)                                                                                                                                                                  aTTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAAc                                                                 <  2:123430/251‑1 (MQ=255)                                                                                                                                                                      ttCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGCACTTGCGCTATATGCCGAACATCATTCTGATGATTAAAGGCGAATCGAATCTGTCGCCGAGGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGGTGCTAACGCTGAAAATGCCGAAACGtt                                                              >  1:457262/1‑250 (MQ=255)                                                                                                                                                                       tCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCGAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTa                                                             <  1:310075/250‑1 (MQ=255)                                                                                                                                                                        cGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATTGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAATGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTGCCGCTGAAAATGCAGAAACGTTAt                                                            <  2:308877/250‑1 (MQ=255)                                                                                                                                                                        cGTCCCGATTTACTGTTCTTTGCCGAACAGGATGGCACAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTACATTCCGAGTGTTCGATTTGCAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTAtt                                                           >  2:444378/1‑251 (MQ=255)                                                                                                                                                                         gTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCAGTTGCTACCGCTGAAAATGCAGAAACGTTAttt                                                          >  2:251699/1‑251 (MQ=255)                                                                                                                                                                           cccGATTTCCTGTTCTTTGCCGAACATGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTtag                                                        <  2:160453/251‑1 (MQ=255)                                                                                                                                                                             cGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTtagt                                                       >  1:127844/1‑250 (MQ=255)                                                                                                                                                                               aTTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGATAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGc                                                    <  1:264266/251‑1 (MQ=255)                                                                                                                                                                                   ccTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGAc                                                <  1:353168/251‑1 (MQ=255)                                                                                                                                                                                          tttGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGGCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTTCAGGATGCTGTTTCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCtg                                          <  1:484752/250‑1 (MQ=255)                                                                                                                                                                                            tGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCtgat                                        <  2:128748/250‑1 (MQ=255)                                                                                                                                                                                              ccGAACATGATGGCAAAATGGTTGTGGACCTGGTAGATCCACAGAGCTTACGTCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAAACGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGAc                                     <  1:165820/251‑1 (MQ=255)                                                                                                                                                                                                          gaaaaaTGGTTGTGGACATGATAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGACGGACTAGCGATATATGCCGAACATCAGTATGATGCTTAAAGGCGCATCGAGTCTGACGCAGAAGTAAAGGGGAAATTAGGAGAGTTAGAGTTGAAACGGCCGGATGGGCAGGATGCTGTTGCACCCGCTGAAAATGCAGAAACGTCATTTAGTAGAGTGCTTGTTGATGACTATCactacttt                         >  2:419045/3‑244 (MQ=255)                                                                                                                                                                                                             aaaTGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCtat                       >  1:341002/1‑250 (MQ=255)                                                                                                                                                                                                              aaTGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAg                     <  1:109196/251‑1 (MQ=255)                                                                                                                                                                                                               aTGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGa                                                                                                                       >  1:164997/1‑152 (MQ=255)                                                                                                                                                                                                               aTGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGa                                                                                                                       <  2:164997/152‑1 (MQ=255)                                                                                                                                                                                                                          ccTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGAAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTa          >  1:468890/1‑250 (MQ=255)                                                                                                                                                                                                                            tGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAAtt               >  1:316201/1‑243 (MQ=255)                                                                                                                                                                                                                            tGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGGTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAAtt               <  2:316201/243‑1 (MQ=255)                                                                                                                                                                                                                            tGGTAGAACCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGATAAATTGCGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGAGGACTATCAGTAATCTATAGAAAATTGAGTAACg       >  2:373360/1‑251 (MQ=255)                                                                                                                                                                                                                             ggTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGGTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAAc        <  2:127844/249‑1 (MQ=255)                                                                                                                                                                                                                              gTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGaa     <  2:466409/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACt  >  1:448669/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               GTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACT  >  NC_000913/291783‑292257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑