Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,838	T→A	pseudogene (75/427 nt)	insN →	IS911A regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,838	0	T	A	95.2%	166.3 / ‑0.8	63	pseudogene (75/427 nt)	insN	IS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (36/23);  total (39/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

ATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTC  >  NC_000913/271599‑272082                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     atgttggcctttccacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGTACACACTCTGCCAtgt                                                                                                                                                                                                                                           <  1:279449‑M1/88‑1 (MQ=255)     tggcctttccacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGtt                                                                                                                                                                                                                                        >  1:317729‑M1/160‑250 (MQ=255)      ggcctttccacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggcaACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCCGGATAATCGGGAAACTCAAAGCGCATTATCCTGTGGTCACACTCTGCCATGtgttgg                                                                                                                                                                                                                                      <  1:270384‑M1/93‑3 (MQ=255)       gcctttccacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCgg                                                                                                                                                                                                                                     >  1:245938‑M1/158‑251 (MQ=255)        cctttccacaatgacaagatgggtcacacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCggg                                                                                                                                                                                                                                    <  2:417687‑M1/95‑1 (MQ=255)            tccacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGtt                                                                                                                                                                                                                                 >  1:293144‑M1/153‑250 (MQ=255)              cacaatgacaagatgggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCAt                                                                                                                                                                                                                              >  2:51665‑M1/151‑251 (MQ=255)                  atgacaagattggtcaaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCgca                                                                                                                                                                                                                          >  1:101230‑M1/147‑251 (MQ=255)                    gacaagatgggtcaaacaactgcgtgacgagcgtcagggcaaaacaccaaaagtctctccgataacaccagaacaaagcgaaatacggaagctgaggaaaaagctacaactcatttaaatggagaatgaaatattaaaaaggctACCGCGTTCTTGATGTCAGACTCCCTGAACAGTCCTCGATAAGCGCGAAACTCTGAGCGCATTATCCTGTGGTCACCCTCTGCCTTGTGTTCGGGGTtcatctcag                                                                                                                                                                                                                         >  2:174889‑M1/145‑246 (MQ=255)                                caaacaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATAc                                                                                                                                                                                                            >  1:449792‑M1/133‑251 (MQ=255)                                   acaactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagcgacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTgg                                                                                                                                                                                                         >  2:314314‑M1/130‑251 (MQ=255)                                     aactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCagcagctacagataatggc                                                                                                                                                                                                        <  2:280190‑M1/123‑10 (MQ=255)                                     aactgcgtgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGaa                                                                                                                                                                                                       >  1:280190‑M1/128‑251 (MQ=255)                                            tgatgagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgacatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCCCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCGGCTACAGATACTGGAAAAACcgg                                                                                                                                                                                                <  2:427201‑M1/131‑2 (MQ=255)                                             tCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCCCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTc                                                                                                                                                                                               >  1:251442/1‑251 (MQ=255)                                                gagcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCACGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCAGGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTg                                                                                                                                                                                            >  1:417687‑M1/117‑251 (MQ=255)                                                  gcgtcagggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGaa                                                                                                                                                                                          >  1:437738‑M1/115‑251 (MQ=255)                                                       agggcaaaacaccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCctgaaaaacc                                                                                                                                                                                     >  1:345417‑M1/110‑248 (MQ=255)                                                              aactccaaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcatggaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcagacggc                                                                                                                                                                              >  2:229638‑M1/103‑251 (MQ=255)                                                                    aaaagcctctccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctaaaaagcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcaaacggcagacgg                                                                                                                                                                        <  1:342424‑M1/155‑1 (MQ=255)                                                                              ccgataacaccagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTa                                                                                                                                                               >  1:53756‑M1/87‑250 (MQ=255)                                                                                      accagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTc                                                                                                                                                        <  1:60738‑M1/171‑1 (MQ=255)                                                                                      accagaacaaatcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTc                                                                                                                                                        >  2:60738‑M1/79‑249 (MQ=255)                                                                                         ggaacaaatcgaaatacgtacgctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCGGACTCCCTGAACAGTTCTCGATATTCGGGAAACTCAGAGAGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGCAAAACCGTCCTGAAGAACCAGCCGGCAGACGGGctgaattacgagatcaggt                                                                                                                                                    <  2:95146‑M1/175‑10 (MQ=255)                                                                                                atcgaaatacgtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctAACGCGCTCTTGATGTCAGACTCCTTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGTATTACCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGTAGTCAGGTACTtgagc                                                                                                                                            >  1:95146‑M1/69‑249 (MQ=255)                                                                                                  cgagatacgtaagatgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACACCCAGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCATTATCCTGTGGTAACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGCCGTGCTGCATTACGCAGTCAGGAACttgagct                                                                                                                                           <  2:75898‑M1/184‑4 (MQ=255)                                                                                                          gtaagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCAt                                                                                                                                  <  1:376622‑M1/193‑1 (MQ=255)                                                                                                          gtaagctgaggaaaaagctacaacgcattgaaagggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCTATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTGCGGGGTTCATCGCAGCAGCTACAGATTCTGGAAAAACCGTCCTTAAAAACCAGACGGCAGACGTGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCAt                                                                                                                                  >  1:463123‑M1/59‑251 (MQ=255)                                                                                                           taagctgaggaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATc                                                                                                                                 <  1:366332‑M1/194‑1 (MQ=255)                                                                                                                    gaaaaagctacaacgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCATGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGCGCTACATGGCATCAGCCAcggt                                                                                                                        <  1:304605‑M1/203‑2 (MQ=255)                                                                                                                       aaagctacaacgcattgacatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAACAACCAGACGGCAGCCGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGccacggatcg                                                                                                                     <  2:189247‑M1/206‑5 (MQ=255)                                                                                                                                acgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTAGCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCa                                                                                                            >  2:386843‑M1/37‑251 (MQ=255)                                                                                                                                 cgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAACCCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCa                                                                                                            <  2:248114‑M1/215‑1 (MQ=255)                                                                                                                                             gagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGcc                                                                                                <  1:149895‑M1/227‑1 (MQ=255)                                                                                                                                             gagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCca                                                                                               >  1:115820‑M1/24‑251 (MQ=255)                                                                                                                                               cATTGACAGATTGACTCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAACGCCCGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCAACGcccc                                                                                              >  2:48899/1‑248 (MQ=255)                                                                                                                                                     aatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcaga                                                                                                                                                                                  >  2:358349‑M1/16‑160 (MQ=255)                                                                                                                                                     aatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACcaga                                                                                                                                                                                  <  1:358349‑M1/145‑1 (MQ=255)                                                                                                                                                        attaaaaaggctACCGCGCTCTTGATGTCAGTCTCCCTGAACAGTTCGCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAAcc                                                                                    >  2:366332‑M1/13‑251 (MQ=255)                                                                                                                                                             aaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAACAACCGTCCTGAAACACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGAACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCgccccaatggaaacacgg                                                                                 <  2:216701‑M1/242‑9 (MQ=255)                                                                                                                                                              aaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCggaga                                                                              >  1:291973‑M1/7‑251 (MQ=255)                                                                                                                                                               aggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCAtcagccacggttcggccg                                                                                                                 >  2:157561‑M1/6‑207 (MQ=255)                                                                                                                                                               aggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCAtcagccacggttcggccg                                                                                                                 <  1:157561‑M1/210‑9 (MQ=255)                                                                                                                                                                   tACCGCGCTCTTGATGTCAGACTCCATGAACAGTTCTCGATAATCGGGAAAATCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGGTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAAACAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTAAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGGAACCCGGAgaggct                                                                          <  1:351208‑M1/249‑3 (MQ=255)                                                                                                                                                                     ccGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGACACTCAGAGCGAATTATCCTGTAGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCTTGAAAAAACAGACGGCAGACGGGCTGTATTCCGCAGTAAGGTACTTGCACTACATGGCATAAGCCACGGTTTGGCCGGAGCAAGAAGCATCGCCACACGGGCAACCCagagcggcttcc                                                                       >  2:60439/1‑243 (MQ=255)                                                                                                                                                                     ccGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCa                                                                                                                                <  2:228563/194‑1 (MQ=25)                                                                                                                                                                     ccGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCa                                                                                                                                >  1:228563/1‑194 (MQ=25)                                                                                                                                                                      cgcgCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTAcc                                                                       >  1:156408/1‑250 (MQ=18)                                                                                                                                                                          ctctTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATg                                                                  >  2:79961/1‑251 (MQ=18)                                                                                                                                                                               gATGTCAGACTCCCTGAACAGTGCCCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATgggacg                                                             <  1:427201/251‑4 (MQ=17)                                                                                                                                                                                     aGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCt                                                       >  1:66770/1‑251 (MQ=17)                                                                                                                                                                                                aCAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAggc                                            >  1:495618/1‑249 (MQ=14)                                                                                                                                                                                                    ttCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCAACAATGGCACACCGGAGAGGCTACCAGATGAGACGCTTGCTTACTGGCAggcaca                                         >  2:131604/1‑245 (MQ=14)                                                                                                                                                                                                      ctcGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATg                                       >  1:425928/1‑250 (MQ=14)                                                                                                                                                                                                                 ggAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCGGGCTCATGAAAGAGCTgggg                           >  2:280533/1‑251 (MQ=11)                                                                                                                                                                                                                   aaaCTCAGAGCTCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCt                         <  2:156408/251‑1 (MQ=11)                                                                                                                                                                                                                    aaCTCAGAGCGAATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAACACCGTACTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGAATCAGCCACGGTTCGGCCGGAGAAAGAAGCATCGCCACAATGGCAACCCGGAGCGGCTACCAGATGGGACGCTGGCTTGCTGGACGGCCCATGAAAGAGCTgggggtg                        >  2:459821/1‑248 (MQ=11)                                                                                                                                                                                                                     aCTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCGCATGAAAGAGCTGGGGCTgg                       >  1:143196/1‑251 (MQ=11)                                                                                                                                                                                                                         agagcgcATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTc                     >  1:481864/4‑249 (MQ=11)                                                                                                                                                                                                                           agcgcATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGCGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGACAgct                 >  1:177892/2‑249 (MQ=11)                                                                                                                                                                                                                            gcgcATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCATTCAGGTACTTGAGCTACATGGCAGCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAgctg                <  2:216174/251‑4 (MQ=11)                                                                                                                                                                                                                                 ttATCCTGTGGTCACACTCTGCCATGTGTTAGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGCAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCCCAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCCGGCTAATGCAAGAGCTGGGGCTGGtaagctggcagc           >  2:452652/1‑240 (MQ=11)                                                                                                                                                                                                                                          ggCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAAGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTc  <  2:251442/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     ATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGCAGCCGACTC  >  NC_000913/271599‑272082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑