Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	48,033	T→G	C89G (TGT→GGT)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	48,033	0	T	G	100.0%	194.5 / NA	59	C89G (TGT→GGT)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/34);  total (25/34)

GATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCTGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGC  >  NC_000913/47786‑48277                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTTTTGGTCTGGGAGCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCggtg                                                                                                                                                                                                                                                    <  2:369801/251‑1 (MQ=255) gATTCAGGCGCTGATTTATCTCGGGTCTGCAGCGCTGGTTGTACCCATGGCGGTCCGTCTTGGTCTTGGATCGGTACTTGGCTACCTGACCGCCGGCTGCATTATTGGCCCGTGGGGGCTTCGTCTGTTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGTTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCggtg                                                                                                                                                                                                                                                    <  2:110015/251‑1 (MQ=255)     cAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGgcg                                                                                                                                                                                                                                                 <  1:410872/250‑1 (MQ=255)                     gcGGTTCGGCAGCGCTGATGGTTCCCATTGCGGTAGGTGTTGGGCGGGGATCTGTACTGGGCTACCTGATCGTCGGCTGCATGAGTGGCCCTTGGGGGCTGCGACGGGTGGTCGATGCCGAATCTATTCTGCTCTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCGGTGGAAGATGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGagt                                                                                                                                                                                                                                <  2:488222/250‑3 (MQ=255)                             gCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGACGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGcggcgg                                                                                                                                                                                                                        >  1:128779/1‑251 (MQ=255)                                gcgcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCct                                                                                                                                                                                                                     <  1:249859/251‑1 (MQ=255)                                     gatgtgaCCCATTGCGTGACGTCTTGGTCTGGGATCGGTACTTGGCTGCCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAAGCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCctgctg                                                                                                                                                                                                                 <  2:209062/244‑1 (MQ=255)                                     gATTGTTCCCATTGCTGTTCGTCTTGGTCTGTGGTCGGTACTTGGCTACCTGATCGGCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCTCTTTGACGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCctgct                                                                                                                                                                                                                  <  2:128779/249‑1 (MQ=255)                                     gATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCGGCGTGCGGCAGGGTTCGGCGGTGGCGCATTGCAGAGGGTGATTTGcggcgggccggtgg                                                                                                                                                                                                                >  2:21750/1‑243 (MQ=255)                                      aTTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGGGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGGGGCGCATTGCAGCTGGGGATTTGTGGCGGCCTGCTggg                                                                                                                                                                                                               >  2:202943/1‑251 (MQ=255)                                      aTTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTggg                                                                                                                                                                                                               >  1:357264/1‑251 (MQ=255)                                                    ggTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGt                                                                                                                                                                                                >  1:118218/1‑251 (MQ=255)                                                                   gggtccGGTACTGGGCTTCCTTATCGCCGTCTGCATTATTGGCCCGTGGGGGCTGCGACGGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCt                                                                                                                                                                                 <  2:452828/246‑1 (MQ=255)                                                                   gggATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTgc                                                                                                                                                                                    <  1:202943/248‑1 (MQ=255)                                                                    ggATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTAGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTTT‑Gcctggttctttggctggggtg                                                                                                                                                                                >  2:150622/1‑231 (MQ=255)                                                                        cGGTACTTGGCTACCTGATCGCCAGCGGCATTATTGGCCCGTGGGGAATGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCGGAGATTGGGGGGGTGCTGAAGCTGTTTATTATCGGCCTCGAACACGACACCCAAAGGCTGAGGAAGCTGCGAGCGGCAGGGTTCGGGGGGGTGGCATTGCAGAGGGGGATTGGTGGAGGACGGCTGGGGCTGTGTT‑GCAGGTTTCTTGGGCttggctggg                                                                                                                                                                              >  2:485520/1‑241 (MQ=255)                                                                                ggCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCgg                                                                                                                                                                     <  1:21750/250‑1 (MQ=255)                                                                                 gCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCctgct                                                                                                                                                                                                                  <  2:422806/205‑1 (MQ=255)                                                                                 gCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCctgct                                                                                                                                                                                                                  >  1:422806/1‑205 (MQ=255)                                                                                      cTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGACTCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGTTGGTGGTTTGCGGCGGCCTGCTGGGGCTGGTAT‑GCCTGTTTCTTGGGCTGCGCTGGCGGGGCGCGGGATTggt                                                                                                                                                              >  2:288574/1‑249 (MQ=255)                                                                                        gATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCTCTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCg                                                                                                                                                            <  1:275348/251‑1 (MQ=255)                                                                                            gCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGCATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGGGATTTGCGGCGGACTGCTGGGGCTGTTTT‑GCATGTTACTTGGGCTGCGCTGGCCGGTCGCGGGATTGCTTGGCAt                                                                                                                                                        >  2:481639/1‑251 (MQ=255)                                                                                             ccGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGGTCT‑GCATGTTACTTTGGCTGCGGTGGCAGGTCGCGGGATTGATCGGCATg                                                                                                                                                       >  1:105671/1‑251 (MQ=255)                                                                                                  tGCATTATTGGCACGTGGGGGCTGCGACTGGTGACCGATGCCGACTCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCCTTGCAGATGGTTATTTGCGGCGGCCTGCTGGGGCTGTTCTTGC‑TGTTACTTGGGCTGCGCTTGCCGGGCCCGGAATTgtacggatgtaagct                                                                                                                                                  >  2:90548/1‑237 (MQ=255)                                                                                                     attattGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGgc                                                                                                                                               <  2:178519/251‑1 (MQ=255)                                                                                                                gTGGGGGCGGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTa                                                                                                                                    <  2:225800/251‑1 (MQ=255)                                                                                                                 tGGGGGCTGCGACTGGTGACCGATGCCGAATATATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGGGGCGCATTGCAGATGGGGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAGTTGATCGGCATGACGCTGGCGCTCTCCTCTAc                                                                                                                                   >  1:175816/1‑251 (MQ=255)                                                                                                                  gggggCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGTGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGTCGGCTTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGTGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTAc                                                                                                                                   <  1:407354/250‑1 (MQ=255)                                                                                                                         gCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTg                                                                                                                           <  1:420942/251‑1 (MQ=255)                                                                                                                         gCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGAGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGTGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGAt                                                                                                                             <  1:69956/249‑1 (MQ=255)                                                                                                                           gACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAAGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGcc                                                                                                                         >  1:409175/1‑251 (MQ=255)                                                                                                                             cTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGCGATTGGGGAGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCG‑GCATGTTACTTGGGCTGCGCTGGCAGGTTGCGGAATTTGTCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCAt                                                                                                                       >  1:285689/1‑251 (MQ=255)                                                                                                                                gTGACCGATGCCGAATCTATTCTGCGCTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAAGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCa                                                                                                                    <  2:409175/251‑1 (MQ=255)                                                                                                                                       attccGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTAGCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTGCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGatg                                                                                                             <  2:131804/248‑1 (MQ=255)                                                                                                                                         gCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCGGGCGCTCTCCTCTACGGCGATTGCCAGGCAGGCGatgaa                                                                                                           <  1:103220/251‑1 (MQ=255)                                                                                                                                          ccGAATCTATTCTGCACTTGGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTAGCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGatgaat                                                                                                          <  2:134270/251‑1 (MQ=255)                                                                                                                                                  tttCTGCTCTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTTCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGGGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTATGGCGATTGCCATGCAGGCGATGAATGAACGCa                                                                                                   <  1:306211/249‑1 (MQ=255)                                                                                                                                                       gCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATc                                                                                                <  1:182192/248‑1 (MQ=255)                                                                                                                                                            ttGCCGAGATTGGGGTGGGGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATgg                                                                                          <  1:464164/249‑1 (MQ=255)                                                                                                                                                             tGCCGAGATTGGGGGGGTGAGGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTGCGGCGGTGGCGCATTGCAGATGGTGATGTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCTGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATTAATGACCGCAAGCTGATGGTg                                                                                        <  2:66269/250‑1 (MQ=255)                                                                                                                                                              gCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCCCAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGAc                                                                                      <  1:463941/251‑1 (MQ=255)                                                                                                                                                              gCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGAc                                                                                      <  1:327891/251‑1 (MQ=255)                                                                                                                                                               ccGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATTTTACTTGGGCTGCGCTGGCAGGTCGCGGGATTGATCGGCCTGACGCTGGCGCTCTCCTCTTCGGGGGTTTCCATGCCGGGGatgaatagacaccatcattaggggacg                                                                                     >  2:378389/1‑230 (MQ=255)                                                                                                                                                                    aTTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGACGGCGATGAATGAACGCCATCTGATGGTGACGCAAAt                                                                                >  1:329771/1‑251 (MQ=255)                                                                                                                                                                          gtggtgCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGGCGGGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGCTGAATGAACGCAATCTGATGGTGACGCAAATggggcg                                                                          >  2:152753/1‑248 (MQ=255)                                                                                                                                                                                cTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGc                                                                    >  1:300703/1‑251 (MQ=255)                                                                                                                                                                                  gATGCTGTTTCTTATCGGCCGCGAACTCGATCCACAAAGGCTGTGGAAGCGGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGAGTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGcc                                                                   <  2:358800/250‑1 (MQ=255)                                                                                                                                                                                  gATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGGGATTTGCGGAGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGGATTGGTCGGACTGGCGCTGGCGCTCTCCTCTACGGCGGTTGCCCTGCCGGCGGTGGATGGACGCAATCTGGTGGTGgcgcgagtggggcgcggggcct                                                                  >  2:115607/1‑233 (MQ=255)                                                                                                                                                                                               atCGGCCTCGAACTCTATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTTGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGtgctgc                                                      <  1:366748/250‑1 (MQ=255)                                                                                                                                                                                                  ggCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGtt                                                  <  1:193164/251‑1 (MQ=255)                                                                                                                                                                                                     cTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCATTGCCTTTGCGGTGCTGCTGTTccc                                               >  1:94783/1‑250 (MQ=255)                                                                                                                                                                                                        gAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAgg                                             >  1:114929/1‑250 (MQ=255)                                                                                                                                                                                                         aaCTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGa                                            >  1:19438/1‑250 (MQ=255)                                                                                                                                                                                                                 ccACAAAGGCTGTGGAAGCTGCGGGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCgcggc                                   <  1:278590/251‑1 (MQ=255)                                                                                                                                                                                                                 ccACAAAGGCTGTGGAAGCTGCGGGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTGACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCgcgg                                    <  2:169180/250‑1 (MQ=255)                                                                                                                                                                                                                    caAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGAt                                <  1:356392/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaGGCTGGGGAAGCTGCGGGCGGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATc                               <  2:118218/251‑1 (MQ=255)                                                                                                                                                                                                                            gtgGAAGGTGCGTGCGGCAGTGTTCGGCGGTGGCGGATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCGGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCtgg                        <  1:360556/251‑1 (MQ=255)                                                                                                                                                                                                                                         gcgGCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTcc           <  1:354282/251‑1 (MQ=255)                                                                                                                                                                                                                                            gCAGTGTTCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGAAATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCt        >  1:264745/1‑251 (MQ=255)                                                                                                                                                                                                                                                  ttCGGCGGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGGTGCGCTGGCAGGGCGCGGGATTGATCGGCATGGCGCTGGCGCTCTCCTCTTCGGCGATTGCCATGCGGGCGGTGAATGAACGCAATATGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGAGATGATTCCGCTGCTGGc  >  1:81714/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAGATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGGAAGCTGCGTGCGGCAGTGTTCGGCTGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTGCTGGGGCTGTTCT‑GCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATGACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATGGTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATCCCGCTGGTGGCGATGATTCCGCTACTGGC  >  NC_000913/47786‑48277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑