Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,976	A→G	L390L (CTA→CTG)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,976	0	A	G	97.8%	147.4 / NA	46	L390L (CTA→CTG)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (17/28);  minor base C (0/1);  total (17/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAAC  >  NC_000913/46732‑47220                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATc                                                                                                                                                                                                                                                <  1:175356/251‑1 (MQ=255)   ccATTGTTTATTTGCAGCTTTGCCATGATGACGCTGGCGCTGGCTGTTTGGGGGCTGATCCCGGATTTGGGGATTGGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCTTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTTGCCTAATGAACCCTCCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGacct                                                                                                                                                                                                                                               <  2:118528/250‑4 (MQ=255)                                 aCGCTGGCGCTGGCGGTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGAt                                                                                                                                                                                                                <  1:122179/251‑1 (MQ=255)                                        ccgtggcgGTTTTGGGGCTGATCCCGGATATGGGGATCTGGGTGGT‑GTGGTTGGCCTTTGCGGGGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATg                                                                                                                                                                                                         <  2:405396/248‑1 (MQ=255)                                               ggTTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCgg                                                                                                                                                                                                  <  1:361695/251‑1 (MQ=255)                                                     ggggCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTCATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGACAATGTGGGCGGGTctct                                                                                                                                                                                            >  1:39039/1‑251 (MQ=255)                                                     ggggCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTAtc                                                                                                                                                                                             <  1:168155/250‑1 (MQ=255)                                                       ggCTGATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCg                                                                                                                                                                                          >  1:13890/1‑251 (MQ=255)                                                             tCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTGTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGtt                                                                                                                                                                                     <  2:13890/250‑1 (MQ=255)                                                              cccGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGttt                                                                                                                                                                                    <  1:247246/250‑1 (MQ=255)                                                                   atatGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCttg                                                                                                                                                                              >  1:476935/1‑251 (MQ=255)                                                                          gATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGGTTGGCTTGTTGAttt                                                                                                                                                                       >  1:354701/1‑251 (MQ=255)                                                                                gctggTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAg                                                                                                                                                                 <  1:377624/251‑1 (MQ=255)                                                                                   ggTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTa                                                                                                                                                                                                                                  >  1:265268/1‑183 (MQ=255)                                                                                   ggTAGTG‑ATGGCCTTGGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTa                                                                                                                                                                                                                                  <  2:265268/183‑1 (MQ=255)                                                                                           atggctgtgtgggTGTAGGCCTTTGTCTGTGGGGTGCCGGGTAATTTTCATTGGCTCTTTCTTAAGTAACTTTTTCCGTCAGTTAGCCGCGCCTTTGCCTTGGGCGTGATTATGTCCTTAAGCTGTATTGGCTCCATTGTTTCGACCTGGGCACTGCCGATCTGTCTCAATAAGTACGGTTTCAGTAACACGATGCTAATGTTGGCGTGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTcccc                                                                                                                                                        <  2:210821/239‑1 (MQ=255)                                                                                           atggctgtggcgggttATGCCTTTTTCTTTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATTAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTTGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGcccc                                                                                                                                                       <  2:104022/236‑1 (MQ=255)                                                                                             ggccttgagggTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCATGGGCTCTATCCTAAGGAATTCTTCCCGACAGGTTTCCGCGCCTCGGCCGTGGGCGTGATTAGGGCCTTAAGCCGTATTGGCACCATGGTTTCGTCCTGGGCTCTGCCGATCTTTATCAAGAATTACGGTATCAGTAACACGATGCTAATGGGGGAGGGTATCTCGCTGTGTGGCGTGTTGATGTCCGTAGCGTTTGCCCCgg                                                                                                                                                     <  2:298084/242‑1 (MQ=255)                                                                                                 ttttgtgtgattGCCTTTTCTTCTGGCGGGCCGGGTAAATTGCAGTGGCCCTATCCTAATGAATTCCTCCCGACAGATATCCGCGCCTCTGCCGAGGGCTTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGgaga                                                                                                                                                  <  2:161101/239‑1 (MQ=255)                                                                                                      ggTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAg                                                                                                                                            <  1:382829/251‑1 (MQ=255)                                                                                                                       ctGGCGGGCCGGGTAATTTGCCGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATTTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGAATCTCGCGGTTTGGCTTGTTGATTTCCGTAACGTTTGCCCCGGAGACTCGGGGGATGTCACTGGCGCa                                                                                                                            >  2:87109/1‑250 (MQ=255)                                                                                                                            gtgCCGGGTAATTTTCAGGGGTTTTATCCTAATGAAGTCTCCCCGTCAGTGATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATGGTTTCGACCTGGGCACTGCCGATTTTTATTAATAATTACGGTATCAGTAACACGATGCTAAGGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGTCTCGAGGGATGTCTCTGGCGCAAACCAg                                                                                                                      <  2:439510/249‑1 (MQ=255)                                                                                                                                   gTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATg                                                                                                               >  2:215304/1‑251 (MQ=255)                                                                                                                                     aaTTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGAc                                                                                                             >  1:141134/1‑251 (MQ=255)                                                                                                                                     aaTTTGCAGGGGCTCTATCCAAATGAATCCTCCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCGGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGAc                                                                                                             <  2:287513/251‑1 (MQ=255)                                                                                                                                         tGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGCTATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCCCTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGTGCGGGTATCTCGCATTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGGGACACGGGGGATGTCACTGGCGCAAACCAGCAATATGACGaac                                                                                                         >  2:305306/1‑249 (MQ=255)                                                                                                                                                gctttatcataattaacgcgtCCCGACATATATCCGCGCCTCTGCCGTGGGCGGGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGc                                                                                                  <  2:486231/231‑1 (MQ=255)                                                                                                                                                   ctATCCTAATGAACCCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTTGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCa                                                                                                 <  1:320574/249‑1 (MQ=255)                                                                                                                                                          aaTGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATggg                                                                                        <  1:143935/251‑1 (MQ=255)                                                                                                                                                           aTGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGGATgggg                                                                                       >  1:359429/1‑251 (MQ=255)                                                                                                                                                            tGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCACTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATggggg                                                                                      >  1:216685/1‑250 (MQ=255)                                                                                                                                                                  ctTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGCCTCGAGGGATGTAACTGGCGCAAACCAGCACTATGACGATCCGCGGGCAGAGAATGGGGTAAATTg                                                                                >  1:392886/1‑251 (MQ=255)                                                                                                                                                                               atCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTCCCGATCTTTATCAATAAGTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGTCGCAAACCAGCAATATGATGATCCGCGGGCAGATAATGGGGTAAATTGTTCAGATTtctc                                                                    <  1:394494/250‑1 (MQ=255)                                                                                                                                                                                       ctctGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGcccc                                                                                                                                                       <  1:326498/159‑1 (MQ=255)                                                                                                                                                                                       ctctGCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGcccc                                                                                                                                                       >  2:326498/1‑159 (MQ=255)                                                                                                                                                                                           gCCGTGGGCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATc                                                       <  1:421461/251‑1 (MQ=255)                                                                                                                                                                                                gggCGTGATTATGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAatat                                                  <  1:33825/251‑1 (MQ=255)                                                                                                                                                                                                          aTGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAgg                                                                                                                                           >  1:422554/1‑152 (MQ=255)                                                                                                                                                                                                          aTGTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAgg                                                                                                                                           <  2:422554/152‑1 (MQ=255)                                                                                                                                                                                                            gTCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTAGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATaa                                      <  1:393187/251‑1 (MQ=255)                                                                                                                                                                                                             tCCTTAAGCCGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACtata                                       >  1:101789/1‑249 (MQ=255)                                                                                                                                                                                                                     ccGTATTGGCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGCGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTg                              >  1:231710/2‑250 (MQ=255)                                                                                                                                                                                                                            ggCACCATTGTTTCGACCTGGGCACTGCCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAatatat                       <  1:167408/250‑1 (MQ=255)                                                                                                                                                                                                                             gCACCCTTGTTTCTACCTGGGCAAGGCTGATCTTTATCAAGAATTACGGTCACAGTAACACGATGCTAATTGGTGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCTGAGACTCGAGGGTGGTCACTGGCTCAAACCAGCAATATGACGATTCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTGTTCTGAATCAAGATTATTGACTATAAGCCGCGTGAATATATGa                     <  2:279822/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGACCTGGGCACTGCCGGTCTTTATCAATAATTACGGTATCAGAAACACGCTGCTAATGGGGGCGGGTCTATCGCTGTTTGGATTGTTGATTTCCGTAGCGTTTGCCCCGGAGCCTCGAGGGGTGAAACTGGCGCAAACCCGCAATATGACGATCCGCGGGCAGGGAAAGGGGTAAATTGTTCCGATTTCTCTCTTTTCTGCATCAATATTATTGACTATAAGCCGCGTGCATCTATGACTACACTTtgtg         >  2:24634/1‑251 (MQ=255)                                                                                                                                                                                                                                                ggCACTGCCGATCTTTAGCAAGAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAAACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAAGTGTTCAGATTTCTCTCTTTTCTGAATCAATAGTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAAc  <  1:24634/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGTTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTG‑ATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGACAGATATCCGCGCCTCTGCCGTGGGCGTGATTATGTCCTTAAGTCGTATTGGCACCATTGTTTCGACCTGGGCACTACCGATCTTTATCAATAATTACGGTATCAGTAACACGATGCTAATGGGGGCGGGTATCTCGCTGTTTGGCTTGTTGATTTCCGTAGCGTTTGCCCCGGAGACTCGAGGGATGTCACTGGCGCAGACCAGCAATATGACGATCCGCGGGCAGAGAATGGGGTAAATTGTTCAGATTTCTCTCTTTTCTGAATCAATATTATTGACTATAAGCCGCGTGAATATATGACTACACTTTGTGGGAAAAC  >  NC_000913/46732‑47220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑