Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,448	C→A	I214I (ATC→ATA)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,448	0	C	A	100.0%	157.9 / NA	49	I214I (ATC→ATA)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/23);  total (26/23)

CCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTC  >  NC_000913/46210‑46679                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        ccAGTCCCCGTCAGCGGGCGTTTTCCATCAGCTGTAGCGCCGCCATGTGGGATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGttt                                                                                                                                                                                                                                <  2:310118/248‑1 (MQ=255) ccAGGACCCGTCAGCGGGCGTTTTCCATCAGATTTATCGACGCCATGTGGTATGTCGGCGCGACATGCGCCGATCTTGTCGGAGACTGGCTTTATGATGTGGAAGGCGGCTGGTGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGACTTTGATTGGTCGATTCGAACGGCCTGAATCTCCCAGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGAGAAGAGATGATGATAAAACTGTTTGGc                                                                                                                                                                                                                             <  1:1836/251‑1 (MQ=255)   aGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCg                                                                                                                                                                                                                            >  1:234556/1‑250 (MQ=255)       cccGTCAGCGGACGATTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTTTTTGCTTGGTCGATTCGCACTGCCTGAATCTCCCCGCTGGTTATTACGCAAGGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAACCTGTTTGGCGTACCg                                                                                                                                                                                                                       >  2:458954/1‑251 (MQ=255)             ggCGGGCTTTTTCCATCAGTTTTATCGCCGCCATGTGGGATGGCGGCGGGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGATGAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCt                                                                                                                                                                                                                 <  2:136581/250‑1 (MQ=255)                                           ccaTGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGt                                                                                                                                                                                   <  2:214971/249‑1 (MQ=255)                                              tgtgGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAACGGGCGAGTAAAAGAGTGCGAAGCGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGACAACCCGtttt                                                                                                                                                                                >  2:181680/1‑251 (MQ=255)                                                    aTGTCGGCGCGACCTGCGCCGATCTGGTCGGATACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGATGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGAt                                                                                                                                                                          >  1:381699/1‑251 (MQ=255)                                                    aTGTCGGCGCGACCCGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAAAAGCCGCAGCAAACCCGTTTTCGCGAt                                                                                                                                                                          >  2:341249/1‑251 (MQ=255)                                                        cgtcgcgACCTGCGCCGAGCTGGTAGTCTACGGGCTTTATGATGTGGAAGGCGGCTTGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGCTTGTTGATTTTGAGTGTTCGATGCGAACGGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGTGAACCGGGGGCTTTCGATGAAGATCAGCCGCAGCaaatactttttcgttagctg                                                                                                                                                                       <  2:457574/247‑18 (MQ=255)                                                         ggCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTAGCGATCTGtt                                                                                                                                                                     >  1:294407/1‑251 (MQ=255)                                                             cgACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATTTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGTCGCAGCAAACCCGGTTTCGCGATCTGTTTAAt                                                                                                                                                                 <  1:128233/251‑1 (MQ=255)                                                                                     aCTGGCTTTAGGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCt                                                                                                                                          <  1:84212/250‑1 (MQ=255)                                                                                        ggCTTTATGATGTGGAAGGCGGCTGGCGCTGGAGGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCtgtt                                                                                                                                       <  2:191055/250‑1 (MQ=255)                                                                                                   gtgGAGGGGGGCTGGTGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTGTTTGATTGGGCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACGGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCa                                                                                                                            <  2:435974/250‑1 (MQ=255)                                                                                                     ggAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCa                                                                                                                            >  1:435974/1‑248 (MQ=255)                                                                                                      gAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTg                                                                                                                        >  2:500722/1‑251 (MQ=255)                                                                                                       aaGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTgg                                                                                                                       <  1:431738/251‑1 (MQ=255)                                                                                                                ggCGCTGGAGGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAg                                                                                                              <  1:500722/251‑1 (MQ=255)                                                                                                                     gggttGCTGGGTAGCTCGGCGATCCCCTGTTTGGTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGAt                                                                                                         <  2:9606/247‑1 (MQ=255)                                                                                                                             gggTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATg                                                                                                  <  1:184305/250‑1 (MQ=255)                                                                                                                               gTAGCGCGGAGATCCCCTGTTTGTTGATTTTGATTGGTCGATGCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTTTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTc                                                                                               <  1:259050/251‑1 (MQ=255)                                                                                                                                    gcggcgATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCAt                                                                                          >  2:348626/1‑251 (MQ=255)                                                                                                                                      ggcgATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCCCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCCATGTTCGCCAttt                                                                                        >  2:125485/1‑251 (MQ=255)                                                                                                                                         gATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGTACTGCCTGATTCTCCCCGCTGGTTATTCCGCAAAGGGCGAGTAAAAGAGTGCGAAGGGCTGCTGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAGGAGCGGCCGCAGCAAACCCGTTTTCGCGATCTTTTTACTCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCGGGAGTTCCCACTGTTCCCCTTTTacc                                                                                     >  2:175356/1‑250 (MQ=255)                                                                                                                                            ccgatttttgttGATTGTGATTGGTCGATTAGAACCTCCTGAATCGCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTGGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGGGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCt                                                                                  <  2:459810/245‑1 (MQ=255)                                                                                                                                             cccTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTACAAGAGTGCGACGAGCTGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGCTGAAGAGCAGCCGCAACAAACCCGTTTTCGCGATCTGTTTACTCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGCCCTGACAGGAGAGCCCACTGTTCGGCATTTACCCCtt                                                                                 >  1:457427/1‑251 (MQ=255)                                                                                                                                                 gtttgttGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTg                                                                               <  1:341249/249‑1 (MQ=255)                                                                                                                                                   ttgttgGTTTTGATTGGTCGATTCGCACTACCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCCAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGGTTGTTGCCGCCATCTGGACCTGCCAGGTAATACCAATGTTCGCCTTTTACACCtcgatcgg                                                                           >  1:459810/1‑244 (MQ=255)                                                                                                                                                      ttgattttgattGGTAGATTCGAACTGCCAGAATCTCTCCGCTAGTTATTACGAAAAGGGCGAGTAACAGTGAGCGAAGAGATGATGCTAAAACTGTTTGGCGAACCGGTGGCTTTCGAGGAAGAGCAGCAGCAGCAAACCCGTTTTAGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCAATATGGACCTGCCTGTTGCTACCAATGTTCGACTTTTACACCTTTAGCCCGc                                                                         >  1:360672/1‑250 (MQ=255)                                                                                                                                                       tgattttgattGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGcc                                                                                            >  1:458427/1‑230 (MQ=255)                                                                                                                                                       tgattttgattGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGcc                                                                                            <  2:458427/230‑1 (MQ=255)                                                                                                                                                                    tCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCGGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGAttgt                                                          >  1:161101/1‑251 (MQ=255)                                                                                                                                                                           gAACTGACTGAATCTCCCCGCTGGTTAATACGCAAAGGCCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAAACGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGAGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTATGTTGGGAtt                                                   >  1:200021/1‑251 (MQ=255)                                                                                                                                                                             aCTGCCTGAATCTCCCCGCTGGGTATTACGCAAAGGGCGAGTGAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTgg                                                 >  2:75861/1‑251 (MQ=255)                                                                                                                                                                                           cccGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAAc                                   <  1:134801/251‑1 (MQ=255)                                                                                                                                                                                             cGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAAcgc                                 <  1:348626/251‑1 (MQ=255)                                                                                                                                                                                                 ggTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGc                              <  1:425862/250‑1 (MQ=255)                                                                                                                                                                                                  gTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGCTGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGGGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAACTCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGcgc                            >  2:421461/1‑249 (MQ=255)                                                                                                                                                                                                     attaCGCAAAGAGCGCGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTATGGCGAACCGGTAGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATAGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGACAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGATTGTTGGGATTGGGTGTTGGATAAAACGCGGcgctgg                         >  2:284631/1‑246 (MQ=255)                                                                                                                                                                                                          gCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGACCCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGaac                    <  2:482989/251‑2 (MQ=255)                                                                                                                                                                                                           cAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGaacg                   >  1:205356/1‑249 (MQ=255)                                                                                                                                                                                                              aGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACgtgg                >  1:228654/1‑251 (MQ=255)                                                                                                                                                                                                                      tAAAAAAGAGCGAAGAGATGATGATATAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCAGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACATTTGGCCAGCAACTCGTTGGATTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGTTTAGc        >  2:320574/1‑251 (MQ=255)                                                                                                                                                                                                                           gagTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGtt   <  1:311097/251‑1 (MQ=255)                                                                                                                                                                                                                           gagTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGAGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGtt   >  1:298084/1‑251 (MQ=255)                                                                                                                                                                                                                            agTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAACTCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTc  >  2:388254/1‑251 (MQ=255)                                                                                                                                                                                                                                 gAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCTTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAgg                                                                                                             <  2:449054/139‑1 (MQ=255)                                                                                                                                                                                                                                 gAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAgg                                                                                                             >  1:449054/1‑139 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        CCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTC  >  NC_000913/46210‑46679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑