Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,277	T→C	C157C (TGT→TGC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,277	0	T	C	100.0%	158.6 / NA	53	C157C (TGT→TGC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/25);  total (28/25)

ATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAAT  >  NC_000913/46030‑46520                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCTTGATAGCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGcc                                                                                                                                                                                                                                                  <  1:6024/251‑1 (MQ=255) atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGcc                                                                                                                                                                                                                                                  >  1:250822/1‑251 (MQ=255)               gTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCt                                                                                                                                                                                                                                     >  1:191055/1‑250 (MQ=255)                  ggACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGGGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATACGCGTTATCGCCGCCATGTGGTATGTCGACGCGACCTGCGCCGATCTTGTCGGCTACTg                                                                                                                                                                                                                                 >  2:128233/1‑251 (MQ=255)                   gACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCCGATTATCCCATCGCCACCTCAATGATCACCGGGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGCCGGCTACTgg                                                                                                                                                                                                                                >  2:184305/1‑251 (MQ=255)                   gACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTgg                                                                                                                                                                                                                                >  1:170466/1‑251 (MQ=255)                            aaaTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCCTCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCCGCTTTATCGCCGCCATGTGGTATGTCGGCGCGCCCTGCGCCGATCTGGTCGGCTACTGGCttttttgt                                                                                                                                                                                                                       >  2:403807/1‑246 (MQ=255)                             aaTGTTCCTCATTGATATCATCTCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCAACCCCCGTCGAACTGGTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTTCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGTGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTatgatg                                                                                                                                                                                                                      <  1:475286/251‑1 (MQ=255)                                 ttCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTTGTCGGCTCCTGGCTTTATGGTGTggg                                                                                                                                                                                                                  >  2:102744/1‑250 (MQ=255)                                  tCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGa                                                                                                                                                                                                                  >  1:9606/1‑250 (MQ=255)                                          gATATCATCGCCATCGGCGTGAGATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCt                                                                                                                                                                                                          <  2:256221/250‑1 (MQ=255)                                                  cGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTCCTTATCGGCATTGTCATCGGTGCAGCTTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTATCCATCAGCTTTATCGCCGCCATGTGGTATGGCGGCGCGCCCTGCGCCGATCTTGTCGGCTCCTGGCTTTATGGTGTGGGAGGCGGCTGGCGCttgg                                                                                                                                                                                                 >  2:148196/1‑248 (MQ=255)                                                     cATCGGCGTGATATCGGTGGCGAAGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCTCCGCCATGTGGTATGTCGGCGCGGCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGACGGCGGCTGGCGCTGGATGc                                                                                                                                                                                              >  2:431738/1‑251 (MQ=255)                                                            gTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAg                                                                                                                                                                                       <  2:267522/251‑1 (MQ=255)                                                               atatCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAgcgc                                                                                                                                                                                    <  1:102744/251‑1 (MQ=255)                                                                tatCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGAt                                                                                                                                                                                                >  1:42076/1‑238 (MQ=255)                                                                tatCGGGGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGAt                                                                                                                                                                                                <  2:42076/238‑1 (MQ=255)                                                                 atCGGTGGGGACGATGTTTGGTTCATCCCCCGGCGAACGGTTGGTGAGGCGGGGACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTGTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCgcgg                                                                                                                                                                                  <  2:70756/251‑1 (MQ=255)                                                                    ggTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCgcggcgg                                                                                                                                                                               >  1:482989/1‑250 (MQ=255)                                                                                       ccATCCCCCGGTGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATGATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCTTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCAGGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTAGGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGAGCCCCTGTTTGttgatttt                                                                                                                                                            <  2:218949/250‑1 (MQ=255)                                                                                             cccGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTGCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTGGATTTTGATTgg                                                                                                                                                      <  2:55760/251‑1 (MQ=255)                                                                                                   gAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGGTTTTGATTGGTCGAtt                                                                                                                                                >  1:361705/1‑251 (MQ=255)                                                                                                      cTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGCTGTGGAAGGCGGCTGGCGCTGGATGATGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGTTTGGTCGATTCg                                                                                                                                              >  2:4726/1‑250 (MQ=255)                                                                                                              gATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTg                                                                                                                                     <  1:403807/251‑1 (MQ=255)                                                                                                                     gTACTTAGCAGCATTGGAATCGGAGCAGATTATCCCATCGCCACCACAATAATAACCGAGTTCTCAAGAACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTTCTCAGGTCTGGTAGCCTACTGGCTTTATGCTGTGGCAGGCGGCTGGCGCGGGATGCTGGGAGGCGCGGCTGTCCCCTATTTGTCGTTTTTGCTTGGTCAATTCAACCTGCATGaaccacc                                                                                                                              >  2:18873/1‑246 (MQ=255)                                                                                                                        cTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTTCCTGAATCTCCCCg                                                                                                                           >  1:260088/1‑251 (MQ=255)                                                                                                                           aTCGGCATGGTCACCGGTGCAGATTATTCCAGCGCCACCTCAATTACCACCGTGCTCTCCAGTACCCGTCAGCGGGCGTTTACCATCAGCTTTATCGCCGCCAGGTTGTATGTCGGCGCGTCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCACCTGTTTGTTGATTTTGATTGGGCGATGCGAACTGCCTGAAGCTCCCCGCTg                                                                                                                        <  2:189842/251‑1 (MQ=255)                                                                                                                              ggCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGtt                                                                                                                     >  1:120805/1‑251 (MQ=255)                                                                                                                               gCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCATGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGtt                                                                                                                     >  1:197740/1‑250 (MQ=255)                                                                                                                                     tcctctgggCAGAGTATCCCTTCGCCACCTCAATGATCACCGATTTCTCCAGTCCCCGTCAGCGGGCGTTTTCCATCATCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGTCTACTGGCTTTATGATGTTGAAGGCGGCTGGCCCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTTTTGATTTTGATTGGTCGATTCGATCGGCCTCAATCTCCCCGCTGGCTATTACg                                                                                                               <  2:68794/242‑1 (MQ=255)                                                                                                                                             gCAGATTATCCCATCGCCGCCTCAATGATCACCGAGTTCCCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGACGCGTCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTTGCGCGGCGCTCCCCTGTTTGTTGAGTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGc                                                                                                       <  1:240044/250‑1 (MQ=255)                                                                                                                                                gATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGt                                                                                                   <  2:120805/251‑1 (MQ=255)                                                                                                                                                aaTTATCCCATCGCCACTTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCTATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGt                                                                                                   <  2:477456/250‑1 (MQ=255)                                                                                                                                                     tCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTaaa                                                                                                >  1:421322/1‑249 (MQ=255)                                                                                                                                                      cccAACGCTACCTCAATGATCACCGAGGTCGCCAGTTCCCGGCAGCGGGCGTTTGCCATCAGCTTTATCGCCGCCATTTGGTATGTCGGCGCGACCTGCGCCGATCTGGGCTGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGACGAATCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAaga                                                                                             <  2:407784/251‑1 (MQ=255)                                                                                                                                                           cGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTg                                                                                          <  2:250822/249‑1 (MQ=255)                                                                                                                                                                 cTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGCTGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTTTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGGGTGCGAag                                                                                     >  2:425862/1‑248 (MQ=255)                                                                                                                                                                                     ccAGTCCCCGTCAGCGGGCGTTTTCCATCAGCTGTAGCGCCGCCATGTGGGATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGttt                                                                 <  2:310118/248‑1 (MQ=255)                                                                                                                                                                                     ccAGGACCCGTCAGCGGGCGTTTTCCATCAGATTTATCGACGCCATGTGGTATGTCGGCGCGACATGCGCCGATCTTGTCGGAGACTGGCTTTATGATGTGGAAGGCGGCTGGTGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGACTTTGATTGGTCGATTCGAACGGCCTGAATCTCCCAGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGAGAAGAGATGATGATAAAACTGTTTGGc                                                              <  1:1836/251‑1 (MQ=255)                                                                                                                                                                                       aGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCg                                                             >  1:234556/1‑250 (MQ=255)                                                                                                                                                                                           cccGTCAGCGGACGATTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTTTTTGCTTGGTCGATTCGCACTGCCTGAATCTCCCCGCTGGTTATTACGCAAGGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAACCTGTTTGGCGTACCg                                                        >  2:458954/1‑251 (MQ=255)                                                                                                                                                                                                 ggCGGGCTTTTTCCATCAGTTTTATCGCCGCCATGTGGGATGGCGGCGGGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGATGAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCt                                                  <  2:136581/250‑1 (MQ=255)                                                                                                                                                                                                                        atcgccgccATGTGGTATGTCGGCGCGACCTGCGCCGCTCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGGGTGCGAAGAgat                                                                                 >  2:478019/4‑197 (MQ=255)                                                                                                                                                                                                                        atcgccgccATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAgat                                                                                 <  1:478019/194‑1 (MQ=255)                                                                                                                                                                                                                               ccaTGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGt                    <  2:214971/249‑1 (MQ=255)                                                                                                                                                                                                                                  tgtgGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAACGGGCGAGTAAAAGAGTGCGAAGCGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGACAACCCGtttt                 >  2:181680/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGTCGGCGCGACCTGCGCCGATCTGGTCGGATACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGATGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGAt           >  1:381699/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGTCGGCGCGACCCGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAAAAGCCGCAGCAAACCCGTTTTCGCGAt           >  2:341249/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgtcgcgACCTGCGCCGAGCTGGTAGTCTACGGGCTTTATGATGTGGAAGGCGGCTTGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGCTTGTTGATTTTGAGTGTTCGATGCGAACGGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGTGAACCGGGGGCTTTCGATGAAGATCAGCCGCAGCaaatactttttcgttagctg        <  2:457574/247‑18 (MQ=255)                                                                                                                                                                                                                                             ggCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTAGCGATCTGtt      >  1:294407/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cgACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATTTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGTCGCAGCAAACCCGGTTTCGCGATCTGTTTAAt  <  1:128233/251‑1 (MQ=255)                                                                                                                                                                                                                                                  gACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAg                                                                                                          >  1:188184/1‑146 (MQ=255)                                                                                                                                                                                                                                                  gACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAg                                                                                                          <  2:188184/146‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAAT  >  NC_000913/46030‑46520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑