Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,247	T→C	I147I (ATT→ATC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,247	0	T	C	100.0%	144.2 / NA	47	I147I (ATT→ATC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/25);  total (22/25)

CGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCT  >  NC_000913/46007‑46472                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  cGTTGGCACATCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCAtgt                                                                                                                                                                                                                         <  1:204287/251‑1 (MQ=255)         caTCGCTGTTTGGCTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCTTCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTc                                                                                                                                                                                                                 <  2:29988/251‑1 (MQ=255)                   tggctataTTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGAcc                                                                                                                                                                                                        <  1:286203/246‑1 (MQ=255)                       tataGTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGTGATCATCGCCATCGGCGTGATATCGGCCGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCGCCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCtgcg                                                                                                                                                                                                    <  1:452191/250‑3 (MQ=255)                        atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCTTGATAGCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGcc                                                                                                                                                                                                  <  1:6024/251‑1 (MQ=255)                        atatTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGcc                                                                                                                                                                                                  >  1:250822/1‑251 (MQ=255)                                      gTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCt                                                                                                                                                                                     >  1:191055/1‑250 (MQ=255)                                         ggACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGGGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATACGCGTTATCGCCGCCATGTGGTATGTCGACGCGACCTGCGCCGATCTTGTCGGCTACTg                                                                                                                                                                                 >  2:128233/1‑251 (MQ=255)                                          gACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCCGATTATCCCATCGCCACCTCAATGATCACCGGGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGCCGGCTACTgg                                                                                                                                                                                >  2:184305/1‑251 (MQ=255)                                          gACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTgg                                                                                                                                                                                >  1:170466/1‑251 (MQ=255)                                                   aaaTGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCCTCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCCGCTTTATCGCCGCCATGTGGTATGTCGGCGCGCCCTGCGCCGATCTGGTCGGCTACTGGCttttttgt                                                                                                                                                                       >  2:403807/1‑246 (MQ=255)                                                    aaTGTTCCTCATTGATATCATCTCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCAACCCCCGTCGAACTGGTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTTCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGTGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTatgatg                                                                                                                                                                      <  1:475286/251‑1 (MQ=255)                                                        ttCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTTGTCGGCTCCTGGCTTTATGGTGTggg                                                                                                                                                                  >  2:102744/1‑250 (MQ=255)                                                         tCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGa                                                                                                                                                                  >  1:9606/1‑250 (MQ=255)                                                                 gATATCATCGCCATCGGCGTGAGATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCt                                                                                                                                                          <  2:256221/250‑1 (MQ=255)                                                                         cGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTCCTTATCGGCATTGTCATCGGTGCAGCTTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTATCCATCAGCTTTATCGCCGCCATGTGGTATGGCGGCGCGCCCTGCGCCGATCTTGTCGGCTCCTGGCTTTATGGTGTGGGAGGCGGCTGGCGCttgg                                                                                                                                                 >  2:148196/1‑248 (MQ=255)                                                                            cATCGGCGTGATATCGGTGGCGAAGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCTCCGCCATGTGGTATGTCGGCGCGGCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGACGGCGGCTGGCGCTGGATGc                                                                                                                                              >  2:431738/1‑251 (MQ=255)                                                                                   gTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAg                                                                                                                                       <  2:267522/251‑1 (MQ=255)                                                                                      atatCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAgcgc                                                                                                                                    <  1:102744/251‑1 (MQ=255)                                                                                       tatCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGAt                                                                                                                                                >  1:42076/1‑238 (MQ=255)                                                                                       tatCGGGGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGAt                                                                                                                                                <  2:42076/238‑1 (MQ=255)                                                                                        atCGGTGGGGACGATGTTTGGTTCATCCCCCGGCGAACGGTTGGTGAGGCGGGGACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTGTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCgcgg                                                                                                                                  <  2:70756/251‑1 (MQ=255)                                                                                           ggTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCgcggcgg                                                                                                                               >  1:482989/1‑250 (MQ=255)                                                                                                              ccATCCCCCGGTGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATGATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCTTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCAGGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTAGGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGAGCCCCTGTTTGttgatttt                                                                                                            <  2:218949/250‑1 (MQ=255)                                                                                                                    cccGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTGCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTGGATTTTGATTgg                                                                                                      <  2:55760/251‑1 (MQ=255)                                                                                                                          gAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGGTTTTGATTGGTCGAtt                                                                                                >  1:361705/1‑251 (MQ=255)                                                                                                                             cTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGCTGTGGAAGGCGGCTGGCGCTGGATGATGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGTTTGGTCGATTCg                                                                                              >  2:4726/1‑250 (MQ=255)                                                                                                                                     gATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTg                                                                                     <  1:403807/251‑1 (MQ=255)                                                                                                                                            gTACTTAGCAGCATTGGAATCGGAGCAGATTATCCCATCGCCACCACAATAATAACCGAGTTCTCAAGAACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTTCTCAGGTCTGGTAGCCTACTGGCTTTATGCTGTGGCAGGCGGCTGGCGCGGGATGCTGGGAGGCGCGGCTGTCCCCTATTTGTCGTTTTTGCTTGGTCAATTCAACCTGCATGaaccacc                                                                              >  2:18873/1‑246 (MQ=255)                                                                                                                                               cTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTTCCTGAATCTCCCCg                                                                           >  1:260088/1‑251 (MQ=255)                                                                                                                                                  aTCGGCATGGTCACCGGTGCAGATTATTCCAGCGCCACCTCAATTACCACCGTGCTCTCCAGTACCCGTCAGCGGGCGTTTACCATCAGCTTTATCGCCGCCAGGTTGTATGTCGGCGCGTCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCACCTGTTTGTTGATTTTGATTGGGCGATGCGAACTGCCTGAAGCTCCCCGCTg                                                                        <  2:189842/251‑1 (MQ=255)                                                                                                                                                     ggCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATcgccgcc                                                                                                                                                                                                                             >  1:437270/1‑98 (MQ=255)                                                                                                                                                     ggCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGtt                                                                     >  1:120805/1‑251 (MQ=255)                                                                                                                                                     ggCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTGCTCCAGGACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGcctcc                                                                                                                                                                                                                             <  2:437270/99‑4 (MQ=255)                                                                                                                                                      gCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCATGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGtt                                                                     >  1:197740/1‑250 (MQ=255)                                                                                                                                                            tcctctgggCAGAGTATCCCTTCGCCACCTCAATGATCACCGATTTCTCCAGTCCCCGTCAGCGGGCGTTTTCCATCATCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGTCTACTGGCTTTATGATGTTGAAGGCGGCTGGCCCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTTTTGATTTTGATTGGTCGATTCGATCGGCCTCAATCTCCCCGCTGGCTATTACg                                                               <  2:68794/242‑1 (MQ=255)                                                                                                                                                                    gCAGATTATCCCATCGCCGCCTCAATGATCACCGAGTTCCCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGACGCGTCCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTTGCGCGGCGCTCCCCTGTTTGTTGAGTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGc                                                       <  1:240044/250‑1 (MQ=255)                                                                                                                                                                       gATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGt                                                   <  2:120805/251‑1 (MQ=255)                                                                                                                                                                       aaTTATCCCATCGCCACTTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCTATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGt                                                   <  2:477456/250‑1 (MQ=255)                                                                                                                                                                            tCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTaaa                                                >  1:421322/1‑249 (MQ=255)                                                                                                                                                                             cccAACGCTACCTCAATGATCACCGAGGTCGCCAGTTCCCGGCAGCGGGCGTTTGCCATCAGCTTTATCGCCGCCATTTGGTATGTCGGCGCGACCTGCGCCGATCTGGGCTGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGACGAATCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAaga                                             <  2:407784/251‑1 (MQ=255)                                                                                                                                                                                  cGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTg                                          <  2:250822/249‑1 (MQ=255)                                                                                                                                                                                        cTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGCTGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTTTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGGGTGCGAag                                     >  2:425862/1‑248 (MQ=255)                                                                                                                                                                                                            ccAGTCCCCGTCAGCGGGCGTTTTCCATCAGCTGTAGCGCCGCCATGTGGGATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGttt                 <  2:310118/248‑1 (MQ=255)                                                                                                                                                                                                            ccAGGACCCGTCAGCGGGCGTTTTCCATCAGATTTATCGACGCCATGTGGTATGTCGGCGCGACATGCGCCGATCTTGTCGGAGACTGGCTTTATGATGTGGAAGGCGGCTGGTGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGACTTTGATTGGTCGATTCGAACGGCCTGAATCTCCCAGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGAGAAGAGATGATGATAAAACTGTTTGGc              <  1:1836/251‑1 (MQ=255)                                                                                                                                                                                                              aGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCg             >  1:234556/1‑250 (MQ=255)                                                                                                                                                                                                                  cccGTCAGCGGACGATTTCCATCAGCTTTATCGCCGCCATGTGGTATGTCGGCGCGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGTTTTTGCTTGGTCGATTCGCACTGCCTGAATCTCCCCGCTGGTTATTACGCAAGGGGCGAGTAAAAGAGTGCGAAGAGATGATGATAAACCTGTTTGGCGTACCg        >  2:458954/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCGGGCTTTTTCCATCAGTTTTATCGCCGCCATGTGGGATGGCGGCGGGACCTGCGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGATGAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCt  <  2:136581/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                  CGTTGGCACATCGCTGTTTGGTTATATTTCCGATAAAGTCGGACGGCGCAAAATGTTCCTCATTGATATCATCGCCATCGGCGTGATATCGGTGGCGACGATGTTTGTTTCATCCCCCGTCGAACTGTTGGTGATGCGGGTACTTATCGGCATTGTCATCGGTGCAGATTATCCCATCGCCACCTCAATGATCACCGAGTTCTCCAGTACCCGTCAGCGGGCGTTTTCCATCAGCTTTATTGCCGCGATGTGGTATGTCGGCGCGACCTGTGCCGATCTGGTCGGCTACTGGCTTTATGATGTGGAAGGCGGCTGGCGCTGGATGCTGGGTAGCGCGGCGATCCCCTGTTTGTTGATTTTGATTGGTCGATTCGAACTGCCTGAATCTCCCCGCTGGTTATTACGCAAAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCT  >  NC_000913/46007‑46472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑