Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	27,367	A→C	A25A (GCA→GCC)	rihC →	ribonucleoside hydrolase RihC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	27,367	0	A	C	100.0%	151.3 / NA	50	A25A (GCA→GCC)	rihC	ribonucleoside hydrolase RihC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/22);  total (28/22)

TGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCG  >  NC_000913/27121‑27610                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tGCAGCAGCGGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCcccc                                                                                                                                                                                                                                                  <  2:505315/250‑1 (MQ=255)        gcTGGGCGGTGGTGAGGCCATTCCGATGGAAGGCCGTGAAGAAACCATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCCTTAGCAGCCTAAGTTATGCGCAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGCAAACATGCGTTTCCCTATCTTCCTCGCTACTGACACCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAAATCgc                                                                                                                                                                                                                                          >  2:31732/1‑250 (MQ=255)        gcTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCg                                                                                                                                                                                                                                           >  1:239659/1‑250 (MQ=255)                    tgAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACtgat                                                                                                                                                                                                                              >  1:418307/1‑251 (MQ=255)                               cGCTGGAAGGTCGTGAAGAAAACATTGTTTTCTAAGTGCCGAAAGAGCTGTGTGGCGAGATTCGTGAAGTCGATTAAGGCATTAGCAGCTTAAGGTATGCGAAAATGCAGGTCTTGTTACCGGCATTTTTGATGGAGAAAACATGCGTTTACCTTTCTTCCTCGATCCTGACCCCGTCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCATCTGATGACCACCGTcg                                                                                                                                                                                                                   <  2:290213/251‑1 (MQ=255)                                        gCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGACATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGCTAATg                                                                                                                                                                                                          >  1:279941/1‑251 (MQ=255)                                             gaagaaAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCg                                                                                                                                                                                                     <  1:393424/251‑1 (MQ=255)                                                          ttttCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGACGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACt                                                                                                                                                                                         >  2:133424/1‑250 (MQ=255)                                                          tttgcGAAGGGCCGAAAGAGCTGCGTGTCGATATTCTTGAAGTCAATTAAGTCAGTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGAGTGTTGCCCCCGAACTCGACCTGCAACTGATGACCGCCTTCGCGGGTAATGTCTCGGTTGAGAAAACt                                                                                                                                                                                         <  2:70851/246‑1 (MQ=255)                                                               gAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTACGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCACCTGATGACCACCGTCGCGGGAAATGTCTCGGTTGAGAAAACTAccccc                                                                                                                                                                                   >  2:346352/1‑249 (MQ=255)                                                                  gttgagaaaggggtgagggtCGATATTCGTGAATGCGATTAAGTCATTAGCAGCCTAAGTTATGAGAAAATGCCGGTCTTGTTACAGGCATGTGTTATGGAGAAAACATGCGTGTACCTATCTTCCTCGATATGGACCCCGGAATGTACGATGCCGGCGCCATCGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGAGGGTAATGCTTCGGTTGAGAAAACTACCCGCAAt                                                                                                                                                                                <  2:350391/233‑1 (MQ=255)                                                                    gcctaaaGAGCTGCGTGTCGATATTCGTGAAGGCGATTAATTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGc                                                                                                                                                                              <  1:160054/247‑1 (MQ=255)                                                                       gAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCt                                                                                                                                                                           >  1:399381/1‑251 (MQ=255)                                                                         aaGAGCTGCGAGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGCTTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGCGACAACTACCCGCAATTccccgc                                                                                                                                                                         >  2:432266/1‑248 (MQ=255)                                                                           gagCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATTCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCaa                                                                                                                                                                       <  1:55152/251‑1 (MQ=255)                                                                            agCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAAc                                                                                                                                                                      <  1:308534/251‑1 (MQ=255)                                                                                    gtCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACATGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCAt                                                                                                                                                              >  1:485714/1‑251 (MQ=255)                                                                                          atccGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTgg                                                                                                                                                        >  1:107227/4‑251 (MQ=255)                                                                                             cGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAAt                                                                                                                                                     >  2:372211/1‑251 (MQ=255)                                                                                                gAAGTCGATTAAGGCTTTAGCAGCCAAATTGATGCGAAAATGCTGGTTTTGTCTCCGGCATTTTTTATGGAGAAAACATGCGTTTTACTATCGTCCTCGATATGGTCCCCGGCATCTACGATGCCGGCGCAATTGACGCTGCGATTTTTGACCCCGAACTCGACCTGCAACTGATGACCACCGGCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCg                                                                                                                                                  <  2:367727/251‑1 (MQ=255)                                                                                                   gTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGgag                                                                                                                                               <  1:439536/251‑1 (MQ=255)                                                                                                      gATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGCTGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACCGCAGCATTTCTGGAATGCGGAAAtt                                                                                                                                            >  2:472094/1‑251 (MQ=255)                                                                                                        ttAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTcc                                                                                                                                          >  1:140709/1‑251 (MQ=255)                                                                                                           aGTCATTAGCAGCCTAAGTTATGCGAAACTGCCGGTCTTGTTACCGGCATTTTTTATGGGGCAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATAACAACCGTAGCGGGTAATGACTCGGTTGATAAAACTACCCGCAATGCCCTACAACTGCTGCATTTCTGGAATTCGAAGATTCCGCt                                                                                                                                       >  1:260638/1‑251 (MQ=255)                                                                                                                      gcCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAgggg                                                                                                                            <  2:251305/251‑1 (MQ=255)                                                                                                                          aaggtATGCGGAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGAGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCACGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCAGCTCGCCCATGGGGCCGc                                                                                                                        >  2:324231/5‑251 (MQ=255)                                                                                                                             ttATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCCCtgt                                                                                                                     >  2:414056/1‑251 (MQ=255)                                                                                                                             ttATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTAACCGCAATGCCCTGCAACTGCTGCATTTCTGGAAGGCGGAGATTCCTCTAGCCCAAGGGGCCGcagg                                                                                                                     >  2:37632/1‑248 (MQ=255)                                                                                                                              tATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCtgtg                                                                                                                    >  2:103448/1‑251 (MQ=255)                                                                                                                                       aTGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGt                                                                                                            >  1:505702/1‑250 (MQ=255)                                                                                                                                            ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGCCCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGGGATTCCGCTCGCCCAAGGGGCCGCTGTGCCCCTGGTACgcgc                                                                                                      >  2:141003/1‑251 (MQ=255)                                                                                                                                            ggTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACgcgc                                                                                                      <  1:471330/251‑1 (MQ=255)                                                                                                                                                       cGGCATGTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACTACGGCATTGACGATGCCGTCGCCATTGCCGCAGCGATTTTTGCCCCCGAACTCGACCTGCACCTGATGACCACCGTCGCGGGTAATGCCACGGTTGAGAAAACTACACGCAATGCCATGCAACTGCTGCATTTCTGGAATGAGGAGATTCCGCGCGCCAAAGGAGCCGCTGAGACACTGGTCCGCGCCACGCGTGATg                                                                                           >  2:55503/1‑251 (MQ=255)                                                                                                                                                         gCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCg                                                                                         >  1:57572/1‑251 (MQ=255)                                                                                                                                                                 tATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCtg                                                                                  <  2:212042/250‑1 (MQ=255)                                                                                                                                                                  aTGGAGAAAACATGCGTTTCCCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCtgtg                                                                                <  1:103448/251‑1 (MQ=255)                                                                                                                                                                  aTGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCCTCtggg                                                                                >  2:160141/1‑249 (MQ=255)                                                                                                                                                                  aTGGAGAAAACATGCGTTGTCCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGACATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCATCTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTGCTGGAATGCGGAGATTCCGCTTGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCtgt                                                                                 <  2:285644/250‑1 (MQ=255)                                                                                                                                                                         aaaCATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCg                                                                         >  1:500517/1‑251 (MQ=255)                                                                                                                                                                          aaCATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATGGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTACAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGa                                                                        <  1:18364/251‑1 (MQ=255)                                                                                                                                                                               gCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCg                                                                    <  1:372211/250‑1 (MQ=255)                                                                                                                                                                                     aCCTATCTTCCTCGATACTGACACCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGAACGGCGGATCGGGGAtgg                                                             >  2:53702/1‑251 (MQ=255)                                                                                                                                                                                         aTCTTCCTCGATACTGACCCCGGCATAGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGCTGCCCACCGTCGCGGGTAATGTCTCGGTTGCGAAAACTACCCGCAATGCCCTGCAACTGCTACATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGTCCGCTGTTCCCATGGTACGAGCACCGAGTGATCCGGCATCTGTTCCCGGCGAATCGGGGAtggctgg                                                         >  2:80327/1‑251 (MQ=255)                                                                                                                                                                                                 cGATACTGACCCCGGCATTGACGAGGCCGTCGCCATTTCCGCCGCGATTTTTGCCCCCGAATTCGACCTGCAACTGATGACCACCGTCGCGGGTAAGGTCTCGGTGGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAAGGGCTGGCTACGAc                                                  <  2:384163/250‑1 (MQ=255)                                                                                                                                                                                                           cccGGCATTGACGAGGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCGGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGAGTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCAGCTGTGCACGGCGAATCGGGAATTGCTGGCTACGACTTTGTTGAGca                                       <  2:57572/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAAGCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCg                                 <  1:418937/251‑1 (MQ=255)                                                                                                                                                                                                                  ttGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGCATCGGGAATGGCTGGCTACGACTTTGTTGGGAACAACCgg                                >  2:235300/1‑250 (MQ=255)                                                                                                                                                                                                                          gCCGTCGCCATTGCCGCCGCGATTTGTGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGc                         <  1:188496/250‑1 (MQ=255)                                                                                                                                                                                                                                gCCATTGCCGCCGCGATGTTTGCCCCCGAACTCGAACTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGAGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGATTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGAt                  <  1:19672/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ttttGCCCCCGAACTCGACCTGCAACTGATGCCCCCCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCATCTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCg  <  2:130534/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ttttGCCCCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCg  >  1:142336/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCCATTGCCGCCGCGATTTTTGCACCCGAACTCGACCTGCAACTGATGACCACCGTCGCGGGTAATGTCTCGGTTGAGAAAACTACCCGCAATGCCCTGCAACTGCTGCATTTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCG  >  NC_000913/27121‑27610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑