Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 27,104 G→A Q276Q (CAG→CAA)  ispH → 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091327,1040GA98.1% 167.7 / NA 53Q276Q (CAG→CAA) ispH4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (23/29);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCC  >  NC_000913/26873‑27346
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
cACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTgc                                                                                                                                                                                                                                 <  1:269910/251‑1 (MQ=255)
    aCTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTgcagca                                                                                                                                                                                                                             <  2:272565/251‑1 (MQ=255)
        aCCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTg                                                                                                                                                                                                                         >  1:417425/1‑251 (MQ=255)
          cGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTTGCCCAGCGTATGAGCAAACGCGCGTTTTTGCTTGAAGAAGCGAAAGATATCCAGGACGAGAGGGTGAAAGAGGTTAAATGAGGCGGCGGGACAGCGGGCGAATCGGCTCCGGATATTCTTGTGCAAACTGTGGTGGCACGTTTGCAGCAGCTggg                                                                                                                                                                                                                       >  2:56489/1‑251 (MQ=255)
                    cgggaCGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCGACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGGTGCGAAAGATATCCAGGAAGGGTGGGAGAAAGAGGGTAACTGCGTGCGCGTGACTGCGGGCGCAGCAGCTCCGGATATTCTGGTGCAGAAATAGATGGCACGTTTGCACCAGCTGGGCggtggggc                                                                                                                                                                                                              >  2:55152/5‑247 (MQ=255)
                             cccTGGCAGAACAGGCGGAAGTTGTGGTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCg                                                                                                                                                                                                    <  1:315669/251‑1 (MQ=255)
                                          ggctgAAGTTGTGTTGGTGGTCTGTTCGAAAAACTCCTCCAACGCCAACCGTCTGGCGGAGCTGGCCCAGCGTATTGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGGGTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTg                                                                                                                                                                                       <  1:175914/247‑1 (MQ=255)
                                               aaGTTGTGTTGGTGGTCGGGTCGAAAAACTCCTCCAACTCCAACCGGCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGGCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTgaagaa                                                                                                                                                                                  <  2:299140/251‑1 (MQ=255)
                                                    gtgtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGGGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGGGGTGAAGCCATTCCGCTGGAGGGCCGTGAAGAAAACAt                                                                                                                                                                             >  2:471330/1‑251 (MQ=255)
                                                    gtgtTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCC‑AGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACAtt                                                                                                                                                                            >  1:505315/1‑251 (MQ=255)
                                                         ggtggtCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGttt                                                                                                                                                                        <  1:489256/251‑1 (MQ=255)
                                                         ggtggtCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGGTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGtt                                                                                                                                                                         <  1:483118/250‑1 (MQ=255)
                                                              tCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGa                                                                                                                                                                    >  1:113110/1‑250 (MQ=255)
                                                               cGGTTCGAAAAACGCCTCCAACTCCAACCGTCTGGCGGAGCTGGCTCAGCGTATGGGCAAACGCGCGGTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAg                                                                                                                                                                  <  1:227267/251‑1 (MQ=255)
                                                                         aaCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGaa                                                                                                                                                                                                             >  2:90393/1‑198 (MQ=255)
                                                                         aaCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGaa                                                                                                                                                                                                             <  1:90393/198‑1 (MQ=255)
                                                                          aCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAAtgt                                                                                                                                                                                                                                               >  2:411429/1‑163 (MQ=255)
                                                                          aCTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAAtgt                                                                                                                                                                                                                                               <  1:411429/163‑1 (MQ=255)
                                                                           ctcctcCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGCAAACATTGTTTTCGAAGTGCCGAAag                                                                                                                                                         >  2:181183/1‑248 (MQ=255)
                                                                           ctcctcCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAag                                                                                                                                                         <  1:181183/248‑1 (MQ=255)
                                                                             ccgccaactccaacCGTCGGGCGGGGCTGGCTCTGCGTATGGGCAAACGGTGGTTTTTGATTGTCGATGCGAAAGATATCCAGGAAGAGTGGGTGAAATAGGTTAGATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGGGGTGGCACGTTTGCAGCATCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTGGGCGAAGTGCCGAAAGAGc                                                                                                                                                      <  2:464057/246‑1 (MQ=255)
                                                                                 caactccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGGGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCAGTGAAGAAAACATTGTTTTCGAAGTGCCCGAAGAGCTGCgtg                                                                                                                                                >  2:418937/1‑251 (MQ=255)
                                                                                     tccaacCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCg                                                                                                                                             >  1:212042/1‑250 (MQ=255)
                                                                                      ccaacCGTCTGGCGGAGCTGGCACAGCGTATGGGCAAACGCGCGTGTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCg                                                                                                                                             <  2:104503/249‑1 (MQ=255)
                                                                                            gTCTGGCGTAGCTGGCCCAGCGGATGGGCAAACGCGCGTGTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAATAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCACCGGATAGTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTTAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCg                                                                                                                                      <  1:334488/250‑1 (MQ=255)
                                                                                            gTCTGGCGGAGCTGGCCCAGCGTCTGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGACAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGaa                                                                                                                                                                                                             >  2:178377/1‑179 (MQ=255)
                                                                                            gTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGaa                                                                                                                                                                                                             <  1:178377/179‑1 (MQ=255)
                                                                                            gTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGATGCGTGTCGATATTCGt                                                                                                                                     <  1:229468/251‑1 (MQ=255)
                                                                                                     ggCTGTCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCATCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGAt                                                                                                                            <  1:318880/250‑1 (MQ=255)
                                                                                                      gCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGAtt                                                                                                                           <  2:166976/251‑1 (MQ=255)
                                                                                                                cGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTag                                                                                                                 <  2:113315/251‑1 (MQ=255)
                                                                                                                    tGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTagcagc                                                                                                             >  1:130534/1‑251 (MQ=255)
                                                                                                                      ggCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCt                                                                                                           <  1:311554/251‑1 (MQ=255)
                                                                                                                       gCAAACGCGCGTTTTTGATTGACGATGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTa                                                                                                          <  2:123523/251‑1 (MQ=255)
                                                                                                                                               aTGCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTg                                                                                  <  1:56489/251‑1 (MQ=255)
                                                                                                                                                 gCGAAAGATATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGtt                                                                                <  1:279133/251‑1 (MQ=255)
                                                                                                                                                             cAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGTAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCAttttt                                                                    >  1:107833/1‑251 (MQ=255)
                                                                                                                                                             cAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCAttttt                                                                    >  1:251305/1‑251 (MQ=255)
                                                                                                                                                                 aaGAGTGCGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAACTGCCGGTCTTGTTACCGGCATTTTTTATg                                                                >  2:348497/1‑251 (MQ=255)
                                                                                                                                                                           gAAAGAGGTTAAATGCGTCGGCGAGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACAt                                                      >  2:41872/1‑251 (MQ=255)
                                                                                                                                                                            aaaGAGGTTAAATGCGTCGGCGAGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGGGGCACGTTTGCAGCAGCTGGGCGGGGGTGAAGCCATTCCGCTGGAAGGCCGTAAAGAAAACATTGTTTTCGAAGTGACGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGTTTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTAGTGTAACCGGCATTTTTTATGTATAAAACATg                                                     >  2:348339/1‑251 (MQ=255)
                                                                                                                                                                             aaGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGc                                                    <  1:144379/251‑1 (MQ=255)
                                                                                                                                                                                agGTTAAATGCGTCGGCGTGAATGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGACGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGACAGAGCTGCGTGTCGCTATTCGTGACGTCGATTAAGTCATTAGCAGCCTCAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGtt                                                 >  2:457642/1‑251 (MQ=255)
                                                                                                                                                                                  gtttaatgcgcacgcgttgcTGCGGTCTCATCGGCTCCGGATATTCTTGTGCAAAATGTGGTTGCACGTTTGCAGCATCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAAACATTAGCAGCCTAAGTTATGCGAAAATGCCTGTCTTGTTACCTGCATTTTTTATGGAGAAAACATGCGTTTa                                               <  1:261934/232‑1 (MQ=255)
                                                                                                                                                                                       aTGCGTCGGGTTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAt                                          <  2:417425/251‑1 (MQ=255)
                                                                                                                                                                                       aTGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTAt                                          <  1:270566/251‑1 (MQ=255)
                                                                                                                                                                                                          ggCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATCCTGAcccc                       >  2:18364/1‑251 (MQ=255)
                                                                                                                                                                                                          ggCGCATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGAcccc                       >  1:306442/1‑251 (MQ=255)
                                                                                                                                                                                                            ggcATCGGCTCCGGATATTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCgg                     <  2:189434/250‑1 (MQ=255)
                                                                                                                                                                                                                       cGGATATTCTGGTGCAAAATGGGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATg          <  1:476932/251‑1 (MQ=255)
                                                                                                                                                                                                                           tatTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGt      >  1:294536/1‑251 (MQ=255)
                                                                                                                                                                                                                            atTCTGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGACATTCCACTGGAAGGCCGTGAAGAAAACATTGTTATAGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGCAATCATGCGTTTACATATCTTCCTAGATACTGACACCGGAATTAACGAtcgcgtc     >  1:292170/1‑245 (MQ=255)
                                                                                                                                                                                                                                tGGTGCAAAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGcc  >  2:65542/1‑250 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
CACGACTAACCGTCAGGAAGCGGTACGCGCCCTGGCAGAACAGGCGGAAGTTGTGTTGGTGGTCGGTTCGAAAAACTCCTCCAACTCCAACCGTCTGGCGGAGCTGGCCCAGCGTATGGGCAAACGCGCGTTTTTGATTGACGATGCGAAAGACATCCAGGAAGAGTGGGTGAAAGAGGTTAAATGCGTCGGCGTGACTGCGGGCGCATCGGCTCCGGATATTCTGGTGCAGAATGTGGTGGCACGTTTGCAGCAGCTGGGCGGTGGTGAAGCCATTCCGCTGGAAGGCCGTGAAGAAAACATTGTTTTCGAAGTGCCGAAAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCGGCATTTTTTATGGAGAAAACATGCGTTTACCTATCTTCCTCGATACTGACCCCGGCATTGACGATGCCGTCGCC  >  NC_000913/26873‑27346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑