Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	26,666	A→G	T130T (ACA→ACG)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	26,666	0	A	G	100.0%	168.8 / NA	53	T130T (ACA→ACG)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/29);  total (24/29)

CTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGG  >  NC_000913/26420‑26907                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  cTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATgg                                                                                                                                                                                                                                               >  1:464057/1‑251 (MQ=255)     cGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCca                                                                                                                                                                                                                                           <  1:311292/251‑1 (MQ=255)        gAGCGTGGGGCTATTTTTATTGAGCAGATTAGCGAATTACCGGACGGCGCGATCCTGATTTTCTCCTCACCCGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGAGTCTCATCGGCCACGCAGGGCATCCGGAAGTGGAAGGGACGATGGGCcagta                                                                                                                                                                                                                                        <  2:347432/251‑1 (MQ=255)         ggCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGAGCCTGATTTTCACCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATAGGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCcagtac                                                                                                                                                                                                                                       <  2:41120/250‑1 (MQ=255)            gTGGGTCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGCTTTGACGGTGTTTGGTGCCACCTGTCCGCTGGTGCGCCAAGTGCATCTGGAAGTCGCCCGCGCCAGCCGTCGCGACGAAGAGTCGAGTCTCATCGGCCACGCCGGGCATCCGGGAGTGGCAGGGACGCTGGGCcaggacagt                                                                                                                                                                                                                                    >  2:387019/1‑251 (MQ=255)                      cTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGGACAGTAACCCGGa                                                                                                                                                                                                                            >  1:104503/1‑249 (MQ=255)                               gCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATgg                                                                                                                                                                                                                 >  1:487856/1‑250 (MQ=255)                                 aGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATCCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTAt                                                                                                                                                                                                               >  1:217257/1‑251 (MQ=255)                                    ttAGGGAAGGACCGGACGGCGCGATCCTGATTGTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGAGATTTGACGGTGGTTGATGCCACCTGGCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCAGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCt                                                                                                                                                                                                             <  2:132462/250‑1 (MQ=255)                                            gTACCGGCCGTCTCGATCCTGATTTGCTCCGCACGCGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATc                                                                                                                                                                                                    <  2:107391/251‑1 (MQ=255)                                             aaCCGGACGGCGCGGTCCTGATTTTCTCCGCACAAGGTGTTTATCAGGCGGCACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCACAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGACGCAGAGTAGAATCTCATCGGCCAAGACGGGCATCCGGAAGGGGAAGGTACGGTGGGCCAGTACCGAACCCCGGCAGGGGAAATGTCACTGGTCgtat                                                                                                                                                                                                     >  2:318880/2‑246 (MQ=255)                                                 ggACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCgg                                                                                                                                                                                               <  1:359919/251‑1 (MQ=255)                                                  gACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGATGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCggg                                                                                                                                                                                              >  1:76565/1‑250 (MQ=255)                                                          gATCCTGATGTTCGCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtgt                                                                                                                                                                                      <  1:429431/251‑1 (MQ=255)                                                             ccTGATTTTCTCCGCACACGGAGTTTCTCAGGCGGCACGTAACGCAGCAAAAAGCACCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGCGACCAACGTGCATATGGAAATCGCCCGCGCAAGCCGTCGTGGCGAGCAGTCGATTCTCATCGGCAACGACGGGCCACCGGAAGTGGACGGTACGATGGGACAGGCCAGTAAAGCGGAAGGGGGCACAGATCTGGTCGAATCGCCGGACgcaagtggg                                                                                                                                                                                    >  2:503950/1‑242 (MQ=255)                                                                   tttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTg                                                                                                                                                                              <  1:12888/250‑1 (MQ=255)                                                                   tttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGAATCAGGAAGTGGCAGGAACGATGGGCCAGTAAAGGAACCCGGAAGGGGGAATGGATATGGTCGAATAGCCGGGCGGAGTGGGGAAACTg                                                                                                                                                                              >  2:430221/1‑250 (MQ=255)                                                                      ctcCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACgg                                                                                                                                                                          >  1:123523/1‑251 (MQ=255)                                                                          gtacacGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATTCCACCTGTCCGCTGGTGACCAAAGGGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACTCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaa                                                                                                                                                                      <  1:255831/249‑1 (MQ=255)                                                                             cacGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaaaaa                                                                                                                                                                   <  1:291426/251‑1 (MQ=255)                                                                                  ggtTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTGGATGCCACTTGTCCGCTGGTGACCAAAGTGCATACGGACGTCGCCCGCGCCAGCCGTAGTGGCGAAGAGTCGATTATCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGAAGATGTGTGGAAACTGACGGTCAAAAACGAag                                                                                                                                                              <  1:387019/250‑1 (MQ=255)                                                                                             cggttggTAACGAAGCAAAAAGCCGCGATTTGCCGGTGTTTGTTGCCACCTGTCCGCTGGTGGCCAAAGGGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGTATCCGGAAGTGGAAGGTACGATGGGCCAGTTCGGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTcc                                                                                                                                                   <  1:112951/245‑1 (MQ=255)                                                                                              ggTACGTAACGAAGCAAAAAGCCGCGCTTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTgg                                                                                                                                                                                                           >  1:286256/1‑194 (MQ=255)                                                                                              ggTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTATCCCGGAAGGGGGAATGTATCTgg                                                                                                                                                                                                           <  2:286256/194‑1 (MQ=255)                                                                                                    tAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGAGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGACGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATgc                                                                                                                                            >  2:327840/1‑250 (MQ=255)                                                                                                        gAAGCAAAAAGCCGCGATTTGACGGAGTTTGATGCCACCTGTCCGCTGGTGCCCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCAGGCAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGCAGGGGGACTGAATCTAGTCGAATCGCCGGACGAAGTGTGGAAACTGACGATCAAAAACGAAGAGAAGATCTCCTTTATGAccc                                                                                                                                         >  1:367727/1‑250 (MQ=255)                                                                                                             aaaaaGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCa                                                                                                                                   <  1:213172/251‑1 (MQ=255)                                                                                                                 agccgcgATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATTACCCAGACCACGCt                                                                                                                               <  1:400573/248‑1 (MQ=255)                                                                                                                  gccgcgATTTGACGGTGTTGGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTg                                                                                                                              <  2:419493/249‑1 (MQ=255)                                                                                                                    cgcgATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTc                                                                                                                            <  1:477363/251‑1 (MQ=255)                                                                                                                                      gATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGACGTGGCGAAGAGGCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAAACCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTATCCTTTATGACCCAGGCCACGCTGTCGGTGGagtcaacggtcgt                                                                                                          >  2:483118/1‑239 (MQ=255)                                                                                                                                        tGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGCGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg                                                                                                        >  1:111710/1‑251 (MQ=255)                                                                                                                                                gTCCGCTGGTGACCAAAGGGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCTAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCGCTCCTTTATGACCCAGACCACACTTGCGGTGGATGACCCGTCTGATGTGATCg                                                                                                  <  2:29302/249‑1 (MQ=255)                                                                                                                                                       ggTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTg                                                                                          <  1:233196/250‑1 (MQ=255)                                                                                                                                                             cAAAGTGCATATGGAAGTCCCCCGCGCCAGCCGTCGTGGCGAAGATTCGATTCGCATCGGCCACGCCGGGCCACCGGAAGTTGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCGGATGTGATCGACGCGCTGCGTAAAc                                                                                   <  2:279566/251‑1 (MQ=255)                                                                                                                                                              aaaGTGCATATGGAAGTCGCCCGCTCCAGCCGTCTTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACg                                                                                  <  1:152201/251‑1 (MQ=255)                                                                                                                                                                       atGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTCTCTGGTCGAATCGCCGGACGATGAGTGGAAACTGAAGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGACGGTGGATGACACGGCTGCTGTGATCGCAGCCCTGCGTAACCGCTTCCCGa                                                                          >  2:439536/1‑250 (MQ=255)                                                                                                                                                                       atGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGGCCACGCTGTCGGTGGATGACACGTCTGATGTGATCGCCGCGCTGCGTAAACGCTTCCCGa                                                                          >  1:290213/1‑250 (MQ=255)                                                                                                                                                                       atGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGCCGCGCTGCGTAAACGCTTCCCGa                                                                          >  1:272565/1‑250 (MQ=255)                                                                                                                                                                         ggAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGaaa                                                                        >  1:113315/1‑250 (MQ=255)                                                                                                                                                                                  ccGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTc                                                              <  1:157279/251‑1 (MQ=255)                                                                                                                                                                                             gtcGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGGGGAAGGTACGATGGGCCAGTACAGTCACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAg                                                     >  2:102115/3‑249 (MQ=255)                                                                                                                                                                                                    cGAAGAGTCGATTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAAGCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCt                                            <  1:94350/251‑1 (MQ=255)                                                                                                                                                                                                              aTTCTCATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCAc                                  <  1:382074/251‑1 (MQ=255)                                                                                                                                                                                                                   cATCGGCCACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTa                             <  1:375243/251‑1 (MQ=255)                                                                                                                                                                                                                         ccACGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTc                       <  1:395002/250‑1 (MQ=255)                                                                                                                                                                                                                          aaCGCCGGGCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGACGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCACGCCCCCGCTGTCGGTGGATGACAAGTCTGATGTGATCGACGCCCTGGGGAAACGATTCCCGAAAATTGTCGGTCCGCGCCAAGCTGACATCTGCTACGCCACGACTAACCggc                       >  2:315669/2‑248 (MQ=255)                                                                                                                                                                                                                                gggCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCCCGACTACCCGTCAGGAAGc                >  2:335955/1‑251 (MQ=255)                                                                                                                                                                                                                                gggCATCCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGAACCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCCGCGTAAACGCCTCCCGAAAATTGTCGGTCCGCGCCAAGAGGACATCTGCTACGCCACGACTAACCGTCAGGAAGc                >  2:393424/1‑251 (MQ=255)                                                                                                                                                                                                                                    atccGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGGCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTa            <  1:36870/249‑1 (MQ=255)                                                                                                                                                                                                                                      ccGGAAGGGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGCCCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGCCGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTAcg          <  1:276261/251‑1 (MQ=255)                                                                                                                                                                                                                                        ggAAGTGGAAGGAACGATGGGCCAGTACAGGAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGGGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGCCGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGAGCAAAGATGACATCGGATACGCAACGCATAACCGTCAGGAAGCGGTAcgcg        >  2:144379/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGGATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCGCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAATGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTgg  <  2:223588/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  CTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGACATCTGCTACGCCACGACTAACCGTCAGGAAGCGGTACGCGCCCTGG  >  NC_000913/26420‑26907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑