Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,935	T→G	S849A (TCG→GCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,935	0	T	G	94.4%	207.1 / ‑0.8	71	S849A (TCG→GCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (31/36);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCCG  >  NC_000913/24694‑25178                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGaaa                                                                                                                                                                                                                                              >  2:106345/1‑250 (MQ=255)   tCACCGCTGATGAAGGGTGGGGCTACCTGCCGGGCGAACGGGAAAGATACGTCTTCACCGGTGAGGGGTACGAAGGCCTGTTTGGTATGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACt                                                                                                                                                                                                                                            <  2:114306/250‑1 (MQ=255)        gCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACcgc                                                                                                                                                                                                                                      <  1:278272/251‑1 (MQ=255)           gatgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGCACTGGCGGCGAAACTGACCGCGCt                                                                                                                                                                                                                                   >  2:379165/1‑251 (MQ=255)              gaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTggg                                                                                                                                                                                                                                <  1:375180/251‑1 (MQ=255)                aGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCg                                                                                                                                                                                                                              <  2:153291/251‑1 (MQ=255)                        gCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAttc                                                                                                                                                                                                                      >  1:39325/1‑250 (MQ=255)                            cccggcGGGCGAACGTGAAAAATCCGTCGTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAt                                                                                                                                                                                                                  <  2:439341/246‑1 (MQ=255)                               gCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTGGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTg                                                                                                                                                                                                               <  2:57514/251‑1 (MQ=255)                                   ggCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTctt                                                                                                                                                                                                           <  1:255784/251‑3 (MQ=255)                                    gCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttg                                                                                                                                                                                                          <  2:170322/251‑4 (MQ=255)                                     cGAACGTGAAAAATGCGTCTTCACCGGTGAGCGGTACGAAGGCCTGTTTGGTCTGGCGGACAGTGAAGCAATGAACGATGCGATCTGGGACGCGCTGTTGAAAGAGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGAGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAAAACCGGAACTGGCGGCTAAAATGACCGCGCTGGGCGAtagaatacgtattggcttgt                                                                                                                                                                                                         >  2:355796/1‑232 (MQ=255)                                      gAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGtt                                                                                                                                                                                                        <  1:346085/251‑1 (MQ=255)                                      gAACGTGAAAAATACGTCTGCACCGGTTGGTGGTGCGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGtt                                                                                                                                                                                                        <  2:442657/251‑1 (MQ=255)                                          gTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAATGACCGCGCTGGGCGATGAATTACGATTTGTCttgtt‑gccc                                                                                                                                                                                                   >  2:395204/1‑248 (MQ=255)                                              aaaaTACGTCTTCACTGGTGAGTGGTTCGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGGGATGAATTACGATTTGTCTTGTT‑GACCTCCg                                                                                                                                                                                               <  2:421603/251‑1 (MQ=255)                                              aaaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCg                                                                                                                                                                                               <  2:166254/251‑1 (MQ=255)                                               caagaCGTCTTCACCGGTGAGTGGTAAGAAGGCCTGTTAGGTATGGCCGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACACAGTCATAGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGATGGAAGCGGCAGTAAACTTGTATACAGAACAGGAACTGGCGGCGAAAATGAACGAGCTGGGCGAGGAATTACGATTTGTCTTGTT‑GACCTACgg                                                                                                                                                                                              >  2:41218/5‑251 (MQ=255)                                                      tCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTAcc                                                                                                                                                                                       >  1:78723/1‑251 (MQ=255)                                                      tCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTAcc                                                                                                                                                                                       >  1:128780/1‑251 (MQ=255)                                                         gcACCGGTGAGTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTAcc                                                                                                                                                                                       <  2:392723/247‑1 (MQ=255)                                                            ccGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGAGCGGCGAAGTGAACCAAGTCATTGAGCCAGCGCGTGCCGCCAAGAAAGTGGGTGGCTCGATGGAAGCGGACGTAACCTGGTATGCCGAACCGGAACTGGCGGCGGAACATACCGCGCTGGGCGCAGAATTACGATTGGTCTTGTT‑GTCGTCCggggcgacggtcgc                                                                                                                                                                                  >  2:267092/1‑238 (MQ=255)                                                             cGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGTACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAg                                                                                                                                                                                <  1:330562/251‑1 (MQ=255)                                                                 gAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCGGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTTACTTGTTTGAC‑TCCGGCGCTACCggttgaggct                                                                                                                                                                             >  2:40289/1‑241 (MQ=255)                                                                 gAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACta                                                                                                                                                                            <  1:23396/251‑1 (MQ=255)                                                                  aGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGAGGGTGGCTCGCTGGAAGCGGCAGTAACAGTGTATGAAGAACCGGAACTAGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGCTTTGTCTTGTT‑GACCTCAGGCGCTCCCGTTGcggaatat                                                                                                                                                                           >  2:408560/1‑244 (MQ=255)                                                                    tGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGCTGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACggt                                                                                                                                                                                                                  >  2:70450/1‑209 (MQ=255)                                                                    tGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAt                                                                                                                                                                                                                  <  1:70450/211‑1 (MQ=255)                                                                      gTACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGGGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAacg                                                                                                                                                                       <  2:25592/251‑1 (MQ=255)                                                                           aaGGCCCGTTTGGTCTGGCAGACAGGGAAGCTATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCa                                                                                                                                                                  <  2:109675/251‑1 (MQ=255)                                                                            aGGCATGTTTGGTCTGGCAGACAGTGAAGCAATGAATGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAATAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCAc                                                                                                                                                                 <  2:54571/251‑1 (MQ=255)                                                                             ggCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCAcc                                                                                                                                                                <  2:178712/251‑1 (MQ=255)                                                                              gCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCGGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACct                                                                                                                                                               <  2:478911/251‑1 (MQ=255)                                                                                 tGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATg                                                                                                                                                                                                                           <  1:31414/189‑1 (MQ=255)                                                                                        tcTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGCCGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGAGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGCCTCTAACGACGCACCTGCTGATGCTc                                                                                                                                                     >  1:42992/2‑251 (MQ=255)                                                                                           ggCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTTCCGTTGCAGTCTATAACGACGCACCTGCTGATGCTca                                                                                                                                                    <  1:199089/249‑1 (MQ=255)                                                                                           ggCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagc                                                                                                                                                  <  2:383191/251‑1 (MQ=255)                                                                                                    tGAAGCAATGTACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGACGTGAACAAAGTCATAGAGCCAGCGCGGGCCGACAAGAAAGTGGGTGGCTAGATGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGCTTTGTCTTGTT‑GACCTCAGGAGCTACCGTGGCCGACAATAACGACGCACCTGCTGATGCTAAGCAGAGCgc                                                                                                                                           >  2:326395/1‑248 (MQ=255)                                                                                                      aaGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGTTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGt                                                                                                                                        >  1:100406/1‑250 (MQ=255)                                                                                                         caaTGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTc                                                                                                                                    <  2:42992/249‑1 (MQ=255)                                                                                                           aTGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGCTGCTCAGCAGAGCGCAGTCCTCa                                                                                                                                   >  2:246051/1‑250 (MQ=255)                                                                                                                   tGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTg                                                                                                                           >  1:203752/1‑250 (MQ=255)                                                                                                                          tGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTcgc                                                                                                                   >  1:102297/1‑251 (MQ=255)                                                                                                                           gggACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTcg                                                                                                                    <  1:106631/249‑1 (MQ=255)                                                                                                                                 tgCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGGGGCTCGCTGGAAGCGGCAGTAACCTGGTATGCAGAAACGGACCTGGCGGCGGTACTGGCCGCGCGTGGCGATGAATTACGATTTGTATTTTT‑GACCTCCGGCGCTACCGTTGCAGGCTATAACGACGCAACTGCTGATGCTCAGCCGAGCGAAGTACTCCACGGGCTGCAAGTAGCGTTGAGt                                                                                                            >  2:134061/2‑251 (MQ=255)                                                                                                                                   cggtggAAAGGGCGTGGCGAAGTGAACCAAGTCATGGAGCAAGCGCGTGACGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTTGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTTGCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCTGCAGAGCGAGGTACTCAAAGGGCTGAAAGTCGCGTTGAGTaa                                                                                                          <  2:336199/246‑1 (MQ=255)                                                                                                                                     gTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCCTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTaaa                                                                                                         <  1:408560/250‑1 (MQ=255)                                                                                                                                             tGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGCTGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTTGGTAAAGCCGAGGGt                                                                                                >  2:13077/1‑251 (MQ=255)                                                                                                                                                              aaaGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgctg                                                                               <  1:502767/251‑1 (MQ=255)                                                                                                                                                              aaaGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGAGTTGAGTAAAGCCGGAGGTGAGAAGGGCCAACgctg                                                                               >  2:113195/1‑251 (MQ=255)                                                                                                                                                                aGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACgctgct                                                                             >  1:444774/1‑251 (MQ=255)                                                                                                                                                                          cAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCCAAAGGCTGAAAGTCGCGTTTCGTAAAACCGAAGGTGAGAAGGGCCAACGCTGCTGGCCCTacac                                                                   >  2:189876/1‑251 (MQ=255)                                                                                                                                                                           aaGCGCGGGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAAGTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACAcc                                                                  <  2:83138/251‑1 (MQ=255)                                                                                                                                                                             gcgcGTGCAGACAAGAAAGGGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCa                                                                <  2:409592/251‑1 (MQ=255)                                                                                                                                                                                 gTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGAt                                                            <  1:106345/251‑1 (MQ=255)                                                                                                                                                                                  tGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCg                                                                                                                                                                                               >  1:68793/1‑119 (MQ=255)                                                                                                                                                                                  tGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCg                                                                                                                                                                                               <  2:68793/119‑1 (MQ=255)                                                                                                                                                                                    ccGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGGGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTc                                                         >  2:418357/1‑251 (MQ=255)                                                                                                                                                                                                        tCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGTTTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCACCAGAGCGAAGTAATCAAAGGGCTGAAAGTCGGGTTGAGTAAAGCCGAAGGTGAGAGATGCCCACGCTGCTGGCACCACCCCCAGGATGTCGGCAAGGTGGCGGCACACGc                                     >  2:378299/1‑251 (MQ=255)                                                                                                                                                                                                              gAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAAt                               >  1:431894/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGc                        <  2:100406/251‑1 (MQ=255)                                                                                                                                                                                                                          aCCTTGTATGCCGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGCTTTGTCCTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATCACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCCCTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGACGCtg                   >  2:456248/1‑251 (MQ=255)                                                                                                                                                                                                                          aCCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCtg                   <  1:456248/251‑1 (MQ=255)                                                                                                                                                                                                                               gtattaaGAACGGGAACTGTCGGCGAGACTGCCCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCGCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCGGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCa              <  2:233292/245‑1 (MQ=255)                                                                                                                                                                                                                               gTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGGGGCCGCttggtc               >  2:495078/1‑245 (MQ=255)                                                                                                                                                                                                                                tATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAGGTGCCCACGCTGCTGGAACTACACCCAGGATGTCGGCCAGGTGGCGGAAAACGCAGAAATCTGCGGCCGCTGAGTc               >  2:182197/1‑249 (MQ=255)                                                                                                                                                                                                                                 aTGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAg             <  2:298451/250‑1 (MQ=255)                                                                                                                                                                                                                                   gcagcaCCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTAAGATTTGTCCTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTACAGCCGCAGGTGAGCAGTGCCCACCATGCTGGCACTACACCCAGGATGTCGGCAAGGTGTCGGAACACGCATAAATATGCGGCCGcctggtacgca           >  2:94457/1‑240 (MQ=255)                                                                                                                                                                                                                                        aCCGGCACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGGTGATAGGCGCGTTGTGTAAAGCCGAAGGTGCGAAGTGCCCACGCTGCTGGCACTCCACCCAGGAAGTCGGCGAGGGGGCGGAACACGCCGAAATCTGCTGCGGCTGGGTCAGAAACggcg     >  2:115823/1‑248 (MQ=255)                                                                                                                                                                                                                                        aCCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTT‑GACCGCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGAGTTGAGTAAAGCCGAAGGTGAGAAGTGCTCACGCTGCTGGCACTACACCCAGGAGGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGTCCGCTGTGTCAGCAACGTCg     <  1:197613/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggtaCTGGCGGCGAAACTGACCGCGCTGGGCGATGCATTACGATTTGTCTTGTT‑GTCCGCCGGCGCTACCGTTGAAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGATCTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCAGATGGTGAGAAGTGCCCACGCTGCTGGCGATAACCCCAGGATGTCGGCAGGGGGGCGGAAACCCCCGAAATATGAGGCCGCTGTGTGGGCAACGTCGCcg  >  2:296191/4‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTT‑GACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGAGCGAAGTACTCAAAGGGCTGAAAGTCGCGTTGAGTAAAGCCGAAGGTGAGAAGTGCCCACGCTGCTGGCACTACACCCAGGATGTCGGCAAGGTGGCGGAACACGCAGAAATCTGCGGCCGCTGTGTCAGCAACGTCGCCG  >  NC_000913/24694‑25178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑