Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,217	T→C	N609N (AAT→AAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,217	0	T	C	98.3%	174.7 / NA	61	N609N (AAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (27/32);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTG  >  NC_000913/23988‑24462                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGAAATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACAACGTTTCGCCGCAGGAtgtg                                                                                                                                                                                                                                  >  1:55187/1‑251 (MQ=255)  ttGGTTTGACTCCGGATCTACCCCCTCTTCTGTTGTTGACTTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAAGGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                                                                                                                  <  2:81624/249‑1 (MQ=255)  aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                                                                                                                                                                 <  2:85138/251‑1 (MQ=255)       tgtaCTCCGTATCGACCCACTCTTCTGTTGTTGATGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGGTCAGACCAGCACCGTGGTTTGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACTGCTTGACCGTGGATGTGCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGaa                                                                                                                                                                                                                             <  1:30227/247‑1 (MQ=255)         gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                                                                                                                                                           <  1:420372/250‑1 (MQ=255)          aCTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaaa                                                                                                                                                                                                                         <  1:242477/251‑1 (MQ=255)            tCCGGATCGTCCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAATGTTCTGACCAGCACCGGGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCTCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGTATTGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTTCGCCGTCGGATGTGATGATCAAAc                                                                                                                                                                                                                        <  2:324802/250‑1 (MQ=255)             ccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg                                                                                                                                                                                                                      <  2:391510/251‑1 (MQ=255)                         aCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATAtt                                                                                                                                                                                                          <  1:415489/251‑1 (MQ=255)                             ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCCGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGGTGTGATGAACaca                                                                                                                                                                                                                         >  2:83466/1‑230 (MQ=255)                             ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaaa                                                                                                                                                                                                                         <  1:83466/232‑1 (MQ=255)                                      tgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCATCTCCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCATGGCCGCAAGATTTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGTCGCGGATATTCTGCGTCTgtgg                                                                                                                                                                                              <  1:453039/250‑1 (MQ=255)                                               tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGGGGATGGTTCATGTCTTACCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCATTACCGTGGATGGACAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAAAAACTGGGCGAGGATAATCTGCGTCTGGGGGGGGAATCa                                                                                                                                                                                     >  2:140976/1‑250 (MQ=255)                                                          ccGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCCCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACAcc                                                                                                                                                                         <  2:326704/251‑1 (MQ=255)                                                                   cAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATg                                                                                                                                                                >  1:27385/1‑251 (MQ=255)                                                                    aGCGGACATGTAGCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATgg                                                                                                                                                               <  2:119022/251‑1 (MQ=255)                                                                       ggACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAACTCCATCGGCACCACCGTTTCCCCGCAGGATGTGATGAACAAACTGGGCGCGGCTATTCTGCGTTTGTGGGTGGCATCAACAGACTACACCGGTGAAATGGCCg                                                                                                                                                            >  2:238099/1‑251 (MQ=255)                                                                                      aGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCCTCAACCGACTACACCGGTGAAATGGCCGTTTCTGACTAGATcc                                                                                                                                             >  1:259952/1‑251 (MQ=255)                                                                                             gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACg                                                                                                                                      <  1:131690/251‑1 (MQ=255)                                                                                              aCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAACGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                                                                                                                     >  1:279165/1‑251 (MQ=255)                                                                                               ccagcaccgtggttgggtcttGTCTTCCCTAATGATCTCCACCGCGATGAAAGTCAAAGCGCCGTATCGTCAGGTATTGACCCACGGCTTTACCTTGGATGGTCAGGGCCGCAAGATGTCTACATCCATCGGCAACACCGGTTCGCCGCAGGAGGTGATGAACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACTCCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtg                                                                                                                                    <  1:67218/231‑1 (MQ=255)                                                                                                    aacgcgGTTGGTTCATGTCTTCCCTAATGATCTCCACAGCGATGTAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGAATGTCAGGGCCGCAAGCTGTCTAAATCCATCGGAAAAACCGTTTCGCCGCAGGTAGTGCTGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATAAACCGACTACACCGGTGACATGACCGTTTCTggagcgatcttgacacaggtggcc                                                                                                                               >  2:377586/3‑228 (MQ=255)                                                                                                     ccgtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCg                                                                                                                              >  2:91736/8‑251 (MQ=255)                                                                                                       gtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                                                                                                            <  1:470000/246‑2 (MQ=255)                                                                                                       gtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGGACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACTCCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATGCTTCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                                                                                                            <  2:46217/246‑2 (MQ=255)                                                                                                           ttgggtCATGTCTTCACTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACAACGTTTCGCCGCAGGATGTGATGAAAAAAATGGACGCGGATATTCTGCGGCTGTGGGGGGCATCAACCGACTACACCGGTGAAATGCCCGTTTCTGACGAGTTCCTGCAACGAGCAGCCGACAGcg                                                                                                                        >  2:199089/6‑250 (MQ=255)                                                                                                             ggTTCATGTCTTCCCTAATGATCTCCACCGCGAGGAAAGGCAAAGCGTCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATCCTGAAACGAGCTGCCGACAGCTAt                                                                                                                      <  1:115812/251‑1 (MQ=255)                                                                                                                 cATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcgtc                                                                                                                  <  1:154678/251‑1 (MQ=255)                                                                                                                    gTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTATGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGCAATGGCCGTTTCTGACGAGATCCTGAAACGTACTGCCGACAGCTATcgtcgt                                                                                                                >  1:366642/1‑250 (MQ=255)                                                                                                                    gTCTGCCCTAATGATCTCCCCCGCGATGAAAGGCAAAGCTCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCATGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATcgtcgt                                                                                                                <  1:333189/250‑1 (MQ=255)                                                                                                                              aTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAacac                                                                                                     >  1:485384/1‑251 (MQ=255)                                                                                                                               tGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                                                                                                    >  2:134318/1‑251 (MQ=255)                                                                                                                                   ctcCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgcgc                                                                                                >  1:28969/1‑251 (MQ=255)                                                                                                                                   ctcCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACCCCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTACCCGTAACACcgcgc                                                                                                >  2:193783/1‑251 (MQ=255)                                                                                                                                         cgcgATGAAAGGCAAAGGGCCGTATCGTCAGGTACTGAACCACGGCTTTACCGGGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGCAACGTGATGCCGACAGCTAACGTCGTAACCGTAACACCGCGCGCTTcc                                                                                          >  2:161035/1‑251 (MQ=255)                                                                                                                                            gaggaaaggcaaaGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAAGGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCctgc                                                                                       <  2:7771/241‑1 (MQ=255)                                                                                                                                             aTGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGCAACACCGCGTGCTTCccgct                                                                                      >  1:278413/1‑251 (MQ=255)                                                                                                                                                     caaaGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCAGCGGCAACGCCGTTTCGCCGCAGGATGTGATGAACAAACGGGGCGCGGATATTCTGCGTCTGTGGTTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGGGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAAcc                                                                              <  1:247701/250‑1 (MQ=255)                                                                                                                                                       aaGCGCCGTATAGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATTGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGGAACACCGCGCGCTTCCTGCTGGCAAACCTg                                                                            >  2:309671/1‑251 (MQ=255)                                                                                                                                                          cgcCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAAc                                                                         >  1:429287/1‑251 (MQ=255)                                                                                                                                                             cGTATCGTCAGGTACTGACCCACTGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCTCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTTCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACCCCGCGCGCTTCCTGCTGGCAAACCTGAACGGt                                                                      <  1:500538/251‑1 (MQ=255)                                                                                                                                                             cGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGt                                                                      >  1:227353/1‑251 (MQ=255)                                                                                                                                                                  cGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTg                                                                  <  1:368565/250‑1 (MQ=255)                                                                                                                                                                                     gCTTTACCTTGGATGGTCAGGGCCGCAAGAGGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTg                                              <  2:108762/251‑1 (MQ=255)                                                                                                                                                                                     gCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTg                                              >  1:108762/1‑251 (MQ=255)                                                                                                                                                                                          aacGTGGTTGGTCAGGGTCGCAAGATGTCTAAATCCATCGGCAATACAGTTCAGCCGCAGGATTTGATGAACAAACTGGGCGCGGATATTTTGCGTCTGTGGGTGGCATCGACCGACTACGCCGGTGAAATGGCTGTTTCTGGCGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTTTCCGTAACACAGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTgtcacc                                         <  2:174440/249‑6 (MQ=255)                                                                                                                                                                                             gTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCgg                                       >  1:439341/1‑250 (MQ=255)                                                                                                                                                                                               ggATGGTCAGGGCCGCAAGATGTCTAAATCCATCGCCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTCCACCGGTGAAATGGCCGTTTCTGACGAGATCCACAAACGTGCTGCCGACAGCCATCGTCGTCTCCGTAACACCGCGCGCTACCTGCTGCCAACCGTGACCGGCTTTGATCCAGCaaacgtactggcagaaccggaa                                     >  2:206450/1‑231 (MQ=255)                                                                                                                                                                                                  gggccAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAgaga                                 <  2:27385/247‑1 (MQ=255)                                                                                                                                                                                                   ggTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAt                                <  1:238099/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggt                             <  1:253612/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGAtggt                             <  1:51790/251‑1 (MQ=255)                                                                                                                                                                                                               aaGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGa                    <  1:405037/251‑1 (MQ=255)                                                                                                                                                                                                                     tCTAAATCCATCGGCCACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                                                                                                    >  1:372182/1‑165 (MQ=255)                                                                                                                                                                                                                     tCTAAATCCAGCGGAAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                                                                                                    <  2:372182/165‑1 (MQ=255)                                                                                                                                                                                                                           tCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGCAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCgg        >  1:114306/1‑250 (MQ=255)                                                                                                                                                                                                                            ccATCGGCAACCTCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACCCCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGt        <  1:298551/250‑1 (MQ=255)                                                                                                                                                                                                                             cATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTgg                     >  2:140656/1‑236 (MQ=255)                                                                                                                                                                                                                             cATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTgg                     <  1:140656/236‑1 (MQ=255)                                                                                                                                                                                                                              aTCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAgg     >  1:427426/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATAAACCGACTACACCGGTGCCATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTCTCGTCGTATCCGTCACCCCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGAACTGGAACGATGGGCCGAAGGTtg  >  2:284954/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCTGGCAAACCTGAACGGTTTTGATCCAGCAAAAGATATGGTGAAACCGGAAGAGATGGTGGTACTGGATCGCTGGGCCGTAGGTTG  >  NC_000913/23988‑24462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑