Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,133	G→A	K581K (AAG→AAA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,133	0	G	A	100.0%	179.2 / NA	57	K581K (AAG→AAA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/34);  total (23/34)

GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCT  >  NC_000913/23888‑24378                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      gttgaagttaacggCATCCAGGCGGGGTGGGATCTCGATGAGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGGATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGCAGAAgggca                                                                                                                                                                                                                                                   >  2:405826/13‑248 (MQ=255)       gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                                                                                                                                                                                                            <  1:375991/245‑1 (MQ=255)          aacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGt                                                                                                                                                                                                                                          >  1:326704/4‑250 (MQ=255)             ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCg                                                                                                                                                                                                                                      <  1:426267/251‑1 (MQ=255)              gCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGt                                                                                                                                                                                                                                     <  1:286426/251‑1 (MQ=255)                       cGTGGTGGGATCGCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTGCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTg                                                                                                                                                                                                                            <  1:293340/251‑1 (MQ=255)                             gggagcccGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                                                                                                                                                                                                      <  2:158392/244‑1 (MQ=255)                               gATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCAACCGCGATGAAAGGAAAAGCGCCGTATCGTCAGGTACTTACCAACgg                                                                                                                                                                                                                    >  2:368565/1‑251 (MQ=255)                                     gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtttcc                                                                                                                                                                                                              >  2:51790/1‑249 (MQ=255)                                           aaaGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGa                                                                                                                                                                                                        <  1:370176/251‑1 (MQ=255)                                            aaGAGATCCTCGGCGACGAAGCTGCCGAGTATGTGAAAGTGCCGGATTCTCTC‑GACGTATGGTTTGACTCCGGACCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATTTATCTGGAAGGTTCTGACCAGCGCCGTGGTTGGTTCATGGCTTTCTTGATGATCGCCTCCGTGATGAAAGGCAAGGCGCCGTAGCGTCAGGATCTGATCCAGGGCTCTACCGTGGAt                                                                                                                                                                                                       <  2:211957/251‑1 (MQ=255)                                             agagATCCTCGGCGACGAAGCTGACCAGTAAGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCTTCAGGTACTGATCCACGGCTTTACCGTGGATg                                                                                                                                                                                                      <  2:289218/251‑1 (MQ=255)                                               agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGt                                                                                                                                                                                                    <  1:481228/251‑1 (MQ=255)                                                          gacgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGAGGATGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                                                                          >  2:384546/1‑250 (MQ=255)                                                           acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                                                                          >  1:259883/1‑249 (MQ=255)                                                              aaGCTGACCAGTATGTGAAAGTGCCGGATAC‑TCGCGACGGATGGTTTGACTCCGGATCTACCGACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTATCGGCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATg                                                                                                                                                                                     <  2:56897/251‑1 (MQ=255)                                                                gCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc                                                                                                                                                                                   <  1:427841/251‑1 (MQ=255)                                                                   gaccAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTTACCCACGGTTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTaa                                                                                                                                                                                <  1:191976/248‑1 (MQ=255)                                                                      cAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTTTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCTGCAAGATGTCTAAATc                                                                                                                                                                             <  1:409289/251‑1 (MQ=255)                                                                       aGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATcc                                                                                                                                                                            <  2:358757/251‑1 (MQ=255)                                                                            gTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAGGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAGCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGTCCCACGGCTTTACCGTGGATGGTTAGGGCCGCAAGATGTCTAAATCCATCgg                                                                                                                                                                       <  1:444009/251‑1 (MQ=255)                                                                              gAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGcc                                                                                                                                                                     >  2:253612/1‑250 (MQ=255)                                                                                                      tATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGAAATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACAACGTTTCGCCGCAGGAtgtg                                                                                                                                              >  1:55187/1‑251 (MQ=255)                                                                                                       ttGGTTTGACTCCGGATCTACCCCCTCTTCTGTTGTTGACTTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAAGGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgtg                                                                                                                                              <  2:81624/249‑1 (MQ=255)                                                                                                       aTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtga                                                                                                                                             <  2:85138/251‑1 (MQ=255)                                                                                                            tgtaCTCCGTATCGACCCACTCTTCTGTTGTTGATGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGGTCAGACCAGCACCGTGGTTTGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACTGCTTGACCGTGGATGTGCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGaa                                                                                                                                         <  1:30227/247‑1 (MQ=255)                                                                                                              gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                                                                       <  1:420372/250‑1 (MQ=255)                                                                                                               aCTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaaa                                                                                                                                     <  1:242477/251‑1 (MQ=255)                                                                                                                 tCCGGATCGTCCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAATGTTCTGACCAGCACCGGGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCTCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGTATTGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTTCGCCGTCGGATGTGATGATCAAAc                                                                                                                                    <  2:324802/250‑1 (MQ=255)                                                                                                                  ccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAGTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg                                                                                                                                  <  2:391510/251‑1 (MQ=255)                                                                                                                              aCTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATAtt                                                                                                                      <  1:415489/251‑1 (MQ=255)                                                                                                                                  ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCCGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGGTGTGATGAACaca                                                                                                                                     >  2:83466/1‑230 (MQ=255)                                                                                                                                  ttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaaa                                                                                                                                     <  1:83466/232‑1 (MQ=255)                                                                                                                                           tgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCATCTCCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCATGGCCGCAAGATTTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGTCGCGGATATTCTGCGTCTgtgg                                                                                                          <  1:453039/250‑1 (MQ=255)                                                                                                                                                    tCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGGGGATGGTTCATGTCTTACCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCATTACCGTGGATGGACAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAAAAACTGGGCGAGGATAATCTGCGTCTGGGGGGGGAATCa                                                                                                 >  2:140976/1‑250 (MQ=255)                                                                                                                                                               ccGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCCCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACAcc                                                                                     <  2:326704/251‑1 (MQ=255)                                                                                                                                                                        cAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATg                                                                            >  1:27385/1‑251 (MQ=255)                                                                                                                                                                         aGCGGACATGTAGCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATgg                                                                           <  2:119022/251‑1 (MQ=255)                                                                                                                                                                            ggACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCCCGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAACTCCATCGGCACCACCGTTTCCCCGCAGGATGTGATGAACAAACTGGGCGCGGCTATTCTGCGTTTGTGGGTGGCATCAACAGACTACACCGGTGAAATGGCCg                                                                        >  2:238099/1‑251 (MQ=255)                                                                                                                                                                                           aGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCCTCAACCGACTACACCGGTGAAATGGCCGTTTCTGACTAGATcc                                                         >  1:259952/1‑251 (MQ=255)                                                                                                                                                                                                  gACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACg                                                  <  1:131690/251‑1 (MQ=255)                                                                                                                                                                                                   aCCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTAACGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGt                                                 >  1:279165/1‑251 (MQ=255)                                                                                                                                                                                                    ccagcaccgtggttgggtcttGTCTTCCCTAATGATCTCCACCGCGATGAAAGTCAAAGCGCCGTATCGTCAGGTATTGACCCACGGCTTTACCTTGGATGGTCAGGGCCGCAAGATGTCTACATCCATCGGCAACACCGGTTCGCCGCAGGAGGTGATGAACAATCTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACTCCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGtg                                                <  1:67218/231‑1 (MQ=255)                                                                                                                                                                                                         aacgcgGTTGGTTCATGTCTTCCCTAATGATCTCCACAGCGATGTAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGAATGTCAGGGCCGCAAGCTGTCTAAATCCATCGGAAAAACCGTTTCGCCGCAGGTAGTGCTGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATAAACCGACTACACCGGTGACATGACCGTTTCTggagcgatcttgacacaggtggcc                                           >  2:377586/3‑228 (MQ=255)                                                                                                                                                                                                          ccgtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCg                                          >  2:91736/8‑251 (MQ=255)                                                                                                                                                                                                            gtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                        <  1:470000/246‑2 (MQ=255)                                                                                                                                                                                                            gtggttGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGGATCGTCAGGGACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACTCCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATGCTTCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGac                                        <  2:46217/246‑2 (MQ=255)                                                                                                                                                                                                                ttgggtCATGTCTTCACTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACAACGTTTCGCCGCAGGATGTGATGAAAAAAATGGACGCGGATATTCTGCGGCTGTGGGGGGCATCAACCGACTACACCGGTGAAATGCCCGTTTCTGACGAGTTCCTGCAACGAGCAGCCGACAGcg                                    >  2:199089/6‑250 (MQ=255)                                                                                                                                                                                                                  ggTTCATGTCTTCCCTAATGATCTCCACCGCGAGGAAAGGCAAAGCGTCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACCCCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGGGAAATGGCCGTTTCTGACGAGATCCTGAAACGAGCTGCCGACAGCTAt                                  <  1:115812/251‑1 (MQ=255)                                                                                                                                                                                                                      cATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTAtcgtc                              <  1:154678/251‑1 (MQ=255)                                                                                                                                                                                                                         gTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTATGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGCAATGGCCGTTTCTGACGAGATCCTGAAACGTACTGCCGACAGCTATcgtcgt                            >  1:366642/1‑250 (MQ=255)                                                                                                                                                                                                                         gTCTGCCCTAATGATCTCCCCCGCGATGAAAGGCAAAGCTCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCATGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATcgtcgt                            <  1:333189/250‑1 (MQ=255)                                                                                                                                                                                                                                   aTGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAacac                 >  1:485384/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACAcc                >  2:134318/1‑251 (MQ=255)                                                                                                                                                                                                                                        ctcCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGTAACACcgcgc            >  1:28969/1‑251 (MQ=255)                                                                                                                                                                                                                                        ctcCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGCATCAACCGACTACCCCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTACCCGTAACACcgcgc            >  2:193783/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgcgATGAAAGGCAAAGGGCCGTATCGTCAGGTACTGAACCACGGCTTTACCGGGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGCAACGTGATGCCGACAGCTAACGTCGTAACCGTAACACCGCGCGCTTcc      >  2:161035/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aTGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGACAGCTATCGTCGTATCCGCAACACCGCGTGCTTCccgct  >  1:278413/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      GTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGCCGATAGCTATCGTCGTATCCGTAACACCGCGCGCTTCCTGCT  >  NC_000913/23888‑24378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑