| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,116 | T→C | Y594H (TAT→CAT) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,116 | 0 | T | C | 98.5% | 188.0 / ‑5.6 | 66 | Y594H (TAT→CAT) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/34); minor base A (1/0); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 | |||||||||||
TTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTT > NC_000913/1883‑2359 | taaaTCTGGAAAACTGACAGGCAGAACTTGCGCAAGCCAAATAGACGTTTAATCTCAGGCGCTTAATTCGCCTCGGGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATAAATATGCCGACTTCTTG‑TGCGAAGGTTTCCCCGATTTCAAGCCGAACACAAAGGCCAACAACTCGTCGCTGGATTTCTACCATCTGTTGCGTAATGAGGCGGaaacaac > 2:320511/3‑246 (MQ=255) ggAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTa < 1:82318/251‑1 (MQ=255) ggAAAACGGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCgcggcg < 1:85655/249‑1 (MQ=255) acgggCAGGAAGAACTGGCGCAAGCAAAAGAGCCGTTTAATCTCTGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTc < 2:429785/248‑1 (MQ=255) cAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCctctt > 1:70853/1‑250 (MQ=255) gggcccAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAAATCGCCGCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTTGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAAc < 2:48300/246‑1 (MQ=255) aaGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGCAGGTTTCCCCGTTGGCACGCCGAACAAAAAAGCCAACACCTCGTCGATGGATTACTACCATCTGTTGAGTCATGCGGCGGAAAAATCGCGGGGCAAATTCCTCCATTAC‑ACCCACGTTggg > 2:347033/1‑251 (MQ=255) ccAAAGAGCCGTTTAATCTCGGGCGCTTACTTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGAATGCACTTCCAGCCAGGCAGTGGCGGATTAACATGCCAACTTCTTG‑AGCGGAGGTTTCCACGTTGTCAAGACGCAAAAAAAGGACAACACCTCGACGCTGGATTCATACCATCTGTTGCGTCATGCGGAGGAAAACTCGCGGCGTAAATTCCTCTATGAC‑AACAACGTTGGGGCt > 1:24689/1‑251 (MQ=255) cAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTg > 1:473497/1‑251 (MQ=255) aaatagCCGTTTAATCTCGGGCGCGTAAGTCGCCTCGTGGAAGAAGATCATCGGCTGAGACCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTGCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGGCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTgg < 2:114108/247‑1 (MQ=255) aaGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGGTGTCACCCCGAACAAAAAGGCCAACTCCTCGTCGATGAGTAACTACCCCCTGTTGCGTCCTTCGGCGGAAAAATCGCGGCGGAAATTCCTCTCTTAC‑ACCATCGTTgggggtagt > 1:147889/1‑246 (MQ=255) gTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGTAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCgg < 1:232701/251‑1 (MQ=255) tttAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGt > 1:339846/1‑251 (MQ=255) agggCGCTTAATCTGCCTCGTGAAAGAATATCATCGGCTGAACCCGGTCATTGTTGACTTCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑CCCAACGTTGGGGCTGGATTACCGGTTATTga < 2:37748/248‑1 (MQ=255) cgcTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTTCCGGTTATTGAGAACCt > 1:492039/1‑251 (MQ=255) gcTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTg < 2:403446/251‑1 (MQ=255) cTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGGAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGc > 1:73613/1‑251 (MQ=255) cGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATc < 1:3115/250‑1 (MQ=255) cGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCGCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa < 1:87469/251‑1 (MQ=255) gTGAAAGAATATCCTCTGCTGAACCCGGCCATTGTTGACTGCACTTCCAGCCAGGCGGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCa > 1:412795/1‑250 (MQ=255) aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAcc < 2:274471/232‑1 (MQ=255) aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAcc > 1:274471/1‑232 (MQ=255) aaaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGc > 1:90775/1‑251 (MQ=255) aaGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATTTGCCGACTTCTTT‑CGCGAAGGGTTCCACGTTGTCACGCCGACCAAAAAGGCCCACACCTCGGCGATGGATTAATACCATCTGTTGCGCCCAGAGGCGGAAAAAACGCGGCGGACATTCCTCTATTAC‑CACACAGTTGGGGCTTGATTACCGGTTATTGAGAACCTTCAAAAACTGCTTAATGct > 2:187308/1‑250 (MQ=255) gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaaagctgg > 1:28912/1‑245 (MQ=255) gAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGACAACACCTCGTCGATGGATTACTACCATCTGGTGCGTCATGCGGCGGAAAAATCGCGGCGTAAAATCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGATCATTCAAAATCTGCTCAATgatgg > 1:12012/1‑247 (MQ=255) catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCTCGGCGTAAATTCCTCTATGAC‑ACCCACGTTGGGGCTGGCTTACCGGTTATTGGGAACCTGCAAAACTTGCTCAATGcttggtgtg > 2:377939/1‑242 (MQ=255) catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatg < 1:136969/250‑1 (MQ=255) cccGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGt < 1:61987/251‑1 (MQ=255) aTTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCgg < 2:90775/251‑1 (MQ=255) gACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttct < 1:377939/251‑1 (MQ=255) gCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGAGTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttca < 2:278264/251‑2 (MQ=255) cACTTCCAGCCAGGCAGTGGGGGATCAATATGCCGACTTCTTG‑CGCGAAGGGTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttcag < 2:73613/251‑3 (MQ=255) aCTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGCAAAATCGCGGCGTAAATTCCTCTATGACCACCA‑CGTTGGGGCTGGATTACCGGATATTGTGAACCTGCAAAATCTGCTAAATGCTGGTGATGAATTGATTTAGTTCTCCGCCAttctttcagg > 2:394727/1‑248 (MQ=255) tCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGt < 1:197270/249‑1 (MQ=255) agccagGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtgat < 2:176907/221‑1 (MQ=255) agccagGCAGTGGCGGATCAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtgat > 1:176907/1‑221 (MQ=255) ccaagCAGTGGCGGATCAATATGCCGAATTCGTG‑CGCGAAGGTGTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTGCCATCTGTTGCGGCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAGGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCCCAATGCTTGGGATGAATTGATGAAGTTCTCCGTCATTCTTTCTGGCTCGCttt < 2:329950/247‑1 (MQ=255) gTGGCGGTGCAATATGCCGTCTTCTTGACG‑GAAGGTTTCCCAGTTGTCACGCCTAACAAAAAGGCCAGCACCTCGGCGATGGAGTACTACCATCTTTTGCTTCATGCTGCGGAAAAGTCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGTGGCTGTATTACCGGTTATGTAGATCCTGCAAATTCTGCTCTATGCTTGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGCCTCGCTTTCTTatat < 2:444020/251‑1 (MQ=255) ggCGGATCAATAGTCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATGCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATc < 2:422611/250‑1 (MQ=255) gCGGAACAATATGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCtt < 2:326550/251‑1 (MQ=255) atatGCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGCATTGCTGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGt > 2:182059/1‑251 (MQ=255) gCCGACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAGCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGa < 1:300740/251‑1 (MQ=255) gCCGACTGCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGa < 1:248510/251‑1 (MQ=255) gACTTCTTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg > 1:404375/1‑250 (MQ=255) tG‑CGCGAAGGTTTCAACGTTGTCACGCCGAACAAAAAGGACAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATACCTCTATGAC‑ACCAACGTTGGGACTAGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCAt < 2:473497/250‑1 (MQ=255) gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGTTGAAGTTCTCCGGCTTTCTTTCAGGTTCGCTTTCTTATATCTTCGGCATGTTAGACGAAGGCTTGAGttt > 2:423138/1‑251 (MQ=255) gAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt > 2:223725/1‑251 (MQ=255) aaGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTACGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTtc > 1:328600/1‑251 (MQ=255) tCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTTTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCg > 2:238844/1‑249 (MQ=255) gTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTTGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGGTAGACGAAGGCATGAGTTTCTCCGAGGCGActa < 1:438963/250‑3 (MQ=255) aCGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATCTCTTCGGCAAGTTAGACGAAGGCATGAGTTTATCCGAGGCGActactct > 2:246558/1‑245 (MQ=255) cGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGActactctg < 2:404375/251‑8 (MQ=255) cccGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCAGGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGAGGAAGTTCTTCGGCATTCTTTCAGGTTCGCTTTCGTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTGCTCCGAGGCGACTACTCTgg < 2:499677/250‑1 (MQ=255) aaCAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGcgcg > 2:291079/1‑251 (MQ=255) cAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACAGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGGAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCggg > 2:59206/1‑251 (MQ=255) aaaaGTCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCATGTTCGCTTTCTTATATCTTCGGCa < 1:279767/201‑1 (MQ=255) aaaaGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCa > 2:279767/1‑201 (MQ=255) ggccacagccTCGTCGATGGAGTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATTAC‑ACCATCGTGGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATTTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCGTTTAGGTTCGCTTTCTTATATCTTCGGTAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATgg < 1:300402/243‑1 (MQ=255) aaCACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAACCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTta < 1:441224/251‑1 (MQ=255) acCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGCTGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATAc > 1:70531/1‑251 (MQ=255) aTGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCgg > 1:102928/1‑250 (MQ=255) ccATCTGTTGCGTCAGGCGGCGGAAAAATCGCGGCGTAAATTCCGCTATGAC‑TCCAACGTGGGTGCTGGATTACCGGTTATTGAGATCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGGTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatg < 2:73504/251‑1 (MQ=255) tgTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCtt > 1:168246/2‑251 (MQ=255) gTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAAGCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAGATGATCtt < 2:70853/250‑1 (MQ=255) gTTGCGTCATGCGGCGGAAAAATAGCGGCGTAACTTCCTCTATGAC‑ACCACCGTTGGGGCTGGATTACCGGATATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGCCGCAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATCCCGAACCGGATCCGCGAGATGATCttt > 2:176078/1‑251 (MQ=255) | TTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTG‑CGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGAC‑ACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGATCTTT > NC_000913/1883‑2359 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |