Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,956	T→C	D522D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,956	0	T	C	95.2%	239.2 / 1.5	83	D522D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (30/49);  total (32/51)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

GCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTC  >  NC_000913/23709‑24201                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gCGTGCAGTGGATCCCGGACTGGGGTCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAg                                                                                                                                                                                                                                                     <  1:112495/251‑1 (MQ=255)     gCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCTAAATAGATCCTCGGCGACGAAGCTGACCAGTatg                                                                                                                                                                                                                                                 <  1:145560/251‑3 (MQ=255)          ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                                                                                                                                                                            <  1:355721/251‑1 (MQ=255)            aTCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGACATGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAACTGACCAGTATGTGTAAgg                                                                                                                                                                                                                                          >  2:370176/1‑250 (MQ=255)               ccGGTCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCTGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACGCGGAAGAGCTGCATCCGCGTACACGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                                                                                       <  2:128615/251‑1 (MQ=255)               ccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                                                                                       <  1:274520/251‑1 (MQ=255)                    cgggggCCCGGGGCGTATCGAGTCGATGGTTGCTAACCGGCCTGACATGG‑GTATATCCCGTCAGCGCACCTGGGGCGTACCGATGACACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATACAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                                                                                                                  <  2:308383/249‑3 (MQ=255)                     tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                                                                                                                                                                                 >  1:85138/1‑248 (MQ=255)                     tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTCTGTGAAAGGGCCGGatac                                                                                                                                                                                                                                 >  2:242477/1‑248 (MQ=255)                      ggtgccgggCGCTTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGGCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAATCGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                                                                                                                  <  2:182285/242‑3 (MQ=255)                      ggggcagagggcgTATCGAGTCGAGGGTTGCTAACCGTCCTGAC‑TGTTGTATCTCCCGTCAGCGCTCCGGGGGCGTACCGATGTCACTGGTCGTGCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCACCCAGGCGTGGTGGGATCTCGATGCGATAGAGATCCTCGGCGACGAAGCTGCCCATTATGTGAAAGTGCCGGatact                                                                                                                                                                                                                                <  2:419814/241‑5 (MQ=255)                            aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                                                                                          <  1:491466/251‑11 (MQ=255)                            aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                                                                                          <  2:461034/251‑11 (MQ=255)                                    aTCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggtt                                                                                                                                                                                                                  <  1:169492/251‑19 (MQ=255)                                        gggcGATGGTTGCGAACCGGCCTGAC‑TGGGGTAACTCCAGAAATCGCACCAGGGGCGGACCGATGTCACTGTACGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATTCAGTCGTGGTGGGATCTCGATACGAAAGAGAGCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTggc                                                                                                                                                                                                             <  2:249766/248‑3 (MQ=255)                                        aGTCGATGGGTGCTAACCGTCCTGAC‑TGGCGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtagggtttgtc                                                                                                                                                                                                              >  2:293340/1‑229 (MQ=255)                                             aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                        <  2:435776/251‑1 (MQ=255)                                             aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCgg                                                                                                                                                                                                        <  1:470153/251‑1 (MQ=255)                                                     tAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGACACTCTC‑GCCGAATGGTTTGCCTCCGGATCTcccc                                                                                                                                                                                                >  2:154678/1‑251 (MQ=255)                                                      aaCCGGCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTGCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAGC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACGCTC‑GACGTATGGTTTGACTCCGGATCTACCCa                                                                                                                                                                                               <  2:411794/251‑1 (MQ=255)                                                       aCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                                                                                              >  1:238582/1‑251 (MQ=255)                                                                 c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGACAGTGCCGGATACTCTC‑GCCGTATGGTTTGACTCCGGATCTACCCCCTCTTCtgttg                                                                                                                                                                                    >  2:369942/1‑251 (MQ=255)                                                                 c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCAGTATGTGAAAGTGCCGGATCCTCTC‑GACGTATGGTTTGACTACGGATCTACCCACTCTTCtgttg                                                                                                                                                                                    >  2:420372/1‑251 (MQ=255)                                                                    ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCGTCtgttg                                                                                                                                                                                    <  1:226231/249‑1 (MQ=255)                                                                        tATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                                                                                                                                                                              >  1:306020/1‑251 (MQ=255)                                                                           ctcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGc                                                                                                                                                                           >  1:46217/1‑251 (MQ=255)                                                                             cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt                                                                                                                                                                         >  1:174440/1‑251 (MQ=255)                                                                                gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                      <  2:83428/251‑1 (MQ=255)                                                                                   aGCGCAACTGGCGCGTACCAGTGTCCCTGTTCGTGCACAAAGGCACGGCAGAGCTGGATTCGAGTACCCTCGAACTGAGGGAAGAAGGTGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGaa                                                                                                                                                                   <  2:118366/251‑1 (MQ=255)                                                                                    gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt                                                                                                                                                                  <  1:355593/251‑1 (MQ=255)                                                                                      gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                                                                                                                                <  1:231656/251‑1 (MQ=255)                                                                                       ccccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACAAGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                                                                                                                               <  2:225673/249‑1 (MQ=255)                                                                                       cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTg                                                                                                                                                               >  2:415489/1‑251 (MQ=255)                                                                                         ccTGGGGCGTACCGATGGCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc                                                                                                                                                             <  1:360500/251‑1 (MQ=255)                                                                                                cgtacctaggtcactgtgccggCACAAAGACACGGAAGAGCTGCATCCTCGGACCCGCGAACTGATGGAAGAAGTGGCAAAGCGCGTTGAAGT‑TATCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACTAATCTGACCAGTAGGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTGGATTCCGGATCTTCCCACTTTTCTTTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                      <  2:463813/230‑1 (MQ=255)                                                                                                 gtACCGATGTCACTGCTCGGGCTCAAGGACTCTGTAGTGTGGCATCCGCGTACACCCGAACTGATGGGAGAAGTGGCAAAGCGCGTTGAAGTTAAC‑TGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGAGCCTGATACTCTC‑TTCGTAGGGTGCGACTCCGGAGCTACCCACTCGTCTGTTGGTGACGTGCGTCCGGAATTTGCCggctacgc                                                                                                                                                     <  2:458354/251‑7 (MQ=255)                                                                                                     cGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                                                                                                                 >  1:119022/1‑251 (MQ=255)                                                                                                         gTCACTGTTAGAGCACAAAGAAACGGAAGAGATGCCTCCGCGTACCCTCGACCTGCTGGAAGAAGTGGCAAACCGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GTCGTCTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGAGTCCGGAATTTGCCGGTCACGCAGCGGAcc                                                                                                                                             >  2:500538/1‑250 (MQ=255)                                                                                                          tCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                                                                                            >  1:213132/1‑251 (MQ=255)                                                                                                           cACTGTTCGTGCACAAATGCACCGAAGATCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                                                                                            <  1:30699/250‑1 (MQ=255)                                                                                                           cACTGTTCGTGCACAAAGACTCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGTTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATg                                                                                                                                           <  2:177918/251‑1 (MQ=255)                                                                                                               ggtCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCGTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTa                                                                                                                                         <  2:137850/247‑1 (MQ=255)                                                                                                                 tCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                                                                                                                                     >  1:289218/1‑251 (MQ=255)                                                                                                                        caAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGCCCGGAATTTGCCGGTAACGCAGCGGACATGTATCTGGAAGGtt                                                                                                                              <  1:441694/251‑1 (MQ=255)                                                                                                                         aaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCCTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAACGGCCATGTATCTGGAAGGTTc                                                                                                                             >  2:131690/1‑251 (MQ=255)                                                                                                                                    agagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GTCGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGCATTTGCCGGTCACGCAGCGGCCATGTATCTGGTAGGTTCTGAcccgcacc                                                                                                                  >  2:150548/1‑245 (MQ=255)                                                                                                                                     gagCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcg                                                                                                                 <  1:23998/251‑1 (MQ=255)                                                                                                                                          gCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt                                                                                                            <  1:262738/251‑6 (MQ=255)                                                                                                                                            aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                                                                                          <  1:385456/251‑8 (MQ=255)                                                                                                                                                   tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCCGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATgg                                                                                                   >  2:11691/1‑250 (MQ=255)                                                                                                                                                    aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                   <  1:371679/250‑1 (MQ=255)                                                                                                                                                    aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc                                                                                                  >  1:7771/1‑251 (MQ=255)                                                                                                                                                     cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc                                                                                                  <  1:502111/250‑1 (MQ=255)                                                                                                                                                     cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCt                                                                                                 >  1:366720/1‑251 (MQ=255)                                                                                                                                                       cgcgAACTGATGGAAGAAGGGGCCAAACGCGTTGCAGGTTAC‑GGCATCCAGGCGTGGTGGGATCTATATGCGAAAGAGATCCTCGGCGAAGAAGCTGACAAGTATGTGAAATTGCCGGATACTCTC‑GACGTATGGTTTGCCTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCTGCACCGTGGTTGGTTCATGTCtgc                                                                                               <  2:373539/248‑3 (MQ=255)                                                                                                                                                         cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                                                                                             >  1:8109/2‑251 (MQ=255)                                                                                                                                                          gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTAccc                                                                                                                                                                                                <  1:470990/151‑1 (MQ=255)                                                                                                                                                          gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTAcac                                                                                                                                                                                                >  2:470990/1‑149 (MQ=255)                                                                                                                                                                     agaagTGGCAAAACGCGTTGCAGTTAAC‑GGCATCCAGGCGTAGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGATTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACAGTGGTTGGTTGATGTATTCCCTAATGATCTccc                                                                                 >  2:333189/1‑250 (MQ=255)                                                                                                                                                                      gaagTGGCAAAACGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAc                                                                                <  1:73381/251‑1 (MQ=255)                                                                                                                                                                               aaaCGCGTTGAAGTTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa                                                                       <  1:170297/251‑1 (MQ=255)                                                                                                                                                                                   cgcggtgaaattaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAATATATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCGTCTTTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTGCATGTCTTCCCTAATGATCTCCACCGCGATGaaagg                                                                    <  2:420144/236‑4 (MQ=255)                                                                                                                                                                                      ttGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGt                                                                                                                               >  1:144464/1‑188 (MQ=255)                                                                                                                                                                                      ttGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGt                                                                                                                               <  2:144464/188‑1 (MQ=255)                                                                                                                                                                                      gttgaagttaacggCATCCAGGCGGGGTGGGATCTCGATGAGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGGATCTGGAAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGCAGAAgggca                                                                  >  2:405826/13‑248 (MQ=255)                                                                                                                                                                                            gttaacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGcc                                                           <  1:375991/245‑1 (MQ=255)                                                                                                                                                                                               aacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGt                                                         >  1:326704/4‑250 (MQ=255)                                                                                                                                                                                                  ggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCg                                                     <  1:426267/251‑1 (MQ=255)                                                                                                                                                                                                   gCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGt                                                    <  1:286426/251‑1 (MQ=255)                                                                                                                                                                                                            cGTGGTGGGATCGCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTGCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTg                                           <  1:293340/251‑1 (MQ=255)                                                                                                                                                                                                                  gggagcccGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCAc                                     <  2:158392/244‑1 (MQ=255)                                                                                                                                                                                                                    gATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCAACCGCGATGAAAGGAAAAGCGCCGTATCGTCAGGTACTTACCAACgg                                   >  2:368565/1‑251 (MQ=255)                                                                                                                                                                                                                          gATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTc                                                                                                                             <  1:201754/155‑1 (MQ=255)                                                                                                                                                                                                                          gATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCtttcc                             >  2:51790/1‑249 (MQ=255)                                                                                                                                                                                                                          gATGCGAAAGAGATCCTCGGCGAAGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTc                                                                                                                             >  2:201754/1‑155 (MQ=255)                                                                                                                                                                                                                                aaaGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGa                       <  1:370176/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaGAGATCCTCGGCGACGAAGCTGCCGAGTATGTGAAAGTGCCGGATTCTCTC‑GACGTATGGTTTGACTCCGGACCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATTTATCTGGAAGGTTCTGACCAGCGCCGTGGTTGGTTCATGGCTTTCTTGATGATCGCCTCCGTGATGAAAGGCAAGGCGCCGTAGCGTCAGGATCTGATCCAGGGCTCTACCGTGGAt                      <  2:211957/251‑1 (MQ=255)                                                                                                                                                                                                                                  agagATCCTCGGCGACGAAGCTGACCAGTAAGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCTTCAGGTACTGATCCACGGCTTTACCGTGGATg                     <  2:289218/251‑1 (MQ=255)                                                                                                                                                                                                                                    agaTCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGt                   <  1:481228/251‑1 (MQ=255)                                                                                                                                                                                                                                               gacgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGAGGATGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGAACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa         >  2:384546/1‑250 (MQ=255)                                                                                                                                                                                                                                                acgaAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa         >  1:259883/1‑249 (MQ=255)                                                                                                                                                                                                                                                   aaGCTGACCAGTATGTGAAAGTGCCGGATAC‑TCGCGACGGATGGTTTGACTCCGGATCTACCGACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAATGATCTCCACCGCTATGAAAGGCAAAGCGCCGTATCGGCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATg    <  2:56897/251‑1 (MQ=255)                                                                                                                                                                                                                                                     gCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTc  <  1:427841/251‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTC  >  NC_000913/23709‑24201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑