Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,899	T→C	D503D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,899	0	T	C	94.7%	228.5 / ‑1.9	76	D503N (GAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (28/44);  total (29/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAA  >  NC_000913/23657‑24132                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           gtcagcgaggaacagaaaggacctcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGGGGATCCCGGACTTGGGCCATGCGCGTATCGAGCCGATGGTTGATAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTTCCGATGTCACGGTTCGTGCACAATG‑ACGCGGAAGAGCTGCATGCGCGTACCCTCGATCTGATGGAAGAAGTTCCAAAACGCGTTGAAG‑TTAAT‑GGCATCCa                                                                                                                                                                                                                                   <  2:483235/227‑1 (MQ=255)   cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAgg                                                                                                                                                                                                                                 <  1:202902/251‑1 (MQ=255)              agacaGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGAt                                                                                                                                                                                                                      <  1:395486/247‑1 (MQ=255)                      cTGCGTGCACAGCCACTGAAAGAGCTCGAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTGACTCCCGTCAGCGACCCTGGGGCGTACCGATGTCACTGTGCGTGAACAAAGGACA‑GGAAGAGCAGCATCCGCGTACCCTCATGCCGATGGAAGCCGAGGCAAAACGCGGTGAAGGTC‑AC‑GGCATCCACGCCGGGTGGGAGCTCGATGc                                                                                                                                                                                                              >  2:298551/1‑251 (MQ=255)                             ggcAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACATGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                                                                                                       <  1:23250/250‑1 (MQ=255)                             cgcAGTCACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGGCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAagag                                                                                                                                                                                                       <  2:125094/251‑1 (MQ=255)                                  tCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGGTGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATATCGATGCGAAAGAGATcat                                                                                                                                                                                                  >  2:409289/1‑249 (MQ=255)                                   cACTGAAAGAGAGCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTc                                                                                                                                                                                                 <  1:79061/251‑1 (MQ=255)                                      tGAAAGAGGTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCATCCTGAC‑TGGTGTCTCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAGG‑ACACGCACGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGCAGTGGCAACTCCCGTTGAGG‑AGAAC‑GGCATCCAGGCGTGGTGGGaccccggtgagaacgagatcaacggg                                                                                                                                                                                              >  2:444009/1‑226 (MQ=255)                                              aTCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGc                                                                                                                                                                                      <  1:49613/251‑1 (MQ=255)                                                   aGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCTAAAGAGATCCTCGGCGACGAAGCTGacc                                                                                                                                                                                 <  1:433753/251‑3 (MQ=255)                                                     gCGTGCAGTGGATCCCGGACTGGGGTCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAg                                                                                                                                                                               <  1:112495/251‑1 (MQ=255)                                                         gCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCTAAATAGATCCTCGGCGACGAAGCTGACCAGTatg                                                                                                                                                                           <  1:145560/251‑3 (MQ=255)                                                              ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGaaa                                                                                                                                                                      <  1:355721/251‑1 (MQ=255)                                                                aTCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGACATGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCCGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAACTGACCAGTATGTGTAAgg                                                                                                                                                                    >  2:370176/1‑250 (MQ=255)                                                                   ccGGTCTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCTGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACGCGGAAGAGCTGCATCCGCGTACACGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                 <  2:128615/251‑1 (MQ=255)                                                                   ccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGcc                                                                                                                                                                 <  1:274520/251‑1 (MQ=255)                                                                        cgggggCCCGGGGCGTATCGAGTCGATGGTTGCTAACCGGCCTGACATGG‑GTATATCCCGTCAGCGCACCTGGGGCGTACCGATGACACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATACAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                                            <  2:308383/249‑3 (MQ=255)                                                                         tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                                                                                                           >  1:85138/1‑248 (MQ=255)                                                                         tGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGCCGAAGCTGACCAGTCTGTGAAAGGGCCGGatac                                                                                                                                                           >  2:242477/1‑248 (MQ=255)                                                                          ggtgccgggCGCTTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTTGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAATCGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGata                                                                                                                                                            <  2:182285/242‑3 (MQ=255)                                                                          ggggcagagggcgTATCGAGTCGAGGGTTGCTAACCGTCCTGAC‑TGTTGTATCTCCCGTCAGCGCTCCGGGGGCGTACCGATGTCACTGGTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCACCCAGGCGTGGTGGGATCTCGATGCGATAGAGATCCTCGGCGACGAAGCTGCCCATTATGTGAAAGTGCCGGatact                                                                                                                                                          <  2:419814/241‑5 (MQ=255)                                                                                aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                    <  1:491466/251‑11 (MQ=255)                                                                                aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                    <  2:461034/251‑11 (MQ=255)                                                                                        aTCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatggtt                                                                                                                                            <  1:169492/251‑19 (MQ=255)                                                                                            gggcGATGGTTGCGAACCGGCCTGAC‑TGGGGTAACTCCAGAAATCGCACCAGGGGCGGACCGATGTCACTGTACGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATTCAGTCGTGGTGGGATCTCGATACGAAAGAGAGCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTggc                                                                                                                                        <  2:249766/248‑3 (MQ=255)                                                                                            aGTCGATGGGTGCTAACCGTCCTGAC‑TGGCGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtagggtttgtc                                                                                                                                        >  2:293340/1‑229 (MQ=255)                                                                                             gTCGATGGTTGCTACCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAg                                                                                                                                                                                                                                  >  2:306909/1‑160 (MQ=255)                                                                                             gTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAg                                                                                                                                                                                                                                  <  1:306909/160‑1 (MQ=255)                                                                                                 aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                                                                                                                   <  2:435776/251‑1 (MQ=255)                                                                                                 aTGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCgg                                                                                                                                   <  1:470153/251‑1 (MQ=255)                                                                                                         tAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGGATGTGAAAGTGCCGGACACTCTCGCCGAATGGTTTGCCTCCGGATCTcccc                                                                                                                           >  2:154678/1‑251 (MQ=255)                                                                                                          aaCCGGCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTGCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAGC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACGCTCGACGTATGGTTTGACTCCGGATCTACCCa                                                                                                                          <  2:411794/251‑1 (MQ=255)                                                                                                           aCCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                         >  1:238582/1‑251 (MQ=255)                                                                                                                     c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGCTGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGCGATCCTCGGCGACGAAGCTGACCAGTATGTGACAGTGCCGGATACTCTCGCCGTATGGTTTGACTCCGGATCTACCCCCTCTTCtgttg                                                                                                               >  2:369942/1‑251 (MQ=255)                                                                                                                     c‑TGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCAGTATGTGAAAGTGCCGGATCCTCTCGACGTATGGTTTGACTACGGATCTACCCACTCTTCtgttg                                                                                                               >  2:420372/1‑251 (MQ=255)                                                                                                                        ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCGTCtgttg                                                                                                               <  1:226231/249‑1 (MQ=255)                                                                                                                            tATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACg                                                                                                         >  1:306020/1‑251 (MQ=255)                                                                                                                               ctcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGc                                                                                                      >  1:46217/1‑251 (MQ=255)                                                                                                                                 cccGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGt                                                                                                    >  1:174440/1‑251 (MQ=255)                                                                                                                                    gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                 <  2:83428/251‑1 (MQ=255)                                                                                                                                       aGCGCAACTGGCGCGTACCAGTGTCCCTGTTCGTGCACAAAG‑GCACGGCAGAGCTGGATTCGAGTACCCTCGAACTGAGGGAAGAAGGTGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGaa                                                                                              <  2:118366/251‑1 (MQ=255)                                                                                                                                        gcgcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTTCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAt                                                                                             <  1:355593/251‑1 (MQ=255)                                                                                                                                          gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                                                           <  1:231656/251‑1 (MQ=255)                                                                                                                                           ccccTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACAAGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                                                          <  2:225673/249‑1 (MQ=255)                                                                                                                                           cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTACGGAATTTg                                                                                          >  2:415489/1‑251 (MQ=255)                                                                                                                                             ccTGGGGCGTACCGATGGCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGcc                                                                                        <  1:360500/251‑1 (MQ=255)                                                                                                                                                    cgtacctaggtcactgtgccggCACAAAG‑ACACGGAAGAGCTGCATCCTCGGACCCGCGAACTGATGGAAGAAGTGGCAAAGCGCGTTGAAG‑T‑TATCGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACTAATCTGACCAGTAGGTGAAAGTGCCGGATACTCTCGACGTATGGTTGGATTCCGGATCTTCCCACTTTTCTTTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                 <  2:463813/230‑1 (MQ=255)                                                                                                                                                     gtACCGATGTCACTGCTCGGGCTCAAGG‑ACTCTGTAGTGTGGCATCCGCGTACACCCGAACTGATGGGAGAAGTGGCAAAGCGCGTTGAAG‑TTAAC‑TGCATCCAGGCGGGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGAGCCTGATACTCTCTTCGTAGGGTGCGACTCCGGAGCTACCCACTCGTCTGTTGGTGACGTGCGTCCGGAATTTGCCggctacgc                                                                                <  2:458354/251‑7 (MQ=255)                                                                                                                                                         cGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCg                                                                            >  1:119022/1‑251 (MQ=255)                                                                                                                                                             gTCACTGTTAGAGCACAAAG‑AAACGGAAGAGATGCCTCCGCGTACCCTCGACCTGCTGGAAGAAGTGGCAAACCGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTCTGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGCCGTGAGTCCGGAATTTGCCGGTCACGCAGCGGAcc                                                                        >  2:500538/1‑250 (MQ=255)                                                                                                                                                              tCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                       >  1:213132/1‑251 (MQ=255)                                                                                                                                                               cACTGTTCGTGCACAAAT‑GCACCGAAGATCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACAt                                                                       <  1:30699/250‑1 (MQ=255)                                                                                                                                                               cACTGTTCGTGCACAAAG‑ACTCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGAGCTCGATGCGAAAGAGATCCTCGGCGACGAAGTTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATg                                                                      <  2:177918/251‑1 (MQ=255)                                                                                                                                                                   ggtCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCGTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTa                                                                    <  2:137850/247‑1 (MQ=255)                                                                                                                                                                     tCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTg                                                                >  1:289218/1‑251 (MQ=255)                                                                                                                                                                            caAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGCCCGGAATTTGCCGGTAACGCAGCGGACATGTATCTGGAAGGtt                                                         <  1:441694/251‑1 (MQ=255)                                                                                                                                                                             aaaG‑ACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCCTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAACGGCCATGTATCTGGAAGGTTc                                                        >  2:131690/1‑251 (MQ=255)                                                                                                                                                                                         agagCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGTAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGTCGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGCATTTGCCGGTCACGCAGCGGCCATGTATCTGGTAGGTTCTGAcccgcacc                                             >  2:150548/1‑245 (MQ=255)                                                                                                                                                                                          gagCTGCATCCGCGTACCCTCGAACTGATGTAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCTGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcg                                            <  1:23998/251‑1 (MQ=255)                                                                                                                                                                                               gCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggtt                                       <  1:262738/251‑6 (MQ=255)                                                                                                                                                                                                 aTCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttgg                                     <  1:385456/251‑8 (MQ=255)                                                                                                                                                                                                        tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCCGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATgg                              >  2:11691/1‑250 (MQ=255)                                                                                                                                                                                                         aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                              <  1:371679/250‑1 (MQ=255)                                                                                                                                                                                                         aCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc                             >  1:7771/1‑251 (MQ=255)                                                                                                                                                                                                          cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTc                             <  1:502111/250‑1 (MQ=255)                                                                                                                                                                                                          cccTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCt                            >  1:366720/1‑251 (MQ=255)                                                                                                                                                                                                            cgcgAACTGATGGAAGAAGGGGCCAAACGCGTTGCAG‑GTTAC‑GGCATCCAGGCGTGGTGGGATCTATATGCGAAAGAGATCCTCGGCGAAGAAGCTGACAAGTATGTGAAATTGCCGGATACTCTCGACGTATGGTTTGCCTCCGGATCTACCCACTCTGCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCTGCACCGTGGTTGGTTCATGTCtgc                          <  2:373539/248‑3 (MQ=255)                                                                                                                                                                                                              cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                        >  1:8109/2‑251 (MQ=255)                                                                                                                                                                                                               gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTAccc                                                                                                                           <  1:470990/151‑1 (MQ=255)                                                                                                                                                                                                               gAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTAcac                                                                                                                           >  2:470990/1‑149 (MQ=255)                                                                                                                                                                                                                          agaagTGGCAAAACGCGTTGCAG‑TTAAC‑GGCATCCAGGCGTAGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTCGACGTATGGATTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACAGTGGTTGGTTGATGTATTCCCTAATGATCTccc            >  2:333189/1‑250 (MQ=255)                                                                                                                                                                                                                           gaagTGGCAAAACGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCAc           <  1:73381/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCGCGTTGAAG‑TTAAC‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa  <  1:170297/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGt                                                          <  2:144464/188‑1 (MQ=255)                                                                                                                                                                                                                                           ttGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGt                                                          >  1:144464/1‑188 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           GTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGAC‑TGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAG‑ACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAG‑TCGAT‑GGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTGGATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAA  >  NC_000913/23657‑24132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑